Search Results (658)

This paper introduces the GOLEM ontology, a novel framework designed to provide a structured and computationally tractable representation of narrative and fictional elements. Addressing limitations in existing ontologies regarding the integration of fictional entities and diverse narrative theories, our model extends CIDOC CRM and LRMoo and leverages DOLCE’s cognitive foundations to provide a flexible and interoperable framework. The ontology captures complexities of narrative structure, character dynamics, and fictional worlds while supporting provenance tracking and pluralistic interpretations. The modular structure facilitates alignment with various literary and narrative theories and integration of external resources. Future work will focus on expanding domain-specific extensions, validating the model through larger-scale case studies, and developing a reader response module to systematically model the reception of narratives. By fostering interoperability between literary theory, fan cultures, and computational analysis, this ontology lays a foundation for interoperable comparative research on narrative and fiction. Full article

The morbidity/mortality risks of cystic fibrosis (CF) with a delayed diagnosis have made newborn screening (NBS) attractive for the past 50 years. Initial efforts focused on meconium analyses, but these proved unsatisfactory. After dried blood spot specimens became valuable for NBS applied to other genetic disorders and immunoassay methods became routine, the discovery of immunoreactive trypsinogen (IRT) led to numerous CF NBS programs around the world. Excellent laboratorians led the way, but CF clinicians rightly questioned the benefit–risk relationship and unanswered questions about IRT. These issues were resolved by the combination of a positive randomized clinical trial and the discovery of the cystic fibrosis transmembrane conductance regulator gene (CFTR) and its principal pathogenic variant, F508del. Recommendations for universal screening and then the proliferation of IRT/DNA screening programs followed. But more knowledge has brought more complexity, including an enigmatic, distracting condition known as cystic fibrosis transmembrane conductance regulator-related metabolic syndrome (CRMS) or cystic fibrosis screen positive, inconclusive diagnosis (CFSPID). Recently, with the recognition that CF is not a “white person’s disease,” and that over 1000 CFTR pathogenic variants occur, attention has turned to achieving equity and timeliness for all babies. Continuous quality improvement has characterized the past decade, as greatly expanded CFTR panels in the DNA tier through next-generation sequencing offer promise and raise the prospect of a primary genetic screening test. Full article

This study evaluates the impact of an educational intervention aimed at improving the management of benign paroxysmal positional vertigo (BPPV) in a clinical setting. Interrupted Time Series (ITS) analysis was used to assess changes in the use of key diagnostic maneuvers—Dix–Hallpike and Supine Roll—as well as the treatment maneuver, Canalith Repositioning Maneuvers (CRM), and the use of CT scans. The intervention aimed to promote evidence-based practices and minimize unnecessary imaging. ITS models accounted for confounders such as history of BPPV and dizziness. The results showed significant improvements in the use of the Supine Roll and CRM maneuvers, with both immediate increases and sustained upward trends following the intervention. For the Dix–Hallpike maneuver, an initial increase was observed after the intervention; however, this effect was no longer statistically significant after adjusting for confounders, suggesting that the observed change may have been influenced by shifts in the patient population rather than the intervention itself. A positive long-term trend persisted. No significant reduction in CT scan usage was found. These findings demonstrate the intervention’s effectiveness in enhancing adherence to recommended diagnostic and treatment practices for BPPV. However, they also suggest that reducing unnecessary imaging may require additional strategies beyond provider education. Full article

Low-temperature oxidation of titanomagnetite in oceanic basalts distorts the primary thermoremanent magnetization (TRM) signal essential for reconstructing Earth’s magnetic field history, though the specific impact of magma intrusion-induced oxidation on paleointensity preservation remains poorly constrained. This investigation simulates such oxidation processes using a novel experimental design involving isothermal annealing (260 $°$C; 50 µT field; durations 12.5–1300 h) of Red Sea rift basalts (P72/4), employing the Thellier-Coe method to quantify how chemical remanent magnetization (CRM) overprinting affects TRM fidelity under controlled field orientations aligned either parallel or perpendicular to the initial TRM. Results demonstrate two-sloped Arai-Nagata diagrams with reliable TRM preservation below 360 $°$C but significant alteration artifacts above this threshold. Crucially, field orientation during oxidation critically influences accuracy: parallel configurations maintain fidelity (±3% deviation at $Z=0.48$), while perpendicular fields introduce systematic biases (38% overestimation at $Z=0.15$; 20% underestimation at $Z>0.48$), which is attributable to magnetostatic interactions in core-shell grain structures. These findings establish that paleointensity reliability in basalt prone to low-temperature oxidation depends fundamentally on the alignment between oxidation-era magnetic fields and primary TRM direction, necessitating stringent sample selection and directional constraints in marine paleomagnetic research to mitigate CRM-TRM interference. Full article

The Medina of Fez, a UNESCO World Heritage Site, is a cultural and historical treasure requiring innovative approaches for its preservation and promotion in the digital era. This paper presents MedinaOnto, an ontology dedicated to modeling the built cultural heritage of the Medina of Fez, developed in alignment with established standards like CIDOC-CRM, FOAF, and GEOSPARQL. The ontology aims to structure and interconnect knowledge about the Medina’s buildings while integrating social, geospatial, and historical dimensions. Based on the Core Data Standard and enriched with structured data from open sources such as Wikidata and DBpedia, the ontology aims to represent a wide range of heritage entities, including monuments, historic buildings, and culturally significant spaces. The modeling approach followed a structured ontology engineering methodology, combining domain analysis, concept extraction, the formulation of Competency Questions, and iterative refinement. The evaluation of the ontology was conducted through logical consistency tests, scenario-based validation, and SPARQL queries derived from Competency Questions. The results confirmed that MedinaOnto enables robust semantic reasoning and effective data querying. Designed as a reusable and scalable semantic framework, Medina Onto targets both public and heritage professionals, with a focus on valorization, mediation, and research. Full article

This study applies a culturally responsive lens to the analysis of middle school (i.e., grades for students aged 11–13) mathematics curriculum materials. Based on previous conceptual frameworks that describe Culturally Responsive Mathematics (CRM) as a multidimensional construct, we developed a tool, the CRM Materials Evidence Tool (CRM-MET), to indicate the extent of explicit guidance within written curriculum materials toward different dimensions of CRM. Six sets of middle school curriculum materials were analyzed using the CRM-MET, with results demonstrating distinct differences in how these materials attended to different dimensions of CRM. This analysis also indicated that there are notable gaps across all analyzed curricula, especially regarding more critical dimensions of CRM focused on power and participation. These results suggest that systems aimed at supporting teachers’ orientation toward and implementation of CRM can benefit from curriculum materials, but that the standardized nature of curriculum may also constrain the role of such materials in supporting CRM. We provide suggestions around how curriculum designers and school leaders might use curriculum strategically to support CRM given these findings, while recognizing policy constraints that may challenge such efforts. Full article

Cutaneous melanoma is an aggressive form of skin cancer and a leading cause of cancer-related mortality. In this sense, Raman Spectroscopy (RS) could represent a fast and effective method for melanoma-related diagnosis. We therefore introduced a new method based on RS to distinguish Compound Naevi (CN) from Primary Cutaneous Melanoma (PCM) from ex vivo solid biopsies. To this aim, integrating Confocal Raman Micro-Spectroscopy (CRM) with four Machine Learning (ML) algorithms: Linear Discriminant Analysis (LDA), Quadratic Discriminant Analysis (QDA), Support Vector Machine (SVM), and Random Forest Classifier (RFC). We focused our attention on the comparison between traditional pre-processing operations with Continuous Wavelet Transform (CWT). In particular, CWT led to the maximum classification accuracy, which was ∼89.0%, which highlighted the method as promising in view of future implementations in devices for everyday use. Full article

Background/Objectives: Group B Streptococcus (GBS) is a significant cause of perinatal infection in neonates and infants. Complications could include neonatal sepsis and meningitis, preterm birth, stillbirth, or death. Though no GBS vaccine is currently licensed, maternal immunization is expected to be a highly effective strategy to address invasive GBS disease—particularly in low- and middle-income countries (LMICs), where the disease burden is the greatest and access to existing interventions is limited. In this study, we present a novel hexavalent GBS vaccine candidate with a unique combination of serotypes (ST)—Ia, Ib, II, III, V, and VII—that could be an efficacious and cost-effective intervention, with the broadest coverage of 99% against circulating serotypes globally. Methods: The 6-valent conjugate vaccine candidate, GBS-06, is developed using a novel approach by linking the six polysaccharides (PS) to recombinant cross-reactive material 197 (rCRM197) carrier protein derivatized with a hydrazide-polyethylene glycol-hydrazide (HZ-PEG-HZ) linker. A repeat-dose GLP toxicology study with GBS-06 was conducted at the highest clinical dose of 20 µg in rabbits with saline as the placebo control. Results: The results reveal induction of robust anti-capsular polysaccharide-specific IgG responses against each of the six serotypes after each dose with the highest antibody GMCs at Day 49 following the third dose. Conclusions: Hence, this work is the first demonstration of strong immunogenicity achieved using a linker (HZ-PEG-HZ) for GBS glycoconjugate vaccine development. The positive data from the study have strong implications in the advancement of the candidate for evaluation in clinical trials and provide a licensure pathway for maternal immunization. Full article

Type 2 diabetes (T2D) is a chronic metabolic disorder marked by insulin resistance and impaired glucose metabolism, with skeletal muscle being a major site of systemic glucose disposal. This review examines the bidirectional relationship between T2D and sarcopenia, and synthesizes current evidence on how skeletal muscle deterioration and insulin resistance interact to disrupt glucose homeostasis. We summarize molecular mechanisms by which physical exercise enhances glucose uptake via insulin-dependent and insulin-independent pathways, and review the ergogenic and metabolic effects of creatine monohydrate (CrM). We also evaluate studies combining CrM supplementation with resistance or aerobic training and their effects on glycaemic control, muscle mass and function. Overall, combined exercise and creatine supplementation show potential to improve glucose regulation and attenuate muscle loss in older adults and people with T2D. Available data indicate that CrM is well tolerated in healthy and clinical populations when used at recommended doses, with no consistent evidence of adverse renal or hepatic effects. Further large randomized trials are needed to define optimal dosing, training modalities and long-term benefits for metabolic outcomes. Full article

Background: Bovine mastitis (BM) is a major disease affecting dairy herds (DHs), with Serratia marcescens (S. marcescens) being increasingly implicated as a causative agent. The growing concern over antimicrobial resistance (AMR) extends to BM-associated S. marcescens isolates, where resistance patterns are emerging. Methods: Here, four BM Gram-negative isolates were investigated: 1-DH1, 2-DH1, 3-DH2, and 4-DH3. Phenotypic characterization was performed using the Neg-Urine-Combo98 panel on a MicroScan WalkAway Plus system. Whole-genome sequencing (WGS) was performed to characterize and identify AMR and virulence factors (VF) genes and plasmids in isolates 1-DH1, 3-DH2, and 4-DH3, and phylogenomic analyses were conducted for a visual comparison of the genomes. Results: Phenotypically, isolates 1-DH1, 2-DH1, and 4-DH3 were identified as S. marcescens, and 3-DH2 as Serratia odorifera (confirmed as S. marcescens by WGS). A 28.00% (n = 25) prevalence of phenotypic AMR for isolates 1-DH1, 2-DH1, and 4-DH3 against Aug-E, AM, To, Cfx, Crm, Cl, and Fd was shown, and 24.00% (n = 25) for isolate 3-DH2 against Aug-E, AM, To, Crm, Cl, and Fd. The AMR genes AAC(6′)-Ic, aac(6′)-Ic_1, aac(6′)-Ial, H-NS, SRT-2, oqxB, oqxB_1, oqxB25, mexI, CRP, and blaSST-1, and flgH, fliP, fliM, and fliG VF genes were identified in the whole genome of the S. marcescens sequenced isolates 1-DH1, 2-DH1, and 4-DH3. In addition, a phylogenomic analysis of these three isolates revealed that WGS genomes are more closely related to S. marcescens prevenient from environmental sources. Conclusions: This study reports, for the first time, AMR resistance to tobramycin, cefuroxime, colistin, and nitrofurantoin in BM S. marcescens isolates. Genomic analysis revealed the presence of multiple AMR and VF genes, further highlighting the pathogenic potential of these isolates. Phylogenomic analysis revealed that the genome of the three BM S. marcescens isolates is more closely related to environmental S. marcescens strains. Full article

The energy transition is an issue of fundamental importance in the current global context, as an increasing number of countries are committed to searching for minerals and elements essential for the storage, distribution, and supply of energy derived from new renewable and sustainable sources. In some countries, these elements (such as boron, lithium, and strontium) are considered to be critical raw materials (CRMs) because of their limited occurrence within their own borders and are commonly found in minerals and geothermal–formation waters, especially in brackish to brine waters. In the Italian territory, CRM-rich waters have already been identified by previously published studies (i.e., with mean concentrations in the Salsomaggiore Terme of 390 mg/L of boron, 76 mg/L of lithium, and 414 mg/L of strontium); however, their extraction is hampered by several knowledge gaps. In particular, a comprehensive understanding of the origin, accumulation processes, and migration pathways of these CRM-rich waters is still lacking. These factors are closely linked to the geological framework and evolutionary history of each specific area. To address these gaps, we investigated the Salsomaggiore Structure that is located at the northwestern front of the Apennine in Italy by integrating geological data with hydrogeochemical results. We constructed new preliminary distribution maps of the most significant CRMs around the Salsomaggiore Structure, which can be used in the future for the National Mineral Exploration Program drawn up in accordance with the European Critical Raw Materials Act. These maps, combined with the interpretation of seismic reflection profiles calibrated with surface geology and wells, allowed us to establish a close relationship between water geochemistry/CRM contents and the geological evolution of the Salsomaggiore Structure. This structure can be considered representative of the frontal ranges of the Northwestern Apennine and other mountain chains associated with the foreland basin systems. Full article

Threat activation or deactivation in the brain–body is associated with learned nocebo or placebo somatic effects induced by fake invasive medical–surgical procedures. Some functional somatic disorders (FSDs) originate as acute nocebo somatic effects and can become 30–50% of chronic somatic presentations to primary care physicians. Patients with FSD overutilize medical–surgical services, despite the lack of identified pathophysiology, and are at risk for morbidity from unintentional iatrogenic injury. The Conditioned Response Model (CRM) of learning postulates three innate mechanisms, modulated by trait hypnotizability, which drive placebo and nocebo somatic effects and FSD. The High Risk Model of Threat Perception (HRMTP) postulates 10 psychosocial risk factors that modulate threat perception, driving placebo and nocebo somatic effectsandbiologically embedded FSD. Psychosocial factors and the trait of high and low hypnotizability modulate threat and are postulated to reduce heart rate variability(HRV), inducing autonomic nervous system(ANS)dysregulation. Reduced HRV was found in a large (N = 6,891) sample of patients with FSD. A total of 50% of patients with FSD with chronic pain (n = 224) without identified pathophysiology had a Paradoxical Increase in hand Temperature (PTI) during experimental threat induction. The HRMTP predicts that PTI associated with ANS dysregulation is associated with the risk factor Adverse Childhood Experiences (ACEs). This ACE prediction was independently confirmed. Learning predicts that threat activation by unconscious neutral stimuli (CS) can amplify nocebo and FSD and can negate placebo effects in clinician–patient relationships. Identifying psychosocial risk factors that modulate threat perception enables the diagnosis of FSD by inclusion and not simply by excluding pathophysiology. Full article

Background: This study explores the relationship among various supply chain management practices, including customer relationship management, lean practices, use of information technology, and quality of information sharing with operational performance in the readymade garments industry of Bangladesh. It also examines the mediating role of quality of information sharing in these relationships. Methods: Data were collected from 80 readymade garment companies across five different geographical locations, with companies of varying sizes (large, medium, and small), involving 365 respondents with a response rate of 65%. A self-administered questionnaire survey was conducted, and Partial Least Squares Structural Equation Modeling (PLS-SEM) was applied for the analysis. Results: The results indicate that all four practices significantly enhance operational performance, while customer relationship management and use of information technology also improve performance indirectly through quality of information sharing, unlike lean practices. Conclusions: The findings suggest that supply chain managers and stakeholders can improve operational performance by implementing supply chain management practices and understanding the complexities of their interrelationships. Full article

Climate change affects all human and ecological systems. The rapid climate impacts are increasingly evident on all economic activities, including tourism. Regarding the fact that “the window is closing”, climate resilience is urgently needed to protect tourism resources and maintain the quality of tourism offerings. Since the recent climate and tourism scientific literature emphasizes the necessity to mobilize existing knowledge, standardize practices, and explore appropriate tools related to tourism adaptation, we provided desk research and discussed the latest achievements of the Intergovernmental Panel on Climate Change’s (IPCC) and related knowledge platforms. According to the results of this review, it seems that the vast majority of the authors use vulnerability assessment (VA) to provide a solid basis for climate change adaptation (CCA) options applicable to tourism. Also, there is a lack of application of the latest IPCC recommendations founded in climate risk assessment (CRA). In the context of CRA, vulnerability was often assessed in a static way, with limited consideration of future hazards, probabilistic estimates, and the interactions between climatic and non-climatic drivers. Moreover, the methodologies applied to assess climate-related issues in tourism have been highly heterogeneous, hindering comparability and aggregation of results. Since risk is a useful conceptual framework for understanding tourism’s climate issues and modalities to reach its climate resilience, we discussed the significance of shifting the vulnerability concept towards a risk-centered framework. This review paper also provides a basis for a common understanding of CRA, a step-by-step approach to its assessment, and the explanation of CCA options to strengthen the tourism community, since a decisive decade of climate action is upon us. Full article

This study examines Chinese hotel employees’ understanding of customer relationship management (CRM) practices, with a focus on loyalty programme behaviour (LPB). Specifically, it investigates how three factors—loyalty programme (LP) awareness, LP knowledge, and LP concerns—shape employees’ intentions to implement loyalty programme practices (LPP) in the hospitality industry. A quantitative research design was adopted to test the proposed hypotheses. Data was collected through a self-administered survey of 893 Chinese hotel employees. To evaluate the proposed hypotheses, path analysis was performed using SPSS Statistics 24 and Mplus 7.4. The findings reveal that LP awareness and LP knowledge are both positively associated with employees’ intentions to implement loyalty programme practices, whereas LP concerns showed no significant effect. Moreover, employees’ intentions were positively linked to loyalty programme behaviour and served as a mediator between awareness, knowledge, and behavioural outcomes. Theoretical insights and practical applications are also addressed. Full article