Search Results (8,755)

This study examines the early impact of Industry 4.0 (I4.0) implementation on the financial performance of Croatian companies, focusing on indicators of profitability, efficiency, and activity. The research investigates whether firms adopting I4.0 technologies achieve superior results compared to traditional companies. A unique feature of this study is its integration of primary data—collected via an online survey of Croatian enterprises—with secondary data from publicly available financial reports. Statistical methods, including Analysis of Variance (ANOVA) and linear regression, were employed to test the hypotheses. The results show that I4.0 adopters perform significantly better in terms of net profit margin, return on assets, business efficiency, and supplier bonding days, while no significant difference was found in days sales outstanding. This paper contributes to the literature by offering one of the first empirical analyses of early-stage I4.0 adoption in the context of a transition economy, using firm-level financial data. The findings provide valuable insights for managers, policymakers, and investors aiming to understand the tangible business benefits of digital transformation. The results also highlight the importance of supporting I4.0 adoption strategies to enhance competitiveness and recovery in post-pandemic economic conditions. Full article

The fungal genus Trichoderma is highly valued in agriculture for its versatile roles, mainly as a biocontrol agent against plant pathogens. Recently, its use as a natural biofertilizer has gained attention, as Trichoderma spp. promotes crop growth and improves yield by enhancing the rhizosphere environment and activating plant defences. Globally, over 250 Trichoderma-based products dominate 60–90% of the market, but their efficacy can decline during transportation and storage. Additionally, concerns about their impact on native soil biodiversity have led to interest in using locally adapted, native strains. The novel native strain of Trichoderma sp. STP8 (formerly T. koningiopsis agg. STP8) previously showed strong antagonism against Sclerotinia sclerotiorum and promoted lettuce growth in greenhouse conditions. This study evaluated Trichoderma sp. STP8’s effectiveness in field-grown lettuce, revealing yield increases of 16.6% to 30.5%. The most significant gains occurred when Trichoderma sp. STP8 was applied before head formation, 26 days after planting. That was in one treatment with two applications (at seedling planting and after 26 days) and another with three applications (at sowing, at seedling planting, and after 26 days). These results demonstrate Trichoderma sp. STP8’s potential as a sustainable biocontrol and biofertilizer agent for lettuce, encouraging further research across different agricultural systems, including hydroponics and soil-less medium. Full article

Vibrio sp. is one of the main producers of alginate lyase; however, most strains have problems such as low and unstable enzyme production. In this study, the enzyme production conditions of V. sp. 32415, a marine bacterium capable of producing extracellular alginate lyase, were optimized through Response Surface Design. The optimized medium was as follows: NaCl 12 g/L, FeSO₄·7H₂O 0.067 g/L, NH₄Cl 7 g/L, alginate 11 g/L, K₂HPO₄·3H₂O 4 g/L, MgSO₄·7H₂O 1 g/L. Under 28 °C, 160 rpm, 30 mL/300 mL liquid volume, and an initial pH 5.5 culture condition, the extracellular enzyme activity was 51.06 U/mL, which was 2.8 times higher compared with the activity before optimization. The optimal temperature, pH, and NaCl concentration for the extracellular alginate lyase were 37 °C, 8.0, and 0.1 M, respectively. The enzyme remained more than 80% of its original activity at 30 °C for 4 h. 1 mM Fe³⁺, Ca²⁺, K⁺, Mg²⁺, and Na⁺ enhance enzyme activity, with a preference for polyG blocks. V. sp. 32415 has two circular chromosomes and one circular plasmid. Chromosome 2 has two polysaccharide utilization loci. It utilizes alginate through the Scatter pathway. The results of this study provide theoretical and data support for understanding the production of extracellular alginate lyase by marine Vibrio and their metabolism and utilization of alginate. Full article

In a competitive global economy, the efficient use of human capital is a key determinant of productivity, growth, and sustainable development. This study assesses the efficiency of human capital in the Mexican manufacturing sector, with a focus on three strategic subsectors: the chemical industry, the food industry, and the transport equipment industry. The purpose is to analyze how human capital—measured through training, average wages, and daily working hours—relates to firm performance across different branches of economic activity and company sizes. Firm-level data from the National Institute of Statistics and Geography (INEGI) for the period 2009–2021 are analyzed using an input-oriented Data Envelopment Analysis (DEA) with CCR and BCC models. The results reveal significant differences in human capital efficiency across branches of economic activity within each—micro, small, and medium and large—firm size. Overall, the results highlight the central role of human capital investment in enhancing firm competitiveness and advancing the sustainable development of strategic industries. Policy implications underscore the need for training and wage strategies that improve efficiency and strengthen the long-term resilience of the Mexican manufacturing sector. Full article

The evaluation of sperm proteins has emerged as a promising approach to predicting semen quality across animal species. This study investigated the relationship between post-thaw concentrations of precursor A-kinase anchor protein 4 (proAKAP4) and objective sperm quality parameters in goats. Semen was collected from 16 adult goats (Boer, n = 8; Anglo-Nubian, n = 8) and frozen using a standardized protocol with OptiXcell (IMV Technologies, l′Aigle, France) extender (n = 5). After thawing, proAKAP4 concentrations were measured with an enzyme-linked immunosorbent assay (ELISA), while sperm motility and kinematics were assessed with computer-assisted analysis (CASA), and viability, plasma membrane integrity, acrosome integrity, and mitochondrial activity were evaluated using flow cytometry. Samples were grouped according to low, medium, or high proAKAP4 levels for comparison, and correlations with sperm parameters were examined. The results showed that semen with higher proAKAP4 concentrations had significantly greater total and progressive motility, more favorable kinematic values, and improved viability, plasma membrane integrity, and mitochondrial function (p < 0.05), whereas acrosome integrity was not influenced (p > 0.05). The average post-thaw proAKAP4 concentration was 38.66 ± 1.11 ng/10⁶ sperm, and no differences were observed between Boer and Anglo-Nubian breeds (p > 0.05). These findings indicate that proAKAP4 is strongly associated with multiple sperm functional traits and may serve as a reliable biomarker for assessing post-thaw semen quality in goats. Full article

Background/Objectives: The growing threat of antimicrobial resistance has intensified the search for alternative therapeutic approaches. Quorum sensing (QS) inhibition, which disrupts bacterial communication and virulence, represents a promising approach to mitigating infection. Given the complexity of the sponge holobiont, sponge-associated microorganisms may demonstrate QS inhibitory properties and serve as potential sources of novel anti-virulence agents. This study aimed to investigate the QS inhibitory potential of sponge-associated Bacillus species against Pseudomonas aeruginosa, a multidrug-resistant pathogen that relies on QS for virulence regulation. Methods: Ninety-eight bacterial isolates were obtained from seven intertidal South African sponges. Biosensor-based sandwich assays using Chromobacterium violaceum identified 15 isolates with putative QS inhibition (QSI) activity, including five classified as Bacillus species via 16S rRNA gene sequencing. Crude extracts from these isolates, cultivated in medium Mannitol (Mann) and medium 5294, were screened for their ability to inhibit QS-regulated virulence factors in P. aeruginosa. Results: Extracts, particularly from medium 5294, exhibited significant QSI activity without cytotoxic effects. The five most potent extracts, i.e., Bacillus mobilis SP2-AB7 (5294), Bacillus wiedmannii SP5-AB7 (Mann), B. mobilis SP2-AB7 (Mann), and Bacillus cereus SP1-AB4 (Mann and 5294), inhibited both Las- and Rhl-regulated virulence factors, including pyocyanin, pyoverdine, elastase, protease, rhamnolipid production, motility, and initial adhesion, achieving inhibition rates of up to 93% (p < 0.05). Molecular analysis confirmed the presence of the aiiA lactonase gene in key isolates, while GC-MS and FTIR profiling revealed medium-specific differences in metabolite production. Conclusions: Sponge-associated Bacillus species from KwaZulu-Natal exhibit robust QSI activity against P. aeruginosa, highlighting their potential as sources of alternative anti-virulence agents. Further characterization and in vivo validation are needed to assess their therapeutic application in combatting resistant infections. Full article

Coffee is among the most consumed beverages worldwide and is recognized for its bioactive compounds, which exert diverse physiological effects. This study evaluated the impact of roasting degree on the in vitro antioxidant activity and digestive enzyme inhibition of brews from four Mexican regions, as well as their in vivo effects on carbohydrate and lipid absorption. Antioxidant capacity was assessed through radical scavenging and lipid peroxidation inhibition, while inhibition of lipase, α-amylase, and α-glucosidase was also determined. Oral starch (OSTT) and lipid (OLTT) tolerance tests were conducted in healthy Wistar rats. Antioxidant activity was strongly influenced by region and roasting degree. All coffee samples exhibited radical scavenging activity and lipid peroxidation inhibition. With respect to enzyme inhibition, all coffees showed ~67–70% inhibition of lipase activity. For amylase, unroasted coffee from Oaxaca displayed the highest inhibition (34%, p < 0.001). For glucosidase, unroasted samples showed low inhibition (~6–19%), which increased substantially at the medium roast degree (~55% across all samples) but decreased again at the high roast degree (~27%). In OSTT, serum glucose levels were reduced after 120 min by ~20%, 21%, and 18% in rats treated with unroasted, medium-roast, and high-roast coffee, respectively, compared with the negative control. In OLTT, serum triglycerides decreased by ~26% (Chiapas), ~58% (Colima), ~32% (Oaxaca), and ~54% (Hidalgo). Crop region and roasting degree influence the phytochemical profile and bioactivity of Mexican coffee. Although unroasted coffees had the highest concentration of bioactive compounds, roasting enhanced specific bioactivities, particularly enzyme inhibition and lipid-lowering effects in vivo. Full article

Electrospun fibers serve as a medium for the targeted release of active compounds, facilitating the desired therapeutic effects in drug administration. The point of this study was to find the best conditions for making electrospun fibers from cellulose acetate (CA) and poly(ε-caprolactone) (PCL), mixed with pure oxyresveratrol extract from Artrocarpus lakoocha Roxberg (Moraceae). Additionally, the study focused on evaluating the antioxidant properties, antityrosinase activity, and freeze–thaw stability of the resulting fibers. We incorporated a concentration of oxyresveratrol at 0.1% w/w into various mass ratios of CA/PCL blended fiber sheets (1:0, 3:1, 1:1, 1:3), utilizing mixed solvents of acetone/DMF (2:1% v/v) and chloroform/DMF (9:1% v/v) for preparation. The fiber sheets displayed a continuous and uniform structure, with fiber diameters ranging from 300 to 1000 nanometers. We investigated the release kinetics of oxyresveratrol from the fibrous substrates using the total immersion technique, specifically in phosphate-buffered saline at a pH of 7.4. The results showed that the fiber sheet with a 3:1 w/w ratio of CA to PCL and a 0.1 w/w loading of oxyresveratrol showed the most significant release of oxyresveratrol at the 2 h mark, and it continued to release consistently at this peak value for up to 24 h. The antioxidant and anti-tyrosinase properties of oxyresveratrol in fiber sheets were more stable than those of free oxyresveratrol at the same concentrations. The fiber sheet presents a promising avenue for a user-friendly transdermal patch application. Full article

Microbial coal gasification technology is a new, efficient, and clean method for coal resource mining; however, its commercial application remains limited by gasification efficiency and cost. To examine the impact of biomass on microbial coal gasification processes, improve gas production efficiency, and identify economically viable nutrient supplements with broad availability, this study employed culture medium (CM), sawdust (SD), and blue-green algae (BA) as nutritional additives. An anaerobic fermentation experiment with three-stage nutrient supplementation was conducted using a fed-batch reactor. The comparison of gas and liquid compositions within reactors throughout the reaction process demonstrated that key nutrient supplementation could reactivate methane production in reactors in which gas generation had ceased. Gas composition analysis revealed that under identical conditions, BA supplementation achieved the highest methane yield (24.49 ± 1.31 mL/g), followed by SD (1.56 ± 0.1 mL), representing 24.13-fold and 1.53-fold increases, respectively, compared with the yield in CM control group. Analysis of 16S rRNA sequencing indicated that nutrient supplementation induced microbial community differentiation, with dominant bacterial genera (Herbinix, Proteiniborus) shifting according to the organic composition of the liquid environment. A positive feedback relationship between microbial life activities and functional performance further confirmed the dominance of these superior strains. This study advances the understanding of substrate degradation characteristics in microbial coal degradation systems and provides theoretical support for the clean and efficient coal exploitation. Full article

Objectives: The growing proportion of older adults underscores the importance of healthy aging. Maintaining good nutrition and physical activity are crucial for sustaining health. However, research on aging inequalities suggests that individuals with lower socioeconomic status (SES) may be at higher risk for inadequate nutrition. The study examined associations between SES and nutrition in older community-dwelling adults in Iceland and whether the Sustainable Development Goals (SDGs) are being met in Iceland. Methods: Data from the AGES-Reykjavik study were used, and SES was grouped into four categories (low, medium-low, medium-high, and high), derived from education and occupation. Descriptive statistics were used to examine differences between groups, and logistic regression was used to explore associations with food and drink consumption. Results: Older adults with low SES were less likely to frequently consume vegetables, fruit, cod or saithe liver oil/pills, oatmeal or muesli, and cultured milk products, and they were less likely to meet nutritional guidelines. Higher SES was associated with greater alcohol intake. Conclusions: Older adults with low socioeconomic status are less likely to consume healthy food products than those with higher SES. As these individuals live independently, the findings highlight the need for targeted nutritional prevention and support to reduce health disparities, including potential risks such as insufficient vitamin D intake. Furthermore, the results suggest that the SDGs related to nutrition are not being met in Iceland, warranting monitoring and policy action. Full article

The structural evolution of concrete during different hydration stages critically influences subsequent strength, and continuous monitoring throughout this process has become a research focus in materials science. This study proposes an embedded ultrasonic active sensing technique based on piezoelectric ceramics (PZT) to identify key structural transition stages during concrete curing. To this end, a piezoelectric ultrasonic sensor was fabricated and its comprehensive performance was systematically evaluated. Subsequently, compressive strength and penetration resistance tests were conducted, and the evolution of piezoelectric signal amplitude and wavelet packet energy (WPE) during hydration was analyzed. Furthermore, a root mean square deviation index based on WPE (WPE-RMSD) was introduced to identify structural transitions throughout the hydration process. The results demonstrate that the developed sensor exhibits stable electrical, mechanical, and waterproof performance. Both signal amplitude and WPE effectively captured the hydration process of concrete, with WPE showing higher sensitivity. The WPE-RMSD index exhibited good temporal continuity, covering the entire process from early hydration disturbance to late-stage structural densification (28 d), and proved particularly effective in identifying critical stages such as final setting and the medium-age period (7 d). This study provides a novel in situ monitoring approach for the classification and identification of hydration stages in concrete. Full article

This study aims to develop and characterize innovative geopolymer composites by incorporating activated carbon into a geopolymer matrix to create a novel, effective sorption material suitable for non-dusty or medium-temperature environmental applications. Specifically, it examines the impact of using a single source of activated carbon, both in its original granular form and milled form, at two different loading levels for each. The research focuses on evaluating how these variations influence the textural, adsorption, mechanical, and thermal properties of the resulting geopolymer composites, with particular attention to strength and thermal stability under operational conditions. The CO₂ adsorption capacity of the composites measured at 25 °C and pressure up to 0.1 MPa varied from 48.8 to 60.0 mg.g⁻¹, with the highest performance observed at a lower content of the granular form, while commercial pure activated carbon reached 120.8 mg.g⁻¹. However, incorporation of a granular form negatively affected thermal stability (approximately 20 wt.% weight loss) and significantly reduced compressive strength (below 45 MPa) due to increased material inhomogeneity. Despite these limitations, both types of composites show promising potential for environmental applications. However, further optimization is required to balance sorption capacity, strength, and thermal stability. Full article

The present study investigated the impact of genetic engineering strategies to produce a cell-free xylanase for applications in the baking industry. The xynA1 gene from the nonpathogenic bacterium Caulobacter vibrioides was integrated into the pAS22 vector with a xylose-inducible promoter and introduced back into the bacteria, resulting in the creation of the BS-xynA1. This construct exhibited substantial secreted xylanase 1 (XynA1) activity, reaching 17.22 U/mL, and a specific activity of 278.64 U/mg after an 18 h growth period with 0.3% (v/v) xylose plus 0.2% (w/v) corn straw. RT-qPCR analysis confirmed that higher xylanase activity in C. vibrioides cells was correlated with increased transcription of the xynA1 gene in the induction medium. Moreover, BS-xynA1 cells coexpress other enzymes, including xylanase 2 (XynA2), cellulase, pectinase, α-amylase, β-glucosidase, β-xylosidase, and α-L-arabinosidase, at low levels (≤2 U/mL). In vitro comparison of cell-free xylanases from BS-xynA1 with three commercially available xylanase-containing mixtures commonly utilized in baking protocols revealed its superior specific activity (163.4 U/mg) across a broad temperature range (30–100 °C), with optimal performance at 50 °C. In practical baking tests, the addition of cell-free XynA1 led to a reduction in dough kneading time and increase in bread height compared to those of the control. Notably, the incorporation of XynA1 resulted in enhanced alveolar structure formation within the bread crumb. Specifically, the following changes were observed in the mass parameters compared to those of the control: an increase in extensibility, elasticity, and deformation energy, and subsequent improvements in strength. Additionally, XynA1 addition led to a reduction in toughness and toughness/elasticity index, indicating a reduction in the mass stiffness of the enzyme-treated bread. To date, this is the first successful application of recombinant XynA1 from C. vibrioides in biotechnological processes related to baking, underscoring the potential and prospects in the food industry. Full article

This study examined the diffusion of nutrients (NH₄⁺, NO₃⁻, and PO₄³⁻) in the pore water of intertidal sediments in Lake Komuke (located in the northeastern part of Hokkaido on the coast of Okhotsk Sea) across nine stations, at 0 cm to 40 cm depths, from 19 May to 23 August 2015. A comparison was made between the traditional version of Fick’s first law and a modified version to understand nutrient diffusion in the intertidal flat. The novelty of this study lies in establishing a flux correction factor as a function of the activity coefficient in intertidal sediments. The outcomes of the standard and modified versions of Fick’s law exhibited observable physical differences between stations for all nutrients. However, the statistical analysis demonstrated significant differences only for NO₃⁻ and PO₄³⁻ at stations D, E, and F, suggesting that there is no statistically meaningful difference in NH₄⁺ flux. A statistical comparison of 27 pairs of fluxes obtained by the classical and modified versions of Fick’s first law indicated that 88.9% of the differences were not statistically significant between the two methods, and 11.1% were statistically significant. This shows the consistency of the modified method, firstly in reproducing the classical version of Fick’s law, and secondly in improving upon it, in a porous medium. These findings indicate that the modified version of Fick’s first law is an accurate methodology for evaluating nutrient diffusion dynamics in intertidal environments, and is potentially applicable to coastal salt marshes, with practical implications for environmental management and conservation efforts. Full article

The synergistic combination of bismuth and its compounds with the exceptional properties of single-walled carbon nanotubes (SWCNT) was investigated as a sensing platform for the sensitive detection of gallic acid and as a standard for the determination of total phenol. Four bismuth-based electrodes were used for this purpose: SWCNT with bismuth (SWCNT/Bi) or bismuth (III) oxide (SWCNT/Bi₂O₃) and bismuth or bismuth (III) oxide electrodeposited on a glassy carbon electrode (ELF/Bi and ELF/Bi₂O₃), which were morphologically characterized by scanning electron microscopy. Cyclic voltammetry in phosphate electrolyte at different pH values revealed that the SWCNT/Bi₂O₃ electrode exhibited optimal performance for the analytical determination of gallic acid at pH 3. Surface-active carbon nanotubes facilitate the adsorption and accumulation of gallic acid, while the addition of Bi₂O₃ improves electron transfer, resulting in a synergistic enhancement of the oxidation signal. Square-wave voltammetry with SWCNT/Bi₂O₃ electrodes also provided reliable and accurate results and proved to be suitable for the quantitative determination of gallic acid with wide linearity (0.2–80 µM) and sensitivities of 12.5, 2.35, and 0.385 µA µmol⁻¹ dm³ for low, medium, and high concentration ranges, respectively. The limit of detection was 0.06 µmol dm⁻³. Finally, the electrode was successfully applied for gallic acid determination in various seeds. Full article