Search Results (751)

Methane fluxes in disturbed peatlands can exhibit significant heterogeneity with regard to land cover composition on abandoned peat extraction areas. The temporal and spatial variability of CH₄ fluxes is considered in this paper in the context of a detailed vegetation classification on a typical milled peatland in the Baltic region of Russia (Kaliningrad oblast, Rossyanka Carbon Supersite). The findings are derived from the analysis of 12,000 air samples obtained by the opaque emission chamber method at 10 peatland sites with different environmental characteristics during regular measurement campaigns of 2022–2024. The emission data have been mapped using a multilevel B-spline interpolation procedure. The mean cumulative methane flux was found to be 18.7–28.8 kg ha⁻¹yr⁻¹, which is close to the IPCC conventional value of 32.9 kg ha⁻¹yr⁻¹ estimated for boreal and temperate zones. However, environmental distinctions across the peatland sites result in considerable emission heterogeneity ranging from −0.02 to 11.5 kg ha⁻¹month⁻¹. Temperature is considered a principal factor responsible for the baseline CH₄ emission level in seasonal scale, while hydrology defines emission rate during the warm period of the year and in the inter-annual scales. Five peatland site types have been defined according to a level of methane emissions. Full article

Postnatal stem cells are crucial for tissue homeostasis and repair and are regulated by specialized microenvironmental microterritories known as “stem cell niches”. Proposed by R. Schofield in 1978 for hematopoietic stem cells, niches maintain self-renewal, guide differentiation and maturation, and can even revert progenitor cells to an undifferentiated state. Niches respond to injury, oxygen levels, mechanical cues, and signaling molecules. While the niche concept has advanced regenerative medicine, bioengineering, and 3D bioprinting, further progress is hindered by inconsistent interpretations of its core principles. To address this, we proposed a consensus-building initiative among experts in regenerative medicine and bioengineering. We have developed a questionnaire covering the niche topography, hierarchy, dimension, geometry, composition, regulatory mechanisms, and specifically the mesenchymal stem cell niches. This pilot survey, being conducted under the auspices of the National Society for Regenerative Medicine in the Russian Federation, aims to establish a standardized framework on the eve of the 50th anniversary of Schofield’s hypothesis. The resulting consensus will guide future research and innovation in this pivotal field. Full article

Judging a person’s appearance as beautiful or ugly is not a factual description but an aesthetic act. Kant believed that the human figure is the ideal of beauty, and at the same time, beauty can positively symbolize morality. However, regarding the correlation between aesthetics and morality, the Chapter “De Chong Fu 德充符” in the Zhuangzi offers us a strikingly insightful perspective that is transcendent and religious. The characters depicted in “De Chong Fu 德充符” have incomplete physical forms and ugly appearances, which seemingly do not qualify as symbols of morality in Kant’s sense. Yet, their behavior and demeanor precisely reflect their detachment from external physical forms and their inner virtue being full. This inner virtue embodies a religious ethic that transcends individual and subjective perspectives. This paper posits that there is a deeper and more thought-provoking connection between virtue and physical form in Zhuangzi’s philosophy. The aim of this paper is to explore the issue of self-transcendence in Zhuangzi’s philosophy from the perspective of religious ethics, using the relationship between the body, beauty, and virtue in Lao–Zhuang philosophy as a guiding thread. This paper will be divided into three main parts: the first part introduces the topic through the symbolic relationship between beauty and morality in Kant; the second part discusses the transcendence of aesthetics and physical form in Daoist philosophy and provides a section-by-section analysis of Chapter “De Chong Fu 德充符” of the Zhuangzi; and the third part summarizes the relationship between virtue and form in “De Chong Fu 德充符”. The paper argues that the transcendence of the physical form by virtue, as reflected in Chapter “De Chong Fu 德充符”, is an embodiment of religious ethics. In Lao–Zhuang philosophy, due to their deep understanding of the nature of beauty and the nature of the body, there is no necessary positive connection between beauty and form. Virtue does not necessarily need to rely on beauty as a symbol to be realized, and there can still be a positive connection between a defective physical form and virtue. In conclusions, the paper proposes the modern inspiration of self-transcendence reflected in Zhuangzi’s “De Chong Fu 德充符”. Full article

This essay develops a distinct moral argument for the reasonableness of believing in God (conceived as a perfectly good creator) inspired by the pragmatic argument for “the religious hypothesis” advanced by William James in “The Will to Believe.” It also contextualizes the argument relative to familiar moral arguments, notably those of C.S. Lewis and Kant. Briefly, the argument developed here holds that when facing more than one coherent picture of reality, each of which could be true based on the arguments and evidence but only one of which fulfills the hope that in a fundamental way reality is on the side of moral goodness (what I call “the ethico-religious hope”), a reasonable person could opt to believe in the hope’s fulfillment and live accordingly. Following James’ approach, however, this argument does not imply that others who do not adopt such a picture are necessarily irrational or less rational. Full article

The article aspires to delineate Hegel’s appropriation of Aristotle’s concept of soul in post-Kantian European Philosophy. It showcases the way that Hegel fuses central aspects of Aristotle’s theory and Kant’s account of inner purposiveness in order to deliver a hierarchical account of vegetative-, animal-, and human organisms. The article is divided in six parts. The first part offers an introduction to the subject matter. The second part delivers an overall account of the way that Hegel reconstructs Aristotle’s general theory of the soul and fuses it with Kant’s theory of inner purposiveness. The third part highlights Hegel’s distinctive argumentative strategy for delivering a unified, atemporal account of the connection between the various natural realms, both inorganic and organic. Parts four, five and six proceed to his theory of vegetative-, animal- and human life respectively, while showcasing the underlying logic and the upshot of Hegel’s developmental account of these three kinds of life qua soul. Full article

Kidney transplantation is the treatment of choice for patients with end-stage kidney disease. Despite significant advances in graft survival, rejection continues to pose a major clinical challenge. Conventional monitoring tools, such as serum creatinine, donor-specific antibodies, and proteinuria, lack sensitivity and specificity for early detection of graft injury. Moreover, while biopsy remains the current gold standard for diagnosing rejection, it is prone to confounders, invasive, and associated with procedural risks. However, non-invasive novel biomarkers have emerged as promising alternatives for earlier rejection detection and improved immunosuppression management. This review focuses on the leading candidate biomarkers currently under clinical investigation, with an emphasis on their diagnostic performance, prognostic value, and potential to support personalised immunosuppressive strategies in kidney transplantation. Full article

Engineering of Cupriavidus necator could enable the production of various polyhydroxyalkanoates (PHAs); particularly, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HH)), a biopolymer with enhanced mechanical and thermal properties compared to poly(3-hydroxybutyrate) (PHB), can be efficiently produced from vegetable oils. However, challenges remain in the recovery process, particularly in removing residual oil and minimizing degradation of the polymer structure during extraction steps. This study investigated the effects of ethanol-based defatting on the recovery and polymeric properties of P(3HB-co-3HH). The proposed method involves the addition of ethanol to the cell broth to effectively remove residual oil. Ethanol improved the separation of microbial cells from the broth, thereby streamlining the downstream recovery process. Using ethanol in the washing step increased the recovery yield and purity to 95.7% and 83.4%, respectively (compared to 87.4% and 76.2% for distilled water washing), representing improvements of 8.3% and 7.2%. Ethanol washing also resulted in a 19% higher molecular weight compared to water washing, indicating reduced polymer degradation. In terms of physical properties, the elongation at break showed a significant difference: 241.9 ± 27.0% with ethanol washing compared to water (177.7 ± 10.3%), indicating ethanol washing retains flexibility. Overall, an ethanol washing step for defatting could simplify the recovery steps, increase yield and purity, and retain mechanical properties, especially for P(3HB-co-3HH) from oils. Full article

Mango is highly sensitive to heat stress, which directly affects the yield and quality. The extreme heat waves of 2024, with temperatures reaching 41–47 °C over 25 days, caused significant impacts on sensitive cultivars. The impact of heat waves on ten commercial cultivars from subtropical regions viz.,‘Dashehari’, ‘Langra’, ‘Chausa’, ‘Bombay Green’, ‘Himsagar’, ‘Amrapali’, ‘Mallika’, ‘Sharda Bhog’, ‘Kesar’, and ‘Rataul’, and thirteen selected elite hybrids H-4208, H-3680, H-4505, H-3833, H-4504, H-1739, H-3623, H-1084, H-4264, HS-01, H-949, H-4065, and H-2805, is reported. The predominant effects that were observed include the following: burning symptoms or blackened tips, surrounded by a yellow halo, with premature ripening in affected parts and, in severe cases, tissue mummification. Among commercial cultivars, viz., ‘Amrapali’ (25%), ‘Mallika’ (30%), ‘Langra’ (30%), ‘Dashehari’ (50%), and ‘Himsagar’ and ‘Bombay Green’ had severe impacts, with ~80% of fruits being affected, followed by ‘Sharda Bhog’. In contrast, mid-maturing cultivars like ‘Kesar’, ‘Rataul’, and late-maturing elite hybrids, which were immature during the stress period, showed no symptoms, indicating they are tolerant. Biochemical analyses revealed significantly elevated total soluble solids (TSS > 25 °B) in affected areas of sensitive genotypes compared to non-affected tissues and tolerant genotypes. Aroma profiling indicated variations in compounds such as caryophyllene and humulene between affected and unaffected parts. The study envisages that the phenological maturity scales are indicators for the selection of climate-resilient mango varieties/hybrids and shows potential for future breeding programs. Full article

Epilepsy is one of the most prevalent chronic medical conditions that really can affect individuals at any age. A broader study of the pathogenesis of the epileptic condition will probably serve as the cornerstone for the development of new antiepileptic remedies that aim to treat epilepsy symptomatically as well as prevent the epileptogenesis process or regulate its progression. Cellular changes in the brain include oxidative stress, neuroinflammation, inflammatory cell invasion, angiogenesis, and extracellular matrix associated changes. The extensive molecular profiling of epileptogenic tissue has revealed details on the molecular pathways that might start and sustain cellular changes. In healthy brains, epilepsy develops because of vascular disruptions, such as blood–brain barrier permeability and pathologic angiogenesis. Key inflammatory mediators are elevated during epileptic seizures, increasing the risk of recurrent seizures and resulting in secondary brain injury. Prostaglandins and cytokines are well-known inflammatory mediators in the brain and, after seizures, their production is increased. These inflammatory mediators may serve as therapeutic targets in the clinical research of novel antiepileptic medications. The functions of inflammatory mediators in epileptogenesis are covered in this review. Oxidative stress also plays a significant role in the pathogenesis of various neurological disorders, specifically epilepsy. Antioxidant therapy seems to be crucial for treating epileptic patients, as it prevents neuronal death by scavenging excess free radicals formed during the epileptic condition. The significance of antioxidants in mitochondrial dysfunction prevention and the relationship between oxidative stress and inflammation in epileptic patients are the major sections covered in this review. Full article

In recent decades, heavy metal pollution has become a significant environmental stress factor. Plants are characterized by high biochemical plasticity and can adjust their metabolism to ensure survival under a changing environment. Here we report, to our knowledge, the first gas chromatography-mass spectrometry (GC-MS)-based metabolomics study of Zn-induced stress responses in Amaranthus caudatus plants. The study was performed with root and leaf aqueous methanolic extracts after their lyophilization and sequential derivatization with methoxylamine hydrochloride and N-methyl-N-(trimethylsilyl)trifluoroacetamide. In total, 419 derivatives were detected in the samples, and 144 of them could be putatively annotated. The metabolic shifts in seven-week-old A. caudatus plants in response to a seven-day treatment with 300 µmol/L ZnSO₄·7H₂O in nutrient solution were organ-specific and more pronounced in roots. Most of the responsive metabolites were up-regulated and dominated by sugars and sugar acids. The revealed effects could be attributed to the involvement of these metabolites in osmotic regulation, antioxidant protection and Zn²⁺ complexation. A 59-fold up-regulation of gluconic acid in roots distinctly indicated enhanced glucose oxidation due to oxidative stress upon the Zn treatment. Gluconic acid might be further employed in Zn²⁺ complexation. Pronounced Zn-induced up-regulation of salicylic acid in roots and shoots suggested a key role of this hormone in stress signaling and activation of Zn stress tolerance mechanisms. Overall, our study provides the first insight into the general trends of Zn-induced biochemical rearrangements and main adaptive metabolic shifts in A. caudatus. Full article

Focal and segmental glomerulosclerosis (FSGS) describes a histological pattern of injury seen by light microscopy in kidney biopsy specimens and is the end result of various injuries to the podocyte. Our understanding of this disease entity has evolved greatly since it was first described, with particular focus on changes in the classification of FSGS as a disease entity and expansion in our understanding of the underlying pathophysiology. The incidence and prevalence of FSGS and FSGS-associated end-stage kidney disease (ESKD) have increased globally, particularly in the United States; it is now the most common primary glomerular disorder in those with ESKD. APOL-1 is likely responsible for this epidemiological trend in kidney disease in the US and is an important focus of clinical trials and potential targeted therapies. Currently, the goal of treatment in FSGS is to achieve remission of proteinuria and to prevent progression to ESKD. Remission is achieved by using immunosuppressive therapies in primary FSGS, but treatment in secondary and genetic FSGS is largely supportive. Recurrent FSGS (rFSGS) post-transplantation remains a significant clinical challenge to nephrologists; current monitoring and treatment strategies are based on retrospective meta-analysis and observational studies with no clear consensus as to the optimum approach. Emerging therapies are focused on developing more targeted interventions in genetic and secondary FSGS. This review article aims to comprehensively explore this multifaceted disease entity. Full article

Background: Cardiovascular diseases (CVD) are recognized as a leading cause of morbidity and mortality in the population worldwide. A healthy cardiovascular system enables adaptation to trauma and physical stress. This study targets the reciprocal relation between CVD and postoperative complications after trauma surgery. Methods: In 2014, a randomized and standardized acquisition of study patients was initiated at a Level I trauma center in Germany. The burden of CVDs and the location, type, and severity of injuries were categorized. Clavien–Dindo classification was used to record postoperative complications. Results: The study analyzed a cohort of 1262 patients, representing a diverse range of orthopedic treatment disciplines within the hospital. It highlighted that patients with lower leg fractures experienced significantly higher complication rates, particularly linked to heart valve diseases and chronic venous insufficiency. Age and sex were not found to have a significant impact. Multivariate analysis identified additional key influencing factors for the development of complications, including the number of CVDs, nutritional status, smoking habits, and mobility levels. Conclusions: CVDs play a pivotal role in elevating complication rates post-trauma-surgery. Trauma-related extremity conditions are notably more severe when accompanied by CVD. A personalized approach that accounts for cardiovascular risk factors could significantly improve treatment outcomes in the future. Full article