Search Results (2,042)

Recycled cellulosic fiber (RCF) composites offer significant potential to reduce environmental burdens associated with virgin fiber production; however, their broader adoption remains limited by feedstock variability, recycling-induced degradation, and uncertainty regarding long-term performance. This review critically synthesizes recent advances in RCF composites using a structure–processing–performance–sustainability framework, treating recycled fibers as secondary materials with distinct morphological, chemical, and mechanical characteristics rather than direct substitutes for virgin reinforcements. Emphasis is placed on the effects of fiber shortening, surface damage, moisture sensitivity, and altered surface chemistry on interfacial adhesion, load transfer efficiency, durability, and failure mechanisms. The analysis reveals that many reported performance discrepancies arise from poorly defined structure–property relationships and the absence of standardized characterization, grading, and durability testing protocols for recycled fibers. Addressing these gaps enables more reliable predictive modeling and application-specific material design. Beyond mechanical behavior, the review evaluates various critical factors for integration into higher-value applications such as durability under realistic service conditions, including environmental aging, fire performance, and long-term stability. Emerging strategies such as hybrid reinforcement, environmentally benign surface functionalization, smart functionalities, and recyclable or bio-based matrices are assessed for their potential to enhance multifunctionality and circularity. Overall, the findings indicate that RCF composites can meaningfully contribute to circular material systems if materials design, performance validation, and life-cycle assessment are integrated systematically. Advancing standardized evaluation and aligning materials innovation with circular economy principles are essential to transition RCF composites from downcycled applications to reliable, performance-oriented components in sustainable engineering systems. Full article

Background: The constant growth in demand for sustainable energy products and the development of the circular economy have created a critical need for an efficient supply chain for biomass. However, the inherent challenges of biomass make its harvesting, collection, storage, and transport difficult, impacting logistical efficiency and the viability of bioenergy and bioproduct production. This study analyzes how combining artificial intelligence (AI) with multimodal transport can optimize and improve efficiency, as well as reduce costs, in biomass logistics. Methods: The study uses a tiered research framework that encompasses the physical domain (biomass limitations), the structural domain (mathematical modeling for multimodal transport), the intelligence domain (AI-based decision making), and the strategic approach. Results: The outcomes indicate that while truck transport is ideal for short distances, integrating rail and water transport through AI-driven optimization reduces costs and greenhouse gas emissions for long-distance travel. AI technologies, such as digital twins and machine learning, improve demand forecasting, real-time routing, and cargo consolidation, leading to enhanced prediction accuracy for transport costs. Conclusions: The integration of AI and multimodal networks builds resilient and sustainable biomass supply chains. However, full implementation requires addressing data fragmentation and investing in digital infrastructure to enable seamless coordination between supply chain stakeholders. Full article

Objectives: While several physiological functions of milt peptides have been discovered, the structural characteristics of Theragra chalcogramma milt peptides (TMP) and their anti-fatigue mechanisms remain unclear. Methods: TMP was obtained by hydrolysis via flavor enzyme and alkaline protease, and its structural characteristics were analyzed. A mice model of exercise-induced fatigue was established. The anti-fatigue effect of TMP was evaluated by determining the main biochemical indices in the serum, liver, and skeletal muscle of mice. Additionally, qPCR analysis was conducted to investigate its regulatory effects on relevant energy metabolism pathways. Results: TMP contained 18.2% branched-chain amino acids, with those with molecular weights below 1000 Da accounting for 91.6%. A total of 154 characteristic peptides, such as VPFPR and LPPGR, were identified from TMP, among which 64% of the peptides contained glutamic acid, arginine, or aspartic acid. Molecular docking of potential bioactive peptides to AMP-activated protein kinase (AMPK) revealed binding energies from −9.1 to −5.5 kcal/mol. The exhaustive swimming test showed that oral administration of TMP prolonged the swimming duration. In the fatigue murine model, TMP reduced blood urea nitrogen and blood lactic acid levels while enhancing the content of muscle glycogen. Meanwhile, TMP significantly increased the activity of glutathione peroxidase and superoxide dismutase and reduced the accumulation of malondialdehyde, demonstrating antioxidant properties. Additionally, TMP significantly decreased creatine kinase and lactate dehydrogenase extravasation, thereby protecting muscle tissue, as corroborated by immunohistochemical analyses. Mechanistically, TMP upregulated AMPK and peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) expression, promoting mitochondrial biogenesis via the AMPK/PGC-1α pathway. Conclusions: These findings suggest TMP has potential as a dietary supplement for alleviating physical fatigue. Full article

Background: Medicinal plants function as complex holobionts, with their therapeutic potential significantly shaped by the associated microbiome, particularly endophytic fungi. These symbionts engage in a sophisticated “chemical signaling” with their hosts, acting as biotic elicitors that modulate plant secondary metabolism while simultaneously responding to host cues to activate their own cryptic biosynthetic gene clusters (BGCs). This review aims to critically summarize the multi-layered mechanisms driving this metabolic crosstalk and evaluate strategies to harness this symbiotic intelligence for natural product discovery. Methods: A systematic literature survey spanning the last decade was conducted across major databases. The search specifically targeted studies investigating endophytic fungi in medicinal plants, focusing on experimental designs for BGC activation, applications of spatial metabolomics (matrix-assisted laser desorption/ionization mass spectrometry imaging, MALDI-MSI), and the structural elucidation of novel bioactive natural products through co-culture or in planta models. Results: Our analysis reveals that host-derived chemical cues, such as specific root exudates and oxylipins, act as primary triggers to awaken silent fungal BGCs. We collated numerous recently discovered bioactive metabolites—including novel polyketides, highly rearranged terpenoids, and unique alkaloids—demonstrating their potent antimicrobial and cytotoxic properties. Furthermore, a critical evaluation of spatial metabolomics studies demonstrates that metabolic exchange is highly localized at the plant–fungus interface, providing contextual insights that traditional bulk tissue extraction fails to capture. Conclusions: This review bridges the gap between ecological understanding and synthetic biology applications. We conclude that translating the mechanisms of this “chemical signaling” into biotechnological strategies offers a sustainable pathway for the bioproduction of high-value pharmaceuticals, thereby reducing reliance on the wild harvesting of medicinal plants. Full article

Grapevine trunk diseases, particularly Botryosphaeria dieback, pose a major threat to vineyard sustainability, a risk that is further intensified by climate variability and increasing environmental stress. This study evaluated pathogenicity and bacterial biocontrol efficacy against Neofusicoccum parvum and Diplodia seriata under vineyard conditions, analyzing the combined effects of cultivar (Vitis vinifera L. cv. Cabernet Sauvignon and Sauvignon Blanc), tissue type (young shoots and lignified arms), and phenological season (autumn/winter and spring/summer). Pathogenicity assays revealed clear tissue-age specialization: N. parvum was more aggressive in young shoots, whereas D. seriata caused the most severe vascular lesions in lignified wood. Seasonality further modulated disease expression, with higher lesion development during spring/summer, particularly for N. parvum in young shoots, while D. seriata maintained high aggressiveness in lignified tissues across both seasons. Berry assays provided a rapid initial assessment of isolate virulence but did not fully reflect pathogen behavior in woody tissue under field conditions. Biological treatments using native bacterial strains (Pseudomonas sp. AMCR2b, GcR15a, and Rhodococcus sp. PU4) significantly reduced lesion severity in V. vinifera under field conditions, although efficacy varied by tissue type and season. Biocontrol effects were generally more stable in lignified arms, and under high disease pressure, only the most robust strains maintained consistent protection, in some cases matching or surpassing the efficacy of the fungicide tebuconazole. These results show that both pathogenicity and biocontrol performance against Botryosphaeria dieback in V. vinifera under field conditions are strongly influenced by tissue type and season, supporting bacterial biocontrol as a sustainable component of integrated disease management in vineyards. Full article

Breast cancer continues to be a major challenge in global health, in part due to significant inequalities in access to costly diagnostic and therapeutic technologies based on antibodies. Their manufacturing requires complex and expensive bioproduction systems, resulting in limited availability of these tools—essential for early detection and targeted treatment—in many regions, particularly in Latin America. This gap has highlighted the need for cost-effective and scalable theranostic alternatives, increasing interest in aptamers. Obtained through SELEX technology, aptamers are synthetic DNA or RNA oligomers that fold into functional structures. Among their advantages are high affinity for their target, low immunogenicity, and chemical synthesis, which assures reproducible production. Aptamers have expanded the landscape of diagnostic platforms through the development of sensitive aptasensors, liquid biopsy strategies, and imaging systems based on nanomedicines. They also contribute to targeted therapy by recognizing cancer biomarkers selectively and enabling controlled drug delivery. This review presents a critical summary of advances in aptamer-based theranostics for breast cancer, addressing molecular mechanisms, structural folding, selective ligand binding, and nanomaterial interfacing. We also discuss applications in extracellular vesicle capture, cancer stem cell detection, and therapeutic conjugates, emphasizing their advantages and limitations relative to approaches based on antibodies. Overall, current advances show aptamers as emerging tools capable of democratizing precision oncology, particularly in regions where access to advanced technologies remains limited. Full article

Wood remains a cornerstone material in construction and outdoor applications, yet its durability is continually compromised by fungal decay and insect infestation. Increasing regulatory restrictions on conventional wood preservatives and growing sustainability demands have intensified interest in bio-based alternatives. Among these, essential oils exhibit strong antifungal and insect-repellent activity but suffer from high volatility, leaching, and limited durability under moisture exposure. This review examines recent advances in chitosan nanoparticle-based encapsulation of essential oils as a strategy to overcome these limitations and enable more sustainable and environmentally responsible wood protection systems. The review synthesizes current knowledge on nanoparticle synthesis routes, physicochemical properties, bioactive delivery mechanisms, antifungal and anti-termite performance, and behavior under moisture and weathering conditions, alongside sustainability and regulatory considerations. The reviewed literature demonstrates that chitosan nanoparticles enhance essential oil retention, stability, and controlled release, leading to improved resistance against biological deterioration compared with unencapsulated formulations. In addition to performance benefits, these nano-enabled systems align with circular bioeconomy principles by utilizing renewable and waste-derived feedstocks while avoiding heavy metals and persistent synthetic biocides. Despite promising laboratory results, challenges remain related to long-term field performance, scalability, and environmental fate. Overall, chitosan–essential oil nano-formulations represent a versatile platform for next-generation, low-hazard wood protection, offering a promising pathway toward sustainable and durable wood preservation technologies. Full article

Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory respiratory disorder with a globally increasing prevalence. Current therapeutic strategies are limited by drug resistance and safety concerns. Studies suggest that inhibiting the secretion of inflammatory cytokines represents a promising approach for COPD treatment. Phosphodiesterase-4 (PDE4) inhibitors have emerged as potent anti-inflammatory agents for respiratory diseases. In this study, we integrated a marine-derived natural product with computer-aided drug design to develop 32 novel PDE4 inhibitors. Compound B7 exhibited potent PDE4 inhibitory activity and a favorable safety profile. In rat model of COPD, B7 significantly reduced inflammatory cell infiltration and cytokine levels, ameliorated pathological changes in the lung, decreased the percentage of goblet cell positivity, and reduced expiratory resistance. Furthermore, in vitro mechanistic studies revealed that B7 exerts its anti-inflammatory effects by activating the cAMP-PKA-CREB signaling pathway and suppressing the NF-κB pathway in RAW264.7 cells. In conclusion, B7 demonstrates potential as a safe and effective PDE4-targeted candidate for the treatment of COPD. Full article

Lignocellulose biomass (LB) has gained interest as a second-generation renewable feedstock for producing bio-based products within a circular economy framework. Hemp hurds, a byproduct of industrial hemp processing, are one of the LB feedstocks that have gained attraction. This study examines the physicochemical properties of hemp hurds to evaluate their suitability as substrates for bioproduct synthesis. The chemical analysis of hemp hurds showed that the polysaccharide content is 53.4%, lignin is 20.8%, extractives are 15%, and ash is 4.35%. The moisture content is 6.34%, and the density is 1.0016 g/mL, indicating low porosity and a small surface area, which limits enzyme access to cellulose. Structural analysis using X-ray diffraction (XRD) indicated a crystallinity index of 40.20%, and the Fourier Transform Infrared Spectrophotometer (FTIR) confirmed the characteristic peaks representing cellulose, hemicellulose, and lignin at 3332 cm⁻¹, 1734 cm⁻¹, and 1510 cm⁻¹, respectively. The Scanning Electron Microscope (SEM) revealed a tightly packed surface with smooth, low porosity, whereas the Thermogravimetric Analyser (TGA) indicated decomposition in phases for hemicellulose, cellulose, and lignin. The structural and chemical findings of hemp hurds characterisation suggest that they are a suitable raw material for producing various bio-based materials. Full article

Asparagopsis has gained global attention for its chemical properties and environmental applications. However, its two main species, Asparagopsis armata and Asparagopsis taxiformis, remain understudied, with limited information available regarding their bioactive potential, especially across their development. In this study, we examined the phenolic profiles and antioxidant potentials of gametophyte and tetrasporophyte life stages and compared differences between conventional solvent extraction (CSE) and ultrasound-assisted extraction (UAE), including total phenol content, total flavonoid content, determination of condensed tannins, and seven types of antioxidant activity detections such as DPPH and ABTS. In general, the phenolic compounds and antioxidant potential of the Asparagopsis species vary significantly at different life stages and under different extraction techniques. Among them, the phenolic profile and antioxidant capacity of A. armata were recorded as significantly higher than those of A. taxiformis, as reflected by its greater relative antioxidant capacity index scores. In our study, while UAE did not universally outperform CSE, species- and life stage-specific improvements were recorded. Moreover, LC-ESI-QTOF-MS/MS tentatively identified 24 phenolic compounds (17 in A. armata and 14 in A. taxiformis), pointing to a diverse bioactive profile. Overall, Asparagopsis species demonstrated marked variability in phenolic and antioxidant potentials across life stages and extraction techniques. Full article

A novel co-encapsulation platform based on curcumin-loaded liposomes (Cur-Lip) incorporated into thermosensitive hydrogels (TSH) was developed to address the physicochemical and biological limitations of topical curcumin (Cur) delivery. Response Surface Methodology (RSM) was used to optimize Pluronic^® F-127, glycerol, and alginate concentrations with respect to gelation time and viscosity. The optimized formulation (22% Pluronic^® F-127, 5% glycerol, and 0.5% alginate) exhibited rapid time sol–gel transition (~86 s), suitable viscosity (~377 mPa·s), excellent model fitting (R² = 0.99) and prediction accuracy. Three formulations (TSH, Cur-TSH, and Cur-Lip-TSH) were subsequently prepared and displayed appropriate thermoresponsive behavior. The Cur-Lip system showed high encapsulation efficiency (~78%). Upon incorporation into the TSH, Cur-Lip-TSH displayed increased viscosity and mechanical strength at physiological temperature. In vitro studies confirmed its cytocompatibility toward human keratinocytes, significant antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa, and no irritation potential as assessed by the Hen’s Egg Test on the Chorioallantoic Membrane assay (HET-CAM). Overall, Cur-Lip-TSH represents a safe and robust thermosensitive platform that provides a foundation for future studies on controlled curcumin release and topical performance. Full article

Type 2 diabetes mellitus (T2DM) is one of the most prevalent chronic metabolic diseases, and inhibition of α-glucosidase activity represents an effective therapeutic strategy. Chitin is the most abundant renewable polysaccharide in the ocean, with its monosaccharide being N-acetylglucosamine (NAG). To evaluate the potential of NAG glycosides as novel α-glucosidase inhibitors, three common phenolic compounds were modified via NAG glycosylation. Their inhibitory activities were assessed at both the enzymatic and cellular levels. In addition, density functional theory (DFT), molecular dynamics (MD) simulations, and molecular docking analyses were employed to systematically investigate the effects of NAG glycosylation on enzyme inhibition and the underlying mechanisms. Compared with the parent phenolic compounds, NAG glycosides exhibited significantly enhanced α-glucosidase inhibitory activity, with NAG introduction markedly improving their binding affinity to α-glucosidase. Among them, glycoside 3a displayed the optimal inhibitory effect, comparable to acarbose, and at the cellular level, its activity at high concentrations was comparable to or slightly higher than that of metformin. Circular dichroism (CD) and MD analyses indicated that glycoside 3a increased the conformational flexibility of key residues and enhanced the structural looseness of the enzyme, thereby inhibiting its activity. NAG glycosides constitute a promising class of marine-derived α-glucosidase inhibitors, warranting further structural optimization and rational design to enhance their activity and selectivity. Full article

The valorization of agro-industrial residues through fermentation processes represents a sustainable approach to producing high-value bioproducts, such as microbial organic acids and fermentation-derived anti-browning agents, including kojic acid and kojic acid-rich fermented extracts. In this study, melon waste (non-commercial-quality or damaged fruit) was evaluated as an alternative carbon source (whole fruit) for kojic acid (KA) production by Aspergillus oryzae (ATCC 10124) under submerged fermentation. The effects of process variables such as pH, temperature, and nitrogen and carbon availability on KA synthesis were analyzed, and biomass growth and product formation were described using logistic and Luedeking–Piret kinetic models. Under optimal conditions (pH 5.5, 36 °C, 2.5 g/L melon dry matter, 2.5 g/L yeast extract, 100 rpm), KA production reached 1.64 g/L at a final time of 120 h. Kinetic analysis showed moderate fungal growth (μ_max = 0.058 h⁻¹; X_max = 0.81 g/L), with KA formation following a mixed growth-associated pattern as described by the Luedeking–Piret model (α = 1.26 g KA/g X; β = 0.024 h⁻¹), indicating sustained production during the stationary phase. The KA-rich fermented extract was subsequently applied as an anti-browning treatment on spineless prickly pear (Opuntia ficus-indica) cladodes. Short immersion times (0.5–1.0 min) in a 2 g/L KA solution significantly preserved luminosity (L*) and limited total color change (ΔE ≤ 5) during 4 days of storage at 28 °C, compared with water-treated controls, which exhibited accelerated darkening (ΔE ≈ 9–15). Prolonged immersion times induced tissue damage and color deterioration, indicating an optimal exposure window. These results demonstrate the feasibility of valorizing melon waste to obtain a KA-rich extract and support its potential application as a natural anti-browning agent in fresh-cut vegetables within a circular agrifood framework. Full article

The widespread application of road deicing salts in northern regions has led to elevated salinity in roadside soils and adjacent watersheds. Phytoremediation offers a cost-effective and sustainable approach for mitigating salt contamination, but its success depends on utilizing plant species that can both tolerate and remove salt under roadside conditions. To systematically identify high-potential candidates from the large inventory of salt-tolerant plants in North America, we developed a quantitative decision matrix incorporating criteria related to ecological safety, establishment potential on disturbed soils, aboveground biomass production, biomass use-value, and salt uptake capacity. Thirteen of the highest-ranked species were subsequently evaluated for sodium (Na⁺) and chloride (Cl⁻) uptake in a controlled greenhouse study under saline and non-saline conditions. The greatest total salt uptake was observed in common sunflower (Helianthus annuus) (35.6 mg Na⁺ and 100.2 mg Cl⁻ plant⁻¹) and pitseed goosefoot (Chenopodium berlandieri) (18.6 mg Na⁺ and 76.0 mg Cl⁻ plant⁻¹), while perennial species including tall fescue turfgrass (Lolium arundinaceum), showy goldenrod (Solidago speciosa), and weeping alkaligrass (Puccinellia distans) also demonstrated substantial uptake combined with greater long-term suitability for roadside management. Overall, this study presents a quantitative framework for phytoremediation species selection and provides experimental evidence supporting both annual and perennial species for mitigating deicing salt contamination through environmentally sustainable, low-input roadside management strategies. Full article

Phaeodactylum tricornutum is one of the most well-characterized microalgae and serves as a pivotal model diatom in global carbon fixation and the mediation of biogeochemical cycling of essential nutrients. Over the past few decades, the availability of a complete genome assembly, coupled with the development of robust DNA manipulation tools and efficient DNA delivery methodologies, has established P. tricornutum as a promising photosynthetic chassis for the sustainable bioproduction of high-value compounds, including fucoxanthin and eicosapentaenoic acid (EPA). This review systematically summarizes the research progress in the strain improvement toolkit of P. tricornutum, encompassing both genetic and non-genetic engineering strategies. It elaborates on the types and applications of its representative bioactive products, as well as the molecular mechanisms underlying key synthetic pathways. Additionally, this work synthesizes the research findings on the optimization of critical cultivation conditions (e.g., light, temperature, and nutrient composition) that modulate the growth and product synthesis of P. tricornutum. On this basis, the challenges encountered by P. tricornutum in industrial applications are proposed for further discussion, aiming to provide a reference for in-depth exploration of related research directions and facilitate the expansion of its application scope in the field of biomanufacturing. Full article