Search Results (265)

Lycoris longituba produces a single flower bearing six tepals. The double-petaled phenotype of L. longituba has gained significant interest in China due to its ornamental and commercial value in tourism industries. This double-petal phenotype, characterized by stamen petalization, shows improved esthetic characteristics compared with conventional single-petal form. However, the molecular mechanisms underlying this floral trait remain largely undefined. In this study, RNA-based comparative transcriptomic analysis was performed between single- and double-petaled flowers of L. longituba at the fully opened flower stage. Approximately 13,848 differentially expressed genes (DEGs) were identified (6528 upregulated and 7320 downregulated genes). Functional annotation through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed several DEGs potentially involved in double-petal development. Six candidate genes, including the hub genes LlbHLH49, LlNAC1, LlSEP, LlTIFY, and LlAGL11, were identified based on DEG functional annotation and weighted gene co-expression network analysis (WGCNA). Transcription factors responsive to phytohormonal signaling were found to play a pivotal role in modulating double-petal development. Specifically, 123 DEGs were involved in phytohormone biosynthesis and signal transduction pathways, including those associated with auxin, cytokinin, gibberellin, ethylene, brassinosteroid, and jasmonic acid. Moreover, 521 transcription factors (TFs) were identified, including members of the MYB, WRKY, AP2/ERF, and MADS-box families. These results improve the current understanding of the genetic regulation of the double tepal trait in L. longituba and offer a base for future molecular breeding strategies to enhance ornamental characteristics. Full article

Structural analysis of glycosphingolipids provides novel insights into organismal classification and reveals conserved functional roles that transcend taxonomic boundaries. To elucidate the structural characteristics of acidic glycosphingolipids (AGLs) in the adductor muscle of the Japanese giant scallop (Patinopecten yessoensis), AGLs were isolated and purified by column chromatography using anion exchange resin and silica gel. Structural characterization was performed using mass spectrometry, proton nuclear magnetic resonance spectroscopy, and immunological techniques. The sugar chain structure was identified as GlcA4Meβ1-4(GalNAc3Meα1-3)Fucα1-4GlcNAcβ1-2Manα1-3Manβ1-4Glcβ1-Cer, consistent with the mollu-series core reported for mollusks. In addition to uronic acid, the structure was distinguished by internal fucose and methylated sugars, features commonly found in bivalves. The presence of xylose in the sugar chains of AGLs was also suggested. In contrast, the ceramide moiety was composed primarily of fatty acids C16:0 and C18:0 and the long-chain base d16:1. This chemical structure provides valuable insights into the biological classification of P. yessoensis and the mollu-series glycolipids containing fucose and methylated sugars, which may serve as bioactive components shared across species in the phylum Mollusca and class Bivalvia. Full article

The volumetric Hirst method is considered a golden standard in aerobiology for determining particle number concentrations of bioaerosols. Using Hirst-type pollen and spore traps on mobile platforms (i.e., aircraft, cars, motorbikes, bicycles or carried by pedestrians) is anticipated to significantly enhance the spatial and temporal granularity of data for bioaerosol monitoring. Mobile sampling promises to enhance our understanding of bioaerosol dynamics, ecological interactions and the impact of human activities on airborne biological particles. In this article, we present the design and test of an airborne Hirst-type volumetric sampler. We followed a structured approach and incorporated the fundamental principles of the original design, while optimizing for size, weight, power and cost. Our portable Hirst-type volumetric sampler (FlyHirst) was attached to an ultralight aircraft, together with complementing instrumentation, and was tested for collection of atmospheric concentrations of pollen, fungal spores and hyphae. By linking the temporal resolution of the samples with the spatial position of the aircraft, using flight time, we calculated the spatial resolution of our measurements in 3D. In six summer flights over Denmark, our study revealed that the diversity of the recorded spores corresponded to the seasonal expectance. Urtica pollen was recorded up to 1300 m above ground (a.g.l.), and fungal spores up to 2100 m a.g.l. We suggest that, based on this proof-of-concept, FlyHirst can be applied on other mobile platforms or as a personal sampler. Full article

To investigate the genetic regulatory mechanism of supplementary feeding on muscle development in Oula sheep, we employed transcriptomic analysis to explore the differentially expressed genes (DEGs) in the longissimus dorsi muscle of Oula sheep at different ages under conditions of supplementary feeding and non-supplementary feeding, as well as the significantly enriched Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathways of DEGs. Moreover, by combining with the method of weighted gene co-expression network analysis, we screened for the potential hub genes that might play crucial roles. The results demonstrated that the CD4 and ICAM1 genes and the PI3K-Akt signaling pathway might exert important functions during the lamb stage. At the growth stage, the AGL, PGM2L1, PRKAA2, NEDD4, and GBE1 genes might serve as core genes to regulate the growth of skeletal muscle in Oula sheep after supplementary feeding through signaling pathways such as starch and sucrose metabolism and insulin signaling pathway. This outcome provides a molecular-level interpretation of the regulatory mechanism of supplementary feeding on muscle growth and development in Oula sheep at different ages, offering a theoretical basis for the further improvement of the meat quality of Oula sheep and the enhancement of the quality of livestock products in the Qinghai–Tibet Plateau region. Full article

Background: The etiology of liver disease remains unidentified in approximately 30% of patients, presenting a persistent diagnostic challenge. While whole-exome sequencing (WES) is well established for identifying rare genetic conditions in pediatric populations, its utility in adult hepatology is less defined. This study aimed to evaluate the diagnostic value of WES in adults with unexplained liver disorder. Methods: Fifty-three Turkish adult patients with idiopathic liver disease underwent a comprehensive clinical evaluation and WES at Gazi University Ankara in 2024–2025. The cohort included individuals with idiopathic cholestasis (6/53, 11%), hepatic steatosis (28/53, 53%), unexplained elevated liver enzymes (12/53, 23%), and cryptogenic cirrhosis (7/53, 13%). All patients had inconclusive results from prior standard investigations. Results: ES yielded a definitive molecular diagnosis in 11% (6/53) of cases. Definitive diagnoses were distributed across the following disease categories: idiopathic cholestasis (n = 1), hepatic steatosis (n = 1), elevated liver enzymes (n = 2), and cryptogenic cirrhosis (n = 2). Pathogenic variants were detected in the ABCB4, AGL, APOB, CP, and MTTP genes. One patient was identified with mosaic Turner syndrome. Conclusions: This study highlights the role of rare genetic variants in the etiology of unexplained liver disease in adults. Integrating whole-exome sequencing into hepatology practice can uncover novel disease mechanisms and improve diagnostic yield, informing more precise patient care. Full article

This study examines a convergence event at the trailing end of a cold front observed in the United States’ Southern Great Plains region on 28 September 1997, using an array of in situ and remote sensing instruments. The event exhibited a structure with elevated divergence near 3 km AGL and moisture transport over both warm and cold sectors. Data from Raman lidar (RL), Atmospheric Emitted Radiance Interferometer (AERI), and Radar Wind Profilers (RWP) were used to characterize vertical profiles of the event, revealing the presence of a narrow moist updraft, horizontal moisture advection, and cloud development ahead of the front. Convection parameters, Convective Available Potential Energy (CAPE) and Convective Inhibition (CIN), were derived from collocated AERI and RL. Regions of high CAPE were aligned with areas of high moisture, indicating that convection was more favorable at moist elevated levels than near the surface. RWP observations revealed vorticity structures consistent with existing theories. This study highlights the value of high-resolution, continuous profiling from remote sensors to resolve mesoscale processes and evaluate convection potential. The event underscores the role of elevated moisture and wind shear in modulating convection initiation along a trailing-end cold front boundary where mesoscale and synoptic forces interact. Full article

FLOWERING LOCUS T (FT) is a key integrator of flowering pathways. White lupin, a grain legume, encodes four FT homologs: LalbFTa1, LalbFTa2, LalbFTc1, and LalbFTc2. Widespread distribution of white lupin implies diverse phenological adaptations to contrasting ecosystems. Recent studies highlighted associations between FT indels and flowering regulation. Therefore, we surveyed the global white lupin collection for the presence of such indels and potential links to phenology. A panel of 626 white lupin genotypes, representing several European and African agro-climates, was phenotyped under a long-day photoperiod in a two-year study, showing up to 80 days of flowering time difference between early landraces from Eastern Mediterranean and late accessions from France, Madeira, the Canaries, Greece, Italy, and the Azores. As many as seventeen indel variants were identified for LalbFTc1, twelve for LalbFTa2, nine for LalbFTa1, and four for LalbFTc2, yielding roughly three hundred allelic combinations. Significant correlations with phenology were confirmed for one LalbFTa1 indel and twelve LalbFTc1 indels. A large, highly correlated LalbFTc1 indel was revealed to be conserved among all domesticated Old World lupins, carrying all FTc1-promoter candidate binding sites of the same major floral repressor, AGAMOUS-LIKE 15. A small LalbFTa1 indel, providing additional contribution to earliness, showed homology between white and yellow lupins. LalbFTc1 indel-based PCR markers revealed high discriminatory power towards early (PR_42a and PR_71b) or late (PR_58c, PR_36b, PR_80, and PR_60b) flowering. Full article

Jatropha curcas L. is a shrub of the Euphorbiaceae family with non-toxic varieties found in Mexico that holds significant potential for biofuel production and other industrial applications. However, its limited in vitro regenerative capacity is a barrier to the development of productive species. Somatic embryogenesis (SE) offers a strategy to establish a regeneration system to overcome these challenges and enable genetic improvement. In this work, proteomic and gene expression analyses were utilized to identify key factors involved in SE induction in a non-toxic variety of J. curcas. Two-dimensional electrophoresis (2-DE) in combination with mass spectrometry was used to compare the proteomes of pre-globular and globular somatic embryos. RT-qPCR was used for gene expression analysis of the BBM, AGL15, SERK, IAA26 and eIF3f genes. The globular stage showed enrichment in the pathways related to carbohydrate and energy metabolism, protein folding, and stress response. In addition, the gene expression analysis of selected genes revealed a significantly elevated expression of BBM, AGL15, and IAA26 in globular embryos compared to pre-globular embryos. In contrast, SERK expression was low, and eIF3f expression remained unchanged between stages. These expression patterns may contribute to developmental arrest at the globular stage. These findings provide new insights into the molecular mechanisms regulating early SE in J. curcas and offer potential strategies for improving its propagation and industrial applications. Full article

Transition metal oxide aerogels (AGLs) have attracted considerable attention in recent years due to their exceptional properties, including high surface area, significant porosity, and ultralow density. In this study, we report the first-time synthesis of zinc oxide nano-wafers and zinc aerogels for application as supercapacitor electrodes. The aerogels were synthesized via a novel one-pot hydrolysis method using NaBH₄ as a reducing agent and subsequently annealed at 200 °C (Zn_AGL(200)) and 450 °C (Zn_AGL(450)) to investigate the influence of temperature on their electrochemical properties. Structural and morphological characterizations were conducted using XRD, FTIR, BET, XPS, SEM, and TEM analyses. Among the fabricated electrodes, the aerogel annealed at 200 °C (Zn_AGL(200)) exhibited superior energy storage performance, attributed to its amorphous, continuous network structure, which enhanced its surface area and reduced its density compared to both the as-synthesized (Zn_AGL(RT)) and 450 °C-annealed (Zn_AGL(450)) counterparts. A two-electrode device demonstrated excellent cycling stability over 10,000 cycles, achieving an energy density of 7.97 Wh/kg and a power density of 15 kW/kg. These findings highlight the potential of zinc aerogels as materials for next-generation lightweight energy storage systems, with promising applications in industrial, mechanical, and aerospace technologies. Full article

Snapdragon (Antirrhinum majus) serves as a model system for dissecting floral morphogenesis mechanisms. Petal malformation in A. majus impacts ornamental value, but its genetic basis remains poorly understood. We compared transcriptomes of the wild-type (Am11) and a petal-malformed mutant (AmDP2) to identify 2303 differentially expressed genes (DEGs), including E-class MIKC-type MADS-box genes SEP3 (AmMADS25/61/20/26) and SEP2 (AmMADS85). Weighted gene co-expression network (WGCNA), protein-protein interaction (PPI), qRT-PCR and virus-induced gene silencing (VIGS) analyses revealed interactions between SEP2/SEP3 and C/A/B-class MADS-box genes (AG, AP1, AP3), co-regulated MADS transcription factors (MTFs) AGL15 (AmMADS16), and auxin signaling genes (SAUR1, IAA13). qRT-PCR validated upregulation of SEP3 and downregulation of SEP2 in AmDP2. Our results suggest that E-class MADS-box genes are associated with petal malformation through coordinated interactions with hormonal pathways. These findings provide candidate targets for further functional studies in snapdragon. Full article

This study explores the potential of wind energy to address electricity shortages in South Africa, focusing on the Ekuphumleni community in Whittlesea. Given the challenges of expanding the national grid to these areas, wind energy is considered to be a feasible alternative to provide clean, renewable energy and reduce fossil fuel dependence in this community. This research evaluates wind potential utilizing the two-parameter Weibull distribution, with scale and shape parameters estimated by five traditional numerical methods and one metaheuristic optimization technique: whale optimization algorithm (WOA). Goodness-of-fit tests, such as the coefficient of determination (R²) and wind power density error (WPDE), were utilized to determine the best method for accurately estimating Weibull scale and shape parameters. Furthermore, net fitness, which combines R² and WPDE, was employed to provide a holistic assessment of overall performance. Whittlesea showed moderate wind speeds, averaging 3.88 m/s at 10 m above ground level (AGL), with the highest speeds in winter (4.87 m/s) and optimum in July. The WOA method outperformed all five numerical methods in this study in accurately estimating Weibull distribution parameters. Interestingly, the openwind method (OWM), a numerical technique based on iterative methods, and the Brent method showed comparable performance to WOA. The wind power density was 67.29 W/m², categorizing Whittlesea’s potential as poor and suitable for small-scale wind turbines. The east wind patterns favor efficient turbine placement. The study recommends using augmented wind turbines for the site to maximize energy capture at moderate speeds. Full article

The MIKC-type MADS-box (MIKC) gene family is essential for controlling various plant developmental processes, including flowering time and dormancy transitions. Although the MIKC gene family has been widely studied across different plants, its characterization and functional study in herbaceous peony remain limited. In this study, 19 Paeonia lactiflora Pall. MIKC-type (PlMIKC) genes were identified from the transcriptome of a low-chilling requirement Paeonia lactiflora Pall. cultivar ‘Hang Baishao’. These MIKC genes were categorized into seven clades: six were classified as MIKC^C-type, including FUL/AP1, DAM, PI, AGL18, AGL12, AG, and SOC1, and one, AGL30, was classified as MIKC*-type. Notably, the FLC clade genes were absent in Paeonia lactiflora Pall. The PlMIKC genes were predominantly localized to the nucleus, and their sequences contained highly conserved MADS and K-domains. Phylogenetic analysis demonstrated that PlMIKC genes share a strong evolutionary affinity with the MIKC genes from grapevine (Vitis vinifera) and poplar (Populus trichocarpa). A low-temperature-induced bud dormancy transition (BDT) experiment revealed that PlMIKC genes, such as PlFUL and PlDAM, were highly expressed during dormancy maintenance, while PlSOC1, PlAGL12, and PlAGL30 were upregulated during BDT. Additionally, the transient overexpression of PlSOC1 in ‘Hang Baishao’ significantly accelerated BDT and promoted bud break, suggesting that SOC1, traditionally linked to flowering regulation, also plays a key role in dormancy transition. Since limited literature on the MIKC gene family is currently available in herbaceous peony, this study expands the knowledge of the MIKC genes in Paeonia lactiflora Pall. and offers valuable insights into the molecular regulation of bud dormancy in response to low temperatures. Full article

Chinese cabbage (Brassica rapa L. ssp. pekinensis) is a key vegetable crop in Asia, but its commercial value is often reduced by premature flowering triggered by vernalization. The molecular mechanisms behind this process are not fully understood. MADS-box genes, as crucial transcriptional regulators, play vital roles in plant development, including flowering. In this study, 102 MADS-box genes were identified in Chinese cabbage through bioinformatics analyses, covering phylogeny, chromosomal localization, and gene structure. Real-time quantitative PCR and RNA-seq data analysis revealed that the expression level of AGL27 declined as vernalization time increased. To determine BrAGL27′s functions, we obtained BrAGL27-overexpressed (OE) Arabidopsis thaliana lines that showed significantly later flowering compared with the wild type (WT). The expression levels of flowering suppressor genes AtFLC and AtTEM1 were significantly high-regulated in the BrAGL27-OE lines compared to WT plants, while the expression levels of the floral genes AtSPL15, AtSOC1, AtFT, and AtAP3 were significantly lower in the BrAGL27-overexpressed lines than in the wild type. These findings enhance understanding of MADS-box genes in vernalization and flowering regulation, offering a basis for further research on bolting resistance and flowering control in Chinese cabbage. Full article

Rubber dandelion (Taraxacum kok-saghyz Rodin, TKS), also referred to as Russian dandelion, is one of the most promising natural rubber (NR)-producing plants that produce high-quality NR comparable to that from the Pará rubber tree (Hevea brasiliensis, Hb), currently the only commercial source. It needs further breeding to improve the agricultural traits. However, little has been known about the genetic mechanisms underlying the regulation of floral induction and flower development in TKS, an important trait that remains to be improved for commercial production. The MADS-box gene AGAMOUS-LIKE 24 (AGL24) plays important roles in floral induction and flower development. As the first step in understanding its roles in TKS, this study isolated and characterized the AGL24-homologous gene TkAGL24 in TKS. The TkAGL24 gene had a 705 bp coding sequence (CDS) that encoded a protein of 234 amino acids containing the conserved classic MADS-box type II domain and K-box domain, sharing 55.32% protein sequence identity with the AtAGL24 protein from Arabidopsis. TkAGL24 was highly expressed in leaf, latex, root, and peduncle but rarely or not in mature flower. The TkAGL24 protein was located in the nucleus and cytoplasm and did not have transcription activation activity in yeast cells. The overexpression of TkAGL24 in Arabidopsis could promote flowering and cause the abnormal development of flowers, similar to other AGL24-homologous genes from other species. Furthermore, the overexpression of TkAGL24 in TKS also affected the development of ligulate flowers. These results suggested that the cloned TkAGL24 gene is functional and may play important roles in floral induction and flower development in TKS, providing an insight into the possibility for the further studies of its roles and application to breeding. Full article

The availability of agricultural land is central to stimulating reserves in sustainable food and crop production amidst accelerating economic sustainability and growth. Therefore, this article aims to investigate the influence of agricultural land (AGL) on food production (FP) and crop production (CP) with the linkage of environmental sustainability (ES) as a moderator from 1990 to 2021 for the economy of China with the autoregressive distributed lag (ARDL) bounds testing estimation model. Our findings showed that the ARDL model estimates the long-term and short-term joint matching relationships between agricultural land and the independent variables in the model, which is a statistically significant outcome. Therefore, in the long term, the food and crop production adjustment for speed to steadiness was huge as it was projected at 1.337%, 53.6%, 133.5%, and 37.4%, respectively, in all the models, which shows that the adjustment for speed of models is a good post-shock association process. We found evidence for a significant and positive relationship between agricultural land and food and crop production in ordinary least square (OLS) estimation, which also ensured the outcomes of the primary model. Furthermore, Toda–Yamamoto Granger causality test estimation found reverse causality between food production (FP) and crop production (CP) and showed evidence of the conservation hypothesis. We found bidirectional causality between food production and agricultural land and between crop production and agricultural land, which shows evidence of the feedback hypothesis. Additionally, the empirical findings of a robustness check with fully modified ordinary least square (FMOLS) and dynamic ordinary least square (DOLS) techniques showed consistency with the investigations of ARDL estimation in the long run, ensuring the validity and strength of the primary outcomes. Overall, the present paper brings fresh knowledge about agricultural land use, and food and crop production to promote environmental sustainability. Full article