Search Results (672)

In Haiti, uncontrolled urbanization is increasing pressure on agricultural landscapes, compromising both their ecological integrity and productivity. This study examines spatio-temporal land-use changes across three agricultural plains, Cul-de-Sac, Maribahoux, and Léogâne, between 1997 and 2024, using Landsat imagery and landscape metrics of composition (percentage of landscape, PLAND) and configuration (largest patch index, LPI). The findings reveal a rapid expansion of built-up areas, primarily at the expense of farmland. In the Cul-de-Sac plain, built-up areas and bare soil grew by 152%, from 41.26 km² to 104.11 km², while agricultural land became highly fragmented (LPI dropping from 94.51% to 57.63%). In Maribahoux, urbanization was more moderate, partly offset by a temporary rise in woody vegetation that peaked at 20.04% in 2022 before declining. The Léogâne plain experienced a 17.38 km² increase in built-up areas and bare soil, alongside a slight decrease in woody vegetation. Population density showed limited differences in Maribahoux and Léogâne, but marked disparities in Cul-de-Sac, where landscape transformation was more pronounced. These findings highlight increasing fragmentation of agricultural landscapes, threatening ecological connectivity and functionality, and stress the urgent need for land-use planning that curbs urban growth, protects farmland, and safeguards biodiversity. Full article

Human immunodeficiency virus (HIV), comprising two distinct types, HIV-1 and HIV-2, remains one of the most significant global health challenges, originating from multiple cross-species transmissions of simian immunodeficiency viruses (SIVs) in the early 20th century. This review traces the evolutionary trajectory of HIV from zoonotic spillover to its establishment as a global pandemic. HIV-1, the principal strain responsible for AIDS, emerged from SIVcpz in Central African chimpanzees, with phylogenetic evidence indicating initial human transmission between the 1920s and 1940s in present day Democratic Republic of Congo. The virus disseminated through colonial trade networks, reaching the Caribbean by the 1960s before establishing endemic transmission in North America and Europe. HIV’s extraordinary genetic diversity—driven by high mutation rates (~10⁻⁵ mutations per base per replication cycle) and frequent recombination events—has generated multiple groups, subtypes, and circulating recombinant forms (CRFs) with distinct epidemiological patterns. HIV-1 Group M, comprising subtypes A through L, accounts for over 95% of global infections, with subtype C predominating in sub-Saharan Africa and Asia, while subtype B dominates in Western Europe and North America. The extensive genetic heterogeneity of HIV significantly impacts diagnostic accuracy, antiretroviral therapy efficacy, and vaccine development, as subtypes exhibit differential biological properties, transmission efficiencies, and drug resistance profiles. Contemporary advances, including next-generation sequencing (NGS) for surveillance, broadly neutralizing antibodies for cross-subtype prevention and therapy, and long-acting antiretroviral formulations to improve adherence, have transformed HIV management and prevention strategies. NGS enables near real-time surveillance of drug resistance mutations and inference of transmission networks where it is available, although access and routine application remain uneven across regions. Broadly neutralizing antibodies demonstrate cross-subtype efficacy, while long-acting formulations have the potential to improve treatment adherence. This review synthesizes recent evidence and offers actionable recommendations to optimize clinical and public health responses—including the routine use of genotypic resistance testing where feasible, targeted use of phylogenetic analysis for outbreak investigation, and the development of region-specific diagnostic and treatment algorithms informed by local subtype prevalence. While the understanding of HIV’s evolutionary dynamics has substantially improved and remains essential, translating this knowledge into universally implemented intervention strategies remains a key challenge for achieving the UNAIDS 95-95-95 targets and the goal of ending AIDS as a public health threat by 2030. Full article

Spatial distribution of trace elements (TEs) in soils of the city of Dschang (Cameroon) was studied to identify their origin (geogenic vs. anthropogenic). The topsoil (at different depths) of 71 sites was analyzed using the rapid portable X-ray fluorescence analysis method. Soils from locations associated with metal-related activities exhibited the highest levels of contamination (average concentrations in mg kg⁻¹: As, 8.2; Cr, 213.7; Cu, 201.8; Pb, 97.4; Zn, 838.0), followed by household waste dumps and agricultural plots (levels close to those of cultivated low-lying areas). The observed decrease in TE concentrations with depth (notably for Zn) supports the hypothesis of a human origin (compared with soil-geochemical background of control sites). Geostatistical approach indicated an underestimation of health risks associated with the consumption of crops from several sites. Specifically, 87.32%, 49.30%, and 47.89% of the sites exceeded the Food Crops Reference Value (FCRV) for Cr, Zn, and Cu, respectively. Additionally, the number of contaminated sites for each TE varies depending on the method: Cu > Zn > Pb > Cr > As = Ni > Cd and Cr > Zn > Cu > Ni > Pb > As > Cd with the geostatistical and FCRV approach respectively. From the first step of the soil chemical quality investigation, our study highlights the need to use methods based on health risks, especially for sensitive uses of soils such as food production. Full article

Introduction: Trace metals can negatively impact biological functions and brain development. Cognitive and neurobehavioral disorders in children are poorly documented in Haut-Katanga Province, an area with significant and multiple exposures to trace metals that can lead to the exacerbation of cognitive and behavioral disorders. Objective: This study aimed to characterize the behavior of schoolchildren linked to their cognitive performance in urban and rural environments. Methods: A cross-sectional pilot study was conducted on 52 schoolchildren aged 6 to 11 (22 from rural areas presumed less exposed to metals and 30 from Lubumbashi, DRC). This study employed NEPSY-II tests, the Strengths and Difficulties Questionnaire (SDQ-Tutor), ENA 2020 software and trace metal spectrometry assays. Statistical tests were carried out with SPSS-20 and Stata-18. Results: Our findings revealed a correlation between children’s malnutrition and low mother’s education. The “total difficulties score” was more prevalent in rural areas (73%) compared to urban settings (37%) p < 0.05), in contrast to the “negative impact of difficulties” (59% versus 57%, p > 0.05). Urban children demonstrated superior cognitive performance, particularly in “facial affect recognition” (8 versus 4, p = 0.013) and “inhibitory control” (6.5 versus 3, p = 0.032). As-U(urine), Cd-B(blood), Hg-B, Mo-U, Ni-U, Pb-U, Pb-B and Sb-U were elevated compared to references. In general, urban areas had higher metal levels than rural areas. Blood and urine metals showed a complex and significant relationship with behavioral difficulties or cognitive performance. Conclusions: The observed behavioral issues, cognitive performance deficits and their association with nutritional deficiencies and trace metal exposure suggest a multifactorial neurodevelopmental origin. These findings highlight the need for further research in the region. Full article

The low fertility of plinthosols is a major constraint on agricultural production, largely due to the presence of plinthite, which restricts the availability of water and nutrients. This study aimed to simulate the growth and yield of grain maize on a loosened plinthosol amended with termite mound (from Macrotermes falciger) material in the Lubumbashi region. A 660-hectare perimeter was established, subdivided into ten maize blocks (B1–B10) and a control block (B0), which received the same management practices as the other blocks except for subsoiling and termite mound amendment. The APSIM model was used for simulations. The leaf area index (LAI) was estimated from Sentinel-2 imagery via Google Earth Engine, using the Simple Ratio (SR) spectral index, and integrated into APSIM alongside agro-environmental variables. Model performance was assessed using cross-validation (2/3 calibration, 1/3 validation) based on the coefficient of determination (R²), Nash–Sutcliffe efficiency (NSE), and root mean square error (RMSE). Results revealed a temporal LAI dynamic consistent with maize phenology. Simulated LAI matched observations closely (R² = 0.85 − 0.93; NSE = 0.50 − 0.77; RMSE = 0.29 − 0.40 m² m⁻²). Maize grain yield was also well predicted (R² = 0.91; NSE > 0.80; RMSE < 0.50 t ha⁻¹). Simulated yields reproduced the observed contrast between treated and control blocks: 10.4 t ha⁻¹ (B4, 2023–2024) versus 4.1 t ha⁻¹ (B0). These findings highlight the usefulness of combining remote sensing and biophysical modeling to optimize soil management and improve crop productivity under limiting conditions. Full article

Cabbage (Brassica oleracea Linnaeus) is an important vegetable crop for food security and income generation for farmers in the Democratic Republic of Congo (DRC). However, production is severely undermined by a complex of insect pests. This study investigates farmers’ knowledge, perception, and pest management practices in key cabbage-growing areas surrounding Goma city in Eastern DRC. A total of 430 farmers were interviewed using a structured survey administered via the KoboToolbox platform. The diamondback moth (Plutella xylostella Linnaeus, 1758) and the cabbage aphid (Brevicoryne brassicae Linnaeus, 1758) were identified as the main pests, with peak incidences reported during the dry mid-season. Pest damages are most frequently observed at the post-transplanting and heading stages of cabbage. Although chemical control was the dominant strategy (69.4%), concerns arise due to the widespread use of moderately to highly hazardous insecticides, including pyrethroid, organophosphorus, and avermectin-based formulations. The insufficient use of personal protective equipment (PPE) and limited training on safe pesticide handling remain further challenges. While indigenous practices, such as crop rotation, handpicking of insects, and the use of botanical extracts, are employed to a lesser extent, awareness and implementation of biological control are almost nonexistent. The findings underscore the need to promote integrated pest management (IPM) approaches based on agroecological principles, including the safe use of (bio-)pesticides, training programs, and stakeholder engagement to enhance sustainable cabbage production. Full article

Diamond traceability has been a major challenge for the gemological industry in recent decades. In this context, this paper presents new studies using UV-VIS-NIR spectroscopy to identify the traceability and geographical origin of diamonds. The aim of the work is to identify characteristic centers of fancy-color diamonds collected from Cullinan Mine, Democratic Republic of Congo (DRC), and the geographical regions with unknown origin. Depending on the origin of the diamonds, the UV-VIS-NIR spectra can be differentiated as follows: (i) the diamonds collected from Cullinan Mine show absorption bands assigned to N₁⁰, NV⁰, NV⁻, N₃V⁰, N₄V₂, and N₄V centers, which are accompanied by a vibronic structure localized between 415 and 394 nm (2.987–3.147 eV) and (ii) the diamonds from DRC show absorption bands attributed to N₁⁰, NV⁻, N₃V⁰, N₁⁺, and NVH centers. Using Raman spectroscopy, nitrogen concentration values of diamonds collected from the Cullinan mines and DRC between 41 and 185 ppm and 204–336 ppm, respectively, were reported. We prove that the simultaneous applicability of UV-VIS-NIR spectroscopy and Raman scattering as comparative tools for assessing diamond provenance can be a valuable strategy for an initial attribution of diamonds with unknown geographical origin, knowing the optical features of diamonds collected from Cullinan Mine and DRC. Full article

Yersinia pestis, the causative agent of plague, is arguably the most devastating pathogen in human history. Paleogenomic studies indicate its presence as early as the Neolithic era. It evolved from Yersinia pseudotuberculosis, with divergence estimates ranging from 1500 to 20,000 years ago, most often placed around 5000 years ago. Its natural reservoirs are wild mammals, particularly rodents, with fleas serving as vectors, while humans are incidental hosts. Over time, Y. pestis has acquired multiple virulence factors that disrupt immune responses and can lead to rapid, often fatal disease. Because the bacterium is maintained in wildlife cycles and can spill over to domestic animals, eradication is difficult, if not impossible. Nevertheless, mitigation is achievable using a One Health approach integrating human health, animal health, and the health of the environment. Neither vaccines nor monoclonal antibodies are currently licensed in most Western countries, thus, antibiotics remain the mainstay of therapy. Timely administration, ideally within 24 h of symptom onset, is critical, particularly in pneumonic forms. Phage therapy is under investigation as a potential treatment. Though often neglected in high-income settings, plague remains endemic in several regions, with the highest burden reported in Madagascar and the Democratic Republic of the Congo. Full article

Background: Women living with HIV (WLHIV) in low-income urban settings face multiple intersecting nutritional risks from food insecurity, poor dietary quality, and mental health problems. We evaluated the prevalence of household food insecurity and inadequate dietary diversity, examining their associations with depressive symptoms, antiretroviral therapy (ART)-related factors, and body mass index (BMI) among WLHIV attending routine ART clinics in Kinshasa, The Democratic Republic of Congo. This study addresses critical gaps in understanding the interplay between mental health and nutrition in the context of HIV care, with significant implications for improving health outcomes among vulnerable populations. Methods: In this clinic-based cross-sectional study (February–April 2024), we enrolled 571 women on ART in Masina 2, Kinshasa. Household food insecurity was measured using the Household Food Insecurity Access Scale (HFIAS), dietary diversity was assessed using the Minimum Dietary Diversity for Women (MDD_W; inadequate ≤ 5 food groups in 24 h), and probable depression was assessed using the Hopkins Symptom Checklist-10 (HSCL-10), which is a validated screening tool. We obtained baseline BMIs from clinic records at ART induction, which we measured again upon survey completion. We used analysis of covariance (ANCOVA) to model follow-up BMI, adjusting for baseline values, age, ART duration, self-reported adherence, household food insecurity, dietary diversity, and probable depression. Sensitivity analyses included change-score and mixed-effects models. Results: The prevalence of any household food insecurity was high (75%; 95% CI:71.5–78.6), with 57.6% (95% CI:53.5–61.6) of the participants experiencing inadequate dietary diversity (MDD_W < 5). Furthermore, forty-two per cent (95% CI:38.4–46.5) experienced depressive symptoms and sixty-eight percent (95% CI: 64.4–72.0) adhered to antiretroviral therapy (ART). The mean MDD_W was 4.3, with a low consumption rate of animal-source foods. Baseline BMI was associated with follow-up values (adjusted β_{unstandardized}, 0.48 kg/m² per 1 kg/m² baseline, 95% CI 0.38–0.59; p < 0.001). Probable depression was independently associated with a lower follow-up BMI (adjusted β_{unstandardized}, −0.99 kg/m²; 95% CI −1.72 to −0.26; p = 0.008). Time since ART initiation showed a slight positive association with BMI (adjusted β_{unstandardized}, 0.10 kg/m² per year). Self-reported ART adherence, household food insecurity, and dietary diversity were not independently associated with follow-up BMI in fully adjusted models. The interaction between age and probable depression did not suggest heterogeneity between age groups (p = 0.503). Conclusions: In our cohort, food insecurity and poor dietary diversity were widespread but did not significantly correlate with BMI, while probable depression, a potentially modifiable factor, was independently associated with lower BMI after accounting for baseline nutritional status. These findings highlight the need for HIV care programs integrating mental health screening and services with nutrition-sensitive interventions to support recovery and long-term health among WLHIV. Full article

A remarkable new pholcid spider species is described from the Democratic Republic of the Congo: Smeringopina polychila sp. nov. The male is distinguished by a unique and previously undocumented structure, here termed the “parachila”, which has not been observed in any other spider to date. The description is complemented by high-quality illustrations, including detailed drawings, photographs, micro-CT scans, and 3D reconstructions of the genitalia and the newly discovered male structure. Remarkable intraspecific variations, both somatic and genitalic, in males are also highlighted and discussed. A phylogenetic analysis based on the cytochrome c oxidase subunit I, 16S ribosomal RNA and histone H3 gene fragments is presented to tentatively place the new species into an existing phylogenetic framework. The results of the molecular analyses confirm that the new species belongs to the subfamily Smeringopinae and is nested within the genus Smeringopina Kraus, 1957. Full article

Engineering education in developing countries faces critical challenges that hinder progress toward achieving the United Nations Sustainable Development Goals (SDGs). In the Democratic Republic of Congo (DRC), students entering engineering programs often exhibit significant apprehension toward foundational sciences, creating barriers to developing the technical competencies required for sustainable development. This paper introduces the AI-Integrated Practical Learning in Engineering (AIPLE) Framework, an innovative pedagogical model that synergizes Problem-Based Learning (PBL), hands-on experimentation, and strategic Artificial Intelligence (AI) integration to transform engineering education for sustainability. The AIPLE framework employs a five-stage cyclical process designed to address student apprehension while fostering sustainable engineering mindsets essential for achieving SDGs 4 (Quality Education), 7 (Affordable and Clean Energy), 9 (Industry, Innovation and Infrastructure), and 11 (Sustainable Cities and Communities). This study, grounded in qualitative surveys of engineering instructors at Université Loyola du Congo (ULC), demonstrates how the framework addresses pedagogical limitations while building technical competency and sustainability consciousness. The research reveals that traditional didactic methods inadequately prepare students for complex sustainability challenges, while the AIPLE framework’s integration of AI-assisted learning, practical problem-solving, and sustainability-focused projects offers a scalable solution for engineering education transformation in resource-constrained environments. Our findings indicate strong instructor support for PBL methodologies and cautious optimism regarding AI integration, with emphasis on addressing infrastructure and ethical considerations. The AIPLE framework contributes to sustainable development by preparing engineers who are technically competent and committed to creating environmentally responsible, socially inclusive, and economically viable solutions for developing countries. Full article

Twenty natural products used in traditional medicine to treat dermatophytosis were evaluated for their efficacy against Trichophyton rubrum, the most frequent dermatophyte infecting humans. For this purpose, aqueous and methanolic extracts were prepared from ash, honey, and plant organs as pure (100%) or diluted (75%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.56%) preparations. The extracts were then evaluated by incorporating them into a Sabouraud medium and seeding them with T. rubrum as a fungal culture. The results identify fourteen extracts as being able to completely inhibit T. rubrum growth through either fungistatic or fungicidal activity. The five extracts with the highest efficacy to inhibit T. rubrum growth were further analyzed for their potential to alter in vitro reconstructed human epidermis (RHE). An aqueous extract from Allium sativum produced no tissue alteration in RHE, unlike the extracts from Conyza sumatrensis, Rumex abyssinicus, or Pentas longiflora. The data suggest that preparations used in traditional medicine by rural population in South-Kivu (DR-Congo) might represent valid alternatives to fight dermatophytosis. However, they also illustrate that several preparations remain inefficient and that others may be detrimental to the epidermis. This work reveals that traditional preparations, although affordable and easily available, require an evaluation of their efficacy and safety. Full article

Background/Objectives: This study examines the associations between household socioeconomic status (SES), child nutrition, and developmental status among children aged 24–59 months in the Mont Ngafula health zone in Kinshasa. The primary research question focuses on how SES and stunting affect developmental outcomes in early childhood. Methods: A cross-sectional analysis was conducted involving 348 children, assessing developmental outcomes using the Early Childhood Development Index (ECDI2030). Results: The study found that 70.4% of children were classified as on track, with ONTRACK prevalence increasing across SES tertiles. Children who attended preschool education had higher odds of being on track. The rich tertile had higher odds of being on track than those in the poor tertile, while the middle tertile showed a weaker association. Child age categories and stunting were inversely associated with being developmentally on track. The results are consistent with multiple imputation sensitivity analyses. Conclusions: The study concludes that preschool attendance and a higher household socioeconomic position are strongly associated with better early developmental outcomes, while an age of 48–59 months and stunting are associated with a markedly lower likelihood of being developmentally on track. Integrated policies that reduce household poverty, promote early education, and prevent/treat early faltering growth could improve early childhood developmental trajectories. Full article

Non-communicable diseases (NCDs) are the leading global cause of death, causing over 43 million deaths in 2021, including 18 million premature deaths, disproportionately affecting low- and middle-income countries. NCDs also incur significant economic losses, estimated at USD 7 trillion from 2011 to 2025, despite low prevention costs. This study evaluated body mapping indicators: body mass index (BMI), waist circumference, and waist-to-hip ratio—for predicting NCD risk, including hypertension, diabetes, and cardiovascular diseases, using data from a nationally representative survey in Ghana. The study sampled 5775 participants via multistage stratified sampling, ensuring proportional representation by region, urban/rural residency, age, and gender. Ethical approval and informed consent were obtained. Anthropometric and biochemical data, including height, weight, waist and hip circumferences, blood pressure, fasting glucose, and lipid profiles, were collected using standardized protocols. Data analysis was conducted with STATA 17.0, accounting for complex survey design. Significant sex-based differences were observed: men were taller and lighter, while women had higher BMI and waist/hip circumferences. NCD prevalence increased with age, peaking at 60–69 years, and was higher in females. Lower education and marital status (widowed, divorced, separated) correlated with higher NCD prevalence. Obesity and high waist circumference strongly predicted NCD risk, but individual anthropometric measures lacked screening accuracy. Integrated screening and tailored interventions are recommended for improved NCD detection and management in resource-limited settings. Full article

This study presents a comprehensive assessment of lighting conditions in the Intensive Care Units (ICUs) of two major hospitals in the Democratic Republic of Congo (DRC): Hospital du Cinquantenaire in Kinshasa and Jason Sendwe Hospital in Lubumbashi. A mixed-methods approach was employed, integrating continuous illuminance monitoring with structured staff surveys to evaluate visual comfort in accordance with the EN 12464-1 standard for indoor workplaces. Objective measurements revealed that more than 52.2% of the evaluated ICU workspaces failed to meet the recommended minimum illuminance level of 300 lux. Subjective responses from healthcare professionals indicated that poor lighting significantly reduced job satisfaction by 40%, lowered self-rated task performance by 30%, decreased visual comfort scores from 4.1 to 2.6 (on a 1–5 scale), and increased the prevalence of well-being symptoms (eye fatigue, headaches) by 25–35%. Frequent complaints included eye strain, glare, and discomfort with posture, with these issues often exacerbated during the rainy season due to reduced natural daylight. The study highlights critical deficiencies in current lighting infrastructure and emphasizes the need for urgent improvements in clinical environments. Moreover, inconsistent energy supply to these healthcare settings also impacts the assurance of visual comfort. To address these shortcomings, the study recommends transitioning to energy-efficient LED lighting, enhancing access to natural light, incorporating circadian rhythm-based lighting systems, enabling individual lighting control at workstations, and ensuring a consistent power supply via the integration of solar inverters to the grid supply. These interventions are essential not only for improving healthcare staff performance and safety but also for supporting better patient outcomes. The findings offer actionable insights for hospital administrators and policymakers in the DRC and similar low-resource settings seeking to enhance environmental quality in critical care facilities. Full article