Search Results (4,023)

Background/Objectives: Low-grade systemic inflammation, characteristic of heart failure (HF), is a nonspecific inflammatory syndrome that affects the entire body. Macrophage migration inhibitory factor 1 (MIF-1) is a pro-inflammatory cytokine, a key mediator of the innate immune response, and may serve as a potential biomarker of monocyte homing and activation in HF with reduced and mildly reduced ejection fraction (HFrEF, HFmrEF). Methods: We evaluated 70 hemodynamically stable patients with left ventricular EF (LVEF) < 50% by means of echocardiography and blood sampling. Results: We report significant correlations between MIF-1, LVEF (r = −0.33, p = 0.005), LV global longitudinal strain (LVGLS, r = 0.41, p = 0.0004), and tricuspid annular plane systolic excursion (TAPSE, r = −0.37, p = 0.001). MIF-1 levels in HFrEF patients were relatively higher, but not significantly different from those observed in HFmrEF. MIF-1 showed significant associations with TAPSE to systolic pulmonary artery pressure ratio (TAPSE/sPAP, p < 0.0001). Also, patients with TAPSE/sPAP < 0.40 mm/mmHg had significantly higher levels of MIF-1 (p = 0.009). Moreover, ischemic cardiomyopathy (ICM) was more frequent in patients with MIF-1 concentrations above 520 pg/mL (57.1% MIF-1^hi vs. 28.6% MIF-1^lo, p = 0.029). In terms of congestion, MIF-1 showed significant associations with the presence of peripheral edema (p = 0.007), but none was found with self-reported dyspnea (p = 0.307) and New York Heart Association (NYHA) class (p = 0.486). Also, no relationship was reported with N-terminal pro-B-type natriuretic peptide concentrations (NT-proBNP, r = 0.14, p = 0.263). However, the six-minute walk distance was greater in individuals in the MIF-1^lo group when compared to those in the MIF-1^hi group (404.0 ± 127.4 vs. 324.8 ± 124.1 m, p = 0.010). Conclusions: Beyond identifying inflammatory biomarkers related to disease severity, linking MIF-1 to various pathophysiological mechanisms may highlight the active involvement of the monocyte-macrophage system in HF. This system holds notable significance in congestion-related conditions, acting as a major source of reactive oxygen species that perpetuate inflammation. Full article

Background/Objectives: The long-term effects of multiple updated vaccinations against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) have not been clarified. Humoral or cellular immunity dynamics in healthcare workers for four years were analyzed. Methods: Blood samples were collected at five time points from April 2021 to January 2024. Humoral immunity was analyzed using the 50% neutralizing titer (NT₅₀) against the original Omicron XBB and Omicron BA.2.86 strains and cellular immunity were analyzed using the ELISpot interferon-gamma releasing assay. NT₅₀s and the spot-forming count (SFC) of the ELISpot assay were compared in the SARS-CoV-2 Omicron XBB-, Omicron-infected, and uninfected subjects. Results: 32 healthcare workers (median age, 47 years) who received 3–7 vaccine doses were enrolled. The NT₅₀s against the original strain decreased after the second vaccination but were maintained after the third vaccine dose. NT₅₀s against the Omicron XBB and BA.2.86 strains were detected before the Omicron vaccine was introduced and increased following the updated vaccination. The NT₅₀s against the Omicron XBB and BA.2.86 strains were elevated after natural infection by the Omicron strain, albeit without differences compared with the findings in uninfected subjects. Multivariate regression analysis revealed no confounder that affected the antibody titer against the BA.2.86 strain at the fifth blood sampling. The median number of SFCs ranged from 78 to 208 after the first two doses. Conclusions: Multiple vaccinations induced the production of antibodies with divergent activity against emerging mutant strains and enhanced protective effects against the original strain. This finding supported the importance of updated vaccination. Full article

Universities and other institutes of higher education could be considered as key actors in the implementation of sustainability pillars, such as the adoption of sustainable practices in wastewater management. However, the adoption of such practices is still an emerging issue. This paper discusses the design and operation of the first combined Oxylag and high rate algal pond (COHRAP) constructed at the university campus in Tunisia for irrigation. Performance was evaluated based on the removal efficiencies of nutrients, chemical oxygen demand (COD), biochemical oxygen demand (BOD), heavy metals, coliforms, and biomass productivity. The potential reuse of sludge and algal biomass is discussed based on the Tunisian national standard regulation for sludge reuse in agriculture (NT 106.20) and the European regulation (EC, 2019/1009) for fertilizer products. Effluent phytotoxicity is tested on the germination and growth on Zea mays L. The results indicate that the COHRAP performance was globally satisfactory; however, biomass productivity (1.4 g m⁻²d⁻¹) was low, indicating the need for adjustments in the operational parameters. Despite the effluent limitations for TSS and Hg, no phytotoxic effect was observed. Regarding the heavy metal content in sludge and algal biomass, the results obtained were in compliance with NT 106.20 and EC, 2019/1009), respectively. The energy consumption of COHRAP is 1.05 kWh/m³ resulting in operational costs of 0.29 euros/m³. This study revealed that COHRAP could be a sustainable option to treat wastewater from university campuses with resource recovery. Such a choice can be improved by the implementation of an algae recovery step. Full article

This paper addresses the critical issue of selecting the optimal solar tracking configuration for maximum energy generation, given the increasing demand for sustainable energy solutions in information and communication technology (ICT) facilities. The main goal is to thoroughly evaluate and compare seven different solar tracking configurations across technical, economic, and environmental dimensions: No Tracking (NT), Monthly Adjusted Horizontal Axis (MAHA), Weekly Adjusted Horizontal Axis (WAHA), Daily Adjusted Horizontal Axis (DAHA), Continuously Adjusted Horizontal Axis (CAHA), Continuously Adjusted Vertical Axis (CAVA), and Dual Axis with Continuous Adjustment (DACA). This study utilizes the HOMER simulation program to evaluate its energy and hydrogen production, emissions, and cost-effectiveness performance. Key findings indicate solar tracking improves energy efficiency, with optimal capacity factors of 18.2% and 17.7% for CAHA and DAHA configurations, respectively. Although load-following strategies increase reliability, there is a trade-off between capital costs and energy costs. In addition, an MCDM approach helps to consolidate the evaluation, resulting in CAVA being ranked as the most preferable option. The study contributes to informed decision-making for energy systems in ICT facilities by emphasizing the significance of considering a variety of criteria and evaluation techniques to address complex energy challenges. Full article

Expansins are cell wall-modifying proteins that play a pivotal role in plant growth, development, and stress adaptation to abiotic stress. This manuscript explores the functions of expansins in salt and drought stress responses across multiple plant species, highlighting their involvement in cell wall loosening, transcriptional regulation, ion and osmotic homeostasis, and phytohormone signalling. Genome-wide identification and expression analyses revealed differential regulation of expansin genes under abiotic stress conditions. In Nicotiana tabacum, overexpression of NtEXPA4 and NtEXPA11 promoted root elongation and ion homeostasis, improving salt and drought tolerance. Similarly, Brassica rapa BrEXLB1 was found to modulate root architecture and phytohormone-mediated stress responses. In Oryza sativa, OsEXPA7 was linked to cation exchange and auxin signalling under salt stress conditions. Conversely, in Populus trichocarpa, PtEXPA6 exhibited a negative regulatory role in salt stress tolerance, highlighting species-specific differences in expansin function. Expansins also contribute to reactive oxygen species (ROS) homeostasis, as observed in transgenic plants with increased activities of SOD, POD, APX, and CAT, which reduced oxidative damage under stress. Additionally, enhanced accumulation of soluble sugars and proline in expansin-overexpressing plants suggests their involvement in osmotic adjustment mechanisms. The interplay between expansins and ABA, auxins, and ethylene further underscores their role in integrating mechanical and hormonal stress responses. Despite substantial progress, limitations remain in understanding the broader regulatory networks influenced by expansins. Future research should focus on elucidating their downstream molecular targets, transcriptional interactions, and functional diversity across different plant species. Expansins represent promising candidates for improving crop resilience to environmental stress, making them valuable targets for future breeding and biotechnological approaches. Full article

Botulinum toxin type A (BoNT-A), a potent neurotoxin, is increasingly used to treat myogenic temporomandibular disorders (TMDs); however, the interplay between muscle atrophy and pain relief remains incompletely understood. This pilot study investigated how masseter and temporalis muscle thickness and pain intensity change over 12 weeks following BoNT-A injections in 15 patients (mean age 51.42 years) with myogenic TMD. Muscle thickness was measured via ultrasonography across multiple anatomical positions under both clenching and resting conditions at baseline and at 2, 4, 8, and 12 weeks post-injection. Significant thinning of both muscles occurred within 2 weeks, lasting until 12 weeks, but became less pronounced after the first month. Pain intensity showed parallel decreases, most notably early on, but these reductions were not consistently statistically significant. Correlation analyses revealed no strong persistent association between muscle thickness and pain except for a moderately positive correlation in the anterior temporalis at two weeks (r = 0.61, p = 0.04). BoNT-A induces rapid masticatory muscle atrophy and modest pain relief; however, these outcomes do not coincide. Pain relief was observed earlier than the full development of muscle atrophy and should be considered during TMD pain management. Full article

Cardiac resynchronization therapy (CRT) using biventricular (BiV) pacing is the standard treatment for heart failure (HF) patients with reduced left ventricular ejection fraction (LVEF) and electrical dyssynchrony. However, one in three patients remains a non-responder. Left bundle branch area pacing (LBBAP) could represent a more physiological alternative, but its effectiveness is limited in cases of atypical left bundle branch block (LBBB) or intraventricular conduction delay (IVCD). Left Bundle Branch Pacing Optimized cardiac resynchronization therapy (LOT-CRT) integrates LBBAP with coronary sinus (CS) lead pacing to improve electrical synchrony and clinical outcomes. This review evaluates the feasibility, advantages, disadvantages, and clinical outcomes of LOT-CRT. Additionally, we describe our center’s experience and propose an evidence-based implantation algorithm. A review of published studies investigating LOT-CRT was conducted, comparing its effectiveness with BiV-CRT and LBBAP alone using QRS narrowing, LVEF improvement, left ventricular remodeling, New York Heart Association (NYHA) class changes and NT-proBNP levels. It was found that LOT-CRT outperforms BiV-CRT or LBBAP alone in selected populations, at the cost of higher clinical skills, longer procedural times, and specific device setups. Randomized trials are underway to further define its role in clinical practice. Full article

We compare enhancements of mesospheric volume mixing ratios of hydroperoxyl radical ${\mathrm{HO}}_2$ and nitric acid ${\mathrm{HNO}}_3$, as well as ozone depletion in the Northern Hemisphere (NH) polar night regions during energetic particle precipitation (EPP) in January of 2005 and 2012. We utilize mesospheric observations of ${\mathrm{HO}}_2$, ${\mathrm{HNO}}_3$, and ozone from the Microwave Limb Sounder (MLS/Aura). During the second half of January 2005 and 2012, the GOES satellite identified strong solar proton events with virtually the same proton flux parameters. Geomagnetic disturbances in January of 2005 were stronger, with Dst decreasing up to 100 nT compared to January 2012 while the Dst drop did not exceed 70 nT. Comparison of observations made with the MLS/Aura shows the highest change of ${\mathrm{HO}}_2$ and ${\mathrm{HNO}}_3$ concentrations and also the deepest ozone destruction at the latitudinal range from ${60}^{\circ }$ NH to ${80}^{\circ }$ NH inside the north polar vortex right after the spike in energetic particle flux registered by GOES satellites. MLS/Aura observations show ${\mathrm{HNO}}_3$ maximum enhancements of about 1.90 ppb and 1.66 ppb around 0.5 hPa (about 55 km) in January 2005 and January 2012, respectively. The ${\mathrm{HO}}_x$ increases lead to short-term ozone destruction in the mesosphere, which is seen in MLS/Aura ozone data. The maximum ${\mathrm{HO}}_2$ enhancement is about 1.05 ppb and 1.62 ppb around 0.046 hPa (about 70 km) after the onset of EPP in the second half of January 2005 and January 2012, respectively. Ozone maximum depletion is observed around 0.02 hPa (about 75 km). Ozone recovery after EPP was much faster in January 2005 than in January 2012. Full article

Optically pumped magnetometers (OPMs) are a promising magnetoencephalography (MEG) technology for the non-invasive measurement of human electrophysiological signals. Prior work developed biplanar background field-nulling coils necessary for OPM operation, but these were expensive to produce and required tedious error-prone manual winding of >1 km of copper wire. Here, we developed a precise and reproducible manufacturing process by fabricating these coils on two-layer printed circuit boards (PCBs). Building on open-source software (bfieldtools), we developed a pipeline to determine the optimal current loops of 1.5 × 1.5 m² biplanar nulling coils, connected these loops into a continuous conducting path across PCB layers, and printed them as pairs of 1.5 × 0.75 m² PCBs, which were soldered and mounted on an aluminum frame. Our coils achieved efficiencies of 1.3–7.1 nT/mA, similar to or higher than previous designs. We reduced the largest background field component from 21 to 2 nT, enabling OPMs in a lightly shielded room to record somatosensory evoked fields (SEFs) comparable to SQUID-MEG. Our coil system is cheaper than commercial alternatives and is available as an open-source package opmcoils, thus enabling more affordable background field nulling for OPM-MEG and realizing its potential as an accessible sensor technology for human neuroscience. Full article

Background/Objectives: Patients with transthyretin amyloid cardiomyopathy (ATTR-CM) often experience significantly reduced functional capacity due to myocardial involvement. Cardiopulmonary exercise testing (CPET) is the gold standard to quantify functional capacity, and ^99mTc-DPD scintigraphy and SPECT/CT have proven to be highly effective tools for diagnostic and disease monitoring. We aimed to investigate the complementary role and correlation between both methods, focusing on their combined potential as a strong prognostic framework for monitoring disease progression and evaluating treatment efficacy. Methods: A total of 44 patients with diagnosed ATTR-CM, who underwent ^99mTc-DPD scintigraphy and SPECT/CT imaging as well as CPET, were included. All patients were divided into two groups based on the median DPD retention index (low DPD uptake: ≤5.0, n = 22; high DPD uptake: >5.0, n = 22). Results: The mean age was 78 years, with 82% of participants being male. Significant correlations were observed between peak VO₂ and DPD retention index (r = −0.355, p = 0.018) as well as between peak VO₂ at anaerobic threshold with DPD retention index (r = −0.391, p = 0.009). Interestingly, there was no strong correlation between VE/VCO₂ slope and the retention index. A strong association was identified between cardiac biomarkers and peak VO₂, specifically for NT-proBNP (r = −0.530, p < 0.001) and Troponin T (r = −0.431, p < 0.001). Conclusions: In ATTR-CM, significant correlations were observed between key CPET parameters and quantitative cardiac DPD uptake, which further reflects on disease severity and functional impairment. Our findings highlight the utility of integrating CPET and SPECT/CT for comprehensive patient assessment in ATTR-CM. Full article

Studies on the presence of the Old Testament (OT) in the New Testament (NT) have evolved significantly over time. Scholars have moved from a focus on identifying the textual version employed by the evangelists to attempts to systematize the various levels of the OT’s presence, classifying them as quotations, allusions, or echoes. In reality, biblical references permeate the NT, often going unnoticed. Unveiling them and, above all, approaching them through the interpretative logic characteristic of Judaism at the turn of the era imparts a surplus of meaning to the NT text. This is what this article demonstrates through an example from the Gospel according to Mark. There is no doubt about the strong biblical resonances in the passage describing Jesus’ entry into Jerusalem (Mk 11:1-11). In addition to a quotation from Psalm 118 (Mk 11:9-10), scholars recognize an allusion to Genesis and multiple references to texts from the book of Zechariah. This article will illustrate how drawing upon Jewish exegetical tradition from the turn of the era sheds light both on the way these OT references are interpreted and on how the various biblical references interconnect, enriching and expanding the meaning of the Gospel passage. Full article

Global climate change presents a significant challenge to aquatic ecosystems, with ectothermic fish being particularly sensitive to temperature fluctuations. The brain plays a crucial role in perceiving, regulating, and adapting to thermal changes, and its response to heat stress is crucial for survival. However, the molecular mechanisms underlying heat stress and acclimation in fish brains remain poorly understood. This study aimed to investigate the adaptive mechanisms of Hong Kong catfish (Clarias fuscus) brains under heat acclimation and acute heat stress using transcriptome analysis. Fish were divided into two groups: a normal temperature group (NT, 26 °C for 90 days) and a heat-acclimated group (HT, 34 °C for 90 days), followed by acute heat stress (34 °C for 72 h) and recovery (26 °C for 72 h). Heat acclimation improved C. fuscus tolerance to acute heat stress, with faster gene responses and stronger neuroprotection. Key pathways enriched included cell adhesion and ECM-receptor interactions during recovery. Apoptosis regulation was balanced, with the HT group upregulating anti-apoptotic genes to mitigate neuronal cell death. Additionally, the lysosome–phagosome pathway was activated during recovery, facilitating the transport of lysosomal enzymes and the clearance of damaged cellular components, aiding neuronal repair. Ribosome biogenesis was suppressed under heat stress to conserve energy, but this suppression was less pronounced in the HT group. In summary, heat acclimation enhances neural protection in C. fuscus brains by promoting neuronal repair, suppressing apoptosis, and activating lysosomal pathways, thereby improving tolerance to acute heat stress. These findings offer a molecular basis for breeding heat-tolerant fish species in aquaculture, and deepen our understanding of thermal adaptation in aquatic animals amid global climate change. Full article

Silicon-based storage technologies are increasingly failing to meet the explosively growing data storage demands of the information age. DNA-based data storage offers a promising solution due to its unparalleled storage density, long lifespan, low energy consumption, and high parallel accessibility. In this study, we propose a novel True Quadratic Codec System (ETQ) that directly encodes data into nucleotide sequences using a quaternary encoding approach. By treating A, T, C, and G as direct encoding symbols (0, 1, 2, 3), an ETQ eliminates the intermediate binary-to-ATCG conversion step, thus surpassing the theoretical storage density limit of 2 bits/nt. An ETQ is built on these three key components: (1) dividing image data into B, G, and R color channels for separate encoding and storage, (2) employing quaternary Huffman encoding to map image information directly into nucleotide sequences, and (3) integrating Reed–Solomon error correction codes to enhance data reliability and system extensibility. The ETQ framework demonstrates significant improvements in storage density and efficiency compared to conventional methods. By leveraging the inherent properties of DNA, this system offers a scalable and cost-effective solution that addresses the growing global data storage crisis. Full article

Osteoarthritis (OA) is the most prevalent rheumatologic disease and a leading cause of years lived with disability worldwide. There are no disease-modifying drugs available to treat it. This study aimed to evaluate the effect of a single dose of 100U botulinum neurotoxin-A (BoNT-A) in patients with early knee OA. We designed a single-arm preliminary clinical trial in patients diagnosed with knee OA (KOA) grades I and II. 45 Patients received a single dose of 100U IncobotulinumtoxinA in the retro-patellar bursa and received nutritional and physical rehabilitation indications. Patients were evaluated at baseline and at days 5, 30, 60, and 90 after injection. The primary outcome was the reduction in pain using the visual analog scale (VAS). Knee function was evaluated using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). We assessed secondary adverse effects and measured muscular strength in every consultation. Descriptive endpoint summaries and a generalized linear random-effect model were used to evaluate changes in each follow-up time compared to baseline. IncobotulinumtoxinA treatment significantly (p < 0.001) reduced pain in all treated patients at day 90 compared to day 0. Patients showed a significant reduction in total WOMAC score (p < 0.001), from a mean baseline of 44.6 (95% CI; 41.4, 47.8) to 4.4 at day 90 (95% CI; 0.2, 0.3). Our results show that IncobotulinumtoxinA applied in the retro-patellar bursa is a safe and effective treatment for pain in patients with early-stage KOA, offering a potential alternative for symptomatic control in KOA. Full article

Background/Objectives: Management of complex congenital heart defects with functionally single ventricle remains one of the greatest challenges of pediatric cardiology. The multistage surgical treatment completed with Fontan procedure is related to multiple complications. Due to non-pulsatile continuous pulmonary flow and chronic hypoxia, Fontan circulation may induce pulmonary endothelial dysfunction. However, the impact of Fontan physiology on respiratory system function is not well studied. The aim of the research was to assess respiratory function in Fontan pediatric patients with hypoplastic left heart syndrome (HLHS) and other morphologies of functionally single ventricle. The article presents the preliminary results drawn from the pilot study, focusing on Fontan patients, without a healthy children control group. Methods: A cross-sectional study involved Fontan patients hospitalized in the Pediatric Cardiology Clinic of the Medical University of Silesia in Katowice between August 2023 and November 2024. The exclusion criteria were lack of parental and/or patient’s consent, age < 6 years old, decompensated heart failure, asthma, atopy, respiratory infection within two weeks before the hospitalization, or significant psychomotor disability. Respiratory function assessment involved spirometry and fractional exhaled nitric oxide (FeNO) measurement. Results: A total of 32 patients who met inclusion criteria performed respiratory measurements. The mean age was 12.9 years old; there were 12 females. A total of 12 patients had HLHS and 20 patients had other morphologies of univentricular heart. FeNO values were relatively high with a mean of 30 ppb. Spirometry showed restrictive or mixed restrictive and obstructive ventilatory pattern. The mean forced vital capacity (FVC) levels were 79.2 ± 12.3% of predicted value (%pv) and forced expiratory volume in the first second (FEV1) 77.3 ± 13.8%pv. Children with HLHS presented statistically significantly lower percentages of predicted value of FEV1. There were statistically significant negative correlations between NT-proBNP concentrations and FEV1, FEV1%pv, MEF25-75 and MEF25-75%pv. Conclusions: Fontan pediatric patients present a restrictive or mixed restrictive and obstructive ventilatory pattern and relatively high FeNO levels. Patients with HLHS have worse pulmonary function than patients with other univentricular heart morphologies. This may be related to worse ventricular function in patients with HLHS. Full article