Search Results (1,202)

Following restrictions on antibiotic growth promoters in poultry production, there is growing interest in natural feed additives that support health and productivity. Among these, black cumin (Nigella sativa) has emerged as a promising candidate due to its antioxidant, antimicrobial, and immunomodulatory properties. Most studies report that black cumin, in the form of whole seeds, seed meal, or seed oil, improves body weight gain and feed conversion ratio, enhances antioxidant and immune status, and provides additional benefits on lipid profiles, liver enzymes, and cecal microbial balance. This review provides a focused synthesis of recent studies (2014–2025) on black cumin supplementation in broiler chickens, considering its various forms (whole seeds, seed meal, seed oil, and nano-formulations) and production contexts (healthy, heat-stressed, and disease-challenged birds). Specifically, this review compares responses across different forms and doses, evaluates effects on growth performance, immune function, gut health, antioxidant status, liver metabolism, and meat and carcass quality, and highlights inconsistencies among studies. Additionally, it identifies key research gaps to guide future investigations, including optimal dosing, long-term safety, and practical applications in commercial production. Overall, black cumin shows potential as a natural alternative to antibiotics, but further standardized, large-scale studies are needed to confirm its efficacy and feasibility in sustainable poultry farming. Full article

Restrictions on adding antibiotics to animal diets have posed challenges in managing gut pathogens, emphasizing the significance of effective non-antibiotic growth promoters to maintain animal health and productivity. This study assessed the efficacy of hydroxamic acid (HA), derived from local maize varieties, as a non-antibiotic growth promoter in broilers. Among 10 different maize varieties, the Azam variety yielded the highest HA concentration (35 ± 7 μg/g of roots), as quantified by high-performance liquid chromatography (HPLC). In vitro antimicrobial assays demonstrated the lowest minimum inhibitory concentration (MIC) of 0.022 mg for Azam-derived HA against pathogenic E. coli. To further assess in vivo efficacy, 108 birds were allocated at random to six treatment groups. The treatments include birds fed a basal diet without an E. coli challenge (negative control); an antibiotic-treated group challenged with E. coli and treated with enrofloxacin at a dosage of 5 milligrams (mg) per kilogram (kg), administered orally once daily from day 5 post-infection (dpi) for 7 consecutive days (standard); broilers challenged with E. coli and supplemented with a basal diet with HA at concentrations of 1, 10, or 100 mg/kg of feed from 5 dpi for one week (HA 1 mg, HA 10 mg, and HA 100 mg, respectively); and broilers challenged with E. coli without enrofloxacin/HA (positive control). The results demonstrated that birds fed a diet supplemented with the HA-100 mg improved the body weight (BW) and feed conversion ratio (FCR) compared to the positive control group. There were no significant differences (p > 0.05) observed for BW and FCR observed for the broilers fed on the standard and HA 100 mg groups. The addition of HA at 100 mg improved (p < 0.05) the hemoglobin (Hb) and packed cell volume (PCV) and reduced (p < 0.05) levels of malondialdehyde (MDA) compared to positive control group. A significantly low carcass weight (p < 0.05) was shown for positive control birds compared to other groups. Our findings indicate that maize-derived HA presents a phytogenic alternative to antibiotics by controlling enteric pathogens and improving health and performance affected by E. coli infection in broilers. Full article

Simulation-based studies can support breeders’ decisions inexpensively, since there is no need to perform a new procedure. The objective was to assess the efficiency of recurrent genomic selection in panmictic population under additive–dominance and additive–dominance with epistasis models. We assumed two broiler chicken populations with contrasting linkage disequilibrium (LD) levels, 38,500 SNPs, and 1000 genes controlling feed conversion ratio. We applied recurrent genomic selection over seven cycles. The genomic selection efficacy, expressed as realized total genetic gain, was proportional to the LD level and genotypic variance. Genomic selection required model updating to achieve a higher efficacy. The training set size required by genomic selection can be as low as 10%/generation. Under this low-cost scenario, the genomic selection efficacy was slightly lower than the maximum efficacy. There is no difference between genetic evaluation methods regarding the decrease in the genotypic variance due to selection. In general, additive value prediction accuracies and realized genetic gains were highly correlated. The accumulated inbreeding level was not high due to avoidance of sib cross. The genomic inbreeding coefficient over generations was close to zero. Except for dominant epistasis, the efficacy of genomic selection was 4.1 to 46.2% lower than the efficacy under no epistasis. Full article

Reliable individual detection under dense and cluttered conditions is a prerequisite for automated monitoring in modern poultry systems. We propose CF-DETR, an end-to-end detector that builds on RT-DETR and is tailored to chicken face detection in production-like environments. CF-DETR advances three technical directions: Dynamic Inception Depthwise Convolution (DIDC) expands directional and multi-scale receptive fields while remaining lightweight, Polar Embedded Multi-Scale Encoder (PEMD) restores global context and fuses multi-scale information to compensate for lost high-frequency details, and a Matchability Aware Loss (MAL) aligns predicted confidence with localization quality to accelerate convergence and improve discrimination. On a comprehensive broiler dataset, CF-DETR achieves a mean average precision at IoU 0.50 of 96.9% and a mean average precision (IoU 0.50–0.95) of 62.8%. Compared to the RT-DETR baseline, CF-DETR reduces trainable parameters by 33.2% and lowers FLOPs by 23.0% while achieving 81.4 frames per second. Ablation studies confirm that each module contributes to performance gains and that the combined design materially enhances robustness to occlusion and background clutter. Owing to its lightweight design, CF-DETR is well-suited for deployment in real-time smart farming monitoring systems. These results indicate that CF-DETR delivers an improved trade-off between detection performance and computational cost for real-time visual monitoring in intensive poultry production. Full article

This study evaluated the antibacterial efficacy and growth-promoting potential of Moringa oleifera and Thymus vulgaris methanolic extracts in broiler chickens challenged with Escherichia coli O78. In vitro antibacterial screening using agar well diffusion and disc diffusion assays revealed that ciprofloxacin exhibited the strongest inhibitory effect, followed by tetracycline and enrofloxacin, whereas among plant extracts, T. vulgaris was more effective than M. oleifera. The optimal combination (M100T150; 100 mg M. oleifera + 150 mg T. vulgaris) produced the largest inhibition zones against E. coli strains. For the in vivo trial, 540 Ross-308 broiler chicks were distributed into six treatment groups in a completely randomized design and reared for 42 days. Parameters assessed included growth performance, carcass traits, gut pH, ileal microbial counts, and intestinal histomorphology. Results showed that E. coli challenge significantly reduced feed intake, weight gain, carcass yield, and villus integrity while increasing FCR and E. coli counts (p < 0.05). Addition of plant extracts, particularly M100T150, significantly improved weight gain, FCR, Broiler Performance Efficiency Factor (BPEF), and Broiler Farm Economy Index (BFEI) compared to the positive control (p < 0.05). Extracts reduced duodenal and jejunal pH (p < 0.001), suppressed E. coli counts (p = 0.003), and enhanced Lactobacillus populations (p = 0.0004). Histological analysis revealed that extract-supplemented groups had greater villus height and surface area with shallower crypts than the positive control, indicating restoration of gut integrity. These findings suggest that methanolic extracts of M. oleifera and T. vulgaris, particularly in combination, can serve as natural alternatives to antibiotics in broiler production under pathogenic challenge. Full article

Broiler chickens are commonly reared in closed housing systems with limited exposure to sunlight, thereby relying entirely on dietary sources of vitamin D. The hydroxylated metabolite 25-hydroxycholecalciferol [25(OH)D₃] has been proposed as a more potent form than native vitamin D₃ (cholecalciferol). This study evaluated the effects of dietary supplementation with vitamin D₃ alone or in combination with 25(OH)D₃ on growth performance, bone characteristics, and cecal microbiota in Ross 308 broilers. A total of 952 one-day-old male chicks were allocated to four treatments: a negative control (no vitamin D₃), a positive control (vitamin D₃ according to Ross 308 specifications), and a positive control supplemented with 25(OH)D₃ at 1394 or 2788 IU/kg, in a randomized design with 17 replicates per treatment and 14 birds per replicate. Over a 40-day feeding trial, diets containing vitamin D₃ (positive control) or supplemented with 25(OH)D₃ significantly improved final body weight, weight gain, average daily gain, and feed conversion ratio compared with the negative control (p < 0.01), with no significant differences among the positive control and 25(OH)D₃-supplemented groups, with a clear linear dose-dependent response. Although tibia ash and bone-breaking strength were not significantly affected, linear responses indicated a slight numerical trend toward improved skeletal mineralization with increasing 25(OH)D₃. Microbiota analysis indicated that 25(OH)D₃ affected cecal microbial ecology: low-dose inclusion showed reduced species richness and evenness, whereas high-dose inclusion restored richness to levels comparable to the positive control and enriched taxa associated with fiber fermentation and bile acid metabolism while reducing Lactobacillus dominance. In conclusion, supplementation with 25(OH)D₃ in addition to vitamin D₃ enhanced growth performance and selectively shaped the cecal microbiota of broilers, with suggestive benefits for bone mineralization. These findings highlight 25(OH)D₃ as a more potent source of vitamin D than cholecalciferol alone and support its practical use in modern broiler nutrition to improve efficiency, skeletal health, and microbial balance. Full article

The aim of the present study is to evaluate the impact of chromium (Cr) supplementation on glucose and lipid metabolism in breast muscle in broilers under heat stress. A total of 220 day-old broiler chicks were reared in cages. At 29 days old, 180 birds were randomly assigned to three treatments (0, 400, and 800 µg Cr/kg, as chromium picolinate) and transferred to climate chambers (31 ± 1 °C, 60 ± 7% humidity) for 14 days. Growth performance, carcass traits, serum biochemical indices, fasting glucose and insulin, homeostasis model assessment of insulin resistance (HOMA-IR), as well as muscle metabolomic profiles and gene expression related to energy and lipid metabolism were analyzed. The results showed that, compared with the heat stress group, the groups supplemented with 400 and 800 µg Cr/kg showed higher dry matter intake and average daily gain, breast muscle ratio, and lower feed conversion ratio and abdominal fat ratio; chickens supplemented with 400 and 800 µg Cr/kg showed significantly lower serum corticosterone (CORT), free fatty acids, and cholesterol levels compared with the heat stress (HS) group (p < 0.05). Fasting blood glucose and HOMA-IR were also significantly reduced, while fasting insulin was significantly increased in the Cr-supplemented groups (p < 0.05). Metabolomic analysis revealed that Cr supplementation regulated lipid and amino acid metabolism by altering key metabolites such as citric acid, L-glutamine, and L-proline, and modulating pathways including alanine, aspartate, and glutamate metabolism, and glycerophospholipid metabolism. Furthermore, Cr supplementation significantly upregulated the expression of Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 α (PGC-1α), ATP Binding Cassette Subfamily A Member 1 (ABCA1), Peroxisome Proliferator-Activated Receptor α (PPARα), and ATP Binding Cassette Subfamily G Member 1 (ABCG1) in both the hepatic and muscle tissue. This paper suggested that chromium supplementation may enhance energy metabolism and lipid transport like the findings of our study suggested. Full article

In intensive broiler production, rapid environmental fluctuations can induce heat stress, adversely affecting flock welfare and productivity. Apparent temperature (AT), integrating temperature, humidity, and wind speed, provides a comprehensive thermal index, guiding predictive climate control. This study develops a multi-step AT forecasting model based on a hybrid SE-TCN–Transformer architecture enhanced with Kalman filtering. The temporal convolutional network with SE attention extracts short-term local trends, the Transformer captures long-range dependencies, and Kalman smoothing reduces prediction noise, collectively improving robustness and accuracy. The model was trained on multi-source time-series data from a commercial broiler house and evaluated for 5, 15, and 30 min horizons against LSTM, GRU, Autoformer, and Informer benchmarks. Results indicate that the proposed model achieves substantially lower prediction errors and higher determination coefficients. By combining multi-variable feature integration, local–global temporal modeling, and dynamic smoothing, the model offers a precise and reliable tool for intelligent ventilation control and heat stress management. These findings provide both scientific insight into multi-step thermal environment prediction and practical guidance for optimizing broiler welfare and production performance. Full article

Environmental enrichment, such as lighting, has affected the behaviors, welfare, and production of commercial broiler chickens. However, most studies have focused on constant light intensities to determine their effect on welfare and performance. Research indicates that the significant contrast of light intensities in broiler houses promotes pronounced daily patterns of behavior and activity, impacting broiler chicken health. Birds exhibited preference behaviors in bright-intensity light during active behaviors, such as eating and drinking, but in darker areas when resting. Light intensity preferences may be associated with the voluntary instinctive movement of birds by providing choices for birds. Increasing broiler chickens’ movement may boost welfare, especially leg health, which is a leading cause of culling and late mortality in commercial production. In this review, we discuss the progress and results of practical environmental enrichments, enrichment lighting, and huts in commercial broiler houses. We briefly address interpretations of improved welfare and performance and suggest directions for future research that may interest poultry scientists. Full article

This study aimed to investigate the antioxidant properties of natural palygorskite (N-pal), bundle-dissociated palygorskite (D-pal), and zinc-bearing palygorskite (Zn-pal), as well as their effects on the antioxidant capacity of broilers. Palygorskite (Pal) and its derivatives exhibited hydrogen peroxide (H₂O₂) decomposition and hydroxyl radical (·OH) scavenging abilities, with D-pal and Zn-pal demonstrating superior performance. A total of 320 one-day-old Arbor Acres broilers were randomly divided into 4 groups: control, N-pal, D-pal and Zn-pal groups. The corn–soybean meal basal diet was supplemented with N-pal (10 g/kg), D-pal (5 g/kg) and Zn-pal (1 g/kg) for the respective treatment groups. The trial lasted for 42 days. At 21 days, D-pal and Zn-pal groups significantly increased average body weight and average daily weight gain while reducing the feed-to-gain ratio (p < 0.05). Both reactive oxygen species (ROS) and malondialdehyde (MDA) levels in liver of broilers were significantly reduced, and glutathione peroxidase 1 (GPX1) gene expression was upregulated at 21 days (p < 0.05). N-pal and D-pal groups enhanced superoxide dismutase (SOD) and catalase (CAT) activities in the duodenum (21d); D-pal group increased SOD activity in the ileum (42d); all Pal groups decreased ileal jejunal mucosal ROS (21d) and MDA (42d) (p < 0.05). At 42 days, Pal supplementation downregulated oxidative stress (N-pal), oxidoreductase activity (D-pal), and H₂O₂ response (Zn-pal) pathways in jejunal mucosa. N-pal and D-pal groups upregulated ileal mucosal nuclear factor-erythroid 2-related factor-2 (Nrf2), heme oxygenase-1 (HO-1), and superoxide dismutase 1 expression, while Zn-pal group increased HO-1 expression (21d). D-pal and Zn-pal groups enhanced jejunal mucosal Nrf2 and HO-1 expression (21d). Pal improved the antioxidant capacity in broilers by activating the Nrf2-mediated pathway, upregulating antioxidant-related gene expression, particularly at 21 days. Both D-pal and Zn-pal demonstrated potent antioxidant efficacy, and they can improve growth performance by enhancing the animal’s antioxidant capacity. Full article

This study evaluated the effects of citric acid by-product (CABP), either alone or in combination with fibrolytic enzyme (F) and Lactobacillus spp. (L), in broiler diets. A total of 160 one-day-old male Arbor Acres broiler chicks (average initial body weight 43.0 ± 0.16 g) were randomly assigned to four CABP-based diets (4 replicates × 10 birds each): 12% CABP, CABP+F (0.05%), CABP+L (0.025%), and CABP+FL (0.05% F + 0.025% L). Birds were fed for 35 days in three phases: starter (days 1–10), grower (days 11–24), and finisher (days 25–35). Compared with the CABP-only group, broilers receiving CABP+F, CABP+L, or CABP+FL exhibited higher body weight and weight gain during the finisher and overall phases (p < 0.05). Carcass traits did not differ significantly among treatments, although meat hardness was reduced in the CABP+FL group (p < 0.05). Additive-supplemented groups also showed changes in intestinal morphology, with a lower villus height-to-crypt depth ratio compared with CABP alone. Red blood cell counts were not different between CABP and CABP+L, while hepatic enzyme activities (ALT, AST, ALP) remained unchanged across treatments, indicating no adverse effects on liver function. Overall, supplementation of CABP-based diets with fibrolytic enzymes and/or Lactobacillus spp. improved broiler performance and certain meat quality traits relative to CABP alone, without negative impacts on liver health or carcass yield. However, as no conventional corn–soy control diet was included, the results should be interpreted as comparisons within CABP-based diets. Full article

Bacillus species represent promising alternatives to antimicrobial growth promoters, offering potential benefits for productivity and gut health in broilers. This study aimed to isolate Bacillus strains with inhibitory activity against Clostridium perfringens and evaluate their probiotic potential through in vitro and in vivo approaches. In Experiment 1 (in vitro), five strains—B. siamensis C66, B. tequilensis Y7, B. velezensis L15, B. amyloliquefaciens C271, and B. siamensis C377—were isolated and assessed for stress tolerance, digestive enzyme production, and antimicrobial activity. All strains demonstrated high survival rates under acid and bile stress, produced multiple digestive enzymes, and significantly inhibited the growth of C. perfringens. In Experiment 2 (in vivo), 630 day-old male Arbor Acres broilers were randomly assigned to one of seven dietary treatments for 42 days: a negative control (CON, basal diet), a positive control (ANfT, basal diet supplemented with 6.4 g/t virginiamycin), and five groups receiving basal diet supplemented with one of the Bacillus strains at 1 × 10¹¹ CFU/kg. Among these, B. amyloliquefaciens C271 significantly increased breast muscle yield (p < 0.05), improved jejunal morphology—evidenced by increased villus height and villus height-to-crypt depth ratio (p < 0.05)—and positively modulated cecal microbiota composition compared to the CON group. These findings demonstrate that the newly isolated B. amyloliquefaciens C271 possesses strong probiotic properties in vitro and promotes growth performance and gut health in broilers, suggesting its viability as an antibiotic growth promoter substitute. Full article

This study was conducted to investigate the interaction of dietary fat level and lysolecithin (LL) supplementation on the growth performance, nutrient digestibility, intestinal morphology, intestinal lipase activity, and lipid metabolism in yellow-feathered broilers (YFBs) during day 1–50. A total of 384 one-day-old male broilers were assigned to a 2 × 2 factorial design, including dietary fat level (Normal-fat, NF: 3% lard, day 1–21; 4.5% lard, day 22–50/Low-fat, LF: 2.4% lard, day 1–21; 3.6% lard, day 22–50) and LL supplementation (0% or 0.05%). Each treatment had six replicates with 16 birds each. The trial lasted 50 days from day 1 to day 50. During the starter phase (day 1–21), birds fed the NF diet exhibited significantly greater average daily gain (ADG) and a lower feed conversion ratio (FCR) compared to those fed the LF diet (p < 0.05). Supplementation with LL significantly improved ADG and reduced FCR throughout all phases (day 0–21, day 22–50, and day 0–50) (p < 0.05). At day 21, LL also increased the apparent metabolizable energy (AME) and the digestibility of crude protein and ether extract (p < 0.05). LL supplementation increased the villus height-to-crypt depth (VH:CD) ratio in the small intestine and elevated intestinal lipase activity during the experimental period (p < 0.05). Regarding lipid metabolism, LL supplementation decreased serum total cholesterol (TC) and the LDL:HDL ratio, while increasing liver total lipase (TL) activity at both day 21 and day 50 (p < 0.05). These findings suggest that dietary supplementation with 0.05% LL improves growth performance, enhances nutrient digestibility and intestinal absorptive capacity, and positively modulates lipid metabolism in YFBs. Full article

Open-mouth panting in broiler chickens is a visible and critical indicator of heat stress and compromised welfare. However, detecting this behavior in densely populated cages is challenging due to the small size of the target and frequent occlusions and cluttered backgrounds. To overcome these issues, we proposed an enhanced object detection method based on the lightweight YOLOv8n framework, incorporating four key improvements. First, we add a dedicated P2 detection head to improve the recognition of small targets. Second, a space-to-depth grouped convolution module (SGConv) is introduced to capture fine-grained texture and edge features crucial for panting identification. Third, a bidirectional feature pyramid network (BIFPN) merges multi-scale feature maps for richer representations. Finally, a squeeze-and-excitation (SE) channel attention mechanism emphasizes mouth-related cues while suppressing irrelevant background noise. We trained and evaluated the method on a comprehensive, full-cycle broiler panting dataset covering all growth stages. Experimental results show that our method significantly outperforms baseline YOLO models, achieving 0.92 mAP@50 (independent test set) and 0.927 mAP@50 (leakage-free retraining), confirming strong generalizability while maintaining real-time performance. The initial evaluation had data partitioning limitations; method generalizability is now dually validated through both independent testing and rigorous split-then-augment retraining. This approach provides a practical tool for intelligent broiler welfare monitoring and heat stress management, contributing to improved environmental control and animal well-being. Full article

Aflatoxin contamination of animal feeds may impact broiler chicken health and production. The adverse impact of aflatoxin can be ameliorated and detoxified by adding capable binding agents, such as Saccharomyces cerevisiae. A total of 648 mixed gender 1-day-old Ross-308 were assigned to a 3 × 2 factorial experiment to investigate the effect of aflatoxin B1 (AF) and Saccharomyces cerevisiae (SAC) on growth performance, blood parameters and carcass characteristics. Chickens were randomly allocated to dietary treatments consisting of three levels of AF at 0, 20 and 60 µg/kg, and with or without SAC (10¹⁰ cells/kg) supplementation of 1 g/kg of dried yeast. Results showed that both AF and SAC increased average daily feed intake (both, p < 0.001) and reduced feed efficiency (p < 0.001 and p = 0.035, respectively), while only AF reduced average daily gain (p = 0.009). Supplementation with SAC improved the average daily feed intake in chickens subjected to AF60 (interaction, p < 0.001), suggesting that SAC could improve the appetite of broilers. Chickens fed AF had a lower carcass weight (p = 0.028) and heart weight (p = 0.031), but higher carcass-normalized weight of gizzard (p = 0.038) and liver (p = 0.010). Aflatoxin administration reduced white blood cells (p = 0.030), lymphocytes (p = 0.029) and basophils (p < 0.001), while increasing neutrophils (p = 0.009). SAC reduced neutrophils (p = 0.004) and mean corpuscular haemoglobin (p < 0.001) while increasing lymphocytes (p = 0.003) and basophils (p = 0.015). The haematological results suggest that AF caused a disturbance in the immune system, compromising the health of the chicken, whereas SAC potentially mitigates these alterations. Dietary AF increased the activity of glutamate oxaloacetate transaminase (p = 0.009). These findings suggest a potential use of Saccharomyces cerevisiae as a natural binder to reduce aflatoxicosis in poultry production systems. Full article