Nitric Oxide Mobilizes Intracellular Zn2+ via the GC/cGMP/PKG Signaling Pathway and Stimulates Adipocyte Differentiation
Abstract
:1. Introduction
2. Results
2.1. Effect of ZnCl2 on Cell Viability and Adipocyte Differentiation
2.2. Effect of NO on Cell Viability and Adipocyte Differentiation
2.3. Effects of ZnCl2 and NO on Intracellular Zn2+ Mobilization
2.4. Role of Zn2+ in NO-Stimulated Adipocyte Differentiation
2.5. Correlation between Adipocyte Size, Zn2+ Level, and NOS Expression in Human Adipose Tissue
3. Discussion
4. Material and Methods
4.1. Materials
4.2. Experimental Design
4.3. 3T3-L1 Fibroblast Cell Culture and Differentiation Conditions
4.4. BODIPY 493/503 Staining
4.5. Measurement of Triglyceride
4.6. Immunoblot Analysis
4.7. MTT Assay
4.8. Live-cell Imaging of Intracellular Zinc
4.9. Collection of Human Subcutaneous Adipose Tissues
4.10. Real-time Polymerase Chain Reaction (RT-PCR)
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chen, C.-W.; Chen, L.-K.; Huang, T.-Y.; Yang, D.-M.; Liu, S.-Y.; Tsai, P.-J.; Chen, T.-H.; Lin, H.-F.; Juan, C.-C. Nitric Oxide Mobilizes Intracellular Zn2+ via the GC/cGMP/PKG Signaling Pathway and Stimulates Adipocyte Differentiation. Int. J. Mol. Sci. 2022, 23, 5488. https://doi.org/10.3390/ijms23105488
Chen C-W, Chen L-K, Huang T-Y, Yang D-M, Liu S-Y, Tsai P-J, Chen T-H, Lin H-F, Juan C-C. Nitric Oxide Mobilizes Intracellular Zn2+ via the GC/cGMP/PKG Signaling Pathway and Stimulates Adipocyte Differentiation. International Journal of Molecular Sciences. 2022; 23(10):5488. https://doi.org/10.3390/ijms23105488
Chicago/Turabian StyleChen, Chien-Wei, Luen-Kui Chen, Tai-Ying Huang, De-Ming Yang, Shui-Yu Liu, Pei-Jiun Tsai, Tien-Hua Chen, Heng-Fu Lin, and Chi-Chang Juan. 2022. "Nitric Oxide Mobilizes Intracellular Zn2+ via the GC/cGMP/PKG Signaling Pathway and Stimulates Adipocyte Differentiation" International Journal of Molecular Sciences 23, no. 10: 5488. https://doi.org/10.3390/ijms23105488