Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Zinc Finger by CD Spectroscopy
2.2. Evaluation of the Binding between NCp7 ZF and Natural Compounds by Fluorescence Assay
2.3. Elucidation of the Binding Mode between NCp7 ZF and Natural Compounds by Mass Spectrometry
2.4. Structural Information of the Binding from CD Spectroscopy and Molecular Docking
3. Materials and Methods
3.1. Reagents and Equipment
3.2. Methods and Procedure
3.2.1. Preparation of Zinc Finger Protein
3.2.2. Fluorescence Assay
3.2.3. Mass Spectrometry
3.2.4. Docking
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
Abbreviations
ZF | zinc finger |
LQ | liquiritin |
ILQ | isoliquiritin |
GL | glycyrrhizic acid |
GA | glycyrrhetinic acid |
CUR | curcumin |
HIV-NCp7 | the human immunodeficiency virus nucleocapsid protein |
NCIs | NC inhibitors |
CD | circular dichroism |
ESI-MS | electrospray ionization-mass spectrometer |
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Species | Formula | Mono Observed m/z |
---|---|---|
ZF | C90H144N30O28S4Zn | 762.62 |
ZF–ILQ adduct | C111H166N30O37S4Zn | 902.02 |
Oxipeptide–ILQ | C111H166N30O37S4 | 880.71 |
ZF–CUR adduct | C111H164N30O37S4Zn | 885.35 |
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Wang, R.; Wei, Y.; Wang, M.; Yan, P.; Jiang, H.; Du, Z. Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity. Molecules 2021, 26, 3563. https://doi.org/10.3390/molecules26123563
Wang R, Wei Y, Wang M, Yan P, Jiang H, Du Z. Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity. Molecules. 2021; 26(12):3563. https://doi.org/10.3390/molecules26123563
Chicago/Turabian StyleWang, Runjing, Yinyu Wei, Meiqin Wang, Pan Yan, Hongliang Jiang, and Zhifeng Du. 2021. "Interaction of Natural Compounds in Licorice and Turmeric with HIV-NCp7 Zinc Finger Domain: Potential Relevance to the Mechanism of Antiviral Activity" Molecules 26, no. 12: 3563. https://doi.org/10.3390/molecules26123563