Search Results (936)

Background/Objectives: Sepsis is a life-threatening condition characterized by an uncontrolled immune response due to systemic infections. It is responsible for millions of deaths worldwide. Although inflammasomes play an important role in host defense, they have a detrimental role in sepsis induced by LPS or Gram-negative bacteria. We aimed to revise the molecular mechanisms of inflammasome activation in sepsis by LPS and Gram-negative bacteria other than cytokine release as treatments blocking TNF-α and IL-1 cytokines have been ineffective even though cytokine storm is associated with lethality. Results: Studies with knockout mice deficient in inflammasome-derived cytokines have shown contrasting results on the role of these proinflammatory cytokines in the lethality of LPS- and Gram-negative-induced sepsis. However, DAMPs released after non-canonical inflammasome activation such as extracellular DNA, histones, HMGB1, and tissue factor result in disseminated-intravascular coagulation (DIC) and mortality in mice. Blocking these products in preclinical studies with animal models showed improved clinical scores and survival after LPS-induced sepsis or polymicrobial sepsis induced by Cecal Ligation and Puncture. Conclusions: Even though immunomodulatory drugs have shown inconclusive results as therapies for sepsis, blocking DAMPs associated with DIC may be considered for clinical trials in the future, especially in patients presenting biomarkers of coagulopathies. Full article

Flooding from hurricanes creates damp indoor environments that support mold growth and microbial contamination, posing long-term health risks for occupants. This pilot study evaluated TMVOCs, microbial activity, and environmental conditions in 13 Hurricane Ian-affected residences across multiple flood-affected neighborhoods. Air samples were collected using sorbent tubes and analyzed by gas chromatography–mass spectrometry, while microbial activity on surfaces was assessed via ATP bioluminescence. Visible mold and dampness were documented with the CDC/NIOSH Dampness and Mold Assessment Tool, and environmental measurements included temperature, relative humidity, and surface as well as hidden moisture. Median (IQR) TMVOC concentrations were 12 (8) µg/m³, with 61% of homes exceeding the 10 µg/m³ benchmark set by previous researchers despite minimal visible contamination. Spearman’s correlation revealed significant negative relationships between odor and surface microbial activity (ρ = −0.569, p < 0.05), indicating that organic debris may play a more crucial role in microbial activity within the tested homes, and that odors might originate from hidden microbes instead of surface microbial growth. Our study emphasizes the necessity of utilizing both chemical (TMVOC) and biological (ATP) indicators to evaluate poor air quality caused by molds in flood-affected homes, serving as a supplement to routine visible mold assessments. Full article

The suspension system can adapt to different road excitations by adjusting its own stiffness or damping, or outputting active driving force, thereby improving the comprehensive dynamic performance of the vehicle, including ride comfort and vehicle handling. As the automotive industry’s requirements for “intelligence, comfort, and safety” continue to increase, the intelligence of suspension systems has become a research hotspot for scientific research institutions and enterprises, with broad development prospects. This article reviews the current development status of automotive suspensions and introduces the working principles and research status of different types of suspension systems, such as passive suspensions, semi-active suspensions, active suspensions, and electromagnetic suspensions. In addition, it summarizes the control methods of vehicle intelligent suspensions, including classical control, modern control, and intelligent control, and expounds the advantages and disadvantages of each control strategy. Finally, it summarizes the challenges and development trends faced by suspension systems. This review can provide technical reference for researchers engaged in the study of intelligent suspension under the modern chassis architecture and offer direction guidance for the development of key suspension technologies. Full article

While synthetic biology has traditionally focused on creating biological systems often through genetic engineering, emerging technologies, for example, implantable pacemakers with integrated piezo-electric and tribo-electric materials are beginning to enlarge the classical domain into what we call symbiotic synthetic biology. These devices are permanently attached to a body, although non-living or genetically unaltered, and closely mimic biological behavior by harvesting biomechanical energy and providing functions, such as autonomous heart pacing. They form active interfaces with human tissues and operate as hybrid systems, similar to synthetic organs. In this context, the present paper first presents a short summary of previous in vivo studies on piezo-electric composites in relation to their deployment as battery-less pacemakers. This is then followed by a summary of a recent theoretical work using a damped harmonic resonance model, which is being extended to mimic the functioning of such devices. We then extend the theoretical study further to include new solutions and obtain a sum rule for the power output per cycle in such systems. In closing, we present our quantitative understanding to explore the modulation of the quantum vacuum energy (Casimir effect) by periodic body movements to power pacemakers. Taken together, the present work provides the scientific foundation of the next generation bio-integrated intelligent implementation. Full article

Human cytomegalovirus (HCMV) establishes lifelong latency following primary infection, residing within myeloid progenitor cells and monocytes. To achieve this, the virus employs multiple immune evasion strategies. It suppresses innate immune signaling by inhibiting Toll-like receptor and cGAS-STING pathways. In addition, the virus suppresses major histocompatibility complex (MHC)-dependent antigen presentation to evade T cell recognition. As the downregulation of MHC molecules may trigger NK cell activation, the virus compensates for this by expressing proteins such as UL40 and IL-10, which engage inhibitory NK cell receptors and block activating signals, thereby suppressing NK cell immune surveillance. Viral proteins like UL36 and UL37 block host cell apoptosis and necroptosis, allowing HCMV to persist undetected and avoid clearance. In settings of profound immunosuppression, such as after allogeneic hematopoietic stem cell transplantation (allo-HSCT) or solid organ transplantation, slow immune reconstitution creates a window for viral reactivation. Likewise, immunosenescence and chronic low-grade inflammation during aging increases the risk of reactivation. Once reactivated, HCMV triggers programmed cell death, releasing viral PAMPs (pathogen-associated molecular patterns) and host-derived DAMPs (damage-associated molecular patterns). This release fuels a potent inflammatory response, promoting further viral reactivation and exacerbating tissue damage, creating a vicious cycle. This cycle of inflammation and reactivation contributes to both transplant-related complications and the decline of antiviral immunity in the elderly. Therefore, understanding the immune regulatory mechanisms that govern the switch from latency to reactivation is critical, especially within the unique immune landscapes of transplantation and aging. Elucidating these pathways is essential for developing strategies to prevent and treat HCMV-related disease in these high-risk populations. Full article

This paper evaluates a novel series-type suspension mount designed for electric vehicles (EVs), in which the spring and damper are arranged in series rather than in a conventional parallel configuration. This structurally simple yet innovative design avoids the need for additional mechanical components, such as inerters or costly active devices, while effectively mitigating vibration. Comparative quarter-car simulations demonstrated that the series-type configuration provided a faster reduction in transmissibility across the analyzed frequency range, highlighting its superior isolation capability compared to conventional mounts. An extended series-type model was also investigated by incorporating auxiliary sub-mount elements to assess the parametric effects. The results showed that damping variations had a limited influence, whereas the sub-mount stiffness played a decisive role in shaping the transmissibility curves and generating the secondary resonance behavior. To validate the concept experimentally, a prototype consisting of four coil springs and a vibration isolation pad was prepared and tested using impact-hammer excitation. The measured transmissibility confirmed improved vibration isolation up to 100 Hz under the given specimen conditions, with resonance features attributable to the inherent stiffness of the isolation pad. Overall, the findings verified that a simple series-type mount can provide efficient and practical vibration isolation tailored to EV applications. Full article

This paper focuses on the control problems of a multi-modal underwater robot, which is designed mainly for the task of detecting the working environment in deep-sea mining. To tackle model uncertainty and external disturbances, an improved linear active disturbance rejection control scheme based on sliding mode control is proposed (SM-ADRC). Firstly, to reduce overshoot, a piecewise $fhan$ function is introduced into the tracking differentiator (TD). This design retains the system’s fast nonlinear tracking characteristics outside the boundary layer while leveraging linear damping within it to achieve effective overshoot suppression. Secondly, two key enhancements are made to the SMC: an integral sliding surface is designed to improve steady-state accuracy, and a saturation function replaces the sign function to suppress high-frequency chattering. Furthermore, the SMC integrates the total disturbance estimate from the linear extended state observer (LESO) for feedforward compensation. Finally, the simulation experiment verification is completed. The simulation results show that the SM-ADRC scheme significantly improves the dynamic response and disturbance suppression ability of the system and simultaneously suppresses the chattering problem of SMC. Full article

The virtual synchronous generator (VSG) technique plays a crucial role in power systems with high penetration of power electronics, as it can provide virtual inertia and damping performance by emulating the swing characteristics of a synchronous generator (SG). However, the VSG faces challenges due to its inherent limitations, such as vulnerability to disturbances and instability in strong grid conditions. To address these issues, this article proposes an exchanged VSG (eVSG) control strategy. In this approach, the phase information (θ) is derived from reactive power (Q), while the voltage information (E) is derived from active power (P). Furthermore, a Magnitude-Phase Motion Equation (MPME) is introduced to analyze the eVSG system from a physical perspective. Additionally, this article is the first to illustrate the oscillation propagation effect between P and frequency (f) in both VSG and eVSG systems. Finally, the advantages of the eVSG strategy are comprehensively demonstrated through three aspects: (1) comparing the motion trajectory of f using the MPME model, (2) evaluating the oscillation propagation effect between VSG and eVSG systems, and (3) conducting simulations and experiments. Full article

Mitochondria are crucial for a wide range of cellular processes. One of the most important is innate immunity regulation. Apart from functioning as a signaling hub in immune reactions, mitochondrial nucleic acids can themselves act as damage-associated molecular patterns (DAMPs) to participate in immune processes directly. This review synthesizes the current understanding of mitochondrial RNA (mtRNA) biology and its link to immune activation through aberrant accumulation. We focus on its origin through bidirectional mitochondrial transcription and metabolism, encompassing maturation (cleavage, polyadenylation, modification) and degradation. Dysregulation of mtRNA metabolism leads to mt-dsRNA (mitochondrial double-stranded RNA) accumulation, which escapes mitochondria via specific channels into the cytosol and serves as DAMPs to trigger an immune response. We discuss the critical roles of key regulatory factors, including PNPT1 (PNPase, Polyribonucleotide Nucleotidyltrans ferase 1), in controlling mt-dsRNA levels and preventing inappropriate immune activation. Finally, we review the implications of mt-dsRNA-driven inflammation in human diseases, including autoimmune disorders, cellular senescence, and viral infection pathologies, highlighting unresolved questions regarding mt-dsRNA release mechanisms. Full article

The unsteady and discontinuous liquid flow in the microchannel affects the efficiency of sample mixing, molecular detection, target acquisition, and biochemical reaction. In this work, an active method of reducing the flow pulsation in the microchannel of a pneumatic microfluidic chip is proposed by using an on-chip membrane microvalve as a valve chamber damping hole or a valve chamber accumulator. The structure, working principle, and multi-physical model of the reducing element of reducing the flow pulsation in a microchannel are presented. When the flow pulsation in the microchannel is sinusoidal, square wave, or pulse, the simulation effect of flow pulsation reduction is given when the membrane valve has different permutations and combinations. The experimental results show that the inlet flow of the reducing element is a square wave pulsation with an amplitude of 0.1 mL/s and a period of 2 s, the outlet flow of the reducing element is assisted by 0.017 and the fluctuation frequency is accompanied by a decrease. The test data and simulation results verify the rationality of the flow reduction element in the membrane valve microchannel, the correctness of the theoretical model, and the practicability of the specific application, which provides a higher precision automatic control technology for the microfluidic chip with high integration and complex reaction function. Full article

Current ringing in dual-active bridge (DAB) converters significantly degrades efficiency and reliability, particularly due to resonant interactions in the magnetic tank impedance network. We propose a novel impedance resonant channel shaping technique to suppress the ringing by systematically modifying the converter’s equivalent impedance model. The method begins with establishing a high-fidelity network representation of the magnetic tank, incorporating transformer parasitics, external inductors, and distributed capacitances, where secondary-side components are referred to the primary via the turns ratio squared. Critical damping is achieved through a rank-one modification of the coupling denominator, which is analytically normalized to a second-order form with explicit expressions for resonant frequency and damping ratio. The optimal series–RC damping network parameters are derived as functions of leakage inductance and winding capacitance, enabling precise control over the effective damping factor while accounting for core loss effects. Furthermore, the integrated network with the damping network dynamically shapes the impedance response, thereby attenuating ringing currents without compromising converter dynamics. Experimental validation confirms that the proposed approach reduces peak ringing amplitude by over 60% compared to the conventional snubber-based methods, while maintaining full soft-switching capability. Full article

Traditional anti-seismic methods are constrained by high construction costs and the potential for severe structural damage under earthquakes. Energy dissipation technology provides an effective solution for structural earthquake resistance by incorporating energy-dissipating devices within structures to actively absorb seismic energy. However, existing research lacks in-depth analysis of the influence of energy dissipation devices’ placement on structural dynamic response. Therefore, this study investigates the seismic mitigation effectiveness of viscous dampers in multi-storey frame structures and their optimal placement strategies. A comprehensive parametric investigation was conducted using a representative three-storey steel-frame kindergarten facility in Shandong Province as the prototype structure. Advanced finite element modeling was implemented through ETABS software to establish a high-fidelity structural analysis framework. Based on the supplemental virtual damping ratio seismic design method, damping schemes were designed, and the influence patterns of different viscous damper arrangement schemes on the seismic mitigation effectiveness of multi-storey frame structures were systematically investigated. Through rigorous comparative assessment of dynamic response characteristics and energy dissipation mechanisms inherent to three distinct energy dissipation device deployment strategies (perimeter distribution, central concentration, and upper-storey localization), this investigation delineates the governing principles underlying spatial positioning effects on structural seismic mitigation performance. This comprehensive investigation elucidates several pivotal findings: damping schemes developed through the supplemental virtual damping ratio-based design methodology demonstrate excellent applicability and predictive accuracy. All three spatial configurations effectively attenuate structural seismic response, achieving storey shear reductions of 15–30% and inter-storey drift reductions of 19–28%. Damper spatial positioning critically influences mitigation performance, with perimeter distribution outperforming central concentration, while upper-storey localization exhibits optimal overall effectiveness. These findings validate the engineering viability and structural reliability of viscous dampers in multi-storey frame applications, establishing a robust scientific foundation for energy dissipation technology implementation in seismic design practice. Full article

Deep hole structures are widely used in the fields of aerospace, engineering machinery, marine, etc. During the deep hole machining processes, especially for boring procedures, the vibration phenomenon caused by the large aspect ratio of boring tools seriously restricts the machining accuracy and production efficiency. Therefore, extensive research has been devoted to the design and development of damped boring tools with different structures to suppress machining vibration. According to varied vibration reduction technologies, the damped boring tools can be divided into active and passive categories. This paper systematically reviews the advancements of vibration reduction principles, structure design, and practical applications of typical active and passive damped boring tools. Active damped boring tools rely on the synergistic action of sensors, actuators, and control systems, which can monitor vibration signals in real-time during the machining process and achieve dynamic vibration suppression through feedback adjustment. Their advantages include strong adaptability and wide adjustment capability for different machining conditions, including precision machining scenarios. Comparatively, vibration-absorbing units, such as mass dampers and viscoelastic materials, are integrated into the boring bars for passive damped tools, while an energy dissipation mechanism is utilized with the aid of boring tool structures to suppress vibration. Their advantages include simple structure, low manufacturing cost, and independence from an external energy supply. Furthermore, the potential development directions of vibration damped boring bars are discussed. With the development of intelligent manufacturing technologies, the multifunctional integration of damped boring tools has become a research hotspot. Future research will focus more on the development of an intelligent boring tool system to further improve the processing efficiency of deep hole structures with difficult-to-machine materials. Full article

We report high-resolution, cavity-enhanced direct frequency comb Fourier transform spectroscopy of cold acetylene (C₂H₂) molecules in a planar supersonic jet expansion. The experiment is based on a near-infrared frequency comb with a 300 MHz effective repetition rate, matched to a high-finesse enhancement cavity traversing the jet. The rotational and translational cooling of acetylene was achieved via expansion in argon carrier gas through a slit nozzle. By interleaving successive mode-resolved spectra measured at different comb repetition rates, we retrieved full absorption line profiles. Spectroscopic analysis reveals sharp, Doppler-limited transitions corresponding to a jet core rotational temperature below 7 K. Frequency comb and cavity stabilization were achieved through active Pound–Drever–Hall locking and mechanical vibration damping, enabling a spectral precision better than 2 MHz, limited by the vibrations induced by the pumping system. The demonstrated sensitivity reaches a minimum detectable absorption of 7.8 × 10⁻⁷ cm⁻¹ over an 18 m effective path length in the jet core. This work illustrates the potential of cavity-enhanced direct frequency comb spectroscopy for precise spectroscopic characterization of cold supersonic expansions, with implications for studies in molecular dynamics, reaction kinetics, and laboratory astrophysics. Full article

Due to the “low damping” characteristics of the DC distribution system, the traditional passive scheme is not suitable for DC fault detection and positioning. Therefore, this paper proposes an active injection fault identification method suitable for DC feeder line under single-pole grounding faults. Based on the high controllability of converters, this method uses the oscillation circuit characteristics of the DC side single-pole grounding fault to superimpose the harmonics of fixed frequency into the converter modulated wave, and derives the selection principles of harmonic amplitude and frequency. After the fault, the positive and negative current signals are extracted from the feeder lines, and the zero-mode current components are extracted by the Karrenbauer transformation and band-pass filter, the current phases are compared to achieve the fault feeder line selection. According to simulation verification, the power quality of the actively injected harmonics is within the standard range under the condition of global injection, and the single-pole grounding faults in each feeder line can be identified. Full article