Search Results (2,649)

The white poplar (Populus alba L.) is an economically significant tree species with a natural distribution spanning an extensive region of Eurasia. Nevertheless, there is currently no hypothesis regarding the historical shaping of this range. In this study, we collected and sequenced 36 individuals of white poplar from disparate regions of Russia and Kazakhstan. Additionally, we employed available genomic data of white poplars from Italy, Hungary, and China. A genomic approach was employed to collate data on the location of glaciers in different periods, along with information on the natural and artificial distribution of white poplar. This enabled the formulation of the first hypothesis regarding the formation of the modern range of this plant. It is hypothesized that during the period of maximum glaciation, three refugia existed: the South European, Transcaucasian, and Altai–Middle Asian refugia. Postglacial migration from these refugia led to the formation of modern populations of P. alba in Eastern Europe (including the European part of Russia), the Caucasus, and Siberia, respectively. Full article

The characterization of lithology within Quaternary aquifers holds significant geological importance for the protection, management, and utilization of groundwater resources, yet it continues to present considerable challenges. Indicator Kriging (IK) is a non-parametric, probability-based method of spatial interpolation. It considers the correlation and variability between data points, and its popularity stems from its alignment with geological experts’ principles. However, it still encounters issues in complex geological conditions. To address the limited capacity of conventional IK in reproducing geological variables within heterogeneous geological settings, this study develops an ordered IK method incorporating field variogram function parameters. This framework dynamically extends IK applications by integrating stratigraphic extension trends, requiring experts to formalize spatial variation trends into geological knowledge data, subsequently transformed into constraint parameters for interpolation. Estimation paths are determined via Euclidean distances between points-to-be-estimated and valid data, executing ordered IK following near-to-far and bottom-to-top principles. Results directly depict QLS formation spatial distributions or undergo expert modification for quantitative analysis, demonstrating superior integration of geological knowledge compared to empirical variogram fitting and partitioned IK estimation. The method reduces deviation from expert-interpreted spatial distributions while maintaining computational efficiency and multi-factor integration, with three case analyses confirming enhanced accuracy in lithology distribution reproduction and improved geostructural congruence in complex geological reconstruction. This approach revitalizes Kriging applications in complex geological research, synergizing domain cognition with computational efficacy to advance precision in geological characterization and support government decision-making. Full article

Background/Objectives: Chordoma, a rare slow-growing malignant bone tumor, remains therapeutically challenging due to its invasive nature. We examined institutional outcomes comparing imatinib and brachyury-directed vaccines. Methods: We used data from three sites of our quaternary care academic institution to analyze demographics, time to progression, overall survival, and adverse events in chordoma patients treated with imatinib or enrolled in a brachyury vaccine trial. Results: We included a total of 52 patients (8 in the brachyury vaccine cohort and 44 in the imatinib cohort) in the analysis. As expected, sacrococcygeal location was the most predominant in both cohorts: 62.5% in the brachyury cohort and 45.5% in the imatinib cohort. No demographic differences were present between the cohorts. ECOG was similar in both groups (p = 0.796). The primary outcome, time to progression (TTP), was shorter in the brachyury compared to the imatinib cohort (5.6 vs. 13 months, p = 0.0589), almost reaching statistical significance. Overall survival (OS) was comparable, 211 vs. 212 months for the brachyury and imatinib cohorts, respectively (p = 0.277). Although the brachyury vaccine cohort presented a higher incidence of adverse events than the imatinib cohort (75% vs. 31.8%, p = 0.021), the severity was milder. Conclusions: Imatinib showed longer disease control than the brachyury vaccine, though overall survival was similar. Future studies and deeper molecular insights are essential to develop better therapies and improve patient quality of life. Full article

Climate change and intensive urbanisation contribute to an increase in groundwater temperatures, leading to the development of the subsurface urban heat island (SubUHI) and Groundwater Urban Heat Island (GUHI) phenomenon. This article presents the results of comparative studies conducted in Wrocław (Poland) in two measurement campaigns in 2004–2005 and 2022–2024. The study aimed to assess long-term thermal changes in groundwater in relation to air temperature using statistical and spatial analyses. The data were processed using descriptive methods, interpolation (ordinary kriging), map algebra, and correlation analysis (Pearson). The results indicate a clear warming of groundwater, especially in the winter season, where the average temperature increase ranged from 2 to 4 °C compared to the first measurement period. The most substantial changes were observed in shallow Quaternary aquifers, showing a strong correlation with air temperature (r ≈ 0.8). Spatial analyses revealed the major groundwater temperature (GWT) increases in the city centre and densely built-up districts, confirming the intensification of the GUHI effect. In addition, a decrease in the seasonal amplitude of GWT and a local lowering of the water table were observed. Full article

The Messara Basin, a critical agricultural region in Crete, Greece, faces escalating geohazards, particularly land subsidence driven by intensive groundwater abstraction. Historical radar interferometry (1992–2009) indicated subsidence up to 20 mm·yr⁻¹, while recent European Ground Motion Service data (2016–2021) show mean vertical velocities reaching −31.2 mm·yr⁻¹. This study provides the first integrated hydrogeological assessment for the Basin, based on systematic field surveys, borehole inventories, and four coordinated campaigns (2021–2023) that established a basin-wide monitoring network of 767 stations. The dataset supports delineation of recharge zones, identification of potentiometric depressions, and mapping of aquifer-stress areas. Results show strong seasonality and extensive cones of depression, with local heads declining to ~−50 m below sea level. Land-use change (1990–2018 CORINE data; 2000–2020 agricultural censuses) combined with updated geological mapping highlights the vulnerability of post-Alpine formations, especially Quaternary and Plio–Pleistocene deposits, to deformation. The combined evidence links pumping-induced head decline with spatially coherent subsidence, delineates hotspots of aquifer stress, and identifies zones of elevated compaction risk. These findings provide a decision-ready baseline to support sustainable groundwater management, including enhanced monitoring, targeted demand controls, and managed aquifer-recharge trials. Full article

Quantitative investigation of major fault zones with low slip rates and long recurrence intervals in densely populated regions is essential for understanding earthquake recurrence and assessing seismic hazard. The Tanlu Fault Zone, a major lithospheric boundary extending from eastern China into Russia, provides a key case study. Through remote sensing interpretation integrated with seismic-geological evidence, we identified a ~150 km-long fresh surface rupture zone along the Yilan–Yitong Fault in the Fangzheng–Tangyuan region of Heilongjiang Province, NE China. Chronological constraints from previous and recent trenching indicate that the most recent event occurred in the late Holocene, with an estimated magnitude of M_w ≈ 7.6, comparable to the scale of AD 1668 Tancheng earthquake in North China. The northeastern section of the Tanlu Fault Zone is also subject to long-term far-field Coulomb stress loading from subduction of the Pacific Plate beneath the Eurasian Plate. Although the fault exhibits long recurrence intervals, the urgency of future strong earthquakes cannot be overlooked. Furthermore, our results suggest that the northeastern Tanlu Fault Zone is characterized by segmentation, underscoring the need for refined paleoseismic investigations to constrain recurrence behavior and seismic hazard in Northeast China. Full article

Dimethacrylate-based dental materials are dominantly used in restorative procedures for their mechanical and esthetic properties. However, they lack inherent antimicrobial activity, making them susceptible to microbial colonization. This has prompted the development of quaternary ammonium dimethacrylate monomers (QADMs) as a counteractive measure. This review critically assesses the tradeoffs associated with the antimicrobial potential, cytocompatibility, and structural performance of QADMs in the past decade. Across the standardized biological assays studied, QADMs consistently exhibit potent antimicrobial activity against cariogenic and opportunistic pathogens without inducing resistance. QADMs maintain favorable mechanical and physicochemical properties upon incorporation into resin composite formulations. Cytotoxicity is structure- and dose-dependent; nonetheless, most QADMs are biocompatible at antimicrobial concentrations. Notably, quaternary ammonium urethane dimethacrylate monomers (QAUDMAs) offer a balanced combination of antimicrobial and structural properties. Few studies have assessed the long-term mechanical durability of QADM-enhanced composites, leaving clinical relevance inconclusive. Further research is necessary to optimize monomer design and clinically validate these materials. Full article

Extracellular particles (EPs), an umbrella term encompassing membrane-enclosed extracellular vesicles (EVs) and non-vesicular extracellular particles ([NVEPs], previously described as extracellular condensates [ECs]) contain a complex cargo of biomolecules, including DNA, RNA, proteins, and lipids, reflecting the physiological state of their cell of origin. Identifying proteins associated with EPs that regulate host responses to physiological and pathophysiological processes is of critical importance. Here, we report the findings of our study to gain insight into the proteins associated with NVEPs. We used samples from human semen, the rat brain, and the rhesus macaque (RM) brain and blood to assess the physical properties and proteome profiles of NVEPs from these specimens. The results show significant differences in the zeta potential, concentration, and size of NVEPs across different species. We identified 938, 51, and 509 total proteins from NVEPs isolated from rat brain tissues, RM blood, and human seminal plasma, respectively. The species-specific protein networks show distinct biological themes, while the species-conserved protein interactome was identified with six proteins (ALB, CST3, FIBA/FGA, GSTP1, PLMN/PLG, PPIA) associated with NVEPs in all samples. The six NVEP-associated proteins are prone to aggregation and formation of wide, insoluble, unbranched filaments with a cross-beta sheet quaternary structure, such as amyloid fibrils. Protein-to-function analysis indicates that the six identified proteins are linked to the release of dopamine, immune-mediated inflammatory disease, replication of RNA viruses, HIV-HCV co-infection, and inflammation. These interesting findings have created an opportunity to evaluate NVEPs for their potential use as biomarkers of health and disease. Additional in-depth studies are needed to clarify when and how these proteins sustain their physiological role or transition to pathogenic roles. Full article

Quaternary catchment X22J boasts ecological biodiversity, making ecotourism one of the thriving industries in the catchment. However, recent population growth and the migration from rural areas to urban areas have increased urbanisation. Therefore, this study aimed to assess and predict the trajectory of urban growth. Through the random forest algorithm in Google Earth Engine, this study analysed urban use in 1990, 2007 and 2024. The classification achieved an overall score of 0.89, 0.96 and 0.91 for 1990, 2007 and 2024, respectively. In addition, the Kappa coefficient varied between 0.85, 0.83 and 0.87 for 1990, 2007 and 2024. The CA–MLP–ANN algorithm was applied for the prediction of 2040 urban changes, leading to the model achieving a score of an overall Kappa coefficient of 0.52 and 74% correctness. Overall, the study predicted an increase of 4.01% in built-up areas from 2024 to 2040, maintaining the increasing trend from 1990. Consequently, a loss of 11% was observed in agricultural lands and a loss of 0.17 in waterbodies by 2040. Full article

Virtual commands serve as the essential framework for establishing interaction between the virtual pilot and the MCP in autopilot scenarios. Conventional proportional-integral-derivative (PID) controllers are insufficient to ensure accurate flight trajectories due to system hysteresis. To overcome this limitation, a quaternary correlation prediction compensation PID (QCPC-PID) approach is introduced for computing virtual heading commands in autopilot tasks. The method integrates multi-feature statistics, entropy-based predictive compensation, and quaternary correlations. First, flight trajectory error statistics are dynamically calculated using signed error distances to assess deviation levels. Second, a predictive structure based on information entropy is applied to enhance PID compensation. Third, quaternary correlation dependence is established to generate virtual heading commands. The findings confirm the effectiveness of the method in improving flight convergence. The incorporation of predictive structures and quaternary correlations is critical for achieving predictive compensation during PID tuning, thereby reducing flight trajectory deviations. The quaternary correlation prediction compensation method ensures superior performance of PID control in modeling heading adjustment behavior under autopilot conditions. Full article

The Carpathian Basin holds exceptional significance for Quaternary research, particularly in loess studies. In this study, we attempted to create age-depth models based on age data from scientific journals to investigate accumulation rates. We examined eleven open profile sections for loess and paleosol, including seven in Hungary, two in Croatia, and two in Serbia. We demonstrated that radiocarbon age data are much more useful and reliable than OSL/IRSL data for this type of investigation. The results indicate that the Pécel, Dunaszekcső, Madaras and Katymár sections exhibit accumulation rates an order of magnitude higher than the other sections, exceeding one millimeter per year. These findings suggest that, owing to the basin’s geographic position, these areas were consistently exposed to dust deposition, irrespective of changes in climate or wind direction. A secondary accumulation maximum was also detected along the Katymár–Surduk axis, indicating an additional phase of intensified sediment deposition within this transect. The absence of a young sediment maximum in the Máza section is interpreted as resulting from a shift in prevailing wind direction, which caused the incoming dust to be intercepted by the Mecsek Mountains. Full article

Background: Periodontally accelerated osteogenic orthodontics (PAOO) is a surgical procedure to accelerate orthodontic tooth movement and minimize periodontal complications. This study surveyed U.S. periodontists to assess various aspects of the procedure as regards prevalence, training, and execution. Methods: The authors developed a unique questionnaire, the first national study of this type, housed on the Qualtrics^® survey platform, to analyze trends in PAOO training and use. Unique recruitment emails were sent to 3154 members of the American Academy of Periodontology. 449 U.S. periodontists/3154 surveyed (14.2%) responded to this web-based, anonymized survey. IBM statistical software (SPSS V28) was used for data analysis. Results: Among respondents, PAOO training was received during residency (32.7%) and by continuing education (CE) (50.8%), with higher CE (57.3%) by those who did not receive PAOO residency training (p < 0.001). 38.5% of periodontists perform PAOO, and those most likely to perform PAOO had both PAOO residency training and CE, with 78.5% performing 1–5 cases/year. Most (87.7%) received 1–2 PAOO referrals/year from orthodontists or general dentists. Differences in techniques and materials were the type of bone graft or membrane used, the position of corticotomies, and the timing of orthodontic movement. The primary PAOO goal was “rapid tooth movement” (41.1%) and to “increase the alveolar housing” (37.2%). The secondary (38%) and tertiary (37.2%) ranked goals were “augment dehiscence or fenestration”, with the “prevention of apical root resorption” ranked as their quaternary goal. Conclusions: The results of this survey provide data on the trends, training, and use of PAOO among U.S. periodontists. This information may aid in developing residency curriculum and performing PAOO research. Full article

Interleukin-6 (IL-6) is a central mediator of systemic inflammation and is markedly elevated in critical illnesses, including sepsis, acute respiratory distress syndrome, and hyperinflammatory syndromes. Patient responses to immunomodulatory therapies vary, highlighting the need to better understand IL-6 regulation and its clinical implications. We retrospectively analyzed consecutive patients admitted to a medical intensive care unit in a quaternary academic center with a comprehensive cancer program, extracting clinical and laboratory data, including inflammatory markers and plasma IL-6 levels. Plasma IL-6 concentrations were measured using an electrochemiluminescence immunoassay. Survival analyses, multivariable adaptive Lasso regression, Bayesian logistic regression, and latent class analysis were performed to define determinants of IL-6 regulation, mortality, and inflammatory phenotypes. IL-6 levels were substantially elevated in sepsis (median 1150 pg/mL) and neutropenia (median 7866 pg/mL), with extreme concentrations exceeding 20,000 pg/mL when both were present. Although IL-6 across its full range was not independently predictive of intensive care unit mortality, dichotomized thresholds (≥200 pg/mL) correlated with lower survival. Advanced modeling defined a hyperinflammatory phenotype characterized by IL-6 ≥ 100 pg/mL and predicted mortality >40%, showing mortality of 58%, alongside distinct latent subgroups with heterogeneous inflammatory activity and outcomes. These results emphasize the prominent role of sepsis and neutropenia in driving IL-6 elevations and reveal inflammatory phenotypes with potential for risk stratification and targeted anti-cytokine therapy in critical illness. Full article

The Early Pleistocene continental deposits of the Tiberino Basin (Central Italy) host exceptionally preserved fossil charophyte assemblages that provide critical insights into palaeoenvironmental and palaeoclimatic dynamics during a key phase of the Mediterranean evolution. Integrated micropalaeontological and sedimentological investigations at three reference sections reveal distinct charophyte communities characterized by Chara cf. hispida (Hartman) Wood, 1962, Chara cf. vulgaris Linnaeus, 1753, Nitellopsis obtusa (Desvaux in Loiseleur) Groves, 1919, and Lychnothamnus barbatus (Meyen, 1827) von Leonhardi 1863, not reported until now. These assemblages reflect a mosaic of stable lacustrine, ephemeral swamp, and palustrine environments shaped by increasing climatic oscillations approaching the Early–Middle Pleistocene transition. Comparative data from Mediterranean basins, such as Laguna de Gallocanta, Lake Afourgagh, and Ilgin Palaeolake (Türkiye), highlight the role of charophytes as sensitive indicators of hydrological and climatic variability. This study strengthens the palaeolimnological and palaeoenvironmental significance of charophyte fossils and proposes new avenues for multidisciplinary research into Quaternary environmental evolution in Mediterranean continental basins. Full article

Background: Recombinant monoclonal antibodies (mAbs) represent the fastest-growing sector of the biopharmaceutical industry, with their efficient expression being a key technological factor for scalability. Objectives: In this study we compared the performance of two bicistronic vectors, which alternate the positions of the light and heavy chain coding genes, employing a wild-type Encephalomyocarditis virus (EMCV) IRES functional element to drive expression of the second gene. Methods: Using two neutralizing anti-SARS-CoV-2 IgG1 antibodies as model molecules, we conducted transient transfections in the commercially available ExpiCHO^TM platform. Following protein A affinity purification and quantification, vectors positioning the light chain as the first cistron consistently yielded higher expression levels than those with the heavy chain upstream. To confirm the quality attributes of the mAbs, we applied a comprehensive analytical workflow, including SDS-PAGE and Western blot for molecular mass and purity, circular dichroism for secondary structure, intrinsic tryptophan fluorescence for tertiary structure, and SEC-HPLC for quaternary structure and aggregate detection. Additionally, we assessed binding affinity to the target using spot blot and surface plasmon resonance, analyzed N-glycosylation profiles by HILIC-HPLC and mass spectrometry, and examined molecular structure by transmission electron microscopy. Results and Conclusions: Together, these results provide insight into the impact of gene positioning within bicistronic vectors on mAb expression efficiency and quality, supporting optimization strategies for scalable recombinant antibody production. Full article