Search Results (113)

Despite the abundance of available studies on optical and microwave methods devoted to investigating agricultural crop conditions, there is a lack of research that explores the integration between microwave and optical data and the link between photosynthetic activity, measured by PRI (photochemical reflectance index), and vegetation water content, detected by radar sensors. In particular, there is a lack of vision that links these measures to better understand how plants react and adapt to possible water stress conditions. Most of the existing research tends to treat optical and microwave information separately, without investigating how the integration of these techniques can provide a more complete and accurate understanding of the research topic, corroborated by ground data. In this paper, an integrated approach using microwave and optical satellite data, respectively acquired by Sentinel-1 (S-1) and Sentinel-2 (S-2), was presented for monitoring vegetation status. Experimental data and electromagnetic models have been combined to relate backscattering from S-1 and optical indices from S-2 to plant conditions, which were evaluated by measuring PRI, plant water content (PWC), and soil water content. Field data were collected in two sorghum fields close to Florence in Tuscany (Central Italy) during the summers of 2022 and 2023. The results show significant correlations between microwave and optical data with respect to field measurements, highlighting the potential of remote sensing techniques for agricultural monitoring and management, also in response to climate change. Determination coefficients of R² = 0.51 between PRI and PWC, where PWC is retrieved by S-1, and R² = 0.73 between PSRI (plant senescence reflectance index) and PRI were obtained. Full article

Sorghum is an essential crop for biofuel. Many glycosyltransferase (GT) families, including GT47, are involved in the production of both types of polysaccharides. However, a comprehensive study related to the GT47 gene family is needed. The glycosyltransferase (GT) 47 family helps in the synthesis of xylose, pectin, and xyloglucan and plays an essential role in the formation of the proper shape of the plant cell wall. In this study, we performed identification, phylogenetic tree, physiochemical properties, subcellular localization, protein–protein interaction network, detection of motif analysis, gene structure, secondary structure, functional domain, gene duplication, Cis-acting elements, sequence logos, and gene expression profiles based on RNA-sequence analyses in the GT47 gene family. As a result, we identified thirty-one members of the GT47 gene family. The phylogenetic analysis grouped them into three distinct clusters. According to their physiochemical properties, all GT47 proteins were hydrophilic, and their molecular weights ranged from 22.7 to 88.6 kDa. Three essential motifs were identified via motif and conserved domain analysis, emphasizing structural conservation. Subcellular localization was proposed for the various functional roles across cellular compartments. While gene structure analysis showed significant variation in introns–exons, promoter study verified susceptibility to phytohormones like ABA. RNA sequencing revealed that several GT47 genes were highly expressed in internodes, and this was linked to biomass accumulation, cell wall manufacturing, and stem elongation. Analysis of networks of protein–protein interactions and Cis-elements confirmed involvement in stress adaptation and growth regulation. These results contribute to a better understanding of the functional and evolutionary significance of the GT47 gene family in sorghum. Full article

The Mediterranean region faces intensified climate change effects, increasing irrigation demands to sustain crop yields and increasing pressure on water resources. Adaptive management strategies such as conservation agriculture (CA) offer potential benefits for soil quality and water use efficiency. However, there is limited research on the short-term effects of this farming system under irrigated Mediterranean climatic conditions. This study aimed to explore the short-term impacts of conservation agriculture (no tillage, cover crops and crop rotation) on the soil properties, water flows and crop and water productivity in a French Mediterranean agrosystem of irrigated field crops, using a multifactorial approach. From 2021 to 2023, maize, sorghum and soybean were grown successively under either conventional tillage (CT) or conservation agriculture (CA), combined with sprinkler irrigation, subsurface drip irrigation or non-irrigated conditions. The dynamics of the surface soil properties (bulk density, penetration resistance, soil temperature), water flows (infiltration, soil evaporation) and agronomic indicators (leaf area index, crop yield, water productivity) were measured across the three cropping seasons. In the pedoclimatic conditions of the study, CA was shown to clearly impact the soil properties, water flows and crop yields, from the first year of adoption. CA practices caused an increased bulk density and soil resistance penetration, leading to decreased quasi-steady ponded infiltration in the surface horizon, particularly in the CA–subsurface drip and CA–non-irrigated conditions. These effects were also reflected in the leaf area index, crop yield and water productivity, with CA showing lower values compared to CT. Crop residues in CA reduced soil evaporation, particularly under sprinkler irrigation. However, this benefit diminished as the residues decomposed, leading to soil evaporation rates comparable to those observed in CT. Agronomic indicators were better under sprinkler irrigation than under subsurface drip irrigation. Overall, compaction emerged as a significant challenge in the adoption of CA, considering its negative impact on crop yields. Full article

Semi-arid regions present edaphoclimatic limitations for forage production, primarily affecting plant growth and development. Crops adapted to such conditions, like forage sorghum, and nutritional supplementation with nitrogen and molybdenum, can increase forage production. The objective of this study was to evaluate the interaction between nitrogen and molybdenum on the bromatological and structural components of forage sorghum (SF-15) cultivated in a semi-arid environment, with the hypothesis that nitrogen fertilization combined with molybdenum would enhance nitrogen use efficiency in sorghum. The methodology involved a 5 × 2 factorial experiment in a randomized block design (RBD) with increasing doses of nitrogen (urea) (0, 50, 100, 150, 300 kg ha⁻¹) and two doses of molybdenum (sodium molybdate): 0 and 160 g ha⁻¹, conducted over three cultivation cycles. At the end of each cycle, morphological variables were evaluated, and yield of natural mass (YNM), yield of dry mass (YDM), crude protein production (CPP), and bromatological components were determined. Morphometric characteristics were influenced by the interaction between cycle x nitrogen doses (N) and molybdenum doses (Mo). For productive characteristics, there was an interaction between cycle and nitrogen doses, with the first regrowth cycle and the dosage of 100 kg ha⁻¹ N showing the highest mean. Bromatological components were influenced by the N and Mo interaction. The study confirmed the synergistic effect between nitrogen and molybdenum. It is recommended to use 100 kg ha⁻¹ nitrogen fertilization for an average production of 10 t ha⁻¹ for SF-15 sorghum. Full article

Fumonisins, a class of mycotoxins predominantly produced by Fusarium species, represent a major threat to food safety and public health due to their widespread occurrence in staple crops including peanuts, wine, rice, sorghum, and mainly in maize and maize-based food and feed products. Although fumonisins occur in different groups, the fumonisin B series, particularly fumonisin B1 (FB1) and fumonisin B2 (FB2), are the most prevalent and toxic in this group of mycotoxins and are of public health significance due to the many debilitating human and animal diseases and mycotoxicosis they cause and their classification as by the International Agency for Research on Cancer (IARC) as a class 2B carcinogen (probable human carcinogen). This has made them one of the most regulated mycotoxins, with stringent regulatory limits on their levels in food and feeds destined for human and animal consumption, especially maize and maize-based products. Numerous countries have regulations on levels of fumonisins in foods and feeds that are intended to protect human and animal health. However, there are still gaps in knowledge, especially with regards to the molecular mechanisms underlying fumonisin-induced toxicity and their full impact on human health. Detection of fumonisins has been advanced through various methods, with immunological approaches such as Enzyme-Linked Immuno-Sorbent Assay (ELISA) and lateral flow immunoassays being widely used for their simplicity and adaptability. However, these methods face challenges such as cross-reactivity and matrix interference, necessitating the need for continued development of more sensitive and specific detection techniques. Chromatographic methods, including HPLC-FLD, are also employed in fumonisin analysis but require meticulous sample preparation and derivitization due to the low UV absorbance of fumonisins. This review provides a comprehensive overview of the fumonisin family, focusing on their biosynthesis, occurrence, toxicological effects, and levels of contamination found in foods and the factors affecting their presence. It also critically evaluates the current methods for fumonisin detection and quantification, including chromatographic techniques and immunological approaches such as ELISA and lateral flow immunoassays, highlighting the challenges associated with fumonisin detection in complex food matrices and emphasizing the need for more sensitive, rapid, and cost-effective detection methods. Full article

Sorghum is a climate-resilient crop which has been cultivated as a staple food in the semi-arid areas of Africa and Asia for food and nutrition security. However, the current climate change is increasingly affecting sorghum performance, especially at the flowering stage when water availability is critical for grain filling, thus lowering the sorghum grain yield. The development of climate-resilient, biotic and abiotic stress-tolerant, market-preferred, and nutrient-dense sorghum varieties offers a potentially cost-effective and environmentally sustainable strategy for adapting to climate change. Some of the common technologies for sorghum improvement include mass selection, single seed descent, pure line selection, and marker-assisted selection, facilitated by backcrossing and genotyping using molecular markers. In addition, recent advancements including new machine learning algorithms, gene editing, genomic selection, rapid generation advancement, and recycling of elite material, along with high-throughput phenotyping tools such as drone- and satellite-based images and other speed-breeding techniques, have increased the precision, speed, and accuracy of new crop variety development. In addition to these modern breeding tools and technologies, enhancing genetic diversity to incorporate various climate resilience traits, including against heat and drought stress, into the current sorghum breeding pools is critical. This review covers the potential of sorghum as a staple food crop, explores the genetic diversity of sorghum, discusses the challenges facing sorghum breeding, highlights the recent advancements in technologies for sorghum breeding, and addresses the perceptions of farmers on sorghum production under the current climate change conditions. Full article

The study aimed to evaluate the genetic diversity of 41 sweet sorghum genotypes from seven countries for key biofuel-related traits over two sorghum growing seasons (2020 and 2021) in a semi-arid Mediterranean environment. Genotypes were assessed for key bioethanol production traits such as fresh stalk yield (FSY), juice yield (JY), Brix, dry biomass yield (DBY), theoretical juice ethanol yield (JEY), theoretical lignocellulosic ethanol yield (LEY), theoretical total ethanol yield (TEY), as well as their potential for solid biofuel production, measured through bagasse ash, nitrogen, and moisture content. Significant variations were observed among genotypes across most traits, analyzed using analysis of variance, principal component analysis, and hierarchical cluster analysis, identifying DBY, LEY, and TEY as the primary contributors to overall genetic variation. Cluster analysis grouped the genotypes into five distinct clusters, highlighting the diversity in biofuel-related traits. The correlation analysis revealed significant positive relationships between traits such as FSY, DBY, and TEY, indicating that higher biomass production directly enhances ethanol output. Strong positive correlations between FSY and TEY underscore the importance of biomass yield in maximizing ethanol production. However, traits like bagasse ash and nitrogen contents, which were negatively correlated with JEY, LEY, and TEY, present challenges in improving biomass combustion quality. The study concludes that certain genotypes, particularly ‘Yellow Bonnet’ and ‘IS 3556’, exhibited strong potential for biofuel production in semi-arid Mediterranean climates where water scarcity and high temperature favor genotypes with high biomass and sugar yields. These genotypes demonstrated high JEYs, LEYs, and TEYs, suggesting their adaptability to the unique environmental stresses of this region, making them good candidates for sustainable biofuel production in similar climates. Full article

Carotenoid cleavage oxygenases (CCOs) cleave carotenoid molecules to produce bioactive products that influence the synthesis of hormones such as abscisic acid (ABA) and strigolactones (SL), which regulate plant growth, development, and stress adaptation. Here, to explore the molecular characteristics of all members of the OsCCO family in rice, fourteen OsCCO family genes were identified in the genome-wide study. The results revealed that the OsCCO family included one OsNCED and four OsCCD subfamilies. The OsCCO family was phylogenetically close to members of the maize ZmCCO family and the Sorghum SbCCO family. A collinearity relationship was observed between OsNCED3 and OsNCED5 in rice, as well as OsCCD7 and OsNCED5 between rice and Arabidopsis, Sorghum, and maize. OsCCD4a and OsCCD7 were the key members in the protein interaction network of the OsCCO family, which was involved in the catabolic processes of carotenoids and terpenoid compounds. miRNAs targeting OsCCO family members were mostly involved in the abiotic stress response, and RNA-seq data further confirmed the molecular properties of OsCCO family genes in response to abiotic stress and hormone induction. qRT-PCR analysis showed the differential expression patterns of OsCCO members across various rice organs. Notably, OsCCD1 showed relatively high expression levels in all organs except for ripening seeds and endosperm. OsNCED2a, OsNCED3, OsCCD1, OsCCD4a, OsCCD7, OsCCD8a, and OsCCD8e were potentially involved in plant growth and differentiation. Meanwhile, OsNCED2a, OsNCED2b, OsNCED5, OsCCD8b, and OsCCD8d were associated with reproductive organ development, flowering, and seed formation. OsNCED3, OsCCD4b, OsCCD4c, OsCCD8b, and OsCCD8c were related to assimilate transport and seed maturation. These findings provide a theoretical basis for further functional analysis of the OsCCO family. Full article

Water scarcity is a major environmental constraint on plant growth in arid regions. Soluble sugars and amino acids are essential osmolytes for plants to cope with osmotic stresses. Sweet sorghum is an important bioenergy crop and forage with strong adaptabilities to adverse environments; however, the accumulation pattern and biosynthesis basis of soluble sugars and amino acids in this species under osmotic stresses remain elusive. Here, we investigated the physiological responses of a sweet sorghum cultivar to PEG-induced osmotic stresses, analyzed differentially accumulated soluble sugars and amino acids after 20% PEG treatment using metabolome profiling, and identified key genes involved in the biosynthesis pathways of soluble sugars and amino acids using transcriptome sequencing. The results showed that the growth and photosynthesis of sweet sorghum seedlings were significantly inhibited by more than 20% PEG. After PEG treatments, the leaf osmotic adjustment ability was strengthened, while the contents of major inorganic osmolytes, including K⁺ and NO₃⁻, remained stable. After 20% PEG treatment, a total of 119 and 188 differentially accumulated metabolites were identified in the stems and leaves, respectively, and the accumulations of soluble sugars such as raffinose, trehalose, glucose, sucrose, and melibiose, as well as amino acids such as proline, leucine, valine, serine, and arginine were significantly increased, suggesting that these metabolites should play key roles in osmotic adjustment of sweet sorghum. The transcriptome sequencing identified 1711 and 4978 DEGs in the stems, as well as 2061 and 6596 DEGs in the leaves after 20% PEG treatment for 6 and 48 h, respectively, among which the expressions of genes involved in biosynthesis pathways of sucrose (such as SUS1, SUS2, etc.), trehalose (including TPS6), raffinose (such as RAFS2 and GOLS2, etc.), proline (such as P5CS2 and P5CR), leucine and valine (including BCAT2), and arginine (such as ASS and ASL) were significantly upregulated. These genes should be responsible for the large accumulation of soluble sugars and amino acids under osmotic stresses. This study deepens our understanding of the important roles of individual soluble sugars and amino acids in the adaptation of sweet sorghum to water scarcity. Full article

Cereal and legume mixed cropping has been widely adopted to increase forage production in the sustainable development of agriculture and livestock. Among the different mixed cropping combinations, forage sorghum and lablab bean mixed cropping can be adapted globally. However, knowledge regarding the relation between forage production, interspecific competition, and resource utilization efficiency in the forage sorghum and lablab bean mixed cropping system remains unclear. A 3-year field experiment was conducted in 2020, 2021, and 2022 to investigate the effects of different cropping systems (16.5 kg·ha⁻¹ lablab bean mixed cropping with forage sorghum [SD1], 33.0 kg·ha⁻¹ lablab bean mixed cropping with forage sorghum [SD2], 49.5 kg·ha⁻¹ lablab bean mixed cropping with forage sorghum [SD3], 66.0 kg·ha⁻¹ lablab bean mixed cropping with forage sorghum [SD4], sole forage sorghum [SS], and sole lablab bean [DD]) on forage production, forage quality, competition parameters, water use efficiency (WUE), and radiation use efficiency (RUE). The results obtained revealed that mixed cropping practices enhanced forage yield by mitigating soil water depletion and optimizing canopy structures. Specifically, SD3 treatment was an efficient farming practice that increased system dry matter yield by 32.6–67.5%, crude protein yield by 12.5–15.1%, WUE by 9.2–67.4%, and RUE by 39.6–38.2% compared with other treatments. In addition, SD4 treatment increased crude protein content by 11.1% compared with forage sorghum monocropping; however, there were no significant differences in crude protein between SD3 and SD4 mixed cropping systems. The land equivalent ratio values were greater than one when forage sorghum was mixed with lablab bean, especially for the SD3 system (averaged 1.43). In addition, forage sorghum was more dominant and had higher aggressiveness (0.65) and competitive ratios (3.44) than lablab bean. This indicates that mixing cereals with legumes enhances RUE by interspecific competition. Consequently, the SD3-mixed cropping system is recommended for supporting the sustainable development of agriculture and livestock production in the arid region of China when considering forage production and nutritional quality. Full article

Plants reprogramme their proteome to alter cellular metabolism for effective stress adaptation. Intracellular proteomic responses have been extensively studied, and the extracellular matrix stands as a key hub where peptide signals are generated/processed to trigger critical adaptive signal transduction cascades inaugurated at the cell surface. Therefore, it is important to study the plant extracellular proteome to understand its role in plant development and stress response. This study examined changes in the soluble extracellular sub-proteome of sorghum cell cultures exposed to a combination of sorbitol-induced osmotic stress and heat at 40 °C. The combined stress significantly reduced metabolic activity and altered protein secretion. While cells treated with osmotic stress alone had elevated proline content, the osmoprotectant in the combined treatment remained unchanged, confirming that sorghum cells exposed to combined stress utilise adaptive processes distinct from those invoked by the single stresses applied separately. Reactive oxygen species (ROS)-metabolising proteins and proteases dominated differentially expressed proteins identified in cells subjected to combined stress. ROS-generating peroxidases were suppressed, while ROS-degrading proteins were upregulated for protection from oxidative damage. Overall, our study provides protein candidates that could be used to develop crops better suited for an increasingly hot and dry climate. Full article

This study evaluated the refinement of sorghum, corn, and cowpea grains using the processing steps and equipment originally designed for wheat milling that consists of a conventional gradual reduction system. The need to mill these grains resulted from a desire to produce alternative ingredients for developing new fortified blended extruded foods used for food aid programming. Milling of white sorghum grain resulted in a crude protein content of 7.4% (wb) for both whole and coarse-milled flour. The crude protein content in whole fine-milled sorghum was 6.8% (wb), which was significantly lower than that of whole coarse flour at 9.3% (wb). A decrease in the ash content of sorghum flour correlates with the decortication process. However, degermed corn, fine and coarse, had significantly different crude protein content of 6.0 ± 0.2% (wb) and 7.7 ± 0.06% (wb), respectively. Degerming of corn improved the quality of corn flour (fine and coarse) by reducing the crude fat content from 3.3 ± 0.18% (wb) to 1.2 ± 0.02% (wb) and 0.6 ± 0.13% (wb), respectively. This helped increase the starch content from 60.1 ± 0.28% (wb) in raw corn to 74.7 ± 0.93% (wb) and 71.8 ± 0.00% (wb) in degermed fine and coarse corn flour, respectively. Cowpea milling did not produce differences in the milling stream outputs when the crude fat and crude protein were compared. Whole flour from the grains had higher milling yields than decorticated flour. This study demonstrated that a mill dedicated to wheat size reduction can be adapted to refine other grains to high quality. Full article

Drought stress is one of the most severe natural disasters in terms of its frequency, length, impact intensity, and associated losses, making it a significant threat to agricultural productivity. Sorghum (Sorghum bicolor), a C4 plant, shows a wide range of morphological, physiological, and biochemical adaptations in response to drought stress, paving the way for it to endure harsh environments. In arid environments, sorghum exhibits enhanced water uptake and reduced dissipation through its morphological activity, allowing it to withstand drought stress. Sorghum exhibits physiological and biochemical resistance to drought, primarily by adjusting its osmotic potential, scavenging reactive oxygen species, and changing the activities of its antioxidant enzymes. In addition, certain sorghum genes exhibit downregulation capabilities in response to drought stress. Therefore, in the current review, we explore drought tolerance in sorghum, encompassing its morphological characteristics and physiological mechanisms and the identification and selection of its functional genes. The use of modern biotechnological and molecular biological approaches to improving sorghum resistance is critical for selecting and breeding drought-tolerant sorghum varieties. Full article

This study investigated the effects of replacing maize silage (MZS) with high-sugar sorghum silage (HSS) or forage sorghum silage (FSS) without additional grain supplement in the diets of dairy cows on nutrient digestibility, milk composition, nitrogen (N) use, and rumen fermentation. Twenty-four Chinese Holstein cows (545 ± 42.8 kg; 21.41 ± 0.62 kg milk yield; 150 ± 5.6 days in milk) were randomly assigned to three dietary treatments (n = 8 cows/treatment). The cows were fed ad libitum total mixed rations containing (dry matter basis) either 40% MZS (MZS-based diet), 40% HSS (HSS-based diet), or 40% FSS (FSS-based diet). The study lasted for 42 days, with 14 days devoted to adaptation, 21 days to daily feed intake and milk production, and 7 days to the sampling of feed, refusals, feces, urine, and rumen fluid. Milk production was measured twice daily, and digestibility was estimated using the method of acid-insoluble ash. The data were analyzed using a one-way ANOVA in SPSS 22.0 according to a completely randomized design. Dietary treatments were used as fixed effects and cows as random effects. The results indicate that MZS and HSS had greater crude protein but less neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and a lower pH than FSS (p ≤ 0.04). High starch contents in MZS and water-soluble carbohydrate (WSC) contents in HSS were observed (p < 0.01). While the highest starch intake was observed for the MZS-based diet, the highest WSC intake was noted for the HSS-based diet, and the highest NDF, ADF, ADL intake was observed for the FSS-based diet (p ≤ 0.05). The diets, including MZS and HSS, had greater digestibility than that of FSS (p ≤ 0.03). Feeding MZS- and HSS-based diets increased the yield, fat, and protein content of the milk, as well as feed conversion efficiency (p ≤ 0.03). However, feeding the MZS- and HSS-based diets decreased the contents of milk urea N, urinary urea N, and urinary N excretion more than the FSS-based diet (p ≤ 0.05). The N use efficiency tended to increase relative to diets containing MZS and HSS compared with FSS (p = 0.06 and p = 0.09). Ruminal ammonia-N and pH were lower, but total volatile fatty acids, acetate, and propionate were higher in cows fed the HSS- and MZS-based diets compared to those fed the FSS-based diet (p ≤ 0.03). It appears as though replacing MZS with HSS in the diet of cows without additional grain supplements has no negative influence on feed intake, milk yield, N utilization, or ruminal fermentation. Full article

The male sterility line is a vital approach in the genetic breeding of sorghum. The husking process affects the grain’s nutritional composition, emphasizing the intricate relationship between genetic enhancement and dietary requirements. The current study assessed the influence of the Husking Fraction Time Unit (HFTU) process, which was set at 30 (S) and 80 (S) time units per second (S). The study assessed the impact of the (HFTU) process on fifty-one inbred line sorghum race varieties, which implied diverse nutritional profiles considering the pericarp color variations. The assessment of the nutritional profile involved dry matter, total protein, and minerals (P, K, S, Ca, Mg, Na, Fe, Zn, and Mn). The variety groups showed a significance value of p ≤ 0.05, indicating the study hypothesis’s truth. While results demonstrated substantial impacts implied by the Husking Fraction Time Unit (HFTU) technique, the occurrence was noted when the dry matter percentage was increased in the husked products, specifically the endosperm (grits) and bran. Conversely, the protein variation percentage between the bran and endosperm (grits) for the S. bicolor race was calculated at 33.7%. In comparison, the percentage was 11.8% for the Kafirin race. The 80 (S) time unit, on the other hand, had an observable effect on the mineral reconcentration when the Kafirin race had the highest averages of K mg/kg⁻¹, Ca mg/kg⁻¹, and Fe mg/kg⁻¹, which were 5700.5 mg/kg⁻¹, 551.5 mg/kg⁻¹ and 66.5 mg/kg⁻¹, respectively. The results of this study could benefit breeders and nutrition specialists in developing genotypes and processing sorghum grains, promoting research, and aiding several industrial sectors owing to the grain’s adaptability and nutritional properties. Full article