Search Results (337)

A straightforward ex vivo approach has been developed and refined to enable high-resolution imaging and quantitative assessment of motile cilia function in mouse airway epithelial tissue, allowing critical insights into cilia motility and cilia generated flow using different mouse models or following different sample treatments. In this method, freshly excised mouse trachea is cut longitudinally through the trachealis muscle which is then sandwiched between glass coverslips within a thin silicon gasket. By orienting the tissue along its longitudinal axis, the natural curling of the trachealis muscle helps maintain the sample in a configuration optimal for imaging along the full tracheal length. High-speed video microscopy, utilizing differential interference contrast (DIC) optics and a fast digital camera capturing at >200 frames per second is then used to record ciliary motion. This enables detailed measurement of both cilia beat frequency (CBF) and waveform characteristics. The application of 1 µm microspheres to the bathing media during imaging allows for additional analysis of fluid flow generated by ciliary activity. The entire procedure typically takes around 40 min to complete per animal: ~30 min for tissue harvest and sample mounting, then ~10 min for imaging samples and acquiring data. Full article

Accurate short-term electricity load forecasting is essential for efficient energy management, grid reliability, and cost optimization. This study presents a comprehensive comparison of five supervised learning models—Convolutional Neural Network (CNN), Long Short-Term Memory (LSTM), Gated Recurrent Unit (GRU), a hybrid (CNN-LSTM) architecture, and Light Gradient Boosting Machine (LightGBM)—using multivariate data from the Greek electricity market between 2015 and 2024. The dataset incorporates hourly load, temperature, humidity, and holiday indicators. Extensive preprocessing was applied, including K-Nearest Neighbor (KNN) imputation, time-based feature extraction, and normalization. Models were trained using a 70:20:10 train–validation–test split and evaluated with standard performance metrics: MAE, MSE, RMSE, NRMSE, MAPE, and $R^2$. The experimental findings show that LightGBM beat deep learning (DL) models on all evaluation metrics and had the best MAE (69.12 MW), RMSE (101.67 MW), and MAPE (1.20%) and the highest $R^2$ (0.9942) for the test set. It also outperformed models in the literature and operational forecasts conducted in the real world by ENTSO-E. Though LSTM performed well, particularly in long-term dependency capturing, it performed a bit worse in high-variance periods. CNN, GRU, and hybrid models demonstrated moderate results, but they tended to underfit or overfit in some circumstances. These findings highlight the efficacy of LightGBM in structured time-series forecasting tasks, offering a scalable and interpretable alternative to DL models. This study supports its potential for real-world deployment in smart/distribution grid applications and provides valuable insights into the trade-offs between accuracy, complexity, and generalization in load forecasting models. Full article

Microwave frequency comb (MFC) optimization for frequency-modulated continuous-wave (FMCW) generation by period-one (P1) dynamics with dual-loop optical feedback are numerically investigated. The linewidth, the side peak suppression (SPS) ratio, and the comb contrast are adopted to quantitatively evaluate the optimization performance, which directly influence the phase stability, spectral purity and repeatability of the MFC. The results show that intensity modulation of the optical injection can generate a sweepable FMCW signal after photodetection via the optical beat effect. When optical feedback loops are introduced, the single-loop configuration can reduce the phase noise of the FMCW signal whereas a dual-loop configuration exploits the Vernier effect to achieve further linewidth reduction and wide tolerance to the feedback strength. Finally, for both the SPS ratio and comb contrast, the dual-loop configuration achieves a higher SPS ratio and maintains high contrast across a wide range of optical feedback loop delays, which outperforms the loop time tolerance of the single-loop configuration. Full article

Heart Rate Variability (HRV) analysis allows for assessing autonomic control from the beat-by-beat dynamics of the time series of cardiac intervals. However, some HRV indices may strongly correlate with the mean heart rate, possibly flawed by the interpretation of HRV changes in terms of autonomic control. Therefore, this study aims to (1) investigate how HRV indices of fluctuation amplitude and multiscale complex dynamics of cardiac time series faithfully describe the autonomic control at different heart rates through a mathematical model of the generation of cardiac action potentials driven by realistically synthesized autonomic modulations; and (2) propose an alternative procedure of HRV analysis less sensitive to the mean heart rate. Results on the synthesized series confirm a strong dependency of amplitude indices of HRV on the mean heart rate due to a nonlinearity in the model, which can be removed by our procedure. Application of our procedure to real cardiac intervals recorded in different postures suggests that the dependency of these indices on the heart rate may importantly affect the physiological interpretation of HRV. By contrast, multiscale complexity indices do not substantially depend on the heart rate provided that multiscale analyses are defined on a time- rather than a beat-basis. Full article

Background: The global surge in obesity has reignited the need for larger cuff sizes to avoid inaccurate blood pressure (BP) readings and inappropriate antihypertensive treatment. More precise methods are essential. Forearm BP measurement has emerged as a promising alternative, showing strong correlation with noninvasive beat-to-beat monitoring. This study aimed to validate the digital oscillometric method on the forearm, comparing results to brachial artery readings in hypertensive patients with prediabetes and obesity. Methods: A non-randomized, open, cross-sectional observational study was conducted with 72 hypertensive individuals presenting with obesity and prediabetes. BP was measured using oscillometric devices on both the arm and forearm, following standardized protocols. Data were analyzed using Pearson correlation and Bland–Altman agreement tests. Results: Arm and forearm BP readings showed high agreement: r = 0.86 for systolic BP (SBP), r = 0.93 for diastolic BP (DBP), and r = 0.90 for mean arterial pressure (MAP). Bland–Altman analysis confirmed equivalence (SBP: p = 0.8, DBP: p = 0.3, MAP: p = 0.2). Conclusions: Forearm BP measurement is a reliable and effective alternative, particularly for hypertensive patients with prediabetes and obesity. It offers accurate readings, reduces treatment risks, and supports better clinical decisions. Broader studies are needed for generalization. Full article

A non-intrusive mattress based on an optical-fiber Michelson interferometer is designed for daily sleep monitoring. The optical phase signal of the optical-fiber Michelson interferometer caused by the heartbeat and respiration is demodulated by the phase-generated carrier (PGC) method. The physiological signals and vital indicators including heart rate (HR), respiration rate (RR), and signal energy (SE) are extracted from the optical phase by algorithmic processing. A series of experiments are conducted to confirm the feasibility of the mattress for sleep monitoring. The mattress not only can achieve HR and RR counting, but also can record the waveform of the sleep-induced signal accurately. The body states can also be distinguished by the SE. In an all-night sleep monitoring experiment, the HR measured by the mattress is compared with the HR measured by a commercial smart band, showing a maximum error of 6 bpm (beat per minute). The designed mattress based on an optical-fiber Michelson interferometer shows good performance and great potential in non-intrusive sleep monitoring. Full article

Interferometric signals in space-borne Gravitational Wave Detectors are measured by digital phasemeters. The phasemeter processes signals generated by multiple interferometers, with its primary function being micro-radian level phase measurements. The Science Interferometer is responsible for inter-spacecraft measurements, including relative ranging, absolute ranging, laser communication, and clock noise transfer. Since the scientific interferometer incorporates multiple functions and various signals are simultaneously coupled into the heterodyne signal, establishing a suitable evaluation environment is a crucial foundation for achieving micro-radian level phase measurement during ground testing and verification. This paper evaluates the phase measurement noise of the science interferometer by simulating the heterodyne signal and establishing a test environment. The experimental results show that when the simulated heterodyne signal contains the main beat-note, upper and lower sideband beat-notes, and PRN modulation simultaneously, the phase measurement noise of the main beat-note, upper and lower sideband beat-notes all reach 2π μrad/Hz^1/2@(0.1 mHz–1 Hz), meeting the requirements of the space gravitational wave detection mission. An experimental verification platform and performance reference benchmark have been established for subsequent research on the impact of specific noise on phase measurement performance and noise suppression methods. Full article

Vertical slot fishways are a crucial measure to mitigate the blockage of fish migration caused by hydraulic engineering infrastructures, but their passage efficiency is often hindered by the complex interactions between fish behavior and hydrodynamic conditions. This study combines computational fluid dynamics (CFD) simulations with behavioral laboratory experiments to identify the hydrodynamic characteristics and swimming strategies of three types of fishways—Central Orifice Vertical Slot (COVS), Standard Vertical Slot (SVS), and L-shaped Vertical Slot (LVS)—using the endangered species Schizothorax prenanti from the upper Yangtze River as the study subject. The results revealed that (1) a symmetric and stable flow field was formed in the COVS structure, yet the passage ratio was the lowest (50%); in the SVS structure, high turbulent kinetic energy (peak of 0.03 m²/s²) was generated, leading to a significant increase in the fish’s tail-beat angle and amplitude (p < 0.01), with the passage time extending to 10.2 s. (2) The LVS structure induced a controlled vortex formation and created a reflux zone with low turbulent kinetic energy, facilitating a “wait-and-surge” strategy, which resulted in the highest passage ratio (70%) and the shortest passage time (6.1 s). (3) Correlation analysis revealed that flow velocity was significantly positively correlated with absolute swimming speed (r = 0.80), turbulent kinetic energy, and tail-beat parameters (r > 0.68). The LVS structure achieved the highest passage ratio and shortest transit time for Schizothorax prenanti, demonstrating its superior functionality for upstream migration. This design balances hydrodynamic complexity with low-turbulence refuge zones, providing a practical solution for eco-friendly fishways. Full article

Predicting short-term buy and sell signals in financial markets remains a significant challenge for algorithmic trading. This difficulty stems from the data’s inherent volatility and noise, which often leads to spurious signals and poor trading performance. This paper presents a novel algorithmic trading model for silver that combines fine-tuned Convolutional Neural Networks (CNNs) with a decision filter based on the Relative Strength Index (RSI). The technique allows for the prediction of buy and sell points by turning time series data into chart images. Daily silver price per ounce data were turned into chart images using technical analysis indicators. Four pre-trained CNNs, namely AlexNet, VGG16, GoogLeNet, and ResNet-50, were fine-tuned using the generated image dataset to find the best architecture based on classification and financial performance. The models were evaluated using walk-forward validation with an expanding window. This validation method made the tests more realistic and the performance evaluation more robust under different market conditions. Fine-tuned VGG16 with the RSI filter had the best cost-adjusted profitability, with a cumulative return of 115.03% over five years. This was nearly double the 61.62% return of a buy-and-hold strategy. This outperformance is especially impressive because the evaluation period was mostly upward, which makes it harder to beat passive benchmarks. Adding the RSI filter also helped models make more disciplined decisions. This reduced transactions with low confidence. In general, the results show that pre-trained CNNs fine-tuned on visual representations, when supplemented with domain-specific heuristics, can provide strong and cost-effective solutions for algorithmic trading, even when realistic cost assumptions are used. Full article

Optical interferometry provides high-precision displacement and angle measurement solutions for a wide range of cutting-edge industrial applications. One of the key factors to achieve such precision lies in highly accurate optical encoder signal processing, as well as the calibration and compensation techniques customized for specific measurement principles. Optical interferometric techniques, including laser interferometry and grating interferometry, are usually classified into homodyne and heterodyne systems according to their working principles. In homodyne interferometry, the displacement is determined by analyzing the phase variation of amplitude-modulated signals, and common demodulation methods include error calibration methods and ellipse parameter estimation methods. Heterodyne interferometry obtains displacement information through the phase variation of beat-frequency signals generated by the interference of two light beams with shifted frequencies, and its demodulation techniques include pulse-counting methods, quadrature phase-locked methods, and Kalman filtering. This paper comprehensively reviews the widely used signal processing techniques in optical interferometric measurements over the past two decades and conducts a comparative analysis based on the characteristics of different methods to highlight their respective advantages and limitations. Finally, the hardware platforms commonly used for optical interference signal processing are introduced. Full article

Terahertz (THz) frequencies, spanning from 0.1 to 1 THz, are poised to play a pivotal role in the development of future 6G wireless communication systems. These systems aim to utilize photonic technologies to enable ultra-high data rates—on the order of terabits per second—while maintaining low latency and high efficiency. In this work, we present a novel photonic method for generating sub-THz vector signals within the THz band, employing a semiconductor optical amplifier (SOA) and phase modulator (PM) to create an optical frequency comb, combined with in-phase and quadrature (IQ) modulation techniques. We demonstrate, both through simulation and experimental setup, the generation and successful transmission of a 0.1 THz vector. The process involves driving the PM with a 12.5 GHz radio frequency signal to produce the optical comb; then, heterodyne beating in a uni-traveling carrier photodiode (UTC-PD) generates the 0.1 THz radio frequency signal. This signal is transmitted over distances of up to 30 km using single-mode fiber. The resulting 0.1 THz electrical vector signal, modulated with quadrature phase shift keying (QPSK), achieves a bit error ratio (BER) below the hard-decision forward error correction (HD-FEC) threshold of ${3.8 \times 10}^{-3}$. To the best of our knowledge, this is the first experimental demonstration of a 0.1 THz photonic vector THz wave based on an SOA and a simple PM-driven optical frequency comb. Full article

Previous work used machine learning (ML) to estimate past and current alfalfa yields and showed that domain adaptation (DA) with data synthesis shows promise in classifying yields as high, medium, or low. The current work uses similar techniques to forecast future alfalfa yields. A novel technique is proposed for forecasting alfalfa time series data that exploits stationarity and predicts differences in yields rather than the yields themselves. This forecasting technique generally provides more accurate forecasts than the established ARIMA family of forecasters for both univariate and multivariate time series. Furthermore, this ML-based technique is potentially easier to use than the ARIMA family of models. Also, previous work is extended by showing that DA with data synthesis also works well for predicting continuous values, not just for classification. The novel scale-invariant tabular synthesizer (SITS) is proposed, and it is competitive with or superior to other established synthesizers in producing data that trains strong models. This synthesis algorithm leads to R scores over 100% higher than an established synthesizer in this domain, while ML-based forecasters beat the ARIMA family with symmetric mean absolute percent error (sMAPE) scores as low as 12.81%. Finally, ML-based forecasting is combined with DA (ForDA) to create a novel pipeline that improves forecast accuracy with sMAPE scores as low as 9.81%. As alfalfa is crucial to the global food supply, and as climate change creates challenges with managing alfalfa, this work hopes to help address those challenges and contribute to the field of ML. Full article

Background: Chronic diseases significantly burden healthcare systems due to the need for long-term treatment. Early diagnosis is critical for effective management and minimizing risk. The current traditional diagnostic approaches face various challenges regarding efficiency and cost. Digitized healthcare demonstrates several opportunities for reducing human errors, increasing clinical outcomes, tracing data, etc. Artificial Intelligence (AI) has emerged as a transformative tool in healthcare. Subsequently, the evolution of Generative AI represents a new wave. Large Language Models (LLMs), such as ChatGPT, are promising tools for enhancing diagnostic processes, but their potential in this domain remains underexplored. Methods: This study represents the first systematic evaluation of ChatGPT’s performance in chronic disease prediction, specifically targeting heart disease and diabetes. This study compares the effectiveness of zero-shot, few-shot, and CoT reasoning with feature selection techniques and prompt formulations in disease prediction tasks. The two latest versions of GPT4 (GPT-4o and GPT-4o-mini) are tested. Then, the results are evaluated against the best models from the literature. Results: The results indicate that GPT-4o significantly beat GPT-4o-mini in all scenarios regarding accuracy, precision, and F1-score. Moreover, a 5-shot learning strategy demonstrates superior performance to zero-shot, few-shot (3-shot and 10-shot), and various CoT reasoning strategies. The 5-shot learning strategy with GPT-4o achieved an accuracy of 77.07% in diabetes prediction using the Pima Indian Diabetes Dataset, 75.85% using the Frankfurt Hospital Diabetes Dataset, and 83.65% in heart disease prediction. Subsequently, refining prompt formulations resulted in notable improvements, particularly for the heart dataset (5% performance increase using GPT-4o), emphasizing the importance of prompt engineering. Conclusions: Even though ChatGPT does not outperform traditional machine learning and deep learning models, the findings highlight its potential as a complementary tool in disease prediction. Additionally, this work provides value by setting a clear performance baseline for future work on these tasks Full article

Hypertension and blood pressure variability (BPV) are major risk factors for cardiovascular disease (CVD). Single-channel photoplethysmography (PPG) has emerged as a promising daily blood pressure (BP) monitoring tool. However, estimating BP trends presents challenges due to complex temporal dependencies and continuous fluctuations. Traditional methods often address BP prediction as isolated tasks and focus solely on temporal dependencies within a limited time window, which may fall short of capturing the intricate BP fluctuation patterns implied in varying time spans, particularly amidst constant BP variations. To address this, we propose a novel deep learning model featuring a two-stage architecture and a new input structure called contextual cycles. This model estimates beat-to-beat systolic blood pressure (SBP) trends as a sequence prediction task, transforming the output from a single SBP value into a sequence. In the first stage, parallel ResU Blocks are utilized to extract fine-grained features from each cycle. The generated feature vectors are then processed by Transformer layers with relative position encoding (RPE) to capture inter-cycle interactions and temporal dependencies in the second stage. Our proposed model demonstrates robust performance in beat-to-beat SBP trend estimation, achieving a mean absolute error (MAE) of 3.186 mmHg, a Pearson correlation coefficient applied to sequences (${\mathrm{R}}_{\mathrm{seq}}$) of 0.743, and a variability error (VE) of 1.199 mmHg. It excels in steady and abrupt substantial fluctuation states, outperforming baseline models. The results reveal that our method meets the requirements of the AAMI standard and achieves grade A according to the BHS standard. Overall, our proposed method shows significant potential for reliable daily health monitoring. Full article

Multiciliated cells generate fluid flow along epithelial surfaces, and defects in their development or function cause primary ciliary dyskinesia. The fluid flow is generated by the coordinated beating of motile cilia, which are microtubule-based organelles. The base of each cilium, the basal body, is anchored to the apical cell membrane and surrounded by a dense apical cytoskeleton of actin, microtubules, and intermediate filaments. Several cell adhesion proteins play a role in the connection of the basal body to the apical cytoskeleton. Here, we show that the desmosomal protein plakophilin3, a member of the armadillo family of proteins, localizes to the striated rootlet in Xenopus laevis multiciliated cells. Knockdown of plakophilin 3 leads to significant defects in cilia-generated fluid flow and basal body docking. These defects are cell-autonomous and independent of cell intercalation and gross changes in the actin cytoskeleton. These findings suggest a crucial role for PKP3 in basal body apical migration and docking in multiciliated cells, highlighting a novel connection between desmosomal proteins and ciliary function. Full article