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Keywords = BnGPAT9

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18 pages, 5596 KB  
Article
Analysis of BnGPAT9 Gene Expression Patterns in Brassica napus and Its Impact on Seed Oil Content
by Man Xing, Bo Hong, Mengjie Lv, Xueyi Lan, Danhui Zhang, Chunlei Shu, Shucheng Qi, Zechuan Peng, Chunyun Guan, Xinghua Xiong and Luyao Huang
Agriculture 2024, 14(8), 1334; https://doi.org/10.3390/agriculture14081334 - 10 Aug 2024
Viewed by 1400
Abstract
Glycerol-3-phosphate acyltransferase (GPAT) genes encode enzymes involved in the biosynthesis of plant oils. Rapeseed has four BnGPAT9 genes, but the expression patterns and functions of these four homologous copies in rapeseed for seed oil accumulation are not well understood. In this [...] Read more.
Glycerol-3-phosphate acyltransferase (GPAT) genes encode enzymes involved in the biosynthesis of plant oils. Rapeseed has four BnGPAT9 genes, but the expression patterns and functions of these four homologous copies in rapeseed for seed oil accumulation are not well understood. In this study, we cloned the four BnGPAT9 genes and their promoters from Brassica napus and found significant differences in the expression of BnGPAT9 genes among different rapeseed varieties. We confirmed that BnGPAT9-A01/C01 are highly conserved in rapeseed, with high expression levels in various tissues, especially during the late stages of silique development and seed maturation. All four BnGPAT9 genes (BnGPAT9-A01/C01/A10/C09) can promote seed oil accumulation, but BnGPAT9-A01/C01 have a greater effect. Overexpression in Arabidopsis and rapeseed increased seed oil content and altered fatty acid composition, significantly increasing linolenic acid content. Transcriptome analysis revealed that BnGPAT9 genes promote the upregulation of genes related to oil synthesis, particularly those in the Plant–pathogen interaction, alpha-Linolenic acid metabolism, MAPK signaling pathway—plant, and Glutathione metabolism pathways. In summary, these results indicate that the four BnGPAT9 genes in rapeseed have different expression patterns and roles in regulating seed oil accumulation, with BnGPAT9-A01/C01 contributing the most to promoting oil accumulation. Full article
(This article belongs to the Special Issue Molecular Breeding and Genetic Improvement of Oilseed Crops)
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16 pages, 3910 KB  
Article
Overexpression of WAX INDUCER1/SHINE1 Gene Enhances Wax Accumulation under Osmotic Stress and Oil Synthesis in Brassica napus
by Ning Liu, Jie Chen, Tiehu Wang, Qing Li, Pengpeng Cui, Chengxi Jia and Yueyun Hong
Int. J. Mol. Sci. 2019, 20(18), 4435; https://doi.org/10.3390/ijms20184435 - 9 Sep 2019
Cited by 33 | Viewed by 3742
Abstract
WAX INDUCER1/SHINE1 (WIN1) belongs to the AP2/EREBP transcription factor family and plays an important role in wax and cutin accumulation in plants. Here we show that BnWIN1 from Brassica napus (Bn) has dual functions in wax accumulation and oil synthesis. Overexpression (OE) of [...] Read more.
WAX INDUCER1/SHINE1 (WIN1) belongs to the AP2/EREBP transcription factor family and plays an important role in wax and cutin accumulation in plants. Here we show that BnWIN1 from Brassica napus (Bn) has dual functions in wax accumulation and oil synthesis. Overexpression (OE) of BnWIN1 led to enhanced wax accumulation and promoted growth without adverse effects on oil synthesis under salt stress conditions. Lipid profiling revealed that BnWIN1-OE plants accumulated more waxes with elevated C29-alkanes, C31-alkanes, C28-alcohol, and C29-alcohol relative to wild type (WT) under salt stress. Moreover, overexpression of BnWIN1 also increased seed oil content under normal growth conditions. BnWIN1 directly bound to the promoter region of genes encoding biotin carboxyl carrier protein 1 (BCCP1), glycerol-3-phosphate acyltransferase 9 (GPAT9), lysophosphatidic acid acyltransferase 5 (LPAT5), and diacylglycerol acyltransferase 2 (DGAT2) involved in the lipid anabolic process. Overexpression of BnWIN1 resulted in upregulated expression of numerous genes involved in de novo fatty acid synthesis, wax accumulation, and oil production. The results suggest that BnWIN1 is a transcriptional activator to regulate the biosynthesis of both extracellular and intracellular lipids. Full article
(This article belongs to the Section Molecular Plant Sciences)
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