Comparative Analysis of Polycyclic Aromatic Hydrocarbons and Halogenated Polycyclic Aromatic Hydrocarbons in Different Parts of Perilla frutescens (L.) Britt.
Abstract
:1. Introduction
2. Results and Discussion
2.1. Method Optimization and Validation
2.2. Comparative Analysis
2.3. Health Risk Assessment
3. Materials and Methods
3.1. Materials and Reagents
3.2. GC-MS Conditions
3.3. Sample Preparation
3.3.1. The Preparation of Perilla Leaves and Stems
3.3.2. The Preparation of Perilla Seeds
3.4. Method Verification
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Name | Abbreviation | Rt (min) | Quantitative Ion | Qualitative Ion | Quantitative Internal Standard |
---|---|---|---|---|---|
Naphthalene-d8 | Nap-d8 | 12.4 | 136.1 | 137.1, 108.0 | \ |
Naphthalene | Nap | 12.5 | 128.0 | 127.0, 101.9 | Nap-d8 |
Phenanthrene-d10 | Ace-d10 | 20.3 | 164.1 | 162.1, 160.1 | \ |
Acenaphthylene | Acy | 19.6 | 152.0 | 151.0, 76.0, 63.0 | Ace-d10 |
Acenaphthene | Ace | 20.5 | 154.0 | 152.0, 76.0 | Ace-d10 |
Fluorene | Fle | 22.8 | 166.0 | 165.0, 139.0 | Ace-d10 |
Acenaphthene-d10 | Phe-d10 | 26.0 | 188.1 | 160.1, 94.1 | \ |
Phenanthrene | Phe | 26.1 | 178.1 | 89.1, 152.0 | Phe-d10 |
Anthracene | Ant | 26.3 | 178.1 | 89.1, 152.0 | Phe-d10 |
2-Bromofluorene | 2-BrFle | 27.8 | 165.0 | 244.0, 245.9, 162.9 | Phe-d10 |
2,7-Dichlorofluorene | 2,7-Cl2Fle | 29.5 | 199.0 | 234.0, 162.9, 200.9 | Phe-d10 |
Fluoranthene | Flu | 30.1 | 202.0 | 200.0, 100.9, 88.0 | Phe-d10 |
Pyrene | Pyr | 31.0 | 202.0 | 100.9, 200.0 | Phe-d10 |
9-Bromoanthracene | 9-BrAnt | 31.1 | 258.0 | 177.0, 176.0, 88.0 | Phe-d10 |
Chrysene-d12 | Chr-d12 | 37.9 | 240.1 | 236.1, 120.1 | \ |
9,10-Dibromoanthracene | 9,10-Br2Ant | 37.7 | 336.0 | 176.0, 88.1 | Chr-d12 |
Benzo(a)anthracene | Baa | 37.9 | 228.0 | 225.9, 114.1 | Chr-d12 |
Chrysene | Chr | 38.1 | 228.0 | 225.9, 114.1 | Chr-d12 |
Perylene-d12 | Per-d12 | 48.9 | 264.1 | 260.1, 265.1 | \ |
Benzo(b)fluoranthene | BbF | 46.0 | 252.0 | 250.1, 126.0, 112.8 | Per-d12 |
Benzo(k)fluoranthene | BkF | 46.2 | 252.0 | 250.1, 126.0, 112.8 | Per-d12 |
Benzo(a)pyrene | BaP | 48.4 | 252.0 | 250.0, 126.0 | Per-d12 |
Indeno(1,2,3-c,d)pyrene | IncdP | 56.0 | 276.0 | 138.0, 277.0 | Per-d12 |
Dibenzo(a,h)anthracene | DahA | 56.3 | 278.0 | 279.0, 138.8 | Per-d12 |
Benzo(g,h,i)perylene | BghiP | 57.2 | 276.0 | 137.1, 138.0, 274.0 | Per-d12 |
NO. | Perilla Leaves (A) | Perilla Stems (B) | Perilla Seeds (C) | |||
---|---|---|---|---|---|---|
TEQ | ILCR | TEQ | ILCR | TEQ | ILCR | |
1 | 0.05 | 4.05 × 10−8 | 0.02 | 1.66 × 10−8 | 0.02 | 1.88 × 10−8 |
2 | 0.04 | 3.30 × 10−8 | 0.02 | 1.68 × 10−8 | 0.08 | 6.24 × 10−8 |
3 | 0.17 | 1.32 × 10−7 | 0.03 | 2.13 × 10−8 | 0.03 | 2.34 × 10−8 |
4 | 0.18 | 1.45 × 10−7 | 0.03 | 2.36 × 10−8 | 0.04 | 3.06 × 10−8 |
5 | 0.22 | 1.70 × 10−7 | 0.04 | 3.21 × 10−8 | 0.18 | 1.41 × 10−7 |
6 | 0.19 | 1.53 × 10−7 | 0.02 | 1.60 × 10−8 | 0.06 | 4.42 × 10−8 |
7 | 0.09 | 7.01 × 10−8 | 0.02 | 1.36 × 10−8 | 0.07 | 5.31 × 10−8 |
8 | 0.30 | 2.38 × 10−7 | 0.01 | 6.82 × 10−9 | 0.08 | 6.51 × 10−8 |
9 | 0.38 | 2.97 × 10−7 | 0.01 | 5.52 × 10−9 | 0.05 | 3.97 × 10−8 |
10 | 0.67 | 5.29 × 10−7 | 0.03 | 2.23 × 10−8 | 0.07 | 5.81 × 10−8 |
11 | 2.28 | 1.79 × 10−6 | 0.04 | 2.80 × 10−8 | 0.12 | 9.81 × 10−8 |
12 | 0.64 | 5.02 × 10−7 | 0.07 | 5.50 × 10−8 | 0.04 | 3.35 × 10−8 |
13 | 0.25 | 2.00 × 10−7 | 0.04 | 3.02 × 10−8 | 0.06 | 4.75 × 10−8 |
14 | 0.09 | 7.35 × 10−8 | 0.03 | 2.21 × 10−8 | 0.02 | 1.20 × 10−8 |
15 | 0.36 | 2.84 × 10−7 | 0.04 | 3.24 × 10−8 | 0.14 | 1.11 × 10−7 |
16 | 18.18 | 1.43 × 10−5 | 0.03 | 2.25 × 10−8 | 0.12 | 9.15 × 10−8 |
17 | 19.22 | 1.51 × 10−5 | 0.03 | 2.47 × 10−8 | 0.05 | 3.98 × 10−8 |
18 | 19.15 | 1.50 × 10−5 | 0.06 | 4.46 × 10−8 | 0.05 | 3.94 × 10−8 |
19 | 20.37 | 1.60 × 10−5 | 0.04 | 3.32 × 10−8 | 0.04 | 2.93 × 10−8 |
20 | 0.29 | 2.29 × 10−7 | 0.04 | 2.90 × 10−8 | 0.05 | 3.83 × 10−8 |
21 | 26.89 | 2.11 × 10−5 | - | - | 0.12 | 9.65 × 10−8 |
22 | - | - | - | - | 0.03 | 2.25 × 10−8 |
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Wang, P.; Jin, B.; Lian, C.; Guo, K.; Ma, C. Comparative Analysis of Polycyclic Aromatic Hydrocarbons and Halogenated Polycyclic Aromatic Hydrocarbons in Different Parts of Perilla frutescens (L.) Britt. Molecules 2022, 27, 3133. https://doi.org/10.3390/molecules27103133
Wang P, Jin B, Lian C, Guo K, Ma C. Comparative Analysis of Polycyclic Aromatic Hydrocarbons and Halogenated Polycyclic Aromatic Hydrocarbons in Different Parts of Perilla frutescens (L.) Britt. Molecules. 2022; 27(10):3133. https://doi.org/10.3390/molecules27103133
Chicago/Turabian StyleWang, Pengfei, Bo Jin, Chaojie Lian, Kaijing Guo, and Chen Ma. 2022. "Comparative Analysis of Polycyclic Aromatic Hydrocarbons and Halogenated Polycyclic Aromatic Hydrocarbons in Different Parts of Perilla frutescens (L.) Britt." Molecules 27, no. 10: 3133. https://doi.org/10.3390/molecules27103133