Metalloproteomic Investigation of Hg-Binding Proteins in Renal Tissue of Rats Exposed to Mercury Chloride
Abstract
:1. Introduction
2. Results and Discussion
2.1. Hg-Determinations
2.2. Renal Proteome Fractionation
2.3. Characterization of Hg-Associated Protein Spots by LC–MS/MS
2.4. Analysis Using the Shotgun-LC–MS/MS Strategy
3. Material and Methods
3.1. Animal Experimentation
3.2. Renal Proteome Fractionation by 2D PAGE
3.3. Hg Determinations by GFAAS
3.4. Analysis by LC–MS/MS
3.4.1. Characterization of Hg-Associated Protein Spots
3.4.2. Expression Analysis by Shotgun–LC–MS/MS
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Samples | Hg Concentration (µg kg−1) |
---|---|
Hg-30 Tissue | 39.20 ± 0.71 |
Hg-60 Tissue | 60.40 ± 1.10 |
Hg-30 Pellet | 36.50 ± 0.62 |
Hg-60 Pellet | 55.60 ± 0.98 |
Fish Liver DOLT 4 | 2.530 ± 0.060 * |
Spot ID | Access | Proteins ID | Score | [Hg] (mg kg−1) |
---|---|---|---|---|
7 | Albumin | 58.669 | 120 ± 2 | |
10 | - | Not identified | - | 113 ± 2 |
13 | P11517 P02091 | Hemoglobin subunit beta-2 Hemoglobin subunit beta-1 | 272.2158 272.2158 | 138 ± 2 |
14 | - | Not identified | - | 293 ± 4 |
18 | - | Not identified | - | 302 ± 5 |
20 | - | Not identified | - | 316 ± 5 |
21 | - | Not identified | - | 417 ± 6 |
22 | P63259 P60711 P68136 P68035 P63269 P62738 | Actin_ cytoplasmic 2 Actin_ cytoplasmic 1 Actin_ alpha skeletal muscle Actin_ alpha cardiac muscle 1 Actin_ gamma-enteric smooth muscle Actin_ aortic smooth muscle | 510.8116 510.8116 142.5171 142.5171 142.5171 1425171 | 750 ± 9 |
31 | P02761 | Major urinary protein | 212.1593 | 349 ± 5 |
35 36 | P02091 P11517 | Hemoglobin subunit beta-1 Hemoglobin subunit beta-2 | 333.9544 272.685 | 84 ± 1 |
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de Almeida, E.C.; Faria, V.D.; Cirinêu, F.D.; Santiago, M.G.A.; Miotto, B.; Vieira, J.C.S.; Braga, C.P.; Adamec, J.; Fernandes, A.A.H.; Buzalaf, M.A.R.; et al. Metalloproteomic Investigation of Hg-Binding Proteins in Renal Tissue of Rats Exposed to Mercury Chloride. Int. J. Mol. Sci. 2024, 25, 164. https://doi.org/10.3390/ijms25010164
de Almeida EC, Faria VD, Cirinêu FD, Santiago MGA, Miotto B, Vieira JCS, Braga CP, Adamec J, Fernandes AAH, Buzalaf MAR, et al. Metalloproteomic Investigation of Hg-Binding Proteins in Renal Tissue of Rats Exposed to Mercury Chloride. International Journal of Molecular Sciences. 2024; 25(1):164. https://doi.org/10.3390/ijms25010164
Chicago/Turabian Stylede Almeida, Emerson Carlos, Victor Diego Faria, Felipe Dalmazzo Cirinêu, Maria G. A. Santiago, Beatriz Miotto, José C. S. Vieira, Camila Pereira Braga, Jiri Adamec, Ana A. H. Fernandes, Marília A. R. Buzalaf, and et al. 2024. "Metalloproteomic Investigation of Hg-Binding Proteins in Renal Tissue of Rats Exposed to Mercury Chloride" International Journal of Molecular Sciences 25, no. 1: 164. https://doi.org/10.3390/ijms25010164