Search Results (54)

Cyanobacteria-based bioinoculants represent a sustainable solution for enhancing soil fertility and crop productivity. This research assessed the biofertilizing potential of two indigenous nitrogen-fixing cyanobacteria strains (Nostoc punctiforme Har. and Anabaena cylindrica Lemmerm.) on tomato growth and yield. A greenhouse experiment was conducted to study their effects on soil properties, plant growth and physiology, and fruit yield/quality. The strains were applied individually, as a consortium, or combined with organic or mineral fertilizers at half the standard dose (50%). All bioinoculants improved soil fertility, plant growth, and fruit yield/quality compared to the control. The most significant improvement was observed in the consortium amended with 50% of conventional fertilizer (compost or NPK), compared with individual strains. Correlation analysis revealed strong positive associations between photosynthetic pigments, plant productivity, and fruit biochemical traits, indicating coordinated physiological responses under the applied treatments. The results demonstrated that the consortium of diazotrophic terrestrial cyanobacteria possesses tomato biofertilizer properties that can be efficiently used in crop production. These findings suggest that such formulations offer a cost-effective approach to tomato cultivation and present a sustainable alternative for integrated and optimized fertilizer management. Full article

Soil microorganisms play critical roles in mediating soil fertility. Exploring the effect of fertilization on soil microbial communities is of great importance to comprehend the sustainability of agriculture. However, the impacts of the application of different fertilization techniques on soil microbial communities remain ambiguous due to inconsistent findings across studies. In this study, we investigated changes in soil microbial communities under different fertilization techniques (chemical fertilizer (CK), organic fertilizer (OF), Bacillus-amended biofertilizer (BF), and Trichoderma-amended biofertilizer (MF)) and analyzed the link between soil fertility improvement and crop yield increase from a microbial perspective. Compared to the CK treatment, the BF and MF treatments increased corn yields by 16.07% and 12.98%, and soybean yields by 17.48% and 15.32%, respectively. BF tends to increase soil available phosphorus, whereas MF demonstrates a more pronounced enhancement in both available phosphorus and NH₄⁺-N contents. These differential effects were primarily linked to changes in the microbial community. Specifically, BF significantly enriched Bacillus, Rhodanobacter, Massilia, Mortierella, and Tetracladium, while the MF selectively increased the abundances of Burkholderia-Caballeronia-Paraburkholderia, Trichoderma, Penicillium, and Sistotrema. Co-occurrence network analysis revealed that biofertilizers enhanced microbial network stability and complexity compared to conventional fertilization techniques. Moreover, structural equation modeling (SEM) confirmed strong and positive relationships between crop yields and the abundances of specific probiotic microorganisms. These findings elucidate the mechanism-specific roles of biofertilizers in agricultural systems and provide novel insights for developing targeted biofertilizer formulations to advance sustainable agricultural practices. Full article

The nutrient-rich composition of seaweeds and lichens makes them well-suited for agricultural applications. Their use as alternatives to synthetic fertilizers contributes to sustainable agricultural production, enabling farmers to adopt ecological practices while maintaining or increasing crop productivity. This review aims to highlight the status and trends of research, along with a literature analysis on the application of these biomasses in sustainable agriculture. A bibliometric analysis was performed based on two databases (Scopus and Web of Science) to overview the main research topics regarding the use of biomasses studied in agriculture, thus providing useful information for future research. The biochemical composition and agricultural applications of these biomasses have been highlighted. The analysis shows that these biomasses are rich of nutrient compounds, revealing their roles and mechanisms of action on the chemical, nutritional properties, and soil microbial activities and their effect on plant growth, using various extraction and application methods. It also highlighted the potential of seaweeds for protection against biotic and abiotic stresses. In light of all the data presented in this review, it is possible to stimulate farmers’ interest in using seaweeds and lichens as natural fertilizers, with a focus on sustainable and ecological agriculture mainly in developing countries. Full article

Organic farming relies on sustainable, eco-friendly practices that promote soil health, biodiversity, and climate resilience. Bioresources—derived from plants, animals, and microorganisms—are pivotal in replacing synthetic inputs with natural alternatives. This review presents an integrated analysis of bioresources, highlighting their classification, functionality, and role in organic systems through biofertilizers, biopesticides, organic amendments, and bioenergy. Despite their potential, challenges such as knowledge gaps, limited scalability, and technical constraints hinder their widespread adoption. The review emphasizes the ecological, economic, and social benefits of bioresource integration while identifying critical barriers and proposing strategic directions for research, policy, and practice. By addressing these gaps, bioresources can enhance nutrient cycling, pest management, and soil regeneration, offering a viable path toward sustainable agriculture. This synthesis supports the development of context-specific, circular, and resilient organic farming systems that align with global sustainability goals. Full article

Despite growing interest in improving soil health on cocoa farms, applied research on the impacts of specific amendments on soil and plant outcomes is lacking. An integrated assessment of the impacts of two different organic amendments (compost and vermicompost) and a microbial biofertilizer on soil physical, chemical, and biological properties, as well as cocoa flowering, fruit set, and yield, was conducted in Guayaquil, Ecuador. Complementary culture-dependent and culture-independent methods were used to assess the impacts of amendments on microbial diversity, community composition, and specific taxa. Compost or vermicompost application affected soil chemical properties, including potassium, phosphorus, and sodium, and had small but significant effects on fungal beta diversity. Biofertilizer application slightly lowered soil pH and altered the total abundance of specific taxonomic groups including Azotobacter sp. and Trichoderma sp., with borderline significant effects on Azospirillum sp., Lactobacillus sp., Pseudomonas sp., calcium-solubilizing bacteria, and phosphorus-solubilizing bacteria. Amplicon sequencing (16S, ITS) identified 15 prokaryotic and 68 fungal taxa whose relative abundance was influenced by organic amendments or biofertilizer. Biofertilizer application increased cherelle formation by 19% and monthly harvestable pod counts by 11% despite no impact on flowering index or annual pod totals. This study highlights the tangible potential of microbiome optimization to simultaneously improve on-farm yield and achieve soil health goals on cocoa farms. Full article

Biofertilizers play a crucial role in promoting sustainable agriculture in China; however, comprehensive quantification of their effects and limitations in field conditions remain unclear. In this study, a meta-analysis encompassing 1818 comparisons from 107 studies was conducted to quantify their systematic effects in field conditions in China. The results demonstrated that biofertilizers enhanced crop yields across 21 of the 23 investigated crops, with notable increases in millet (+65.42%), vegetables (e.g., Chinese cabbage +35.57%, ginger +39.18%), and legumes (kidney beans +54.03%), while cotton and rapeseed showed non-significant improvements. Nutritional quality was also improved, as evidenced by elevated levels of vitamin C (14.61%), protein (16.61%), and carotenoids (15.18%), alongside a reduction in nitrate content (21.94%). Soil health was significantly improved through increased organic matter (16.64%), enhanced enzymatic activities (urease: 57.60%; phosphatase: 43.51%), and a proliferation of beneficial microbes (bacteria: 157.10%; fungi: 30.28%), while pathogenic organisms were suppressed by 51.81%. The observed yield improvements were attributed to enhanced nutrient availability (total nitrogen: 16.67%; available phosphorus: 10.98%), optimized root growth (19.23% increase in volume), and a reduction in disease incidence (42.52%). The efficacy of biofertilizers was maximized when they were used in conjunction with organic amendments, resulting in a 29.20% increase in yield, particularly when applied prior to planting. These results show that biofertilizers boost productivity, quality, and soil functionality, depending on their production and field management practices. Their effectiveness is tied to optimizing soil properties and suppressing pathogens, providing strategies for sustainable agriculture in China. Full article

Seaweeds and their derived products have long been valued in organic agriculture, serving roles in biofertilizers, biostimulants, and soil conditioners due to their rich content of bioactive compounds. With increasing concerns over the negative impacts of synthetic agrochemicals on food security and environmental health, seaweeds offer a sustainable alternative for improving soil fertility and crop productivity. This review synthesizes recent findings on the use of seaweeds to enhance soil physicochemical properties, stimulate beneficial microbial activity, and improve nutrient availability. Furthermore, it highlights how seaweed applications can mitigate various abiotic stresses, such as droughts, salinity, and nutrient deficiency, by enhancing antioxidant defenses and promoting physiological and biochemical resilience in plants. Key agronomic benefits include improved seed germination, root development, photosynthesis, biomass accumulation, and yield performance. By acting as natural soil amendments, seaweeds support sustainable soil management and contribute to long-term agricultural resilience. This review emphasizes the urgent need for standardized application strategies and integrated research to unlock the full potential of seaweed-based solutions in sustainable farming systems. Full article

The conversion of aquatic biomass into biochar offers a sustainable strategy for improving soil fertility and mitigating ecological imbalances caused by its rapid proliferation. In this study, Typha angustifolia, a widely distributed aquatic weed, was utilized for biochar production. Three biochar types (TABC400, TABC500, and TABC600) were synthesized through pyrolysis at 400 °C, 500 °C, and 600 °C temperature. It was hypothesized that Typha angustifolia biochar would positively influence the growth and development of okra (Abelmoschus esculentus L.). The results demonstrate that biochar yield subsequently decreases with increasing pyrolysis temperature, with the highest yield at 400 °C temperature (49.03%), followed by 500 °C (38.02%) and 600 °C temperature (32.01%). However, carbon content 67.01 to 83.12%, higher heating value (17.31 to 27.42 MJ/kg), and mineral contents (K, Mg, P, Ca, Fe, Cu, Zn) increase significantly with higher pyrolysis temperature. However, oxygen, hydrogen, nitrogen, bulk density, moisture contents, and volatile context exhibited an inverse relationship with pyrolysis temperature, highlighting biochar stability and its potential for soil amendment. Among the three synthesized biochar, the 4%TABC600 (600 °C) revealed the most substantial improvement in plant height (110.11 ± 4.12 cm), plant dry biomass (6.12 ± 0.41 gm), and chlorophyll contact (39.34 ± 3.33 SPAD values), whereas the 2% and 6% TABC600 demonstrated significant influence on fruit yield (9.11 ± 2.11 gm) and fruit weight (750.44 ± 7.83 g), and chlorophyll contact (32–38 SPAD values). Based on our results, we can conclude that Typha angustifolia biochar prepared at 600 °C (TABC600) has great potential as a biofertilizer, promoting soil fertility and growth and development of crops, particularly for vegetable cultivation such as okra. Full article

Spent coffee ground (SCG) is a main byproduct of the coffee industry and has been revalorized as a source of value-added products. The direct application of SCG as a soil amendment has drawn much attention in recent years as a more environmentally sustainable option. In this work, a comprehensive review of studies on the agricultural application of SCG is presented. The aims of this review were (1) to summarize the impacts of raw SCG on soil health and to identify the factors limiting its direct soil use, (2) to outline methods that are often used to upgrade SCG for soil application, and (3) to highlight the potential of SCG and its derivatives as a biocontrol agent and biofertilizer. The results indicate that raw SCG could improve certain soil health attributes, but plant growth is often limited owing to its phytotoxicity and induced N immobilization. To eliminate phytotoxicity, composting, vermicomposting, and thermochemical treatments have been employed but to a limited extent. SCG and its derivatives may be used as biocontrol agents for weeds, soil-borne pathogens, and pests. Novel bio-organic fertilizers based on SCG have also been developed. Future research should focus on the long-term effects of SCG and its derivatives as soil amendments under real scenarios and the underlying mechanisms, particularly the soil–plant–microorganism interactions. Full article

Azolla is a substitute compost that has the potential to enhance nutrient cycling in agricultural systems for sustainable development. In this study, four experiments were conducted to compare the Department of Agriculture (DOA, Thailand)’s methodology for determining the suitable type and rate of animal manure and the optimal light intensity for the growth and yield of Azolla (Azolla microphylla). The results revealed that applying 100% pig manure gave the highest yield of Azolla compared to the other manures. However, there was no discernible (p > 0.05) difference in yield across the various doses (20.16, 30.16, and 40.16 gN m⁻²) of pig manure treatments, for which the minimal pig manure dosage of 20.16 gN m⁻² was chosen. For further experimentation in the optimal light intensity, the 40% shading gave the highest yield of Azolla compared to no shading or 20 and 60% shading (p ≤ 0.01). When compared with the DOA Thailand methodology (1.27 kg m⁻² of cow manure and covered with a size 32 mesh net), the findings indicated that the modified method (20.16 gN m⁻² of pig manure + 40% shading) gave a 16% greater Azolla yield than that under the DOA Thailand methodology. The current finding method can produce a monthly fresh biomass of A. microphylla of 40.7 t ha⁻¹ year⁻¹ with higher contents of total N (4.92%) and lower C:N ratio (≤10:1) that could release minerals relatively rapidly. Its use can be encouraged by farmers to produce their own ecofriendly biofertilizer or soil amendment for sustainable agriculture. Full article

Organic farming is a comprehensive production management system that fosters and improves the health of agroecosystems, encompassing biodiversity, biological cycles, and soil biological activity. The potato (Solanum tuberosum L.) is a crucial crop in organic farming systems, standing out as one of the most highly demanded organic products on the market. Among all crops, with potatoes, there is a very large yield gap between organic and conventional systems, attributable mainly to its intensive nutrient demands. The present review, considering the most relevant scientific literature worldwide, discusses the contemporary state of knowledge on crop nutrition and soil fertility management in organic potato crop production, analyzing the effects of animal manures, green manures, organic amendments, and biostimulants on organic potato tuber yield and quality. Overall, the main findings show a particular combination is needed to effectively maintain good soil fertility, satisfy the nutritional needs of the crop, and overcome the difference in potato yield between organic and conventional farming methods while meeting consumer demand. This combination entails using an animal manure or leguminous green manure with an organic soil amendment, and even better with a biofertilizer, such as a mycorrhizae-fungus-based one. It also emerged that more targeted studies are needed to select appropriate cultivars for organic potato farming systems to optimize this environmentally friendly production method. Full article

This study addresses the challenge of finding novel ways to solubilize phosphorus and zinc for agricultural purposes. The aim was to isolate PSMs (phosphorous-solubilizing microbes) and ZnSMs (zinc-solubilizing microbes) from different environments (e.g., soil amendments, land uses, and crop rotation systems) and evaluate their ability to solubilize different insoluble P sources (e.g., β-tricalcium phosphate (β-TCP), calcium-phytate (CaP), and rock phosphate (RP)) and Zn sources (e.g., zinc carbonate (ZnC), zinc oxide (ZnO), and zinc phosphate (ZnP)). Here, 25 isolates capable of solubilizing either P or Zn sources were isolated and classified by species using 16S rRNA and ITS-region sequencing. Notably, Aspergillus awamori, Fusarium circinatum, Fusarium longifundum, and Mucor circinelloides, isolated from cultivated soils and soil amendments, emerged as the most efficient PSMs and ZnSMs. Mucor circinelloides exhibited the highest solubilization ability for broths containing β-TCP, CaP, RP, ZnO, and ZnP, with log2-fold changes of 3.7, 1.8, 8.9, 7.8, and 2.4, respectively, compared to the control. For ZnC and ZnO, Aspergillus awamori displayed the highest Zn solubilization, with a 2.1 and 3.0 log2-fold change. The study highlights the potential of these strains as biofertilizers and underscores the role of Mucor and Fusarium genera in zinc solubilization. Full article

Phosphorous (P) is widely used in agriculture; yet, P fertilizers are a nonrenewable resource. Thus, mechanisms to improve soil P bioavailability need to be found. Legumes are efficient in P acquisition and, therefore, could be used to develop new technologies to improve soil P bioavailability. Here, we studied different species and varieties of legumes and their rhizosphere microbiome responses to low-P stress. Some varieties of common beans, cowpeas, and peas displayed a similar biomass with and without P fertilization. The rhizosphere microbiome of those varieties grown without P was composed of unique microbes displaying different levels of P solubilization and mineralization. When those varieties were amended with P, some of the microbes involved in P solubilization and mineralization decreased in abundance, but other microbes were insensitive to P fertilization. The microbes that decreased in abundance upon P fertilization belonged to groups that are commonly used as biofertilizers such as Pseudomonas and Azospirillum. The microbes that were not affected by P fertilization constitute unique species involved in P mineralization such as Arenimonas daejeonensis, Hyphomicrobium hollandicum, Paenibacillus oenotherae, and Microlunatus speluncae. These P-insensitive microbes could be used to optimize P utilization and drive future sustainable agricultural practices to reduce human dependency on a nonrenewable resource. Full article

Current agriculture faces the challenge of producing food with the least interference from the environment. In this sense, the implementation of ecological agricultural practices is essential to obtaining healthy and more sustainable production systems. The objective of this study was to investigate the impact of different inoculation technologies on formulations of bokashi-type biofertilizer and its application as soil and substrate organic amendment. We examined the effects of treatments on the chemical and biological quality of the evaluated formulations, as well as their influence on the agronomic and nutritional characteristics of red-beet and cabbage crops in the field. The metagenomic analysis of the taxonomic profile of the microbiological populations revealed relative abundance of plant growth-promoting rhizobacterial genera, including Azospirillum sp., Rhizobium sp., Bradyrhizobium sp., Burkholderia sp., Paraburkholderia sp., and Paenibacillus sp. in the evaluated formulations. Additionally, no phytopathogenic contaminants were detected among the investigated treatments. The highest yields of field-grown beet crops were obtained from seedlings produced using the following treatments: bokashi biofertilizer + biodynamic preparations P502-P507, bokashi + Chamomilla 12 CH, and bokashi + Carbo vegetabilis 12 CH. The increase in productivity of cabbage plants was higher in the treatment bokashi + Calcarea carbonica 12 CH. Full article

Biofertilizer as an amendment has growing awareness. Little attention has been paid to bioremediation potential of indigenous heavy-metal-resistant microbes, especially when isolated from long-term polluted soil, as a bioinoculant in biofertilizers. Biofertilizers are a type of versatile nutrient provider and soil conditioner that is cost-competitive and highly efficient with nondisruptive detoxifying capability. Herein, we investigated the effect of biofertilizers containing indigenous cadmium (Cd)-resistant microbial consortia on rice growth and physiological response. The Thai rice cultivar PSL2 (Oryza sativa L.) was grown in Cd-enriched soils amended with 3% biofertilizer. The composition of the biofertilizers’ bacterial community at different taxonomic levels was explored using 16S rRNA gene Illumina MiSeq sequencing. Upon Cd stress, the test biofertilizer had maximum mitigating effects as shown by modulating photosynthetic pigment, MDA and proline content and enzymatic antioxidants, thereby allowing increased shoot and root biomass (46% and 53%, respectively) and reduced grain Cd content, as compared to the control. These phenomena might be attributed to increased soil pH and organic matter, as well as enriched beneficial detoxifiers, i.e., Bacteroidetes, Firmicutes and Proteobacteria, in the biofertilizers. The test biofertilizer was effective in alleviating Cd stress by improving soil biophysicochemical traits to limit Cd bioavailability, along with adjusting physiological traits such as antioxidative defense. This study first demonstrated that incorporating biofertilizer derived from indigenous Cd-resistant microbes could restrict Cd contents and consequently enhance plant growth and tolerance in polluted soil. Full article