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17 pages, 5956 KB  
Article
Characterization of Sidewalk Trees and Their Mitigation Effect on Extreme Warm Temperatures in a Tropical City of Mexico
by Itzel Castro-Mendoza, José Raúl Vázquez-Pérez, Roberto Antonio Fonseca-Núñez and Carlos Guzmán-López
Forests 2025, 16(9), 1408; https://doi.org/10.3390/f16091408 - 3 Sep 2025
Abstract
In Mexico, an emerging tropical nation, where cities have insufficient vegetation cover and there is little information about their provision of ecosystem services; the study of urban vegetation, as a mitigation strategy, is required. The sidewalk trees in the city of Arriaga (CAR), [...] Read more.
In Mexico, an emerging tropical nation, where cities have insufficient vegetation cover and there is little information about their provision of ecosystem services; the study of urban vegetation, as a mitigation strategy, is required. The sidewalk trees in the city of Arriaga (CAR), considered one of the warmest cities in the Mexican southeast, were counted, measured, and assessed for their effect on surface and air temperatures. There are 6239 sidewalk trees, distributed in 11 families and 13 species; 136 trees were sampled concentrating 77% in three species: Neem, Country almond and Benjamina fig. Therefore, a low H’ (1.73 nats) was obtained. The mitigating effect of tree shade on surface temperature went from 7 °C to 23 °C, depending on the day and hour, while there was not a significant refreshing effect of air temperature because the height of sidewalk trees is controlled with severe pruning to prevent damage to public wiring, causing a similar-sized stratum that traps air under the tree canopy. Consequently, an integral solution that includes, but is not limited to, urban trees is required without leaving aside increasing tree diversity, health, and equitable distribution of trees at CAR. Full article
(This article belongs to the Section Urban Forestry)
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45 pages, 723 KB  
Review
Application of Probiotics in Foods: A Comprehensive Review of Benefits, Challenges, and Future Perspectives
by Mirjana Ž. Grujović, Teresa Semedo-Lemsaddek and Katarina G. Marković
Foods 2025, 14(17), 3088; https://doi.org/10.3390/foods14173088 - 2 Sep 2025
Abstract
The incorporation of probiotics into food products has gained substantial attention, primarily due to their well-documented health benefits such as modulating gut microbiota, enhancing immune responses, and providing potential therapeutic effects. This comprehensive review discusses recent advancements in the application of probiotics in [...] Read more.
The incorporation of probiotics into food products has gained substantial attention, primarily due to their well-documented health benefits such as modulating gut microbiota, enhancing immune responses, and providing potential therapeutic effects. This comprehensive review discusses recent advancements in the application of probiotics in the food industry, focusing on diverse food matrices, technological and regulatory challenges, and consumer acceptance. Particular emphasis is placed on fermentation-based approaches that enhance both sensory and nutritional attributes, while acting as effective delivery systems for viable probiotics. The impact of matrices such as dairy, meat, cereals, plant-based beverages (e.g., soy or almond milk), and solid plant-derived foods (e.g., fermented vegetables) on probiotic survival, sensory properties, and product acceptability is critically examined. Understanding these interactions is crucial for the development of stable, efficacious, and consumer-oriented probiotic-enriched functional foods. Full article
(This article belongs to the Special Issue Application of Probiotics in Foods and Human Health)
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39 pages, 3092 KB  
Article
Borylated Five-Membered Ring Iminosugars: Synthesis, Spectroscopic Analysis, and Biological Evaluation for Glycosidase Inhibition and Anticancer Properties for Application in Boron Neutron Capture Therapy (BNCT)—Part 1
by Kate Prichard, Suzuka Yamamoto, Yuna Shimadate, Kosuke Yoshimura, Barbara Bartholomew, Jayne Gilbert, Jennette Sakoff, Robert Nash, Atsushi Kato and Michela Simone
Pharmaceuticals 2025, 18(9), 1302; https://doi.org/10.3390/ph18091302 - 29 Aug 2025
Viewed by 210
Abstract
Background/Objectives: This article reports pyrrolidine iminosugars of L-gulose absolute stereochemical configuration that are functionalised via N-alkylation to bear boronate ester and boronic acid pharmacophores. Inclusion of boron pharmacophores has been shown to reduce toxicity profiles of drugs and can expand the [...] Read more.
Background/Objectives: This article reports pyrrolidine iminosugars of L-gulose absolute stereochemical configuration that are functionalised via N-alkylation to bear boronate ester and boronic acid pharmacophores. Inclusion of boron pharmacophores has been shown to reduce toxicity profiles of drugs and can expand the range of interactions between drugs and target enzymes. Methods: The synthetic development, detailed spectroscopic analysis, and biological investigation against glycosidase enzymes and cancer cell lines of these novel five-membered ring iminosugars are reported. Results: This family of iminosugars displays selective, moderate-to-weak inhibition (IC50s = 133–501 μM) of β-d-galactosidase (bovine liver) and emerging inhibition of β-d-glucosidases (almond) and (bovine liver). The boronic acid pharmacophore may be suitable for the management of lysosomal storage disorders to support the restoration of biological activity of mutant enzymes via the chaperone-mediated therapy approach. From a structure–activity perspective, the cancer screening revealed slight growth inhibition in a panel of cancer cell lines, with A2780 ovarian carcinoma cells showing the strongest response across all compounds. Beyond the growth inhibition capabilities, the real therapeutic potential of these borylated drugs lies in their switch-on/switch-off activation under BNCT radiotherapeutic conditions. Conclusions: This is an important novel family of drug leads capable of interacting with drug targets via intermolecular and intramolecular interactions, changing shape and electronics. Introduction of organic boron atoms to organic molecules presents significant synthetic and purification challenges, as well as analysis of the equilibria that arise in aqueous systems. We provide a methodology to achieve all this and introduce boron pharmacophores onto carbohydrate scaffolds in a systematic manner to facilitate a more widespread adoption of boron pharmacophores. Full article
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21 pages, 1553 KB  
Article
Influence of Extraction Techniques on Almond Oil Quality: A Comparative Study of Solvent-Extracted and Commercial Products
by Mariola Kozłowska, Diana Mańko-Jurkowska, Bartłomiej Zieniuk and Magdalena Rudzińska
Molecules 2025, 30(17), 3519; https://doi.org/10.3390/molecules30173519 - 28 Aug 2025
Viewed by 356
Abstract
The aim of the study was to compare the quality of almond oils obtained using different extraction methods, including cold solvent extraction, Soxhlet extraction, and the Folch method. Oils were extracted from four commercially available almond-based products—unpeeled almonds, blanched almonds, almond flakes, and [...] Read more.
The aim of the study was to compare the quality of almond oils obtained using different extraction methods, including cold solvent extraction, Soxhlet extraction, and the Folch method. Oils were extracted from four commercially available almond-based products—unpeeled almonds, blanched almonds, almond flakes, and almond protein concentrate—and compared with a commercially refined almond oil. The extracted oils were analyzed for their fatty acid (FA) composition and selected quality parameters, including acid value, peroxide value, p-anisidine value, the TOTOX index, and specific extinction coefficients (K232 and K268). Based on the FA profiles, health-related indices such as atherogenic index, thrombogenic index, and hypocholesterolemic/hypercholesterolemic ratio were also calculated. Additionally, the oxidative stability of the oils was assessed using an accelerated method—pressure differential scanning calorimetry. The obtained results demonstrated that the extraction method had a stronger influence on almond oil quality than the type of raw material. Oil extracted from unpeeled almonds using Soxhlet and cold solvent techniques showed better oxidative stability and more favorable FA profiles, while oils obtained using the Folch method and commercial refined oils exhibited higher levels of primary and secondary oxidation products. These findings were further supported by statistical analyses, which revealed distinct groupings based on oxidation indices and lipid composition. Full article
(This article belongs to the Special Issue Exploring the Natural Antioxidants in Foods)
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33 pages, 7216 KB  
Systematic Review
Blood Lipid Levels in Response to Almond Consumption: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
by Kathy Musa-Veloso, Caroline Gauntlett, Katrina Geronimo, Isabella Vicente and Samuel Pak Lam Ho
Nutrients 2025, 17(17), 2791; https://doi.org/10.3390/nu17172791 - 28 Aug 2025
Viewed by 641
Abstract
Background/Objectives: While the benefits of almond consumption in reducing levels of TC and LDL-C are well established, the effects on additional lipids that have emerged as important predictors of cardiovascular disease, such as ApoB and the ratio of ApoB:ApoA, are not well characterized. [...] Read more.
Background/Objectives: While the benefits of almond consumption in reducing levels of TC and LDL-C are well established, the effects on additional lipids that have emerged as important predictors of cardiovascular disease, such as ApoB and the ratio of ApoB:ApoA, are not well characterized. In this systematic review and meta-analysis, the effects of almond consumption on blood lipids were comprehensively assessed. Methods: On 12 May 2025, ProQuest Dialog™ was used to search ten literature databases (AdisInsight: Trials; Allied & Complementary Medicine™; BIOSIS Previews®; CAB ABSTRACTS; Embase®; Embase Preprints; Foodline®: SCIENCE; FSTA®; MEDLINE®; National Technical Information Service). Randomized controlled trials at least 4 weeks in duration were included if the investigational product was almonds; the control was void of nuts/tree nuts; the subjects were adults without CVD; and blood lipid levels were assessed. Health Canada’s Quality Appraisal Tool for Intervention Studies was used to assess each study’s risk of bias. The mean difference in the effect for each parameter was pooled across studies in a random effects model, using the inverse of the variance as the weighting factor. Results: 36 publications (48 almond–control datasets) representing 2485 participants were included. Almond consumption significantly reduced LDL-C (−0.132 mmol/L; 95% CI: −0.190, −0.075 mmol/L; p < 0.001), TC (−0.160 mmol/L; 95% CI: −0.218, −0.101 mmol/L; p < 0.001), non-HDL-C (−0.204 mmol/L; 95% CI: −0.281, −0.127 mmol/L; p < 0.001), TC:HDL-C (−0.154; 95% CI: −0.246, −0.063; p = 0.001), LDL-C:HDL-C (−0.112; 95% CI: −0.199, −0.026; p = 0.011), ApoB (−4.552 mg/dL; 95% CI: −6.460, −2.645 mg/dL; p < 0.001), and ApoB:ApoA (−0.027; 95% CI: −0.046, −0.008; p = 0.006), with a borderline significant reduction in TG (−0.037 mmol/L; 95% CI: −0.079, 0.005; p = 0.085) and no effects on HDL-C, ApoA, or Lp[a]. The effects persisted when the analyses were limited to higher quality studies, except for the reduction in TG. Conclusions: Almond consumption improves levels of LDL-C, TC, non-HDL-C, TC:HDL-C, LDL-C:HDL-C, ApoB, and ApoB:ApoA, though dedicated clinical trials are needed to better understand effects on TG levels. Full article
(This article belongs to the Special Issue Nuts for Human: The Role of Nuts in a Healthy Diet)
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31 pages, 2653 KB  
Article
Protective Antioxidant Potential of Argan Oil Versus Other Edible Oils in LPS-Challenged Mouse Heart and Kidney
by Soufiane Rabbaa, Habiba Bouchab, Mounia Tahri-Joutey, Yassir Laaziouez, Youness Limami, Vivien Pires, Boubker Nasser, Pierre Andreoletti, Mustapha Cherkaoui-Malki and Riad El Kebbaj
Int. J. Mol. Sci. 2025, 26(17), 8300; https://doi.org/10.3390/ijms26178300 - 27 Aug 2025
Viewed by 419
Abstract
Oxidative stress plays a key role in tissue damage during inflammation, highlighting the need for effective antioxidant interventions. This study investigates the antioxidant potential of argan oil (AO)—obtained from Argania spinosa (L.) Skeels almonds—in comparison with olive oil (OO), cactus seed oil (CSO), [...] Read more.
Oxidative stress plays a key role in tissue damage during inflammation, highlighting the need for effective antioxidant interventions. This study investigates the antioxidant potential of argan oil (AO)—obtained from Argania spinosa (L.) Skeels almonds—in comparison with olive oil (OO), cactus seed oil (CSO), and colza oil (CO). Quantitative analyses of total polyphenols and pigments—including chlorophylls, carotenoids, and xanthophylls—were conducted alongside antioxidant capacity assessments via DPPH, ABTS, and FRAP assays. The methanolic fraction consistently demonstrated the highest phenolic concentration and antioxidant efficacy across all oils. To establish in vivo relevance, a male C57BL/6J mouse model of acute oxidative stress was induced by lipopolysaccharide (LPS) administration. Pretreatment with oils significantly modulated key oxidative stress biomarkers—SOD, CAT, GPx activities, GSH levels, and lipid peroxidation (MDA)—in both heart and kidney. LPS challenge induced marked oxidative imbalance, notably increasing enzymatic activity and MDA levels, while depleting GSH in the heart and elevating it in the kidney. However, pretreatment with oils effectively restored redox homeostasis, with AO showing particularly potent effects and a stronger regulatory effect observed in the kidney. Hierarchical clustering of z-score-normalized heatmaps revealed distinct oxidative stress signatures, clearly separating LPS-treated heart and kidney tissues from other groups due to heightened oxidative markers. In contrast, oil-treated and oil-combined-with-LPS groups clustered closer to the control, underscoring the protective effect of oils against LPS-induced oxidative stress, with efficiency varying by oil type. Pearson correlation analysis, complemented by multivariate principal component analysis (PCA), further emphasized strong positive associations between antioxidant enzymes (SOD, CAT, GPx) and MDA levels, while GSH exhibited tissue-specific behavior—negatively correlated in the heart but positively in the kidney—highlighting divergent redox regulation between organs. Collectively, AO demonstrated robust cardioprotective and nephroprotective properties, supporting its potential as a natural dietary strategy against inflammation-induced oxidative stress. Full article
(This article belongs to the Special Issue Focus on Antioxidants and Human Diseases)
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25 pages, 690 KB  
Review
Chemical Treatments Tested Against Xylella fastidiosa: Strategies, Successes and Limitations
by Letizia Portaccio, Marzia Vergine, Alessandro Bene, Mariarosaria De Pascali, Erika Sabella, Luigi De Bellis and Andrea Luvisi
Pathogens 2025, 14(9), 840; https://doi.org/10.3390/pathogens14090840 - 23 Aug 2025
Viewed by 583
Abstract
Xylella fastidiosa (Xf) is a Gram-negative bacterium responsible for severe diseases in several commercially significant crops, including olive, grapevine, citrus and almond. Its management is particularly challenging due to its transmission via widespread vector insects, its ability to form biofilms, its [...] Read more.
Xylella fastidiosa (Xf) is a Gram-negative bacterium responsible for severe diseases in several commercially significant crops, including olive, grapevine, citrus and almond. Its management is particularly challenging due to its transmission via widespread vector insects, its ability to form biofilms, its high genetic diversity and, sometimes, latent symptoms. Current control strategies focus on integrated and preventive approaches, including the use of resistant varieties, agronomic practices, and vector control through chemical and biological methods. Direct control of the bacterium has always been a complex challenge that includes strategies to limit vector presence and activity in the field; however, several compounds have recently been evaluated that are able to inhibit biofilm formation and Xf growth. This review provides an up-to-date summary of studies investigating the efficacy of various treatments based on organic compounds, synthetic molecules and salt- or metal-based formulations. By evaluating the results of in vitro and in vivo experiments, the most promising solutions were identified that address the main challenges and limitations of chemical control strategies. These include N-acetylcysteine and zinc- and copper-based formulations, which are effective and potentially transferable to the field for crops such as citrus and olive trees. Antimicrobial peptides and nanoparticles, on the other hand, have demonstrated high efficacy in vitro, although further studies directly in the field are required. The evidence emerging from the analyzed studies offer insights to guide future research towards more effective and sustainable management approaches to mitigate the spread and impact of Xf. Full article
(This article belongs to the Section Bacterial Pathogens)
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16 pages, 4138 KB  
Article
Clonal Micropropagation of Promising Genotypes of Amygdalus communis L. for Population Restoration and Gene Pool Conservation
by Timur Turdiyev, Kumissay Duisenova, Irina Kovalchuk, Aigul Madenova, Saule Baizhumanova, Kamila Yemesheva, Natalya Mikhailenko and Zakir Tuigunov
Horticulturae 2025, 11(9), 999; https://doi.org/10.3390/horticulturae11090999 - 22 Aug 2025
Viewed by 351
Abstract
The southern region of Kazakhstan represents the northernmost boundary of the natural habitat of five wild almond species, among which Amygdalus communis L. is of particular interest due to a range of favorable traits for use in breeding programs and cultivation in the [...] Read more.
The southern region of Kazakhstan represents the northernmost boundary of the natural habitat of five wild almond species, among which Amygdalus communis L. is of particular interest due to a range of favorable traits for use in breeding programs and cultivation in the region. The current distribution range of common almond growth was clarified using GPS to determine precise coordinates, and a schematic map was developed. Monitoring revealed a significant reduction in population size. In the surveyed areas, 54 trees were selected and described. Seed material was collected from 34 genotypes and characterized according to a descriptor. Genotypes A3, A8, and A15 were identified as having favorable trait combinations. To restore populations and preserve the gene pool of Amygdalus communis L., a method of clonal micropropagation was employed. The composition of the nutrient medium was optimized for establishment, multiplication, and rhizogenesis. It was determined that Murashige and Skoog (MS) medium without phytohormones is effective for in vitro establishment (70% regeneration rate). For multiplication, MS medium with 0.5 mg/L BAP (6-benzylaminopurine) was used (with a multiplication rate of 3.5 per explant). For rhizogenesis, MS medium with 0.5 mg/L BAP, 0.02 mg/L gibberellic acid (GA), and 0.1 mg/L IBA (indole-3-butyric acid) was used. A total of 340 clonal Amygdalus communis L. plants with closed root systems were grown for field collection. The research results can be applied for the restoration, propagation, and conservation of populations both in vitro and in situ, as well as for the inclusion of selected high-performing genotypes in breeding programs. Full article
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16 pages, 1808 KB  
Article
White Lupin and Hairy Vetch as Green Manures: Impacts on Yield and Nutrient Cycling in an Organic Almond Orchard
by Soraia Raimundo, Margarida Arrobas, António Castro Ribeiro and Manuel Ângelo Rodrigues
Agronomy 2025, 15(8), 1974; https://doi.org/10.3390/agronomy15081974 - 15 Aug 2025
Viewed by 357
Abstract
Organic farming systems, which prohibit synthetic fertilizers, often rely on legumes for their ability to fix atmospheric nitrogen (N). In orchards, legumes can be established as cover crops between tree rows to enhance nutrient cycling. This study evaluated the effects of two legume [...] Read more.
Organic farming systems, which prohibit synthetic fertilizers, often rely on legumes for their ability to fix atmospheric nitrogen (N). In orchards, legumes can be established as cover crops between tree rows to enhance nutrient cycling. This study evaluated the effects of two legume cover crops, white lupin (Lupinus albus L.) and hairy vetch (Vicia villosa Roth), compared to a Control treatment with conventional tillage, which is the most commonly used method of soil management in the region, in an organically managed almond [Prunus dulcis (Mill.) D.A.Webb] orchard compliant with European Union standards, in an experiment arranged as a completely randomized design. In the first year, kernel yield was highest in the Control treatment (404 kg ha−1), while significantly lower yields were recorded for white lupin (246 kg ha−1) and hairy vetch (283 kg ha−1), likely due to competition for resources between cover crops and trees. In the second year, however, the trend reversed, with cover crop treatments yielding significantly more (Lupin: 313 kg ha−1; Vetch: 296 kg ha−1) than the Control (199 kg ha−1). The cover crops accumulated over 150 kg ha−1 of N in their tissues, enhancing soil N availability and increasing N concentrations in almond leaves. In addition to N, cover crops influenced the cycling of other nutrients, increasing potassium (K) and boron (B) concentrations while reducing calcium (Ca) and manganese (Mn) in plant tissues. Despite being derived from a two-year study, these results highlight the complexity of interpreting cover crop effects, underscoring the need for further long-term research to provide more comprehensive guidance to growers. Full article
(This article belongs to the Section Horticultural and Floricultural Crops)
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23 pages, 5300 KB  
Article
Biodegradable Antioxidant Composites with Almond Skin Powder
by Irene Gil-Guillén, Idalina Gonçalves, Paula Ferreira, Chelo González-Martínez and Amparo Chiralt
Polymers 2025, 17(16), 2201; https://doi.org/10.3390/polym17162201 - 12 Aug 2025
Viewed by 300
Abstract
Almond skin (AS) from industrial almond peeling is considered an agri-food waste with adequate composition to obtain composite films for food packaging due to its richness in polysaccharides, proteins, and phenolic compounds. Composite films based on amorphous polylactic acid (PLA) or partially acetylated [...] Read more.
Almond skin (AS) from industrial almond peeling is considered an agri-food waste with adequate composition to obtain composite films for food packaging due to its richness in polysaccharides, proteins, and phenolic compounds. Composite films based on amorphous polylactic acid (PLA) or partially acetylated polyvinilalcohol (PVA) were obtained by melt blending and compression moulding, incorporating different ratios of defatted AS powder (0, 5, 10, and 15 wt.%). The filler was better integrated in the polar PVA matrix, where more interactions were detected with the filler compounds, affecting glass transition and crystallization of the polymer. The AS particles provided the films with the characteristic colour of the powder and strong UV light-blocking effect, while improving the oxygen barrier capacity of both polymeric matrices (24% in PLA with 15% AS and 42% in PVA with 10% AS). The water vapour permeability increased in PLA (by 192% at 15% AS), but decreased in PVA films, especially with low AS content (by 19% with 5% particles). The filler also provided the PLA and PVA films with antioxidant properties due to its phenolic richness, improving the oxygen barrier capacity of the materials and delaying the unsaturated oil oxidation. This was reflected in the lower peroxide and conjugated dienes and trienes values of the sunflower oil packaged in single-dose bags of the different materials. The high oxygen barrier capacity of the PVA bags mainly controlled the preservation of the oil, which made the effect of the antioxidant AS powder less noticeable. Full article
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18 pages, 798 KB  
Article
The Impact of Nut-Based Plant Beverages on Wheat Bread Quality: A Study of Almond, Hazelnut, and Walnut Beverages
by Anna Wirkijowska, Dorota Teterycz and Piotr Zarzycki
Appl. Sci. 2025, 15(16), 8821; https://doi.org/10.3390/app15168821 - 10 Aug 2025
Viewed by 457
Abstract
Nut-based plant beverages are gaining recognition for their functional properties and nutritional value in bakery applications. This study evaluated the effects of substituting water with hazelnut (BH), walnut (BW), and almond (BA) beverages in wheat bread formulations at four substitution levels (25–100%). Thirteen [...] Read more.
Nut-based plant beverages are gaining recognition for their functional properties and nutritional value in bakery applications. This study evaluated the effects of substituting water with hazelnut (BH), walnut (BW), and almond (BA) beverages in wheat bread formulations at four substitution levels (25–100%). Thirteen bread variants, including a control, were produced using the straight dough method. The impact of substitution on dough performance, crumb structure, texture, color, physicochemical composition, and sensory attributes was evaluated. All nut beverages improved bread yield, with BA100 and BW100 showing the highest values. Crumb moisture was well retained, and baking losses were reduced in some high-substitution variants. Medium-sized pores (0.1–0.9 mm2) dominated crumb structure, particularly in almond-enriched breads, which contributed to desirable loaf volume and crumb elasticity. Walnut beverage significantly darkened the crumb due to natural pigments, while BA and BH maintained lighter tones and enhanced yellowness. Nut-based beverages increased ash and fat content, with BW breads showing the highest caloric values—mainly due to beneficial unsaturated fats. Sensory evaluation confirmed high consumer acceptability, with the highest ratings observed for breads containing 100% walnut and 50–75% almond beverage. These variants demonstrated the most favorable balance of technological performance and nutritional enhancement, underscoring their potential as optimal formulations for clean-label, plant-based bread products. Full article
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26 pages, 9940 KB  
Article
Assessing Model Trade-Offs in Agricultural Remote Sensing: A Review of Machine Learning and Deep Learning Approaches Using Almond Crop Mapping
by Mashoukur Rahaman, Jane Southworth, Yixin Wen and David Keellings
Remote Sens. 2025, 17(15), 2670; https://doi.org/10.3390/rs17152670 - 1 Aug 2025
Viewed by 496
Abstract
This study presents a comprehensive review and comparative analysis of traditional machine learning (ML) and deep learning (DL) models for land cover classification in agricultural remote sensing. We evaluate the reported successes, trade-offs, and performance metrics of ML and DL models across diverse [...] Read more.
This study presents a comprehensive review and comparative analysis of traditional machine learning (ML) and deep learning (DL) models for land cover classification in agricultural remote sensing. We evaluate the reported successes, trade-offs, and performance metrics of ML and DL models across diverse agricultural contexts. Building on this foundation, we apply both model types to the specific case of almond crop field identification in California’s Central Valley using Landsat data. DL models, including U-Net, MANet, and DeepLabv3+, achieve high accuracy rates of 97.3% to 97.5%, yet our findings demonstrate that conventional ML models—such as Decision Tree, K-Nearest Neighbor, and Random Forest—can reach comparable accuracies of 96.6% to 96.8%. Importantly, the ML models were developed using data from a single year, while DL models required extensive training data spanning 2008 to 2022. Our results highlight that traditional ML models offer robust classification performance with substantially lower computational demands, making them especially valuable in resource-constrained settings. This paper underscores the need for a balanced approach in model selection—one that weighs accuracy alongside efficiency. The findings contribute actionable insights for agricultural land cover mapping and inform ongoing model development in the geospatial sciences. Full article
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30 pages, 4423 KB  
Review
Overview of Fatty Acids and Volatiles in Selected Nuts: Their Composition and Analysis
by Gbolahan Alagbe, Klara Urbanova and Olajumoke Alagbe
Processes 2025, 13(8), 2444; https://doi.org/10.3390/pr13082444 - 1 Aug 2025
Viewed by 731
Abstract
Nuts are nutrient-dense foods recognized for their complex chemical composition and associated health benefits. This review provides a comprehensive overview of the botanical classification, morphology, production, and consumption patterns of key nut species, including walnuts, almonds, pistachios, pecans, peanuts, cashews, bitter kola, and [...] Read more.
Nuts are nutrient-dense foods recognized for their complex chemical composition and associated health benefits. This review provides a comprehensive overview of the botanical classification, morphology, production, and consumption patterns of key nut species, including walnuts, almonds, pistachios, pecans, peanuts, cashews, bitter kola, and kola nuts. It emphasizes the fatty acid profiles, noting that palmitic acid (C16:0) is the predominant saturated fatty acid, while oleic acid (C18:1) and linoleic acid (C18:2) are the most abundant monounsaturated and polyunsaturated fatty acids, respectively. The review also details various analytical techniques employed for extracting and characterizing bioactive compounds, which are crucial for assessing nut quality and health benefits. Methods such as Soxhlet extraction, solid-phase microextraction (SPME), supercritical fluid extraction (SFE), gas chromatography (GC-FID and GC-MS), and high-performance liquid chromatography (HPLC) are highlighted. Furthermore, it discusses scientific evidence linking nut consumption to antioxidant and anti-inflammatory properties, improved cardiovascular health, and a reduced risk of type 2 diabetes, establishing nuts as important components in a healthy diet. This review underscores the role of nuts as functional foods and calls for standardized methodologies in future lipidomic and volatilomic studies. Full article
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20 pages, 2181 KB  
Article
Metabarcoding Analysis Reveals Microbial Diversity and Potential Soilborne Pathogens Associated with Almond Dieback and Decline
by André Albuquerque, Mariana Patanita, Joana Amaro Ribeiro, Maria Doroteia Campos, Filipa Santos, Tomás Monteiro, Margarida Basaloco and Maria do Rosário Félix
Plants 2025, 14(15), 2309; https://doi.org/10.3390/plants14152309 - 26 Jul 2025
Viewed by 632
Abstract
Almond decline and dieback have become significant challenges in newly established orchards, with symptoms including internal necrosis, canker, and external gummosis. This work aims to explore the potential fungal and bacterial causative agents through metabarcoding and traditional culture plate isolation across six almond [...] Read more.
Almond decline and dieback have become significant challenges in newly established orchards, with symptoms including internal necrosis, canker, and external gummosis. This work aims to explore the potential fungal and bacterial causative agents through metabarcoding and traditional culture plate isolation across six almond cultivars. Our results emphasize the multifactorial nature of almond decline and dieback, with possible co-infections by opportunistic fungi and bacteria playing a central role. Classical isolation identified 47 fungal species or genera, including Diaporthe amygdali, Diplodia corticola, Phytophthora sp., and several Fusarium species. Almond metabarcoding revealed a more diverse microbial community, highlighting the prevalence of soilborne pathogens such as Neocosmospora rubicola, Dactylonectria estremocensis, and Plectosphaerella niemeijerarum. Soil metabarcoding suggested that these pathogens likely originate from nursery substrates or soils shared with other crops, such as olives and vineyards, that serve as a source of inoculum. ‘Soleta’ generally presented lower richness when compared to the other tested cultivars, suggesting a higher degree of biotic stress and decreased plant resilience. This study highlights the value of integrating NGS approaches to comprehensively study complex diseases and the need for further research on pathogen interactions and cultivar susceptibility for the future development of new sustainable, targeted management strategies in almond orchards. Full article
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33 pages, 1657 KB  
Article
Efficient Assessment and Optimisation of Medium Components Influencing Extracellular Xylanase Production by Pediococcus pentosaceus G4 Using Statistical Approaches
by Noor Lutphy Ali, Hooi Ling Foo, Norhayati Ramli, Murni Halim and Karkaz M. Thalij
Int. J. Mol. Sci. 2025, 26(15), 7219; https://doi.org/10.3390/ijms26157219 - 25 Jul 2025
Viewed by 395
Abstract
Xylanase is an essential industrial enzyme for degrading plant biomass, pulp and paper, textiles, bio-scouring, food, animal feed, biorefinery, chemicals, and pharmaceutical industries. Despite its significant industrial importance, the extensive application of xylanase is hampered by high production costs and concerns regarding the [...] Read more.
Xylanase is an essential industrial enzyme for degrading plant biomass, pulp and paper, textiles, bio-scouring, food, animal feed, biorefinery, chemicals, and pharmaceutical industries. Despite its significant industrial importance, the extensive application of xylanase is hampered by high production costs and concerns regarding the safety of xylanase-producing microorganisms. The utilisation of renewable polymers for enzyme production is becoming a cost-effective alternative. Among the prospective candidates, non-pathogenic lactic acid bacteria (LAB) are promising for safe and eco-friendly applications. Our investigation revealed that Pediococcus pentosaceus G4, isolated from plant sources, is a notable producer of extracellular xylanase. Improving the production of extracellular xylanase is crucial for viable industrial applications. Therefore, the current study investigated the impact of various medium components and optimised the selected medium composition for extracellular xylanase production of P. pentosaceus G4 using Plackett–Burman Design (PBD) and Central Composite Design (CCD) statistical approaches. According to BPD analysis, 8 out of the 19 investigated factors (glucose, almond shell, peanut shell, walnut shell, malt extract, xylan, urea, and magnesium sulphate) demonstrated significant positive effects on extracellular xylanase production of P. pentosaceus G4. Among them, glucose, almond shells, peanut shells, urea, and magnesium sulphate were identified as the main medium components that significantly (p < 0.05) influenced the production of extracellular xylanase of P. pentosaceus G4. The optimal concentrations of glucose, almond shells, peanut shells, urea, and magnesium sulphate, as determined via CCD, were 26.87 g/L, 16 g/L, 30 g/L, 2.85 g/L, and 0.10 g/L, respectively. The optimised concentrations resulted in extracellular xylanase activity of 2.765 U/mg, which was similar to the predicted extracellular xylanase activity of 2.737 U/mg. The CCD-optimised medium yielded a 3.13-fold enhancement in specific extracellular xylanase activity and a 7.99-fold decrease in production costs compared to the commercial de Man, Rogosa and Sharpe medium, implying that the CCD-optimised medium is a cost-effective medium for extracellular xylanase production of P. pentosaceus G4. Moreover, this study demonstrated a positive correlation between extracellular xylanase production, growth, lactic acid production and the amount of sugar utilised, implying the multifaceted interactions of the physiological variables affecting extracellular xylanase production in P. pentosaceus G4. In conclusion, statistical methods are effective in rapidly assessing and optimising the medium composition to enhance extracellular xylanase production of P. pentosaceus G4. Furthermore, the findings of this study highlighted the potential of using LAB as a cost-effective producer of extracellular xylanase enzymes using optimised renewable polymers, offering insights into the future use of LAB in producing hemicellulolytic enzymes. Full article
(This article belongs to the Section Biochemistry)
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