Search Results (2,787)

(1) Background: There has been an increase in the utilization of the minimally invasive vertical right axillary thoracotomy approach for repairing congenital heart defects in children recently. We aim, in the current study, to evaluate the outcomes of this approach in repairing anomalous pulmonary venous connections with or without an associated sinus venosus defect. (2) Methods: A total of 23 consecutive patients underwent surgical repair of anomalous pulmonary venous connections between April 2018 and February 2024. Perioperative and clinical follow-up data were obtained. (3) Results: The median age and weight were 36 months (1–277 months) and 14.4 kg (3.6–79.4 kg), respectively. More than half were females (13; 56.5%). There was no conversion to sternotomy. Partial anomalous pulmonary venous connections were the most frequent primary diagnoses (14; 60.9%), followed by scimitar syndrome (3; 13%), while two patients (8.7%) had total anomalous pulmonary venous connections. Repair techniques included single patch in 10 patients (43.5%), Warden in 6 (26.1%), and two-patch technique in 4 (17.4%). The median cardiopulmonary bypass and aortic cross-clamp times were 91 and 62 min, respectively. All patients were extubated in the operating room. The median length of hospital stay was 2 days. There were no mortalities or reoperations for pulmonary/systemic venous pathway obstruction. (4) Conclusions: Vertical right axillary thoracotomy is a valuable approach for repairing anomalous pulmonary venous connections with or without sinus venosus defects. All repair techniques, including Warden and scimitar, can be performed safely through this approach. The cosmetic superiority and short hospital stay make this approach worth considering. Full article

Additive manufacturing offers significant design freedom for injection mold tooling, particularly in optimizing cooling performance and reducing mass. This study presents a holistic framework for the topology optimization of mold inserts considering design for additive manufacturing principles, integrating essential boundary conditions from the mold making, injection molding process, and post-processing operations. A slider component with conformal cooling channels serves as the case study. Using simulation-driven design and finite element analysis, two design variants, based on conventional and modified design spaces, were evaluated. Mechanical loads from clamping and the injection process were considered, with safety factors applied to reflect industrial misuse scenarios. The topology optimization process was implemented using Altair OptiStruct and validated through displacement and stress analyses. The results show savings in both mass and costs of up to 60% while maintaining structural integrity under operational and misuse conditions. The maximum displacements—only a 4 µm increase compared to the reference—remained within DIN ISO 20457 tolerances, and stresses did not exceed 170 MPa under operational conditions, confirming industrial applicability. This study concludes with a proposed framework for integrating topology optimization into mold design workflows. Full article

Steel structures that support machines and industrial process installations should ideally be flexible, adaptable, and easily reconfigurable. However, in current practice, new profiles are frequently used and discarded whenever layout modifications are required, leading to considerable material waste, increased costs, and environmental burdens. Such practices conflict with the principles of the circular economy, in which reusability is preferable to recycling. This paper presents a life cycle sustainability assessment (life cycle cost, LCC, and life cycle assessment, LCA) applied to six structural typologies: (a) welded IPE profiles, (b) bolted IPE profiles, (c) welded tubular profiles, (d) bolted tubular profiles, (e) clamped IPE profiles with demountable joints, and (f) flanged tubular profiles with demountable joints. The assessment integrates structural calculations with an updatable database of costs, operation times, and service lives, providing a systematic framework for evaluating both economic and environmental performance in medium-load industrial structures (0.5–9.8 kN/m²). Application to nine representative case studies demonstrated that demountable clamped and flanged joints become economically competitive after three life cycles, and after only two life cycles under high-load conditions (9.8 kN/m²). The findings indicate relative cost savings of up to 75% in optimized configurations and carbon-footprint reductions of approximately 50% after three cycles. These results provide quantitative evidence of the long-term advantages of demountable and reconfigurable steel structures. Their capacity for repeated reuse without loss of performance supports sustainable design strategies, reduces environmental impacts, and advances circular economy principles, making them an attractive option for modern industrial facilities subject to frequent modifications. Full article

A contactless passive magnetoelastic sensing setup, recently proposed for detecting pest/substance accumulation in confined spaces (labs, museum reserves), is optimized for enhanced low-frequency performance. The setup uses a short flexible polymer slab, clamped at one end. There, a short Metglas^® 2826MB magnetoelastic ribbon is fixed upon the slab’s surface. The opposite end receives excitation by a remotely controlled module of ultra-low amplitude vibration. When vibrating (with the slab), the ribbon generates magnetic flux, which depends on (and reflects) the slab’s dynamics. This changes when loads accumulate on its surface. The flux induces voltage in a contactless manner in a low-cost pick-up coil suspended above the ribbon. Voltage monitoring allows for evaluation of the vibrating slab’s real-time dynamics and, consequently, the detection of load-induced changes. This work innovates by introducing a low-cost passive circuit for real-time voltage processing, thus achieving an accurate representation of the low-frequency dynamics of the magnetic flux. Furthermore, it introduces an algorithm, which statistically detects load-induced changes using the voltage’s low-frequency power characteristics. Both additions enable load detection at relatively low frequencies, thus addressing a principal issue of passive contactless sensing setups. Extensive testing at different occasions demonstrates promising load detection performance under various conditions, especially given its cost-efficient hardware and operation. Full article

Offshore platforms need to be made, from the start of their construction, to withstand the extreme environmental conditions they will be facing. This study investigates the welding-induced residual stress and distortion in a Y-shaped tubular joint extracted from an offshore wind turbine jacket substructure. While similar joints are commonly used in offshore platforms, their welding behavior remains underexplored in the existing literature. The joint configuration is representative of critical load-bearing connections commonly used in offshore platforms exposed to harsh marine environments. A finite element model has been developed to simulate the welding process in a typical offshore tubular joint through thermal and mechanical simulation. Validation of the model has been achieved with results against reference experimental data, with temperature and distortion errors of 3.9 and 5.3%, respectively. Residual stress and distortions were analyzed along predefined paths in vertical, transverse, and longitudinal directions. A mesh sensitivity study was conducted to balance computational efficiency and result accuracy. Furthermore, clamped and free displacement boundary conditions are analyzed, demonstrating reduced deformation and stress for the second case. Full article

Background: Robot-assisted partial nephrectomy (RAPN) is a standard approach for localized renal cell carcinoma (RCC), emphasizing renal functional preservation. The Minimum Layer Resection (MLR) method, guided by 3D virtual partial nephrectomy (3DvPN) planning, was developed to balance oncological safety with parenchymal preservation. This study evaluated functional and oncological outcomes of RAPN with MLR and identified predictors of renal functional decline. Methods: We retrospectively analyzed 237 patients (after screening 312 cases) who underwent RAPN between 2012 and 2022 with ≥36-month follow-up. 3DvPN planning was used to guide MLR when feasible; both MLR and non-MLR were available and applied throughout the study period according to predefined indications. The primary endpoint was the percentage of estimated glomerular filtration rate (eGFR) preservation at 36 months; a ≥10% decline was clinically significant. Secondary endpoints included perioperative outcomes, acute kidney injury (AKI), and oncological outcomes such as margin involvement and recurrence. Results: The median age was 60 years, tumor size 29 mm, and warm ischemia time 21 min, with selective or superselective clamping achieved in 62.8% of cases. Postoperative AKI occurred in 25.0% (no patient required dialysis). At 3 years, the median eGFR preservation rate was 84.4%, and 28.5% of patients experienced a ≥10% decline. Independent predictors of short-term decline (14 days) were BMI > 25 kg/m², AKI, and WIT > 25 min, whereas long-term decline (36 months) was associated with tumor size > 30 mm and WIT > 25 min. Margin involvement was 1.7%, recurrence 3.8%, and major complications (Clavien–Dindo ≥IV) occurred in 1.7%. Conclusions: In conclusion, RAPN with the MLR technique under 3DvPN guidance demonstrated favorable perioperative outcomes, acceptable oncologic safety, and good mid-term renal functional preservation (up to 36 months). The approach provides a reproducible surgical strategy that maximizes parenchymal preservation while maintaining negative surgical margins. Prospective multicenter studies with longer follow-up are warranted to confirm long-term durability and to define the role of MLR in routine practice. Full article

Understanding the functional modulation of ion channels by multiple activating substances is critical to grasping stimulus-specific gating mechanisms and possible synergistic or competitive interactions. This study investigates the activation of large-conductance, voltage- and Ca²⁺-activated potassium channels (BK) in the plasma membrane of human bronchial epithelial cells by Ca²⁺ and quercetin (Que), both individually and in combination. Patch-clamp recordings were analyzed using open state probability, dwell-time distributions, Shannon entropy, sample entropy, power spectral density (PSD), and empirical mode decomposition (EMD). Our results reveal concentration-dependent alterations in gating kinetics, particularly at a low concentration of quercetin ([Que] = 10 μM) compared with [Que] = 100 μM, where some Que-related effects are strongly attenuated in the presence of Ca²⁺. We also identify specific frequency bands where oscillatory components are most sensitive to the considered stimuli. Our findings highlight the complex reciprocal interplay between Ca²⁺ and Que in modulating BK channel function, and demonstrate the interpretative power of entropic and signal-decomposition approaches in characterizing stimulus-specific gating dynamics. Full article

In winter, stay cable sheaths are prone to icing, which increases cable loads and poses a falling-ice hazard upon thawing. While manual and chemical de-icing are common methods, their safety and cost drawbacks make robotic de-icing a promising alternative. Robotic de-icing offers a promising alternative. However, to protect the sheath from damage, the de-icing blade is designed to minimize contact with its surface. Consequently, a thin layer of residual ice is often left behind, which reduces the surface friction coefficient and complicates the climbing process. This study evaluates the climbing performance of a self-manufactured cable-climbing mechanism through laboratory tests and dynamic simulations (ADAMS). A physical prototype was built, and dynamic simulations of the cable-climbing mechanism were conducted using Automated Dynamic Analysis of Mechanical Systems (ADAMS) software. The preliminary validation results demonstrate that the mechanism is capable of maintaining stable climbing under extreme conditions, including a friction coefficient of 0.12 to reflect thin-ice variability and indicated stable climbing even at μ = 0.12), a vertical inclination of 90°, and a load of 12 kg, confirming the design’s validity. Furthermore, we analyzed key parameters. A lower friction coefficient requires a higher clamping force and adversely affects the climbing speed due to increased slip. Similarly, an increased payload elevates the mechanism’s deflection angle, spring force, and wheel torque, which in turn reduces the climbing speed. Cable inclination has a complex effect: deflection decreases with slope, yet clamping force peaks near 70°, showing a bell-shaped trend. This peak requirement dictated the damping spring selection, which was given a safety margin. This ensures safe operation and acceleration at all other angles. Limitations: The present results constitute a feasibility validation under controlled laboratory conditions and rigid-support simulations. The long-term effects of residual ice and field performance remain to be confirmed in planned field trials. Full article

We propose a GPU-parallelized collision-detection and response framework for rigid-body dynamics, designed to efficiently handle densely populated 3D simulations in real time. The method combines explicit Euler time integration with a hierarchical Octree–AABB collision-detection scheme, enabling early pruning and localized refinement of contact checks. To resolve collisions, we employ a two-step response algorithm that integrates non-penetration correction and impulse-based velocity updates, stabilized through smoothing, clamping, and bias mechanisms. The framework is fully implemented within Unity3D using compute shaders and optimized GPU kernels. Experiments across multiple mesh models and increasing object counts demonstrate that the proposed hierarchical configuration significantly improves scalability and frame stability compared to conventional flat AABB methods. In particular, a two-level hierarchy achieves the best trade-off between spatial resolution and computational cost, maintaining interactive frame rates (≥30 fps) under high-density scenarios. These results suggest the practical applicability of our method to real-time simulation systems involving complex collision dynamics. Full article

The Anti-roll Bar is a critical component of the automobile suspension system. The stiffness of the Anti-roll Bar significantly impacts the suspension stiffness and is related to the interference of the rubber bushing. To obtain reasonable Anti-roll Bar stiffness and determine the appropriate amount of rubber bushing interference, a certain type of automotive lateral Anti-roll Bar model was established through ANSYS Workbench finite element analysis for the rubber bushing material using a Mooney–Rivlin two-parameter model simulation. A different amount of interference was set up between the rubber bushing and the various parts of the bushing. The overload simulation was performed to simulate the bushing in an overload state with a different amount of interference between the rubber bushing and the various parts of the bushing. The stresses of the three main parts of the Anti-roll Bar (the clamp, skeleton, and bushing) were analyzed in an overload state. The radial and torsional stiffness values of the Anti-roll Bar are analyzed under the interference state. The influence of interference fit variations in different mating parts on the radial and torsional stiffness of the Anti-roll Bar is studied. It is determined that the stiffness value of the Anti-roll Bar meets the requirements when the interference fit between the bushing and rod is 1 mm, the bushing-plate interference is 2 mm, and the interference fit between the bushing and clamp is 0.6 mm, provided that the strength requirements of each part are met. This study provides important reference significance for designing. Full article

Mutation T226R in the Kv1.1 α-subunit of voltage-gated potassium Kv1 channels is associated with episodic ataxia type 1, severe neuromyotonia, and epilepsy. In vitro, this mutation was reported to considerably distort the functioning of homotetrameric channels Kv1.1; however, in the brain, Kv1.1 α-subunits form heterochannels predominantly associating with Kv1.2 α-subunits. Using the patch-clamp technique, fluorescent and Förster resonance energy transfer confocal microscopy, we revealed that heterochannels Kv(1.1(T226R)-1.2)₂ formed by concatemers Kv1.1(T226R)-Kv1.2 in Neuro-2a cells have significantly slower activation and deactivation rates, and their activation occurs at a much less negative membrane potential compared to channels Kv(1.1-1.2)₂ formed by concatemers Kv1.1-Kv1.2. This mutation does not noticeably affect the formation of complexes between α-subunits Kv1.1 and Kv1.2, but it does induce a delayed and possibly decreased presentation of heterochannels Kv(1.1(T226R)-1.2)₂ on the plasma membrane. At the same time, the T226R mutation has a much stronger negative effect on the membrane presentation of homotetrameric Kv1.1 channels. Since heterochannels Kv1.1-Kv1.2 but not homotetrameric channels Kv1.1 are present in the brain, the heterochannels bearing mutation T226R are most likely underlying the pathogenesis of the disease by decreasing the responsiveness of cells to mild membrane depolarization and, thus, increasing the excitability of neurons. Full article

Two-pore channel 2 (TPC2) is a member of the endolysosomal ion channel family, playing critical roles in intracellular calcium signaling and endomembrane dynamics. This review provides an in-depth analysis of TPC2, covering its structural and functional properties, physiological roles, and involvement in human diseases. We discuss current experimental approaches to studying TPC2, including heterologous expression in plant vacuoles and computational modeling strategies. Particular emphasis is placed on the structural determinants of ion permeation, with a focus on the selectivity filter and the central cavity’s influence on channel kinetics. Furthermore, we explore emerging roles of TPC2 in viral infections, particularly SARS-CoV-2, and in cancer, including melanoma progression and neoangiogenesis. The inhibitory potential of natural compounds, such as naringenin, is also examined. By offering a comprehensive overview of current knowledge and methodologies, this review underscores the potential of TPC2 as a promising pharmacological target in both infectious and neoplastic diseases. Full article

Introduction: In minimally invasive, off-clamp partial nephrectomy (ocMIPN), the reconstructive strategy profoundly influences functional outcomes. Traditional nephrometry scores aid preoperative planning but do not directly inform the choice of closure technique. This dual-institutional study aimed primarily to identify preoperative CT-derived parameters predictive of renorrhaphy versus a sutureless approach, and secondarily to compare perioperative and functional outcomes between these techniques. Methods: We retrospectively analyzed 201 consecutive ocMIPN cases performed using a standardized off-clamp technique by two experienced surgical teams across robotic platforms and conventional laparoscopy. Preoperative CT scans were centrally reviewed to quantify morphometric features, including contact surface area (CSA), tumor radius, and Gerota’s fascia thickness. Univariable and multivariable logistic regression models—one restricted to radiologic variables and one expanded with RENAL score terms—were generated to identify independent predictors. Perioperative outcomes, renal functional metrics, and Trifecta rates were compared between cohorts. Results: Among the 201 patients, 101 (50.2%) underwent sutureless reconstruction and 100 (49.8%) renorrhaphy. Cohorts were comparable at baseline except for tumor size (3.1 vs. 3.6 cm; p = 0.04). In multivariable analysis, CSA > 15 cm² (OR 3.93; 95% CI 1.26–12.26; p = 0.02) and tumor radius (OR 1.14 per mm; 95% CI 1.01–1.29; p = 0.04) consistently predicted renorrhaphy, while Gerota’s fascia < 10 mm emerged as significant only in the expanded specification (OR 0.08; 95% CI 0.01–0.70; p = 0.02). Integration with RENAL improved predictive performance (ΔAUC 0.06; NRI 0.14; IDI 0.07), and the final model demonstrated strong discrimination (AUC 0.81) with satisfactory calibration. Perioperative outcomes, postoperative renal function, and Trifecta achievement were similar between groups (all p ≥ 0.21). Conclusions: A concise set of CT-derived morphologic markers—CSA, tumor radius, and perinephric fascia thickness—anticipated reconstructive strategy in ocMIPN and augmented the discriminatory power of RENAL nephrometry. When anatomy was favorable, sutureless repair was not associated with statistically significant differences in perioperative safety or renal function, although the study was not powered for formal equivalence testing. These findings support the integration of radiologic markers into preoperative planning frameworks for nephron-sparing surgery. Full article

An accelerometer mounting technique has large implications on the frequency range and accuracy of the measurement, with stiffness and the mass relative to the monitored structure as the primary concerns. The International Organization for Standardization (ISO) gives an extensive list in 5348:2021, detailing mounting methods, and provides recommendations for testing mounts that are not specifically defined. In the nuclear industry on the laboratory scale, there is a need for vibration measurements for predictive maintenance and process monitoring that are nondestructive and capable of working in high-temperature environments. Commercial adhesive products with easy application and removal were tested as nondestructive methods, while quick mounts to a commonly used aluminum frame were tested as nondestructive and have potential applicability in high-temperature environments. The sinusoidal excitation method was used, measuring frequencies from 50 Hz to 10 kHz in one-third octave band intervals, utilizing three accelerometers and comparing the results to those obtained with the stud-mounting method. Using the lowest ±3 dB threshold across each accelerometer, foam dots and poster strips were not successful, and foam tapes were accurate up to 2000 Hz, hose clamps and zip ties up to 800 Hz, and a custom 3D printed mount up to 1000 Hz. Knowing the limitations of each mounting technique allows for accurate measurements within the appropriate range. Full article

The hyperinsulinemic–euglycemic clamp technique is considered the gold standard for measuring insulin sensitivity in large animals. We developed a practical method for conducting concurrent glucose clamp experiments in a pair of sedated farm swine positioned in a sling. Descriptions of customized equipment and central venous access surgical procedures for blood collection are provided. Personnel functions are described for execution of the clamp protocol. A total of 24 hyperinsulinemic–euglycemic clamp studies were performed over 6 weeks. Infusaports remained functional for 1454 blood samples. There were three CSII catheter occlusions during bolus administration, and the swine showed no signs of infection or disease. IM telazol at 1.0 mg/kg, administered 1–2 h prior (mean of 3.26 mL ± 1.59) was effective in keeping animals comfortable. SpO₂ and heart rate remained within normal ranges. Means ± SD total infused volumes for octreotide, 10% dextrose, and saline were 9.7 ± 0.93 mL, 2328.0 ± 672.8 mL, and 690.3 ± 206.8 mL. Mean blood glucose was maintained between 75.7 and 87.8 mg/dL (CV 3.17%) for the 24 experiments. The GIR infusion rate peaked between 15 and 60 min after insulin bolusing, with insulin C_max of 108.5 pmol/L and t_max at 10 min. All aspects of the protocol were effectively carried out. The animals remained in good health, and the implanted infusion ports remained patent for over 700 blood draws per animal. This method could potentially reduce the number of animals used and the costs of other similar experiments. Full article