Search Results (700)

Portugal contributed to the global diffusion of sweetpotato (Ipomoea batatas L. Lam.). Although it is of minor importance on the Portuguese mainland, it is one of the most common crops in the Azores and Madeira archipelagos and is highly relevant in the Portuguese ex-colonies Mozambique and Timor-Leste. We analyzed the genetic diversity and population structure of sweetpotato from these five geographic provenances using twelve nuclear simple sequence repeat (SSR) markers. We studied 45 accessions, 15 of which were collected from farmers’ fields in these five regions and 30 of which are held at “Banco de Germoplasma de Moçambique”. The SSR markers showed a high level of polymorphism and a high number of alleles per locus. Population structure analyses using Bayesian clustering (STRUCTURE) grouped accessions from farmers’ fields into two groups and divided samples of “Banco de Germoplasma de Moçambique” into three groups. A principal coordinate analysis (PCoA), based on the Bruvo distance, supported the population structure analysis. Concerning the genebank accessions, the two analyses indicated three clusters, all of them containing Mozambican landraces. From our results, it may be concluded that sweetpotato populations from the three countries do not share a common genetic background, despite the shared history of the countries. Full article

Access to safe water and sanitation remains a pressing challenge in Sub-Saharan Africa. Rapid urbanisation, fragile governance, and increasing climate hazards continue to undermine the sustainability of WASH (Water, Sanitation and Hygiene) services. This study examines whether Mozambique’s normative and institutional framework effectively supports sustainable urban WASH service delivery in Beira, the country’s second-largest city. Combining a critical policy review with six semi-structured interviews involving institutional actors and community leaders, the research employs a qualitative, phenomenological design to explore the interaction between national frameworks and local practices. Findings reveal five interrelated dimensions shaping sustainability: governance coordination, infrastructure robustness and maintenance, community participation, climate resilience, and financial viability. Although post-disaster investments and recent policy reforms have led to improvements, significant challenges persist. These include overlapping institutional mandates, underdeveloped preventive maintenance systems, limited recognition and support for community-led initiatives, fragmented climate adaptation efforts, and strong dependence on external funding. The study also reveals how historical legacies, particularly colonial-era governance structures, continue to shape water and sanitation delivery. By integrating policy analysis with local perspectives, the paper contributes to debates on WASH sustainability in African cities, particularly in climate-vulnerable secondary urban centres. It highlights the need for systemic reforms that clarify institutional roles, institutionalise maintenance practices, formalise community engagement, embed nature-based adaptation strategies, and strengthen financial transparency. These changes are essential if Beira, and similar cities across sub-Saharan Africa, are to achieve Sustainable Development Goal 6 under mounting climate pressure. Full article

Greenhouse gas (GHG) fluxes from forests, including carbon dioxide (CO₂), methane (CH₄), and nitrous oxide (N₂O), are regulated by complex interactions of abiotic and biotic factors. A better understanding of these interactions involving GHGs can help manage forests and enhance their sequestration potential. This review examines how soil properties (moisture, temperature, and pH) and tree species-specific traits (litter quality, carbon storage, and microbial regulation) interactively control GHG dynamics in temperate forest soils, moving beyond a single-factor perspective. This literature review confirms that temperate forest soils are CH₄ sinks and sources of CO₂ and N₂O; however, flux direction and magnitude differ across spatial and temporal scales. CH₄ fluxes show high spatial variability and are sensitive to biogeochemical conditions. While soil temperature and moisture are well studied, their combined effects with site-specific variables such as substrate availability, soil texture, and canopy structure remain underexplored. Tree litter plays a dual role: chemically influencing microbial physiological/functional traits through priming, thereby affecting CO₂ and N₂O, and physically limiting CH₄ diffusion. These mechanisms collectively determine whether soils act as GHG sources or sinks, and future research should account for how litter priming may override their carbon sink function while integrating site-specific factors to improve GHG predictions and forest management. Full article

Estimated climate change scenarios demand robust coffee cultivars tolerant to supra-optimal temperatures, water deficit, diseases, and other stresses. Wild Coffea species represent important genetic resources for resilience. The study of variations in morphological structures associated with transpiration control, such as stomata, represents an important approach for identifying genotypes with greater stress tolerance. This study evaluated stomatal density and morphology in 48 wild accessions, 24 of Coffea racemosa and 24 of C. zanguebariae, from provinces of Mozambique. Leaf samples provided microscopic images to assess stomatal traits: density (SD), polar diameter (PD), equatorial diameter (ED), stomatal functionality (SF), and leaf dry mass. Principal components were analyzed for all 48 accessions and separately by species. Mean distribution independence was tested with the Mann–Whitney test (p < 0.05). Results revealed inter- and intraspecific variation. The ability to distinguish accessions varies with the set of traits and species. A significant negative correlation between ED and SF was shared by both species, suggesting a conserved functional pattern. This study discusses the differences in stomatal traits between wild and commercial coffee species and aspects related to possible alterations of stomatal structures during their adaptation to climate change. Additionally, it points to accessions with potential use in genetic breeding programs to increase stomatal function and the possible adaptation of new cultivars. Full article

Background: Professional football requires more attention in planning work regimens that balance players’ sports performance optimization and reduce their injury probability. Machine learning applied to sports science has focused on predicting these events and identifying their risk factors. Our study aims to (i) analyze the differences between injury incidence during training and matches and (ii) build and classify different predictive models of risk based on players’ internal and external loads across four sports seasons. Methods: This investigation involved 96 male football players (26.2 ± 4.2 years; 181.1 ± 6.1 cm; 74.5 ± 7.1 kg) representing a single professional football club across four analyzed seasons. The research was designed according to three methodological sets of assessments: (i) average season performance, (ii) two weeks’ performance before the event, and (iii) four weeks’ performance before the event. We applied machine learning classification methods to build and classify different predictive injury risk models for each dataset. The dependent variable is categorical, representing the occurrence of a time-loss muscle injury (N = 97). The independent variables include players’ information and external (GPS-derived) and internal (RPE) workload variables. Results: The Kstar classifier with the four-week window dataset achieved the best predictive performance, presenting an Area Under the Precision–Recall Curve (AUC-PR) of 83% and a balanced accuracy of 72%. Conclusions: In practical terms, this methodology provides technical staff with more reliable data to inform modifications to playing and training regimens. Future research should focus on understanding the technical staff’s qualitative vision of predictive models’ in-field applicability. Full article

Climate variability and extreme events disproportionately affect rural regions with limited adaptive capacity. In Minas Gerais, Brazil, mesoregions with semi-arid characteristics face severe vulnerabilities, underscoring the importance of detailed regional climate trend analyses. This study analyzed historical air temperature (maximum, minimum, and average) and precipitation from 1990 to 2019 in four mesoregions of Minas Gerais. The goal was to support climate planning and the development of local responses. Daily data from the National Institute of Meteorology (INMET) and a gridded meteorological database were analyzed using Mann–Kendall and Sen’s non-parametric tests, with a 95% confidence level (p-value ≤ 0.05) to identify significant trends. Annual results showed significant increases in maximum temperature in 15 of 24 evaluated areas, with rates from −0.03 to +0.15 °C year⁻¹. For minimum and average temperatures, significant increases were observed in 17 locations. Annual precipitation showed a downward trend in 21 areas. Monthly and seasonal analyses confirmed this pattern of warming and reduced rainfall. These findings indicate an intensification of climate stress in over 80% of the studied locations, potentially impacting agriculture, public health, and ecosystems, requiring specific regional adaptive responses. Full article

Background/Objectives: Organic UV filters are chemical compounds that are commonly used in sunscreen products to absorb UV radiation from the Sun. To date, the filters have been detected in aquatic environments worldwide, as well as in aquatic organisms, including fish and coral. Hydroxy-4-methoxybenzophenone (BP-3) is a common organic UV filter and it is well documented to be among the filters that are detectable worldwide in water samples and aquatic organisms. Long-term exposure in vivo studies have demonstrated that high doses of BP-3 can cause endocrine-disrupting effects in aquatic organisms. Methods: Using gonadal cell culture and quantitative RT-PCR, our study aimed to ascertain the effect of environmentally relevant doses of BP-3 (detected in aquatic systems) on the gene expression of reproductive targets, estrogen and growth hormone receptors (ERs and GHRs), in Mozambique tilapia (Oreochromis mossambicus) after an acute 24 h treatment. Results/Conclusions: Our study is the first to use an in vitro design to investigate the mechanism of the action of BP-3 on gonadal tissue in fish. Our results show that BP-3 does not induce gene regulation directly on the gonads of tilapia at doses that are comparable to what is detectable in aquatic environments after 24 h. We do verify, as seen in other teleost species, homologous regulation of ERβ in male tilapia gonadal tissue. Full article

Coffea racemosa and C. zanguebariae show promising characteristics for cultivation under stress conditions. However, their potential for breeding programs requires further characterization, especially regarding fruit attributes. This study aimed to characterize the bean/husk ratio and the nutrient content in bean and husks from 22 accessions of Coffea racemosa and another 22 of C. zanguebariae cultivated in Mozambique. Ripe fruits were collected, dried, and manually peeled to evaluate the percentage of bean and husk. The nutrient content (N, P, K, Ca, Mg, S, Fe, Zn, Cu, Mn, and B) was quantified separately by standard methodology. The data were summarized in scatter plots, and differences among accessions were analyzed using Euclidean distance and grouped following the Unweighted Pair Group Method with Arithmetic Mean. On average, beans accounted for 54.4% (ranging from 34.5% to 66.5%) of the fruit mass in C. racemosa and 60.4% (38.8% to 81.4%) in C. zanguebariae. Macronutrient content in beans followed the order N > K > Mg > P > S > Ca (average N = 19.98 kg ton⁻¹ of beans) in C. racemosa and N > K > Ca > Mg > S > P (average N = 25.42 kg ton⁻¹ of beans) in C. zanguebariae. Micronutrient content in beans followed the order Fe > B > Mn > Cu > Zn in both species, with average Fe content of 325.8 and 473.72 g ton⁻¹ of beans for C. racemosa and C. zanguebariae, respectively. No correspondence occurred between the bean and husk nutrient content. Coffea racemosa and C. zanguebariae exhibit bean proportions and nutritional profiles comparable to those of commercial species, highlighting their high potential for coffee diversification and genetic breeding. These results provide new evidence supporting the inclusion of C. racemosa and C. zanguebariae in breeding programs aimed at climate-resilient and nutritionally distinct coffee varieties. Full article

Waste dumpsites in developing countries are primary pollution sources impacting nearby ecosystems. This study assessed seasonal changes in soils surrounding the Hulene-B landfill (Maputo, Mozambique) and evaluated the potential for surface water contamination by leachates. A total of 71 samples were collected during the rainy and dry periods and analyzed for pH, electrical conductivity (EC), organic matter (OM), and color. The contamination potential (Pbci) was determined considering the landfill’s characteristics and local hydrological context. During the dry season, soils exhibited higher EC and OM, indicating greater retention of potentially toxic elements (PTEs) and a strong tendency for accumulation. In the rainy season, leaching processes prevailed, leading to reduced EC and OM but increased potential for contaminant mobility. The Pbci values were consistently high across both periods, confirming elevated contamination risk. Overall, the Hulene-B landfill exerts a marked influence on surrounding soils and nearby surface waters, underscoring the urgent need for structural measures to control leachate release and ash dispersion. Full article

The increasing global incidence of infections caused by free-living amoebae (FLA) and the lack of effective, safe, and approved treatments highlight the urgent need for novel amoebicidal compounds with pharmacological potential. Despite a growing body of literature on the anti-FLA properties of various compounds, comprehensive reviews summarizing this progress remain scarce. This study aimed to identify the most promising compounds tested in vitro and/or in vivo for anti-FLA activity. A systematic review was conducted, analyzing 108 studies published between 1986 and 2024, selected from an initial pool of 23,653 database results. A total of 537 compounds were evaluated for their in vitro anti-FLA activity. Compounds exhibiting ≥50% reduction in amoeba viability relative to untreated controls were classified as promising if they showed low toxicity in mammalian cell models, particularly when active at concentrations ≤ 10 µM, consistent with predicted favorable pharmacokinetic and pharmacodynamic profiles. The most promising compounds for drug and disinfectant development include ten trophocidal agents against B. mandrillaris, thirty-two trophocidal and four cysticidal agents against N. fowleri, and sixty-two trophocidal and nineteen cysticidal agents against Acanthamoeba spp. Compounds active at low concentrations (≤10 µM or <0.014 mg/mL) prioritized for in vivo drug development studies include: against Balamuthia mandrillaris, trophocidal 515, 531, 533; against Naegleria fowleri, trophocidal 421, 416, 518, 46, 254, 522, 111–120 and cysticidal 16; and against Acanthamoeba spp., trophocidal 498, 499, 500, 535, 107, 347, 348, and 340. Future studies should evaluate their efficacy, safety, pharmacokinetics, and pharmacodynamics toward developing effective drugs, antiseptics, and disinfectants. Full article

Background: Child stunting and wasting persist at alarmingly high rates in Mozambique, yet little is known about whether the improved sources of water and sanitation affect these outcomes differently. This study aims to disentangle the distinct contributions of improved water sources and sanitation facilities to child stunting and wasting at the national level, addressing a critical evidence gap in the WASH–nutrition literature in Mozambique. Methods: Using data from 3690 children under five in the 2022–2023 Mozambique Demographic and Health Survey, we applied stepwise logistic regression models to estimate the independent and combined associations of improved drinking water and sanitation facilities with child stunting and wasting, adjusting for child-, household-, and region-level factors. Results: Improved water access was significantly associated with a lower risk of stunting (odds ratio = 0.80, 95% CI: 0.67–0.94, p < 0.01), while sanitation showed only weak and inconsistent associations with stunting. In the fully adjusted model, neither improved water nor sanitation was associated with wasting. Wealth, gender, religion, and region were also significant predictors of stunting as well as wasting. Conclusions: These findings indicate that WASH components protect against child malnutrition through different pathways, with water being more protective against chronic undernutrition and sanitation less clearly linked to acute malnutrition. Broader socioeconomic and cultural factors—such as wealth, religion, and geography—play critical roles, highlighting the need for integrated, context-specific interventions. Full article

The quantification of genotype x environment interaction is essential for recommending high-yielding genotypes for both favorable and unfavorable environments, thereby increasing production. This study aimed to evaluate the agronomic performance of sweet potato genotypes in the central–east and central–south regions of São Paulo. The experiments were conducted using a randomized block design with 9 plants per plot and 3 replications, consisting of 18 sweet potato genotypes and 3 commercial cultivars, totaling 21 treatments. The characteristics, such as commercial productivity, dry matter, chroma, hue, insect resistance, eyes, and lenticels showed genotype x environment interaction for 77.78% of the variables. The maximum productivity of the genotypes ranged from 31.81 t/ha⁻¹ to 63.60 t/ha⁻¹. Heritability was observed in 88.89% of the analyzed traits, with values ranging from 75.36% to 93.47%, indicating a significant genetic influence on the evaluated characteristics. Location 4 (first cycle in Botucatu, 20 December 2021) was superior and considered the most suitable for sweet potato cultivation. The genotypes CERAT60-05, CERAT56-23, CERAT60-26, and CERAT35-11 performed best, showing promise as new cultivars. Full article

In most vertebrates, males and females display distinct reproductive behaviors. Some fish can change their sexual phenotype at various life stages, which involves alterations in their gonadal sex and changes in their reproductive behavior to align with the new gonadal identity. Although the sex reversal phenomenon in reproductive behavior is well documented, the underlying mechanisms in the brains of these fish remain largely unknown. In the present study, we investigated the roles of the thyroid hormone (triiodothyronine (T3)) in the Mozambique tilapia as a potential regulator of male-specific nest-building behavior and gonadotropin-releasing hormone-3 (GnRH3) neurons, the regulatory neurons of male reproductive behavior, in the terminal nerve (TN) ganglion. T3 injection successfully induced nest-building behavior in mature female fish. T3 injection significantly elevated serum T3 concentrations in treated animals compared with those in controls. Through organotypic culture of brain slices that included the TN region, we demonstrated that T3 could stimulate an increase in the number of GnRH3 neurons, and the effect was inhibited by a thyroid hormone receptor (TR) inhibitor. Additionally, TRβ co-expression was observed in GnRH3 neurons. These findings highlight the crucial roles of T3 and GnRH3 in sex reversal processes within the fish brain. Full article

Disaster headlines often underscore devastation and loss while overlooking success stories where proactive disaster risk reduction (DRRM) measures have averted catastrophe, saved lives, and reduced economic damage. This study addresses the gap in documentation and analysis of DRRM success stories in Africa, particularly within the Southern African Development Community (SADC), arguing that the absence of such narratives hampers a shift from reactive to proactive disaster risk governance. The research aims to extract critical lessons from success stories for enhancing future preparedness and response frameworks. A qualitative research design was employed, integrating document analysis, expert interviews, field observations, and practitioner workshops. Data was triangulated from diverse sources, including national disaster management agency reports (e.g., South Africa’s NDMC, Botswana’s NDMO, Mozambique’s INGC), peer-reviewed literature, UNDRR reports, SADC policy documents, and first-hand experiences from the authors’ consultancy work in the African Union’s biennial DRRM reporting processes. Case studies examined include Mozambique’s response to Cyclone Idai in 2019, South Africa’s drought and flood risk governance (e.g., the 2023 floods in Eastern and Western Cape), and Malawi’s flood resilience programs. Findings reveal that successful DRRM outcomes are driven by a combination of anticipatory governance, community-based preparedness, integration of Indigenous Knowledge Systems (IKSs), and investment in infrastructure and ecosystem-based adaptation. These cases demonstrate that locally embedded, yet scientifically informed, interventions enhance resilience and reduce disaster impacts. The study underscores the relevance of theoretical frameworks such as resilience theory, narrative theory, and social learning in interpreting how success stories contribute to institutional memory and adaptive capacity. Policy recommendations emphasize the need for institutionalizing success-story documentation in national DRRM frameworks, scaling up community engagement in risk governance, and fostering regional knowledge-sharing platforms within the SADC. Furthermore, the paper advocates for making DRRM success stories more visible and actionable to transition toward more anticipatory, inclusive, and effective disaster risk management systems. Full article