Search Results (19)

The genotoxic drug doxorubicin (Dox) remains one of the most powerful chemotherapeutic options available for a wide range of cancers including breast, ovarian, and other cancers. However, emerging evidence links Dox treatment with chemotherapy-induced cognitive impairment, a condition that is popularly referred to as Dox-induced neurotoxicity or “chemobrain”, which limits the use of the drug. There are no specific treatments for Dox-induced neurotoxicity, only interventions to mitigate the neurotoxic effects of the drug. Accumulating evidence indicates that DNA damage, oxidative stress, dysregulation of autophagy and neurogenesis, inflammation, and apoptosis play central roles in Dox-induced neurotoxicity. Additionally, germline mutations in the tumour suppressor genes breast cancer susceptibility genes 1 and 2 (BRCA1 and BRCA2) increase the risk of breast, ovarian, and related cancers. BRCA1 and BRCA2 are distinct proteins that play crucial, unique roles in homologous recombination-mediated double-stranded break repair. Furthermore, BRCA1 and 2 mitigate oxidative stress in both neural cells and brain microvascular endothelial cells, which suggests that they have a critical role as regulators of pathways central to the development of Dox-induced neurotoxicity. Despite research on the effects of Dox on cognitive function, there is a gap in knowledge about the role of BRCA1 and BRCA2 in Dox-induced neurotoxicity. In this review, we discuss existing findings about the role of different mechanisms and the role of BRCA1 and BRCA2 in Dox-induced neurotoxicity, along with future perspectives. Full article

Cancer, a multifactorial disease characterized by uncontrolled cellular proliferation, remains a global health challenge with significant morbidity and mortality. Genomic and molecular aberrations, coupled with environmental factors, contribute to its heterogeneity and complexity. Chemotherapeutic agents like doxorubicin (Dox) have shown efficacy against various cancers but are hindered by dose-dependent cytotoxicity, particularly on vital organs like the heart and brain. Autophagy, a cellular process involved in self-degradation and recycling, emerges as a promising therapeutic target in cancer therapy and neurodegenerative diseases. Dysregulation of autophagy contributes to cancer progression and drug resistance, while its modulation holds the potential to enhance treatment outcomes and mitigate adverse effects. Additionally, emerging evidence suggests a potential link between autophagy, DNA damage, and caretaker breast cancer genes BRCA1/2, highlighting the interplay between DNA repair mechanisms and cellular homeostasis. This review explores the intricate relationship between cancer, Dox-induced cytotoxicity, autophagy modulation, and the potential implications of autophagy in DNA damage repair pathways, particularly in the context of BRCA1/2 mutations. Full article

Under different pathophysiological conditions, endothelial cells lose endothelial phenotype and gain mesenchymal cell-like phenotype via a process known as endothelial-to-mesenchymal transition (EndMT). At the molecular level, endothelial cells lose the expression of endothelial cell-specific markers such as CD31/platelet-endothelial cell adhesion molecule, von Willebrand factor, and vascular-endothelial cadherin and gain the expression of mesenchymal cell markers such as α-smooth muscle actin, N-cadherin, vimentin, fibroblast specific protein-1, and collagens. EndMT is induced by numerous different pathways triggered and modulated by multiple different and often redundant mechanisms in a context-dependent manner depending on the pathophysiological status of the cell. EndMT plays an essential role in embryonic development, particularly in atrioventricular valve development; however, EndMT is also implicated in the pathogenesis of several genetically determined and acquired diseases, including malignant, cardiovascular, inflammatory, and fibrotic disorders. Among cardiovascular diseases, aberrant EndMT is reported in atherosclerosis, pulmonary hypertension, valvular disease, fibroelastosis, and cardiac fibrosis. Accordingly, understanding the mechanisms behind the cause and/or effect of EndMT to eventually target EndMT appears to be a promising strategy for treating aberrant EndMT-associated diseases. However, this approach is limited by a lack of precise functional and molecular pathways, causes and/or effects, and a lack of robust animal models and human data about EndMT in different diseases. Here, we review different mechanisms in EndMT and the role of EndMT in various cardiovascular diseases. Full article

The Digital Product Passport (DPP) as a product-specific data set is a powerful tool that provides information on the origin or composition of products and increases transparency and traceability. This recycling case study accompanies the production of 2192 frisbees, which originated from collected beverage bottle caps. In total, 486.7 kg of feedstock was collected and transformed into 363.2 kg of final product with verified traceability through all process steps via a DPP, provided by the R-Cycle initiative and based on the GS1 standard. This demanded a generally agreed dataset, the availability of technical infrastructure, and additional effort in the processing steps to collect and process the data. R-Cycle offers a one-layer DPP where the data structure is lean and information is visible to everyone. This is beneficial to a variety of stakeholders in terms of transparency. However, it does not allow the sharing of sensitive information. On the one hand, the DPP has a high potential to be an enabler for customer engagement, origin verification, or as a starting point for more efficient and advanced recycling of plastics. On the other hand, the DPP involves a certain effort in data generation and handling, which must be justified by the benefits. For small, simple packaging items, the DPP may not be the perfect solution for all problems. However, with a broader societal mindset and legislative push, the DPP can become a widely used and trusted declaration tool. This can support the plastics industry in its journey towards a circular economy. Full article

Endothelial autophagy plays an important role in the regulation of endothelial function. The inhibition of endothelial autophagy is associated with the reduced expression of protein disulfide isomerase 4 (PDIA-4); however, its role in endothelial cells is not known. Here, we report that endothelial cell-specific loss of PDIA-4 leads to impaired autophagic flux accompanied by loss of endothelial function and apoptosis. Endothelial cell-specific loss of PDIA-4 also induced marked changes in endothelial cell architecture, accompanied by the loss of endothelial markers and the gain of mesenchymal markers consistent with endothelial-to-mesenchymal transition (EndMT). The loss of PDIA-4 activated TGFβ-signaling, and inhibition of TGFβ-signaling suppressed EndMT in PDIA-4-silenced endothelial cells in vitro. Our findings help elucidate the role of PDIA-4 in endothelial autophagy and endothelial function and provide a potential target to modulate endothelial function and/or limit autophagy and EndMT in (patho-)physiological conditions. Full article

Germline mutations in Breast cancer susceptibility genes 1 and 2 (BRCA1 and BRCA2) cause breast, ovarian, and other cancers, and the chemotherapeutic drug doxorubicin (Dox) is widely used to treat these cancers. However, Dox use is limited by the latent induction of severe cardiotoxicity known as Dox-induced cardiomyopathy, for which there are no specific treatments currently available. Dox is administered into the systemic circulation, where it readily translocates into sub-cellular compartments and disrupts the integrity of DNA. Accumulating evidence indicates that oxidative stress, DNA damage, inflammation, and apoptosis all play a central role in Dox-induced cardiomyopathy. The BRCA1 and BRCA2 proteins are distinct as they perform crucial yet separate roles in the homologous recombination repair of DNA double-strand breaks, thereby maintaining genomic integrity. Additionally, both BRCA1 and BRCA2 mitigate oxidative stress and apoptosis in both cardiomyocytes and endothelial cells. Accordingly, BRCA1 and BRCA2 are essential regulators of pathways that are central to the development of cardiomyopathy induced by Doxorubicin. Despite extensive investigations, there exists a gap in knowledge about the role of BRCA1 and BRCA2 in Doxorubicin-induced cardiomyopathy. Here, we review the previous findings and associations about the expected role and associated mechanisms of BRCA1 and 2 in Dox-induced cardiomyopathy and future perspectives. Full article

This study demonstrates an open-loop recycling process of a specific post-consumer plastic waste stream. The targeted input waste material was defined as high-density polyethylene beverage bottle caps. Two methods of waste collection, informal and formal, were employed. Thereafter, materials were hand-sorted, shredded, regranulated, and then injection-molded into a flying disc (i.e., frisbee) as a pilot product. To observe the potential changes in the material throughout the entire recycling process, eight different test methods including melt mass-flow rate (MFR), differential scanning calorimetry (DSC), and mechanical tests were carried out on the various material states. The study showed that the informal collection led to a relatively higher purity in the input stream, which also appeared to have a 23% lower MFR value compared to that of the formally collected materials. The DSC measurements revealed a cross-contamination by polypropylene, which clearly affected the properties of all investigated materials. The cross-contamination led to a slightly higher tensile modulus in the recyclate, while the Charpy notched impact strength declined after processing by approximately 15% and 8% compared to those of the informal and formal input materials, respectively. All materials and the processing data were documented and stored online as a practical implementation of a digital product passport as a potential digital traceability tool. Furthermore, the suitability of the resulting recyclate to be used in transport packaging applications was also investigated. It was found that a direct replacement of virgin materials for this specific application is not possible without proper material modification. Full article

This study examined the effect of small-sided football games (SSFG) and small-sided Ultimate Frisbee games (SSUFG) on working memory, response times, and feeling mood of boys with overweight. Twenty-eight boys (age 12.23 ± 1.58 years) participated in two trials during physical education lessons (20-min SSFG and 20-min SSUFG) in a counterbalanced, randomized crossover design. The response times and feeling mood were measured for all participants post-exercise through the Sternberg paradigm and feeling scale. For the response times, the paired samples t-test revealed a significantly better reaction time after SSUFG at the “One item level” of the Sternberg paradigm (p = 0.014, Hedges’ g = 0.27, small effect) and (p = 0.010, Hedges’ g = 0.74, medium effect), at “Three item level” (p = 0.000106, Hedges’ g = 1.88, very large effect). The SSFG also showed vigor at the “Five item level” (p = 0.047, Hedges’ g = 0.61, medium effect). For the feeling mood, the feeling score was significantly higher after the SSUFG session than the SSFG session and the increase in feeling scores observed after switching from SSFG to SSUFG was significantly different (p < 0.001) from the decrease observed in feeling scores after switching from SSUFG to SSFG. Therefore, the results of the study allow the teacher to introduce new sports and reflect on the motor tasks he or she proposes for boys with overweight during physical education classes. Full article

The aim of this study was to determine the effects of different teaching methodologies (comprehensive vs. technical), developed during a unit of ultimate frisbee, on the physical demands, technical–tactical variables, game performance and physical fitness of secondary school children. Forty-seven students were divided into two groups: Comprehensive Teaching Group (CTG; n = 25, Age: 14.5 ± 0.5 years) and Technical Teaching Group (TTG; n = 22, Age: 14.9 ± 0.8 years). The teaching models (CTG vs. TTG) were composed of 8 sessions of 55 min. Physical fitness was developed with the Alpha-Fitness method, while physical variables were analysed using GPS; the technical and tactical variables performed during the matches were recorded using Full-HD video cameras. The results showed that the CTG students had greater mean speed (p < 0.001), mean heart rate (p = 0.006) and covered more total distance/time (p < 0.001) than the TTG students during sessions. After the intervention, the CTG students showed greater values in good passes (p = 0.001), good catches (p = 0.001), good decisions (p = 0.002) and game performance (p < 0.001), while the TTG students only showed higher values in game performance (p = 0.039). In conclusion, the comprehensive teaching model is shown to be an effective pedagogical option to learn ultimate frisbee because the students achieved a higher overall development in the physical demands expressed in the sessions and in the management of the technical–tactical actions in the offensive and defensive phases of the ultimate frisbee game. Full article

In Ultimate Frisbee, players are responsible for administering and reinforcing adherence to the rules of the game. As a self-refereed sport, UF relies upon the Spirit of the Game (SOTG). This study aimed to profile the experience, to ascertain the training history of the sport, the participation and goal orientations of international Ultimate Frisbee players in the European Beach Ultimate Championship, and to evaluate the contribution of these variables in a discriminant function to classify players according to SOTG level and competitive level. The sample consisted of 160 players [females 33.8% (29.3 ± 7.2 years); males 66.2% (37.6 ± 9.7 years)] who competed in the European Beach Ultimate Championship, 2019. Factorial ANOVA was used to test the effect of sex, competitive level and SOTG level (measured by the sum of the scores obtained in five questions addressing the following domains: (1) Knowledge and use of the rules; (2) Fouls and body contact; (3) Fair-mindedness; (4) Positive attitude and self-control; (5) Communication. The results revealed that sex was not a consistent source of SOTG level variation among players. In each competitive level, those with high SOTG levels obtained lower European Beach Ultimate Championship classification (t = 5.73 to 6.55, p < 0.001, d = 1.28 to 2.06), higher SOTG classification (t = −13.21 to −7.04, p < 0.001, d = 1.28 to 2.85), and better evaluation for fouls and body contact (t = 2.76 to 9.23, p < 0.05, d = 0.86 to 1.99) and for positive attitude (t = 5.88 to 7.43, p < 0.001, d = 2.06 to 2.57), than regular SOTG level players. Players of different competitive levels demonstrated similar SOTG results. These findings provide important information to coaches, physical education teachers and sport consultants. Full article

The aim of the present study was to investigate the effect of secondary school students’ fitness profile on physical and technical–tactical performance in simulated competition conditions of ultimate frisbee when there is no previous experience in the practice of the sport. Forty-three secondary school students participated in this research. The students were divided into two groups according to their results in the Assessing Levels of Physical Activity and fitness test battery (ALPHA fitness test): poor physical fitness (PPF) (N = 24; age: 14.9 ± 0.8 years; height: 166.1 ± 10.9 cm; body mass: 62.2 ± 11.0 kg; ALPHA fitness score: 2.7 ± 0.7 points) and good physical fitness (GPF) (N = 19; age: 14.5 ± 0.6 years; height: 165.9 ± 5.8 cm; body mass: 58.9 ± 7.5 kg; ALPHA fitness score: 4.4 ± 0.3 points). Physical variables during the ultimate frisbee match were assessed using Global Positioning System technology. The matches were video-recorded, and individual technical actions were noted afterwards. The GPF group showed higher values for running (p = 0.039), high-speed running (p = 0.015), sprinting (p = 0.022) and total distance covered (p = 0.025) than the PPF group. In addition, more passes (p = 0.019), offensive decision making (p = 0.009) and player participation (p = 0.046) were recorded in the GPF group than the PPF group. Correlational analysis revealed a positive relationship (p < 0.05) between individual participation and the meters covered for jogging, running, running at high speed and sprinting during the game. In conclusion, although the students were novices in ultimate frisbee, the high physical fitness level had a positive effect on the game performance. Physical education teachers should consider this information when introducing new sports into their physical education classes. Full article

In human activity recognition (HAR), activities are automatically recognized and classified from a continuous stream of input sensor data. Although the scientific community has developed multiple approaches for various sports in recent years, marginal sports are rarely considered. These approaches cannot directly be applied to marginal sports, where available data are sparse and costly to acquire. Thus, we recorded and annotated inertial measurement unit (IMU) data containing different types of Ultimate Frisbee throws to investigate whether Convolutional Neural Networks (CNNs) and transfer learning can solve this. The relevant actions were automatically detected and were classified using a CNN. The proposed pipeline reaches an accuracy of 66.6%, distinguishing between nine different fine-grained classes. For the classification of the three basic throwing techniques, we achieve an accuracy of 89.9%. Furthermore, the results were compared to a transfer learning-based approach using a beach volleyball dataset as the source. Even if transfer learning could not improve the classification accuracy, the training time was significantly reduced. Finally, the effect of transfer learning on a reduced dataset, i.e., without data augmentations, is analyzed. While having the same number of training subjects, using the pre-trained weights improves the generalization capabilities of the network, i.e., increasing the accuracy and F1 score. This shows that transfer learning can be beneficial, especially when dealing with small datasets, as in marginal sports, and therefore, can improve the tracking of marginal sports. Full article

High quality Physical Education should instigate and support all learners to develop into a lifelong participant in a way which upkeeps their own health, fitness, and well-being. There are, however, an ever-increasing number of children who drop out of participating in physical activities at the earliest opportunity, leading to an increase in sedentary lifestyles and a rise in childhood obesity. It is evidence such as this which indicates Physical Education, specifically in England, is not currently appropriate for all and requires change. To attempt to make the subject a more positive experience for all and to inspire lifelong involvement, varying the curriculum and including alternative activities for pupils might tap into useful wider cultures. This paper discusses the emergence of alternative sports, the challenges and synergies of implementation, and focuses on what could work and why. Full article

Ultimate Frisbee (UF) is a non-contact, challenging, and self-promoted team sport. Its characteristics, such as the game environment and rules, appear to influence the on-the-pitch behaviour of players. This article examines the content of qualitative studies to determine if and how the unique characteristics of UF may be related to nine sociological themes, that may be identified during gameplay. These themes include the following: (a) competition and performance; (b) enjoyment; (c) communication; (d) cooperation/friendship; (e) behaviors/welfare; (f) teamwork/social skills; (g) environment/lifestyle; (h) rules/self-refereeing and (i) spirit of the Game (SOTG). The review was conducted according to PRISMA guidelines. A comprehensive search protocol was used to identify, screen, and select published research articles under a Qualitative Systematic Review (QSR). The search was occurred from 1 June to 30 December 2020 with no limitations regarding the year of publication. Original English-language papers that contained relevant data regarding sociological themes and UF were selected. As a result, nine papers were qualified to be included in the final version of QSR. The files analyzed were structured with MAXQDA. A total of 521 references were identified and selected for analysis. After the Screening (n = 301) and Eligibility (n = 71) phases, a total of 30 potential papers were selected and classified. Nine studies were included in the final analysis. The three most cited sociological themes in these studies were: communication, teamwork/social skills, and spirit of the game. Research suggests that UF involves patterns of interaction related to communication and the spirit of the game that encourage active lifestyles. Finally, we point out that UF is an appropriate sport to include in physical education classes in which the creation of positive relationships between students is the desired outcome. This topic should be explored further through interventional studies done in different contexts although the evidence suggests that UF offers players unique opportunities to experience a combination of physical activity and enjoyment. Full article

Small-sided games (SSGs) are common drills used in various team sports, but the exercise intensity in ultimate Frisbee SSG has not yet been investigated. To clarify the physical, physiological, and technical demands of ultimate Frisbee SSG, we investigated the influence of pitch size on exercise intensity during SSG. Nine male college ultimate Frisbee players played (3 vs. 3) SSG on small (SSG_S: 30 × 15 m) and large (SSG_L: 40 × 20 m) pitches; both SSGs comprised of four 4 min periods, interspersed by 5 min of passive recovery. Players’ mean heart rate (170 ± 8 and 171 ± 7 bpm), peak heart rate (184 ± 7 and 184 ± 5 bpm), and blood lactate concentration (11.3 ± 4.7 and 11.8 ± 4.6 mmol/L) were similar in SSG_S and SSG_L, respectively. The total distance covered (1984 ± 166 m and 1702 ± 80 m) and the distance covered during quick (860 ± 112 m and 696 ± 69 m) and high-intensity running (439 ± 95 and 255 ± 44) in SSG_L were significantly longer than those in SSG_S (p < 0.05). Conversely, the number of accelerations (45 ± 3 and 41 ± 3) and decelerations (44 ± 3 and 40 ± 4), catching errors (2 ± 1 and 1 ± 1), and turnovers (8 ± 2 and 6 ± 2) in SSG_S were significantly greater than those in SSG_L (p < 0.05). This study suggests that ultimate Frisbee SSG provides high-intensity training, which stimulates the glycolytic pathway. Furthermore, manipulating SSG pitch size effectively modulates the physical demands of SSG. Full article