Mesoporous Silica-Bioglass Composite Pellets as Bone Drug Delivery System with Mineralization Potential
Abstract
:1. Introduction
2. Results and Discussion
2.1. DOX Adsorption onto MCM-41
2.2. Physicochemical Characterization of Pellets
2.3. Drug Release Profiles
2.4. Antimicrobial Activity of Pellets
2.5. Mineralization Potential Assay
2.6. Cytotoxicity Assay of Pellets
3. Materials and Methods
3.1. Synthesis of Bioglass
3.2. Synthesis of Mesoporous Silica MCM-41
3.3. Adsorption of Doxycycline Hydrochloride onto the MCM-41
3.4. Preparation of Pellets Composed of BG and MCM-DOX
3.5. Drug Release Studies
3.6. Antimicrobial Activity Assay
3.7. Mineralization Potential Assay
3.8. Cytotoxicity Assay
3.9. Physicochemical Characterization Methods
3.9.1. Fourier Transform Infrared Spectroscopy Analysis
3.9.2. Differential Scanning Calorimetry Analysis
3.9.3. Powder X-ray Diffraction Analysis
3.9.4. Stereoscopic Microscopy
3.9.5. Scanning Electron Microscopy with Energy Dispersive X-ray Spectroscopy
3.9.6. Yield of Pelletization Process
3.9.7. Size Analysis
3.9.8. Friability Test
3.9.9. Hardness Test
3.9.10. Drug Content Test
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yield (%) | Hardness (N) | Friability (%) | Drug Content (%) |
---|---|---|---|
78 ± 3 | 5.5 ± 1.3 | 1.1 ± 0.3 | 91.2 ± 3.5 |
Higuchi Model | Korsmeyer–Peppas Model | Zero-Order Kinetics * | |||
---|---|---|---|---|---|
kH | R2 | n | R2 | k0 | R2 |
7.3 | 0.991 | 0.36 | 0.987 | 2.4 | 0.972 |
Day | CFU/mL | Log10 Difference | Percent of Killed Bacteria | |
---|---|---|---|---|
Control | Pellets | |||
1 | 1.4 × 109 | 1.4 × 105 | 4.0 | >99.9% |
2 | 1.8 × 109 | 6.2 × 103 | 5.5 | |
3 | 4.7 × 109 | 4.5 × 104 | 5.0 | |
4 | 5.4 × 109 | 8.0 × 104 | 4.8 | |
5 | 6.5 × 109 | 1.5 × 105 | 4.6 | |
6 | 2.0 × 1010 | 1.0 × 105 | 5.3 | |
7 | 1.0 × 1010 | 3.5 × 105 | 4.5 |
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Szewczyk, A.; Skwira, A.; Konopacka, A.; Sądej, R.; Prokopowicz, M. Mesoporous Silica-Bioglass Composite Pellets as Bone Drug Delivery System with Mineralization Potential. Int. J. Mol. Sci. 2021, 22, 4708. https://doi.org/10.3390/ijms22094708
Szewczyk A, Skwira A, Konopacka A, Sądej R, Prokopowicz M. Mesoporous Silica-Bioglass Composite Pellets as Bone Drug Delivery System with Mineralization Potential. International Journal of Molecular Sciences. 2021; 22(9):4708. https://doi.org/10.3390/ijms22094708
Chicago/Turabian StyleSzewczyk, Adrian, Adrianna Skwira, Agnieszka Konopacka, Rafał Sądej, and Magdalena Prokopowicz. 2021. "Mesoporous Silica-Bioglass Composite Pellets as Bone Drug Delivery System with Mineralization Potential" International Journal of Molecular Sciences 22, no. 9: 4708. https://doi.org/10.3390/ijms22094708