Assessment of the Antibiofilm Performance of Chitosan-Based Surfaces in Marine Environments
Abstract
:1. Introduction
2. Results
2.1. Chitosan and Its Mechanism of Action
2.2. Synthesis and Characterization of Poly (Lactic Acid)-Chitosan Surfaces
2.3. Antifouling Activity of CS-Based Surfaces
3. Discussion
4. Materials and Methods
4.1. Chitosan and Its Derivates
4.2. Bacteria Strains and Culture Conditions
4.3. Characterization of CS Mechanism of Action
4.4. Functionalization of Poly (Lactic Acid)-Chitosan Surfaces
4.5. Surface Characterization
4.5.1. Water Contact Angle Measurements
4.5.2. White Light Profilometry
4.6. Antifouling Activity of Chitosan-Based Surfaces
4.6.1. Biofilm Formation
4.6.2. Biofilm Quantification
Colony-Forming Unit Counts
Biofilm Thickness and Structure
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lima, M.; Gomes, L.C.; Teixeira-Santos, R.; Romeu, M.J.; Valcarcel, J.; Vázquez, J.A.; Cerqueira, M.A.; Pastrana, L.; Bourbon, A.I.; de Jong, E.D.; et al. Assessment of the Antibiofilm Performance of Chitosan-Based Surfaces in Marine Environments. Int. J. Mol. Sci. 2022, 23, 14647. https://doi.org/10.3390/ijms232314647
Lima M, Gomes LC, Teixeira-Santos R, Romeu MJ, Valcarcel J, Vázquez JA, Cerqueira MA, Pastrana L, Bourbon AI, de Jong ED, et al. Assessment of the Antibiofilm Performance of Chitosan-Based Surfaces in Marine Environments. International Journal of Molecular Sciences. 2022; 23(23):14647. https://doi.org/10.3390/ijms232314647
Chicago/Turabian StyleLima, Marta, Luciana C. Gomes, Rita Teixeira-Santos, Maria J. Romeu, Jesus Valcarcel, José Antonio Vázquez, Miguel A. Cerqueira, Lorenzo Pastrana, Ana I. Bourbon, Ed D. de Jong, and et al. 2022. "Assessment of the Antibiofilm Performance of Chitosan-Based Surfaces in Marine Environments" International Journal of Molecular Sciences 23, no. 23: 14647. https://doi.org/10.3390/ijms232314647