Search Results (4,457)

In the context of autonomous vehicles, proper lane merging is critical as it can reduce the traffic bottleneck and lead to safer road transportation. To obtain a collision-free and efficient lane merging, advanced control algorithms need to be designed to smoothly coordinate multiple vehicles to form a platoon. Model predictive control (MPC) is such a controller capable of forecasting future states of multiple vehicles by optimizing their control inputs while satisfying the constraints. Prior MPC-based studies mostly utilized offline planning with a precomputed lookup table of feasible maneuvers to model lane merging. Although these model designs reduce the online computational load, they lack flexibility, as they rely on predefined scenarios and cannot easily adapt to dynamic or unpredictable situations. In this study, we present a centralized MPC framework capable of online trajectory tracking under dynamic constraints and disturbances, for collision-free operation in tightly spaced multi-vehicle platoons. To evaluate the flexibility of our online algorithm, we examine the role of prediction horizon—the time window over which future states are forecasted—and platoon size in determining both the feasibility and efficiency of merging maneuvers. Our results reveal that there exists an optimal prediction horizon at which braking and acceleration can be minimized, thereby reducing energy consumption by 35–40%. Additionally, we observe that increasing the prediction horizon beyond the minimum required for feasibility can alter the vehicle sequence in the platoon. Capturing the changes in vehicle sequence (e.g., who leads or yields) when prediction horizon varies, is a consequence of online trajectory optimization. This vehicle sequence change cannot be captured by offline planning that relies on precomputed look-up table maneuvers. We also found that as the number of vehicles increases, the minimum feasible prediction horizon increases significantly. Full article

Fungi are a prolific source of diverse bioactive metabolites, yet many remain unexplored. Among these, depsidones are a rare class of compounds with significant biological potential, but they are seldom reported in mushrooms. This study investigated the medicinal fungus Ganoderma lucidum, known for its extensive therapeutic use in traditional medicine. Fruiting bodies were extracted using petroleum ether, ethyl acetate, n-butanol, and methanol. Extracts were screened phytochemically and assessed for total phenolic content and antioxidant activity using the DPPH assay. Ethyl acetate extract exhibited the highest phenolic yield and antioxidant potential and was subsequently evaluated for cytotoxicity against HepG2, HCT116, MCF7, and A549 cancer cell lines. It showed notable anticancer activity with minimal toxicity to normal Vero cells. UHPLC/Q-TOF-MS/MS analysis of G. lucidum ethyl acetate extract tentatively identified nine minor depsidones including mollicellin G, simplicildone I, mollicellin B, talaromyone B, simplicildone A, purpactin C, emeguisin B, mollicellin E, and simplicildone D on the basis of high-resolution negative-mode detection and characteristic MS/MS fragmentation patterns. Molecular docking revealed strong binding affinities between these compounds and cancer-related targets (AKT1, CDK2, ERK1, TNFα), with simplicildone D and mollicellin G demonstrating particularly high interactions. These findings provide mechanistic insights into the observed bioactivity and highlight G. lucidum as a promising source of therapeutic depsidones for future anticancer drug development. Full article

The present study showed that it is possible add value to eucalyptus harvest waste, obtained in large quantities, from the cellulose industries, without known economic use, for the production of an activated biochar. The biochar, produced from the impregnation of eucalyptus harvest waste with H₃PO₄, and subsequently pyrolyzed at 600 °C for 1 h, was successfully used as a bioadsorbent in the removal of paracetamol, an emerging pollutant present in wastewater. The biochar showed a high specific surface area with micro- and mesopores and functionalized surface. The optimal conditions for the removal of paracetamol achieve an efficiency around 88–93%. The Langmuir and the pseudo-first-order models best fit the experimental data, with a maximum adsorption capacity of approximately 27.8 mg g⁻¹, at 25 °C. The thermodynamic showed that adsorption occurred spontaneously, endothermally and randomly at the solid–liquid interface. In addition, the bioadsorbent showed excellent reusability and no significant difference in adsorption capacity was observed in more complex aqueous matrices. Thus, the activated biochar produced in this study proved to be an efficient, low-cost and environmentally friendly bioadsorbent, capable of removing paracetamol from contaminated water, with great potential for use in water treatment plants, on a large scale and economically, contributing to the improvement of water quality and minimizing residual biomass in the environment. Full article

Background/Objectives: During and after electric–acoustic stimulation (EAS) surgery (as well as regular cochlear implant surgery), the oral and/or intravenous administration of steroids is recommended to prevent acute inflammatory reactions and subsequent fibrosis. However, the effect does not last long. Therefore, with the hope of providing a sustained effect, a new dexamethasone (DEX)-eluting electrode (FLEX28 DEX) has recently been developed. Methods: A case study was performed at Shinshu University in February 2024 in which a DEX-eluting electrode array was utilized for a patient presenting with high-frequency hearing loss with a defined etiology (hearing loss due to a mitochondrial m.1555A > G variant). Results: Residual hearing was well preserved after EAS surgery, and post-operative impedance field telemetry was maintained at a very low level in contrast with a historical/retrospective control group (FLEX28 electrodes without DEX); therefore, it is expected that post-operative fibrosis will be minimized. Further, it was shown that the DEX-eluting electrode can also be applied to EAS. Conclusions: The DEX-eluting electrode was useful in maintaining post-operative impedance at a very low level, indicating that post-operative fibrosis could be minimized even after EAS surgery. Full article

This study investigates the spatial distribution characteristics and influencing factors of soil organic carbon (SOC) in the Tamarix chinensis forest ecosystem in Changyi National Marine Ecological Special Reserve, China. Five sampling routes and 32 sampling points were established; 293 soil samples were collected every 10 cm from the surface downwards. GIS spatial analysis techniques were employed to analyze the overall, horizontal, and vertical distribution characteristics of SOC within the 0–100 cm depth range. The results show that SOC content in the reserve ranges from 1.0 to 10.0 gC/kg, with an average of 2.5–8.2 gC/kg. High-SOC zones are in the southwest, where human disturbance is minimal and vegetation is dense, whereas low-SOC areas are in the west, and the north suffers from frequent tides and salinization. Horizontally, the surface SOC (0–10 cm) increased from 2.30 gC/kg in the east to 9.15 gC/kg in the western tidal flat. Vertically, six profile types were identified; the fluctuating type dominated (74.07%). Eight ecological zones were delineated based on land cover and function: the Tamarix core area exhibited surface aggregation with a depth-wise decline; the ecological restoration zone showed a mid-depth peak; and the moisture-proof dam zone displayed a “shallow-rise–deep-drop” pattern. Storm surges, moisture-proof dams, ecological zoning, and restoration projects were key drivers of SOC distribution. Full article

Coral reefs, essential yet increasingly threatened marine ecosystems, rely on coral–microbiome symbioses for resilience against environmental stressors. This study investigates host-specific influences on bacterial communities in four Pocillopora species (Pocillopora eydouxi, Pocillopora meandrina, Pocillopora verrucosa, and Pocillopora woodjonesi) from the South China Sea. Using Illumina-based 16S rRNA gene sequencing, we analyzed microbiome structures, identified core taxa, and predicted metabolic functions. Results revealed that bacterial composition differed significantly among coral hosts, despite their shared habitat. P. eydouxi exhibited the highest bacterial richness and Shannon index, contrasting with minimal values in P. woodjonesi. A conserved core microbiome of 32 ASVs (1.1% of total ASVs), dominated by Gammaproteobacteria, was shared across all coral species. Host-specific enrichment of probiotic bacteria (Psychrobacter in P. eydouxi and Exiguobacterium in P. meandrina) and pathogenic taxa (e.g., Acinetobacter) was also observed. Functional prediction indicated conserved metabolic pathways across species, particularly amino acid and carbohydrate metabolism. These findings highlight host phylogeny as one of the primary determinants of microbiome assembly, providing critical insights into coral conservation strategies. Full article

The phenological characteristics of the spring phytoplankton bloom in the mid- and high-latitude oceans, including its initiation, duration, and intensity, can be assessed using various diagnostic methods. However, there is currently a lack of systematic comparisons among these different methods. To elucidate the differences in spring bloom characteristics derived from different approaches and to identify suitable methods for shelf seas, this study comprehensively compares and evaluates the multiple methods for characterizing the spring bloom in the central Yellow Sea, based on satellite-derived chlorophyll-a (Chl-a) data from 2003 to 2020. The methods examined include concentration threshold (CT), cumulative concentration threshold (CCT), rate of change (RoC), and curve-fitting methods for determining bloom initiation; threshold and symmetric methods for estimating duration; and peak, mean, integral, and relative intensity index methods for assessing intensity. The results show that the bloom initiation determined by the CT method occurs earliest (average: Day of Year (DOY) 64), whereas the RoC method identifies a notably later initiation (average: DOY 100), approximately 40 days later. The CCT method yields an intermediate bloom initiation (average: DOY 70), with minimal interannual variability. Notably, curve-fitting methods often produce outliers (e.g., DOY 1) due to the fluctuations in Chl-a time series during winter. The threshold method yields a shorter bloom duration (average: 70 days), while the symmetric method results in a duration of more than 10 days longer. The four intensity assessment methods indicate that bloom intensity initially increased and subsequently decreased from 2003 to 2020, but the peak year varies depending on the method used. Overall, the CCT, symmetric, and relative index methods are more suitable for the Yellow Sea, as their computational results exhibit fewer outliers and relatively low standard deviations. The interannual variations in spring bloom characteristics assessed by different methods display distinct patterns and weak correlations, indicating that methodological choices can lead to divergent interpretations of spring bloom dynamics. Therefore, it is essential to carefully select methods based on research objectives and dataset characteristics. Full article

Background/Objectives: Hepatitis B virus (HBV) infection poses a major global health challenge, with current therapies like nucleos(t)ide analogs and pegylated interferon alpha offering limited functional cure rates due to persistent HBsAg-driven immune tolerance. This study aimed to develop a targeted immunoadsorption system using a high-affinity humanized anti-HBsAg monoclonal antibody for efficient HBsAg and viral particle clearance, providing a novel approach to overcome therapeutic bottlenecks in chronic hepatitis B (CHB). Methods: A murine anti-HBsAg monoclonal antibody was humanized via complementarity-determining region grafting, resulting in HmAb-12 (equilibrium dissociation constant, K_D = 0.36 nM). A stable Chinese Hamster Ovary K1 (CHO-K1) cell line was established for high-yield expression (fed-batch yield: 8.31 g/L). The antibody was covalently coupled to agarose microspheres (coupling efficiency > 95%) to prepare the immunoadsorbent. Efficacy was evaluated through in vitro dynamic circulation assays with artificial sera and preclinical trials using an integrated blood purification system in two CHB participants. Clearance rates for HBsAg and HBV DNA were quantified, with safety assessed via blood component monitoring. Results: In vitro, a single treatment cycle achieved HBsAg clearance rates of 70.14% (high antigen load, >10⁵ IU/mL) and 92.10% (low antigen load, ~3000 IU/mL). Preclinically, one treatment session resulted in acute HBsAg reductions of 78.30% and 74.31% in participants with high and moderate antigen loads, respectively, alongside HBV DNA decreases of 65.66% and 73.55%. Minimal fluctuations in total protein and albumin levels (<15%) confirmed favorable safety profiles, with no serious adverse events observed. Conclusions: Preliminary findings from this study indicate that the HBsAg-specific immunoadsorption system can achieve efficient HBV antigen clearance with an initial favorable safety profile in a small cohort. These results support its further investigation as a potential therapeutic strategy for functional cure in CHB. Future work will focus on validating these findings in larger studies and exploring the system’s combinatory potential with existing blood purification platforms. Full article

Primary or recurrent infection of cytomegalovirus (CMV) in pregnant women may cause transplacental transmission to fetuses. We aimed to investigate the rate of transplacental CMV transmission in women with positive anti-CMV IgG and negative anti-CMV IgM and its impact on newborns. Pregnant women with positive anti-CMV IgG and negative anti-CMV IgM during the first or second trimester who delivered by Cesarean section were included. Amniotic fluid collected during the Cesarean section was tested for CMV DNA with quantitative real-time polymerase chain reaction. CMV IgG and IgM were measured with enzyme-linked immunosorbent assay. A total of 695 pregnant women were enrolled between April 2019 and February 2023. Of them, 567 (81.6%) were single pregnancies and 128 (18.4%) were twin pregnancies, and 594 (85.5%) were full-term pregnancies and 101 (14.5%) were premature pregnancies. Of the 823 newborns, 7 (0.9%) were CMV DNA positive in amniotic fluid, demonstrating the transplacental CMV transmission. One of these seven neonates was diagnosed with intrauterine growth restriction at gestation week 25⁺¹ and at birth at a gestational age of 30⁺² weeks. However, all seven children had normal hearing, vision, and neurodevelopment at the age of 18–56 months. Transplacental CMV transmission may occur in offspring of pregnant women with positive anti-CMV IgG and negative anti-CMV IgM, but the long-term sequelae appear to be minimal. Full article

Background and Objectives: Automated insulin delivery (AID) systems represent a major advancement in type 1 diabetes (T1D) management, particularly in pediatric populations. However, real-world evidence comparing their effectiveness to conventional multiple daily injection (MDI) therapy in youth remains limited. This study aimed to evaluate the impact of transitioning from MDI therapy to AID systems on glycemic control in children and adolescents with T1D, and to explore potential differences based on baseline HbA1c levels and device type. Materials and Methods: In this single-center, retrospective observational study, 76 children and adolescents with T1D were evaluated before and after switching from MDI to either the Medtronic MiniMed™ 780G or Tandem t:slim X2™ Control-IQ system. Glycemic control was assessed using continuous glucose monitoring (CGM)-derived metrics at three time points: the last 15 days of MDI therapy (T0), 15 days after (T1), and 6 months after (T2) AID initiation. Statistical comparisons were conducted across time points and between subgroups stratified by baseline HbA1c and AID system. Results: Significant improvements in glycemic control were observed as early as 15 days after AID initiation, with sustained benefits at 6 months. Time in range (TIR) increased from 62.0% at baseline to 76.7% at 15 days and 75.8% at 6 months, and time in tight range (TITR) from 39.8% to 53.9% at T1 and 52.1% at T2 (both p < 0.001). Improvements were more pronounced in participants with higher baseline HbA1c (+16.9% for TITR and +22.3% for TIR). No significant differences in glycemic outcomes were observed between device groups, although algorithm-driven differences in insulin delivery patterns were noted. Total daily insulin dose and BMI increased significantly over time (p < 0.001 and p = 0.008, respectively). Conclusions: AID therapy leads to rapid and sustained improvements in glycemic control among youth with T1D, particularly in those with suboptimal baseline control. These benefits highlight the clinical value of AID systems, while also emphasizing the need for monitoring potential metabolic impacts. Full article

Among other uses, DC-DC converters are employed in the auxiliary power modules (APMs) of electric vehicles (EVs), connecting the high-voltage traction battery to the low-voltage auxiliary system (AS). Traditionally, the APM is an isolated two-port, two-level (2L) DC-DC converter, and the auxiliary loads are fed at a fixed voltage level, e.g., 12 V in passenger cars. Dual-active-bridge (DAB) converters are commonly used for this application, as they provide galvanic isolation, high power density and efficiency, and bidirectional power flow capability. However, the auxiliary loads do not present a uniform optimum supply voltage, hindering overall efficiency. Thus, a more flexible approach, providing multiple supply voltages, would be more suitable for this application. Multiport DC-DC converters capable of feeding auxiliary loads at different voltage levels are a promising alternative. Multilevel neutral-point-clamped (NPC) DAB converters offer several advantages compared to conventional two-level (2L) ones, such as greater efficiency, reduced voltage stress, and enhanced scalability. The series connection of the NPC DC-link capacitors enables a multiport configuration without additional conversion stages. Moreover, the modular nature of the ML NPC DAB converter enables scalability while using semiconductors with the same voltage rating and without requiring additional passive components, thereby enhancing the converter’s power density and efficiency. This paper proposes a modulation strategy and decoupled closed-loop control strategy for the generalized multiport 2L-NL NPC DAB converter interfacing the EV traction battery with the AS, and its performance is validated through hardware-in-the-loop testing and simulations. The proposed modulation strategy minimizes conduction losses in the converter, and the control strategy effectively regulates the LV battery modules’ states of charge (SoC) by varying the required SoC and the power sunk by the LV loads, with the system stabilizing in less than 0.5 s in both scenarios. Full article

In cemented endoprosthetics, closed prepacked mixing systems represent the most advanced generation of cementing technology. (1) Background: The purpose of the present study is to evaluate four approved prepacked systems—Palacos^® R+G pro, SmartMix™ Cemvac GHV, Optipac^® Refobacin and Cemex^® System Genta—with a focus on practical handling and intraoperative hygiene. (2) Method: The systems were evaluated according to established standard test methods for bone cements (ISO 5833), including dough time, setting time, additional mechanical tests and the level of system closure. (3) Results: The results show that all systems are safe to use and meet the general requirements, but there are relevant differences in terms of intraoperative hygiene. The Palacos R+G pro system shows significantly shorter doughing and setting times, which helps to minimize wound exposure during surgery and thus significantly reduces the overall operating time and the risk of bacterial contamination. Two of the systems cannot be classified as completely closed “pre-packaged systems.” In two cases, the system must be temporarily opened before mixing to insert the mixing element, which may result in a temporary but clinically relevant impairment of sterility and a corresponding potential risk of contamination. (4) Conclusion: From a hygienic point of view, systems that remain completely closed throughout the entire preparation process can offer advantages in terms of infection prevention. This was the case for all systems tested. Short handling times, reduced exposure of the surgical site and a shorter overall duration of the procedure could further improve intraoperative safety and reduce the risk of contamination. In terms of intraoperative hygiene, the Palacos R+G pro system achieved the best results compared to the three other systems tested due to its rapid readiness for use and comparatively short setting time (according to ISO 5833). Cemex System Genta performed worst in this respect due to its late doughing time and setting time. Full article

This work studies yellow pea flour (YPF) fermentation with kefir (1:1.5 mass ratio, incubated 30 h at 25 °C) for gluten-free breadmaking. Three samples were evaluated: untreated YPF, YPF mixed with kefir (UF), and fermented YPF (FF). Structural changes were minimal, but fermentation improved the flour functionality. Bulk density (g/mL) decreased from 0.54 ± 0.02 in YPF and 0.47 ± 0.01 in UF to 0.43 ± 0.01 in FF, while the water absorption capacity (g/g) increased from 1.20 ± 0.01 in YPF and 1.50 ± 0.05 in UF to 1.92 ± 0.02 in FF. YPF showed the lowest oil absorption capacity (0.90 ± 0.02 g/g), while higher values were obtained for FF and UF (averaging 1.54 g/g). The yellowness index showed a clear tendency: higher in UF (34.9 ± 0.2), intermediate in FF (32.869 ± 0.008), and lower in YPF (22.4 ± 0.1). In gluten-free bread, baking loss did not show significant differences between FF-B and UF-B (averaging 15.65%) but they were significantly lower than that of YPF-B (18.5 ± 0.5%). The highest specific volume (mL/g) was observed in FF-B (1.96 ± 0.02), followed by UF-B (1.33 ± 0.02) and YPF-B (1.08 ± 0.02). Significantly reduced “pea” sensory attributes were perceived in FF-B, while acidity perception increased. Hardness was similar among breads, although chewiness was higher in FF-B. These results suggest that kefir fermentation enhances YPF functionality in gluten-free breadmaking. Full article

Hyaluronic acid and carboxymethyl cellulose are eco-friendly polysaccharides known for their excellent moisture retention properties, making them suitable components of agricultural fertilizers. On the other hand, humic acids exhibit surface-active properties, suggesting their potential to replace synthetic surfactants in agricultural applications. Naturally, the interaction between polysaccharides and humic substances influences their colloidal and chemical behavior. The mutual interactions between humic acids and these polysaccharides were examined at immiscible liquid interfaces and on plant leaf surfaces using radiotracer analysis and tensiometry (pendant drop and sessile drop methods). The results indicate that humic acids achieve optimal adsorption at a hyaluronic acid concentration of 30 g/L, regardless of molecular weight. In contrast, carboxymethyl cellulose reduces the surface activity of humic acids. Additionally, a combined solution of humic acids and hyaluronic acid improves the wetting efficiency of wheat leaves compared to individual solutions. However, humic acids showed minimal impact on the absorption or systemic distribution of hyaluronic acid within the plant. Full article

Gravity beneficiation is a key operation in mineral processing and waste recycling, enabling the production of concentrates with required quality. Among gravity separators, pulsating jigs remain widely applied due to their robustness and adaptability. This study evaluates the KOMAG laboratory jig for upgrading diverse feedstocks: hard coal with variable ash content, gravel aggregates with organic impurities, post-mining waste, and battery scrap. Tests were performed on a two-chamber jig with an air-pulsation system and advanced control. The results confirmed the feasibility of obtaining coal concentrates with 8%–10% ash at 59%–71% yield, complete removal of organic contaminants from aggregates with minimal losses, and recovery of combustible fractions from post-mining waste with favourable separation parameters (d₅₀ = 1.569 g/cm³, imperfection = 0.191). Beneficiation of shredded battery scrap achieved 74%–88% plastic removal and over 99% metallic recovery. Industrial implementations of KOMAG pulsating jigs validated these findings, showing high efficiency in coal, aggregate, and waste processing. This study demonstrates the versatility of pulsating jigging and its relevance in sustainable resource management, confirming that laboratory results can be effectively scaled to industrial practice. Full article