Search Results (360)

With the recent 5G communication technology deployment, Cellular Vehicle-to-Everything (C-V2X) significantly enhances road safety by enabling real-time exchange of critical traffic information among vehicles, pedestrians, infrastructure, and networks. However, further research is required to address real-time application latency and communication reliability challenges. This paper explores integrating cutting-edge C-V2X technology with environmental perception systems to enhance safety at intersections and crosswalks. We propose a multi-module architecture combining C-V2X with state-of-the-art perception technologies, GPS mapping methods, and the client–server module to develop a co-operative perception system for collision avoidance. The proposed system includes the following: (1) a hardware setup for C-V2X communication; (2) an advanced object detection module leveraging Deep Neural Networks (DNNs); (3) a client–server-based co-operative object detection framework to overcome computational limitations of edge computing devices; and (4) a module for mapping GPS coordinates of detected objects, enabling accurate and actionable GPS data for collision avoidance—even for detected objects not equipped with C-V2X devices. The proposed system was evaluated through real-time experiments at the GMMRC testing track at Kettering University. Results demonstrate that the proposed system enhances safety by broadcasting critical obstacle information with an average latency of 9.24 milliseconds, allowing for rapid situational awareness. Furthermore, the proposed system accurately provides GPS coordinates for detected obstacles, which is essential for effective collision avoidance. The technology integration in the proposed system offers high data rates, low latency, and reliable communication, which are key features that make it highly suitable for C-V2X-based applications. Full article

To foster the performance of wireless communication while saving energy, the integration of Intelligent Reflecting Surfaces (IRS) into autonomous vehicle (AV) communication networks is considered a powerful technique. This paper proposes a novel IRS-assisted vehicular communication model that combines Lagrange optimization and Gradient-Based Phase Optimization to determine the optimal transmission power, optimal interference transmission power, and IRS phase shifts. Additionally, the proposed model help increase the Signal-to-Interference-plus-Noise Ratio (SINR) by utilizing IRS, which leads to maximizes energy efficiency and the achievable data rate under a variety of environmental conditions, while guaranteeing that resource limits are satisfied. In order to represent dense vehicular environments, practical constraints for the system model, such as IRS reflection efficiency and interference, have been incorporated from multiple sources, namely, Device-to-Device (D2D), Vehicle-to-Vehicle (V2V), Vehicle-to-Base Station (V2B), and Cellular User Equipment (CUE). A Lagrangian optimization approach has been implemented to determine the required transmission interference power and the best IRS phase designs in order to enhance the system performance. Consequently, a one-dimensional convolutional neural network has been implemented for the optimized data provided by this framework as training input. This deep learning algorithm learns to predict the required optimal IRS settings quickly, allowing for real-time adaptation in dynamic wireless environments. The obtained results from the simulation show that the combined optimization and prediction strategy considerably enhances the system reliability and energy efficiency over baseline techniques. This study lays a solid foundation for implementing IRS-assisted AV networks in real-world settings, hence facilitating the development of next-generation vehicular communication systems that are both performance-driven and energy-efficient. Full article

Against the backdrop of intensified governance of territorial spatial planning, investigating carbon emissions from the perspective of territorial spatial planning for transport-land use integration holds significant academic and practical value. Taking Cangnan County as the case study, this research first dissects the reciprocal feedback mechanism between regional transport and land use at the territorial spatial planning level, while exploring transport-influencing factors. Subsequently, it constructs an integrated reciprocal feedback system for regional transport and land use by integrating accessibility drivers, cost matrices, and neighborhood weights through land use simulation–prediction models and the four-stage transport model. Finally, based on critical land use factors, diverse development scenarios under this integrated system are formulated; carbon emissions from transport and land use under each scenario are quantified; and their interrelationships are analyzed across multiple dimensions to explore the nexus of carbon emissions in transport–land use integration. Results indicate the following: (1) Integrated feedback enhances model accuracy (Kappa: 0.795→0.893; overall accuracy: 0.893→0.915), facilitating more precise land use simulation. (2) The county’s core construction area demonstrates the highest carbon emissions across all scenarios, meriting prioritized attention. (3) As deduced from the analysis of territorial spatial land use patterns, the significantly higher transport carbon emissions under the ecological protection priority scenario, compared to other scenarios, originate from over-concentrated construction land and imbalanced planning of carbon source land. These findings offer insights for regional planning; policy recommendations for Cangnan County include expanding carbon sink land, scientifically planning carbon source land, optimizing transport structures, and promoting new energy vehicles to advance carbon emission reduction and sustainable development. Full article

The phosphoinositide 3-kinase (PI3K)/AKT signaling pathway is a crucial regulator of cellular metabolism, proliferation, and survival. It is frequently dysregulated in metabolic, cardiovascular, and neoplastic disorders. Despite the advancements in multi-omics technology, existing methods often fail to provide real-time, pathway-specific insights for precision medicine and drug repurposing. We offer Agentic RAG-Driven Multi-Omics Analysis (ARMOA), an autonomous, hypothesis-driven system that integrates retrieval-augmented generation (RAG), large language models (LLMs), and agentic AI to thoroughly analyze genomic, transcriptomic, proteomic, and metabolomic data. Through the use of graph neural networks (GNNs) to model complex interactions within the PI3K/AKT pathway, ARMOA enables the discovery of novel biomarkers, probable candidates for drug repurposing, and customized therapy responses to address the complexities of PI3K/AKT dysregulation in disease states. ARMOA dynamically gathers and synthesizes knowledge from multiple sources, including KEGG, TCGA, and DrugBank, to guarantee context-aware insights. Through adaptive reasoning, it gradually enhances predictions, achieving 91% accuracy in external testing and 92% accuracy in cross-validation. Case studies in breast cancer and type 2 diabetes demonstrate that ARMOA can identify synergistic drug combinations with high clinical relevance and predict therapeutic outcomes specific to each patient. The framework’s interpretability and scalability are greatly enhanced by its use of multi-omics data fusion and real-time hypothesis creation. ARMOA provides a cutting-edge example for precision medicine by integrating multi-omics data, clinical judgment, and AI agents. Its ability to provide valuable insights on its own makes it a powerful tool for advancing biomedical research and treatment development. Full article

Background/Objectives: Radiotherapy can cause severe and irreversible brain damage, including cognitive impairment, increased dementia risk, debilitating depression, and other neuropsychiatric disorders. Current radioprotective drugs face limitations, such as single-target inefficacy or manufacturing hurdles. Isoliquiritigenin (ISL), a natural flavonoid derived from licorice root, exhibits broad bioactivities. It exhibits anti-inflammatory, anti-cancer, immunoregulatory, hepatoprotective, and cardioprotective activities. This study aimed to elucidate ISL’s neuronal radiation mitigation effects and key targets. Methods: In vitro and in vivo models of radiation-induced neuronal injury were established. ISL’s bioactivities were evaluated through cellular cytotoxicity assays, LDH release, ROS, ATP, glutamate, and GSH levels. In vivo, ISL’s radiation mitigation effect was evaluated with sucrose preference test, IL-β level, histopathological analysis, and Golgi-Cox staining analysis. Proteomics, pathway enrichment, and ensemble models (four machine learning models, weighted gene co-expression network, protein–protein interaction) identified core targets. Molecular docking and dynamic simulations validated ISL’s binding stability with key targets. Results: ISL attenuated radiation-induced cellular cytotoxicity, reduced LDH/ROS, restored ATP, elevated GSH, and mitigated glutamate accumulation. In rats, ISL alleviated anhedonia-like phenotypes and hippocampal synaptic loss. ISL also significantly suppressed radiation-induced neuroinflammation, as evidenced by reduced levels of the pro-inflammatory cytokine IL-1β. Proteomic analysis revealed that ISL’s main protective pathways included the synaptic vesicle cycle, glutamatergic synapse, MAPK signaling pathway, SNARE interactions in vesicular transport, insulin signaling pathway, and insulin secretion. Grm8, Grik3, and Grin3a were identified as key targets using the integrated models. The expression of these targets was upregulated post-radiation and restored by ISL. Molecular docking and dynamic simulations indicated that ISL showed stable binding to these receptors compared to native ligands. Conclusions: ISL demonstrates multi-scale radiation mitigation activities in vitro and in vivo by modulating synaptic and inflammatory pathways, with glutamate receptors as core targets. This work nominates ISL as an important natural product for mitigating radiotherapy-induced neural damage. Full article

Transcriptomic profiling is a powerful tool for dissecting the cellular and molecular complexity of ocular tissues, providing insights into retinal development, corneal disease, macular degeneration, and glaucoma. With the expansion of microarray, bulk RNA sequencing (RNA-seq), and single-cell RNA-seq technologies, artificial intelligence (AI) has emerged as a key strategy for analyzing high-dimensional gene expression data. This review synthesizes AI-enabled transcriptomic studies in ophthalmology from 2019 to 2025, highlighting how supervised and unsupervised machine learning (ML) methods have advanced biomarker discovery, cell type classification, and eye development and ocular disease modeling. Here, we discuss unsupervised techniques, such as principal component analysis (PCA), t-distributed stochastic neighbor embedding (t-SNE), uniform manifold approximation and projection (UMAP), and weighted gene co-expression network analysis (WGCNA), now the standard in single-cell workflows. Supervised approaches are also discussed, including the least absolute shrinkage and selection operator (LASSO), support vector machines (SVMs), and random forests (RFs), and their utility in identifying diagnostic and prognostic markers in age-related macular degeneration (AMD), diabetic retinopathy (DR), glaucoma, keratoconus, thyroid eye disease, and posterior capsule opacification (PCO), as well as deep learning frameworks, such as variational autoencoders and neural networks that support multi-omics integration. Despite challenges in interpretability and standardization, explainable AI and multimodal approaches offer promising avenues for advancing precision ophthalmology. Full article

In 2024, significant increases in surface temperature were recorded in Batam City and Bintan Regency, marking the highest levels observed in regional climate monitoring. The rapid conversion of vegetated land into residential and industrial areas has been identified as a major contributor to the acceleration of local climate warming. Climatological analysis also revealed extreme temperature fluctuations, underscoring the urgent need to understand spatial patterns of temperature distribution in response to climate change and weather variability. This research uses a Cellular Automata–Artificial Neural Network (CA−ANN) approach to model spatial and temporal changes in land surface temperature across the Riau Islands. To overcome the limitations of single-model predictions in a geographically diverse and unevenly developed region, Landsat satellite imagery from 2014, 2019, and 2024 was analyzed. Surface temperature data were extracted using the Brightness Temperature Transformation method. The CA−ANN model, implemented via the MOLUSCE platform in QGIS, incorporated additional environmental variables, such as rainfall distribution, vegetation density, and drought indices, to simulate future climate scenarios. Model validation yielded a Kappa accuracy coefficient of 0.72 for the 2029 projection, demonstrating reliable performance in capturing complex climate–environment interactions. The projection results indicate a continued upward trend in surface temperatures, emphasizing the urgent need for effective mitigation strategies. The findings highlight the essential role of remote sensing and spatial modeling in climate monitoring and policy formulation, especially for small island regions susceptible to microclimatic changes. Despite the strengths of the CA−ANN modeling framework, several inherent limitations constrain its application, particularly in the complex and heterogeneous context of tropical island environments. Notably, the accuracy of model predictions can be limited by the spatial resolution of satellite imagery and the quality of auxiliary environmental data, which may not fully capture fine-scale microclimatic variations. Full article

We investigate binary sequences generated by non-Markovian rules with memory length $\mu$, similar to those adopted in elementary cellular automata. This generation procedure is equivalent to a shift register, and certain rules produce sequences with maximal periods, known as de Bruijn sequences. We introduce a novel methodology for generating de Bruijn sequences that combines (i) a set of derived properties that significantly reduce the space of feasible generating rules and (ii) a neural-network-based classifier that identifies which rules produce de Bruijn sequences. The experiments for some values of $\mu$ demonstrate the approach’s effectiveness and computational efficiency. Full article

We compare several methods for predicting future built-up land cover using only a short yearly time series of satellite-derived binary urban maps. Existing methods of built-up expansion forecasting often rely on ancillary datasets such as utility networks, distance to transportation nodes, and population density maps, along with remotely sensed aerial or satellite imagery. Such ancillary datasets are not always available and lack the temporal density of satellite imagery. Moreover, existing work often focuses on quantifying the expected volume of built-up expansion, rather than predicting where exactly that expansion will occur. To address these gaps, we evaluate six methods for the creation of prediction maps showing expected areas of future built-up expansion, using yearly built/not-built maps derived from Sentinel-2 imagery as inputs: Cellular Automata, logistic regression, Support Vector Machines, Random Forests, Convolutional Neural Networks (CNNs), and CNNs with the addition of long short-term memory (ConvLSTM). Of these six, we find CNNs to be the best-performing method, with an average Cohen’s kappa score of 0.73 across nine study sites in the continental United States. Full article

Understanding the drivers of water yield (WY) changes in ecologically sensitive, data-scarce watersheds is crucial for sustainable management, particularly in the context of accelerating forest expansion and urbanization. This study focuses on the upper Yellow River Basin (UYRB), a critical headwater region that supplies 60% of the Yellow River’s flow and is undergoing rapid land use transitions from 1990 to 2100. Using the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model and the Future Land-Use Simulation (FLUS) model, we quantify historical (1990–2020) and projected (2025–2100) WY dynamics under three SSP scenarios (SSP126, SSP370, and SSP585). InVEST, a spatially explicit ecohydrological model based on the Budyko framework, estimates WY by balancing precipitation and evapotranspiration. The FLUS model combines cellular automata (CA) with an artificial neural network (ANN)-based suitability evaluation and Markov chain-derived transition probabilities to simulate land-use change under multiple scenarios. Results show that WY increased significantly during the historical period (1990–2020), primarily driven by increased precipitation, with climate change accounting for 94% and land-use change for 6% of the total variation in WY. Under future scenarios (SSP126, SSP370, and SSP585), WY is projected to increase to 217 mm, 206 mm, and 201 mm, respectively. Meanwhile, the influence of land-use change is expected to diminish, with its contribution decreasing to 9.1%, 5.7%, and 3.1% under SSP126, SSP370, and SSP585, respectively. This decrease reflects the increasing strength of climate signals (especially extreme precipitation and evaporative demand), which masks the hydrological impacts of land-use transitions. These findings highlight the dominant role of climate change, the scenario-dependent effects of land-use change, and the urgent need for integrated climate–land management strategies in forest-urbanizing watersheds. Full article

As typical environmental hormones, endocrine-disrupting chemicals (EDCs) have become a global environmental health issue of high concern due to their property of interfering with the endocrine systems of organisms. As a commonly used substitute for bisphenol A (BPA), bisphenol E (BPE) has been frequently detected in environmental matrices such as soil and water in recent years. Existing research has unveiled the developmental and reproductive toxicity of BPE; however, only one in vitro cellular experiment has preliminarily indicated potential neurotoxic risks, with its underlying mechanisms remaining largely unelucidated in the current literature. Potential toxic mechanisms and action targets of BPE were predicted using the zebrafish model via network toxicology and molecular docking, with RT-qPCRs being simultaneously applied to uncover neurotoxic effects and associated mechanisms of BPE. A significant decrease (p < 0.05) in the frequency of embryonic spontaneous movements was observed in zebrafish at exposure concentrations ≥ 0.01 mg/L. At 72 hpf and 144 hpf, the larval body length began to shorten significantly from 0.1 mg/L to 1 mg/L, respectively (p < 0.01), accompanied by a reduced neuronal fluorescence intensity and a shortened neural axon length (p < 0.01). By 144 hpf, the motor behavior in zebrafish larvae was inhibited. Through network toxicology and molecular docking, HSP90AB1 was identified as the core target, with the cGMP/PKG signaling pathway determined to be the primary route through which BPE induces neurotoxicity in zebrafish larvae. BPE induces neuronal apoptosis and disrupts neurodevelopment by inhibiting the cGMP/PKG signaling pathway, ultimately suppressing the larval motor behavior. To further validate the experimental outcomes, we measured the expression levels of genes associated with neurodevelopment (elavl3, mbp, gap43, syn2a), serotonergic synaptic signaling (5-ht1ar, 5-ht2ar), the cGMP/PKG pathway (nos3), and apoptosis (caspase-3, caspase-9). These results offer crucial theoretical underpinnings for evaluating the ecological risks of BPE and developing environmental management plans, as well as crucial evidence for a thorough comprehension of the toxic effects and mechanisms of BPE on neurodevelopment in zebrafish larvae. Full article

Glioblastoma (GBM) remains one of the most aggressive and treatment-resistant brain tumors, largely due to its profound intratumoral heterogeneity and immunosuppressive microenvironment. Various classifications of GBM subtypes were created based on transcriptional and methylation profiles. This effort, followed by the development of new technology such as single-nuclei sequencing (snRNAseq) and spatial transcriptomics, led to a better understanding of the glioma cells’ plasticity and their ability to transition between diverse cellular states. GBM cells can mimic neurodevelopmental programs to resemble oligodendrocyte or neural progenitor behavior and hitchhike the local neuronal network to support their growth. The tumor microenvironment, especially under hypoxic conditions, drives the tumor cell clonal selection, which then reshapes the immune cells’ functions. These adaptations contribute to immune evasion by progressively disabling T cell and myeloid cell functions, ultimately establishing a highly immunosuppressive tumor milieu. This complex and metabolically constrained environment poses a major barrier to effective antitumor immunity and limits the success of conventional therapies. Understanding the dynamic interactions between glioma cells and their microenvironment is essential for the development of more effective immunotherapies and rational combination strategies aimed at overcoming resistance and improving patient outcomes. Full article

Hydraulic shear has been widely accepted as one of the essential factors modulating phytoplankton growth. Previous experimental studies of algal growth have been conducted at the macroscopic level, and direct observation at the cell scale has been lacking. In this study, an algal-cell dynamic continuous observation platform (ACDCOP) is proposed with a parallel-plate flow chamber (PPFC) to capture cellular growth images which are then used as input to a computer vision algorithm featuring a pre-trained backpropagation neural network to quantitatively evaluate the volumes and volumetric growth rates of individual cells. The platform was applied to investigate the growth of Scenedesmus quadricauda cells under different hydraulic shear stress conditions. The results indicated that the threshold shear stress for the development of Scenedesmus quadricauda cells was 270 µL min⁻¹ (5.62 × 10⁻⁵ m² s⁻³). Cellular growth was inhibited at very low and very high intensities of hydraulic shear. Among all the experimental groups, the longest growth period for a cell, from attachment to PPFC to cell division, was 5.7 days. Cells with larger initial volumes produced larger volumes at division. The proposed platform could provide a novel approach for algal research by enabling direct observation of algal growth at the cell scale, and could potentially be applied to investigate the impacts of various environmental stressors such as nutrient, temperature, and light on cellular growth in different algal species. Full article

Background: Urine microbial analysis is a frequently requested test that is often associated with contamination during specimen collection or storage, which leads to false-positive diagnoses and delayed reporting. In the era of digitalization, machine learning (ML) can serve as a valuable tool to support clinical decision-making. Methods: This study investigates the application of a simple artificial neural network (ANN) to pre-identify negative and contaminated (false-positive) specimens. An ML model was developed using 8181 urine samples, including cytology, dipstick tests, and culture results. The dataset was randomly split 2:1 for training and testing a multilayer perceptron (MLP). Input variables with a normalized importance below 0.2 were excluded. Results: The final model used only microbial and either urine color or urobilinogen pigment analysis as inputs; other physical, chemical, and cellular parameters were omitted. The frequency of positive and negative specimens for bacteria was 6.9% and 89.6%, respectively. Contaminated specimens represented 3.5% of cases and were predominantly misclassified as negative by the MLP. Thus, the negative predictive value (NPV) was 96.5% and the positive predictive value (PPV) was 87.2%, leading to 0.82% of the cultures being unnecessary microbial cultures (UMC). Conclusions: These results suggest that the MLP is reliable for screening out negative specimens but less effective at identifying positive ones. In conclusion, ANN models can effectively support the screening of negative urine samples, detect clinically significant bacteriuria, and potentially reduce unnecessary cultures. Incorporating morphological information data could further improve the accuracy of our model and minimize false negatives. Full article

Investigating land cover patterns, changes in carbon stocks, and forecasting future conditions are essential for formulating regional sustainable development strategies and enhancing ecological and environmental quality. This study centers on Guiyang, a mountainous urban area in southwestern China, to analyze the dynamic changes in land cover and their effects on carbon stocks from 2000 to 2035. A carbon stocks assessment framework was developed using a cellular automaton-based artificial neural network model (CA-ANN), the InVEST model, and the geographical detector model to predict future land cover changes and identify the primary drivers of variations in carbon stocks. The results indicate that (1) from 2000 to 2020, impervious surfaces expanded significantly, increasing by 199.73 km². Compared to 2020, impervious surfaces are projected to increase by 1.06 km², 13.54 km², and 34.97 km² in 2025, 2030, and 2035, respectively, leading to further reductions in grassland and forest areas. (2) Over time, carbon stocks in Guiyang exhibited a general decreasing trend; spatially, carbon stocks were higher in the western and northern regions and lower in the central and southern regions. (3) The level of greenness, measured by the normalized vegetation index (NDVI), significantly influenced the spatial variation of carbon stocks in Guiyang. Changes in carbon stocks resulted from the combined effects of multiple factors, with the annual average temperature and NDVI being the most influential. These findings provide a scientific basis for advancing low-carbon development and constructing an ecological civilization in Guiyang. Full article