LP1 from Lentinula edodes C91-3 Induces Autophagy, Apoptosis and Reduces Metastasis in Human Gastric Cancer Cell Line SGC-7901
Abstract
:1. Introduction
2. Results
2.1. LP1 Inhibits Cell Viability and Cell Proliferation
2.2. LP1 Arrested S Phase of the Cell Cycle in SGC-7901 Cells
2.3. LP1 Induced Autophagy in SGC-7901 and BGC-823 Cell Lines
2.4. LP1 Inhibited Migration, Invasion and Wound Healing in SGC-7901 Cells
2.5. LP1 Induced Apoptosis by Inhibition of PI3K/Akt/mTOR Pathway
3. Discussion
4. Materials and Methods
4.1. Reagents and Cell Culture
4.2. Cell Viability and Proliferation
4.3. Phase Contrast Microscopy
4.4. Cell Cycle Analysis
4.5. Electron Microscopy
4.6. Acridine Orange Staining
4.7. Cell Wound Healing Assay
4.8. Cell Invasion and Migration Assay
4.9. Annexin V-FITC and Propidium Iodide Staining
4.10. Western Blotting
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Batool, S.; Joseph, T.P.; Hussain, M.; Vuai, M.S.; Khinsar, K.H.; Din, S.R.U.; Padhiar, A.A.; Zhong, M.; Ning, A.; Zhang, W.; et al. LP1 from Lentinula edodes C91-3 Induces Autophagy, Apoptosis and Reduces Metastasis in Human Gastric Cancer Cell Line SGC-7901. Int. J. Mol. Sci. 2018, 19, 2986. https://doi.org/10.3390/ijms19102986
Batool S, Joseph TP, Hussain M, Vuai MS, Khinsar KH, Din SRU, Padhiar AA, Zhong M, Ning A, Zhang W, et al. LP1 from Lentinula edodes C91-3 Induces Autophagy, Apoptosis and Reduces Metastasis in Human Gastric Cancer Cell Line SGC-7901. International Journal of Molecular Sciences. 2018; 19(10):2986. https://doi.org/10.3390/ijms19102986
Chicago/Turabian StyleBatool, Samana, Thomson Patrick Joseph, Mushraf Hussain, Miza S. Vuai, Kavish. H. Khinsar, Syed Riaz Ud Din, Arshad Ahmed Padhiar, Mintao Zhong, Anhong Ning, Wei Zhang, and et al. 2018. "LP1 from Lentinula edodes C91-3 Induces Autophagy, Apoptosis and Reduces Metastasis in Human Gastric Cancer Cell Line SGC-7901" International Journal of Molecular Sciences 19, no. 10: 2986. https://doi.org/10.3390/ijms19102986