Reduced Levels of Selenium and Thioredoxin Reductase in the Thoracic Aorta Could Contribute to Aneurysm Formation in Patients with Marfan Syndrome
Abstract
:1. Introduction
2. Results
2.1. Demographic Characteristics of the MFS Patients
2.2. Inflammatory Interleukins, Se, and Carbonylation Levels
2.3. Enzymatic Activities and Expressions
2.4. Immunohistochemistry
3. Discussion
4. Materials and Methods
4.1. Ethical Considerations
4.2. MFS Patients and Control Subjects
4.3. Histology and Immunohistochemistry
4.4. Thoracic Aortic Aneurysm Tissue Homogenization
4.5. TrxR and GPx Activities
4.6. Oxidative Stress Markers
4.7. Determinations of IL-1β, TNF, IL-6 and TGF-β1
4.8. Determinations of NOX1 and NOS2 by Western Blotting
4.9. Statistical Analysis
5. Conclusions
Study Limitations
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patients | Age | Sex | HFH | AD | EL | SS | Total Systemic Score | Gen FBN1 | Total Ghent Criteria |
---|---|---|---|---|---|---|---|---|---|
1 | 38 | M | + | + | 10 | + | 3 | ||
2 | 29 | M | + | + | + | + | 14 | 4 | |
3 | 33 | M | + | + | + | + | 16 | 4 | |
4 | 56 | F | + | + | 12 | 2 | |||
5 | 20 | M | + | + | + | 18 | 3 | ||
6 | 25 | F | + | + | 7 | 2 | |||
7 | 27 | F | + | + | + | 8 | + | 4 | |
8 | 36 | F | + | + | + | 12 | 3 | ||
9 | 41 | F | + | + | + | 12 | 3 | ||
10 | 37 | F | + | + | + | 12 | 3 | ||
11 | 27 | M | + | + | + | 9 | 3 | ||
12 | 51 | M | + | + | 12 | + | 3 | ||
13 | 61 | F | + | + | + | 13 | + | 4 | |
14 | 29 | F | + | + | + | + | 12 | 4 | |
15 | 17 | M | + | + | + | 12 | 3 | ||
16 | 31 | M | + | + | + | 14 | 3 | ||
17 | 60 | M | + | + | + | + | 11 | 4 | |
18 | 45 | M | + | + | 10 | + | 3 | ||
19 | 43 | M | + | + | + | 7 | 3 |
Total n = 38 (100%) | MFS n = 19 (50%) | CS n = 19 (50%) | p | |
---|---|---|---|---|
Demographic variables in mean ± SE | ||||
Age | 39 ± 15 | 35 ± 12 | 43 ± 17 | NS |
Weight | 75 ± 17 | 75 ± 13 | 75 ± 20 | NS |
Size | 1.70 ± 0.13 | 1.77 ± 0.09 | 1.63 ± 0.11 | 0.0001 |
BMI | 25.6 ± 5.1 | 23.7 ± 3.4 | 27.5 ± 5.9 | 0.01 |
Cholesterol | 150.2 ± 48.9 | 148.7 ± 45.2 | 151.7 ± 53.3 | NS |
C-HDL | 39.2 ± 11.6 | 37.3 ± 13.2 | 41.2 ± 9.6 | NS |
C-LDL | 97.7 ± 33.3 | 97.7 ± 30.5 | 97.8 ± 43.8 | NS |
Triglycerides | 146 ± 82 | 146 ± 79 | 145 ± 86 | NS |
Glucose | 102 ± 26 | 100 ± 28 | 104 ± 25 | NS |
Creatinine | 0.88 ± 0.44 | 0.93 ± 0.56 | 0.83 ± 0.29 | NS |
Comorbidities (%) | ||||
Diabetes Mellitus | 3 (7) | 0 | 3 (13) | 0.07 |
SAH | 16 (33) | 5 (21) | 11 (46) | 0.06 |
Hypothyroidism | 1 (2) | 0 | 1 (4) | NS |
Alcoholism | 13 (27) | 7 (29) | 6 (25) | NS |
Smoking | 19 (40) | 10 (42) | 9 (38) | NS |
Echocardiography evaluation (% or median with the Min–Max or mean ± SE) | ||||
LVEF | 54 ± 11 | 50 ± 11 | 57 ± 11 | 0.02 |
Surgical | ||||
Aneurysm | 22 (49) | 11 (46) | 21 (88) | 0.002 |
Dissection | 16 (67) | 13 (54) | 3 (13) | 0.002 |
Clamp-Time | 143 (46–253) | 154 (46–253) | 128 (48–250) | NS |
Extracorporeal circulation | 187 (93–440) | 197 (148–336) | 177 (93–440) | NS |
EURO-SCOREII | 3 (4–17) | 3 (4–12) | 2 (5–17) | NS |
Variables (mg of Protein) | CS Median (Min–Max) | MFS Median (Min–Max) |
---|---|---|
IL-6 (pg) | 227.1 (0.0–888.96 | 409.1 (111.3–1137.1) *** |
TGF-β1 (pg) | 570.3 (21.7–1076.3) | 964.6 (384.2–3086.6) ** |
Carbonylation (nmol carbonyls) | 2.3 (0.8–3.6) | 2.5 (1.7–7.5) † |
Se (pg) | 224.2 (183.2–322.4) | 205.5 (24.4–243.9) * |
Thiols (µM) | 196.4 (119.1–286.5) | 164.8 (58.2–246.7) * |
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Soto, M.E.; Pérez-Torres, I.; Manzano-Pech, L.; Soria-Castro, E.; Morales-Marín, A.; Ramírez-Marroquín, E.S.; Martínez-Hernández, H.; Herrera-Alarcón, V.; Guarner-Lans, V. Reduced Levels of Selenium and Thioredoxin Reductase in the Thoracic Aorta Could Contribute to Aneurysm Formation in Patients with Marfan Syndrome. Int. J. Mol. Sci. 2023, 24, 10429. https://doi.org/10.3390/ijms241310429
Soto ME, Pérez-Torres I, Manzano-Pech L, Soria-Castro E, Morales-Marín A, Ramírez-Marroquín ES, Martínez-Hernández H, Herrera-Alarcón V, Guarner-Lans V. Reduced Levels of Selenium and Thioredoxin Reductase in the Thoracic Aorta Could Contribute to Aneurysm Formation in Patients with Marfan Syndrome. International Journal of Molecular Sciences. 2023; 24(13):10429. https://doi.org/10.3390/ijms241310429
Chicago/Turabian StyleSoto, María Elena, Israel Pérez-Torres, Linaloe Manzano-Pech, Elizabeth Soria-Castro, Almilcar Morales-Marín, Edgar Samuel Ramírez-Marroquín, Humberto Martínez-Hernández, Valentín Herrera-Alarcón, and Verónica Guarner-Lans. 2023. "Reduced Levels of Selenium and Thioredoxin Reductase in the Thoracic Aorta Could Contribute to Aneurysm Formation in Patients with Marfan Syndrome" International Journal of Molecular Sciences 24, no. 13: 10429. https://doi.org/10.3390/ijms241310429