Search Results (73)

Background: Disease relapse is a primary cause of treatment failure after hematopoietic stem cell transplantation in the treatment of malignancy. Consolidation therapy early after transplantation may reduce this risk, but it is difficult to administer in the setting of various post-transplant complications. We proposed that testing donor cell chimerism and for persistent minimal residual disease (MRD) with next-generation sequencing (NGS) of plasma cell-free DNA (cfDNA) early after transplantation would identify those patients at higher risk of relapse who would possibly benefit from consolidation therapy. Methods: We enrolled 20 subjects with known tumor-associated somatic mutations into this prospective pilot study, testing plasma samples before and at 28, 56, and 84 days after transplantation. Pre- and post-transplant bone marrow samples were also analyzed. All samples were subjected to an agnostic, commercially available panel covering 302 genes. Results: Significantly more mutations (p < 0.0001) were detected in the plasma cfDNA than in the bone marrow cells in pre-transplant testing (92 versus 61 mutations, respectively), most likely reflecting sampling variation when bone marrow was used. Two subjects were negative for MRD in staging studies immediately before transplants. Most (19/20) subjects had intermittent or sustained MRD detected in post-transplant plasma cfDNA testing, albeit with much lower average variant allele frequencies (VAFs). Six out of 20 subjects suffered relapses within 12 months after transplantation, and all 6 could be identified by adverse-risk driver mutations that persisted after transplantation. No patients who cleared the adverse-risk mutations relapsed. Donor chimerism using cfDNA fell for all relapsed patients and contributed to the identification of patients at early risk for relapse. Conclusions: These data demonstrate that testing plasma cfDNA for persistent leukemia-associated somatic mutations and donor chimerism as early as 28 days after transplantation will identify a subset of patients with high-risk mutations who are at high risk of relapse. This early assessment of relapse risk may facilitate modifications to the treatment plan, reducing the risk of treatment failure. Full article

A coal seam from the Fushun Basin in NE China was investigated pertaining to its bulk and molecular compositions to elucidate its hydrocarbon generation potential. Eocene Guchengzi Formation coals and carbonaceous mudstones were deposited in oxic environments and fell within the subbituminous A rank. Hydrogen index (HI) values spanned within a range from 139 to 495 mg HC/g total organic carbon (TOC), indicating the presence of Type II2-III kerogens. The pyrolytic hydrocarbon yield (S₂) linearly increased with TOC content in carbonaceous mudstones, while there was no evident correlation between S₂ and TOC in the coals. Molecular compositions revealed that the input of algae and aquatic biomass did not enhance the hydrocarbon generation potential of the coals. Moreover, the hydrocarbon generation potential of the coals was not influenced by changes in flora. However, the degree of terpenoid aromatization showed a clear correlation with the HI values. A higher hydrocarbon generation potential is associated with a lower degree of aromatization, even though the Pristane/Phytane ratio does not indicate such a correlation. Bacterial transformation played a pivotal role in the structural rearrangement of the coal matrix, resulting in hydrogen incorporation into the coal. This ultimately led to a relatively hydrogen-rich composition with high oil-generating potential. Full article

Liver ischaemia–reperfusion (IR) injury remains a major cause of morbidity and mortality following liver transplantation and resection. CD4+ T cells have been shown to play a key role in murine models; however, there is currently a lack of data that support their role in human patients. Methods: Data on clinical outcomes and complications were documented prospectively in 28 patients undergoing first elective liver transplant surgery. Peripheral blood samples were collected at baseline (pre-op), 2 h post graft reperfusion, immediately post-op, and 24 h post-op. A post-reperfusion biopsy was analysed in all patients, and in five patients, a donor liver biopsy was available pre-implantation. Circulating cytokines were measured, and T cells were analysed for activation markers and cytokine production. Results: Circulating levels of cytokines associated with innate immune cell recruitment and activation were significantly elevated in the peri-transplant period. High circulating IL-10 levels corresponded with the development of graft-specific complications. The proportion of CD4+ T cells in the peripheral circulation fell throughout the peri-operative period, suggesting CD4+ T cell recruitment to the graft. Although TNFα was the predominant cytokine produced by CD4+ T cells in the intrahepatic environment, the production of IFNγ was significantly upregulated by circulating CD4+ T cells. Furthermore, we demonstrated clear recruitment of inflammatory monocytes in the peri-operative period. In donor-and-recipient pairs with a mismatch at the HLA-A2 or A3 allele, we demonstrated that inflammatory monocytes in the liver are recipient-derived. Discussion: This is the first study to our knowledge that tracks early immune cell responses in humans undergoing liver transplantation. The recruitment of inflammatory monocytes from the recipient and their cytokine release is associated with liver-specific complications. Inflammatory monocytes would be an attractive target to ameliorate ischaemia–reperfusion injury. Full article

To estimate the potential and realized carbon emission reductions from implementation of reduced-impact logging (RIL) in Indonesia, we compiled logging emissions data from 15 concessions in Kalimantan and 10 from the Papuan provinces. Committed emissions data were collected for harvested timber as well as from collateral damage caused by felling, skidding, and clearing for haul roads and log yards. Emissions expressed as mean ± standard error per cubic meter of timber harvested, per area harvested, and per Mg of timber harvested (i.e., the ‘Carbon Impact Factor’) were 1.30 ± 0.15 Mg C m⁻³, 27.52 ± 4.44 Mg C ha⁻¹, and 6.88 ± 0.84 Mg Mg⁻¹, respectively. Among the sampled concessions, felling, hauling, and skidding caused 18–86%, 2–48%, and 6–75% of these emissions, respectively. Potential emission reductions calculated as the difference between observed emissions and those of the five best-performing concessions are 0.67 ± 0.15 Mg C m⁻³, 21.11 ± 4.38 Mg C ha⁻¹, and 4.20 ± 0.83 Mg Mg⁻¹, which represents reductions of 51%, 76%, and 61%, respectively. Extrapolating these estimates to all of Indonesia using average log production data from 2018 to 2021 results in an estimated annual emissions reduction of 14.47 Tg CO₂ from full adoption of RIL, which is 2.9% of Indonesia’s nationally determined contribution (NDC) from the forestry sector. Full article

The large pine weevil (Hylobius abietis L.) is a major pest in European and Asian coniferous forests, particularly in managed plantations where clear-felling practices create ideal conditions for its population growth. Traditional management practices involving synthetic insecticides have limited efficacy in terms of reducing pest populations and pose environmental risks. This study evaluated the effectiveness of a wild entomopathogenic fungus (EPF) and the commercial entomopathogenic nematode Steinernema carpocapsae (EPN) as biological control agents (BCAs) against H. abietis in clear-felled spruce plantations in Wales and Scotland. Field trials used a randomised block design with three treatments (EPN full dose, EPF full dose and a combination of EPF+EPN at half doses each) compared to a control. Emergence trapping and destructive sampling were employed to assess treatment efficacy. All treatments significantly reduced weevil emergence, with the mixed treatment showing the greatest impact. Destructive sampling revealed strong associations between treatment type and infection outcomes in H. abietis, with a small but significant relationship between weevil developmental stages and infection types. Importantly, the treatments had no significant impact on the total abundance or taxon richness of non-target invertebrates. These findings suggest that wild EPFs alone and combined with EPNs are effective and environmentally safe alternatives to synthetic insecticides for managing H. abietis populations in managed forests. Full article

Significant market value discrepancies exist between clear and knotty Chinese fir (Cunninghamia lanceolata) wood, distinguished not only by their aesthetic variations but also by their distinct material properties. This study aimed to explore the differences in physical and mechanical properties between clear and knotty Chinese fir wood. Nine standard trees were chosen from a 26-year-old Chinese fir plantation for the experiment. Subsequent to felling, trunk segments below 7 m in length were transported to the laboratory. For each tree, detailed preparations were made to obtain clear and knotty wood specimens, and these distinct wood specimens were subjected to thorough physical and mechanical assessments. The results revealed significant variations in properties between clear and knotty Chinese fir wood. The shrinkage and swelling coefficients of knotty wood were generally lower than those of clear wood, except for higher radial and tangential air-dry shrinkage. Specifically, the swelling ratio of knotty wood was at least 0.40% lower, and the oven-dry shrinkage was at least 0.58% lower than that of clear wood. Knotty wood exhibited higher air-dry and oven-dry densities, with its density being at least 0.15 g cm⁻³ higher than that of clear wood. However, its mechanical properties, including tensile strength, compression strength, impact bending strength, bending strength, and modulus of elasticity, were lower than those of clear wood. For instance, the tensile strength parallel to the grain of clear wood was 40.63 MPa higher, the modulus of elasticity was 1595 MPa higher, and the impact bending strength was 27.12 kJ m⁻² greater than that of knotty wood. Although the tangential and radial surface hardness of knotty wood increased significantly compared to clear wood, the end hardness remained relatively lower. Overall, knotty Chinese fir wood displayed enhanced physical properties, whereas clear wood showcased superior mechanical properties. Careful selection between clear and knotty wood is recommended based on the specific requirements of wooden structural elements to optimize timber resource utilization. Full article

This study fabricated (Al₆₃Cu₂₅Fe₁₂)₉₉Ce₁ quasicrystal-enhanced aluminum matrix composites using the hot-pressing method to investigate their interfacial reaction traits. Microstructure analysis revealed that at 490 °C for 30 min of hot-pressing, the interface between the matrix and reinforcement was clear and intact. Chemical diffusion between the I-phase and aluminum matrix during sintering led to the formation of Al₇Cu₂Fe, AlFe, and AlCu phases, which, with their uniform and fine distribution, significantly enhanced the alloy’s overall properties. Regarding compactness, it first increased and then decreased with different holding times, reaching a maximum of about 98.89% at 490 °C for 30 min. Mechanical property analysis showed that compressive strength initially rose and then fell with increasing sintering temperature. After 30 min at 490 °C, the reinforcement particles and matrix were tightly combined and evenly distributed, with a maximum compressive strength of around 790 MPa. Additionally, the diffusion dynamics of the transition layer were simulated. The reaction rate of the reaction layer increased with hot-pressing temperature and decreased with holding time. Selecting a lower temperature and appropriate holding time can control the reaction layer thickness to obtain composites with excellent properties. This research innovatively contributes to the preparation and property study of such composites, providing a basis for their further application. Full article

Some biostimulant products provide proven benefits to plant production, potentially offering more environmentally friendly, sustainable, and natural inputs into production systems. However, the transference and predictability of known benefits between different growth environments, application protocols, and management systems are fraught with difficulty. In this study, we carried out carefully controlled glasshouse and in vitro assays with applications of humic acids, protein hydrolysates, and seaweed extract to compare the variability of biostimulant effects and dosage-dependent variations across diverse conditions, encompassing a sufficient range to comprehensively assess their full spectrum of impacts. The results demonstrated a clear trend of dosage-dependent effects with each biostimulant exhibiting a significant growth-promoting effect within a critical concentration range, but detrimental effects when the concentration fell outside this range. While substantial growth-promoting effects were observed under glasshouse conditions, biostimulant applications tended to be more sensitive and generally led to negative impacts in sterilised conditions. The combined use of biostimulants mostly resulted in detrimental and toxicological responses with only two combined treatments showing marginal synergistic effects. The findings demonstrated a complex interplay between biostimulants and the growth conditions of plants. Lack of knowledge of the indirect effects of different growth media may result in negative impacts of biostimulant applications and combinations of products outside narrow critical concentration ranges. Full article

Most studies on Chinese dormitories are carried out in summer, while few focus on a transition season or winter. This study evaluated the air quality of a student dormitory in a university in the Beijing area by using a questionnaire survey and on-site measurements. The CO₂ concentration was used as an indoor air quality evaluation index to characterize the freshness of the air, and different window opening conditions in the dormitory were simulated, with corresponding improvement plans proposed. The results of this study revealed that the air quality and thermal comfort of the student dormitories during a transition season and winter fell short of expectations. According to the survey, students who opened their windows frequently had a better subjective perception of the air quality. However, due to the large temperature difference between day and night, more than 80% of the students felt too cold when opening the windows. For daytime conditions, the area of unilateral ventilation window opening should not be less than 0.39 m², the area of bilateral ventilation window opening should not be less than 0.13 m², and the time taken to close the windows and doors should not exceed the maximum ventilation interval. Empirical equations were fitted for nighttime conditions based on the CO₂ concentration, number of people in the room, and window opening area, resulting in a reasonable window opening area of 0.349 m²~0.457 m². In sum, this study assessed the air quality status within typical university dormitories across varying seasons, gaining a clear understanding of how different ventilation strategies and occupant densities influence air freshness and thermal comfort. Based on these insights, a practical and optimized window area recommendation was formulated to enhance the indoor environmental quality in these dormitories. Full article

Pancreatic cancer (PC) ranks as the seventh leading cause of cancer-related deaths, with approximately 500,000 new cases reported in 2020. Existing strategies for early PC detection primarily target individuals at high risk of developing the disease. Nevertheless, there is a pressing need to identify innovative clinical approaches and personalized treatments for effective PC management. This study aimed to explore the dysbiosis signature of the fecal microbiota in PC and potential distinctions between its Intraductal papillary mucinous neoplasm (IPMN) and pancreatic ductal adenocarcinoma (PDAC) phenotypes, which could carry diagnostic significance. The study enrolled 33 participants, including 22 diagnosed with PDAC, 11 with IPMN, and 24 healthy controls. Fecal samples were collected and subjected to microbial diversity analysis across various taxonomic levels. The findings revealed elevated abundances of Firmicutes and Proteobacteria in PC patients, whereas healthy controls exhibited higher proportions of Bacteroidota. Both LEfSe and Random Forest analyses indicated the microbiome’s potential to effectively distinguish between PC and healthy control samples but fell short of differentiating between IPMN and PDAC samples. These results contribute to the current understanding of this challenging cancer type and highlight the applications of microbiome research. In essence, the study provides clear evidence of the gut microbiome’s capability to serve as a biomarker for PC detection, emphasizing the steps required for further differentiation among its diverse phenotypes. Full article

Understanding how thinning strategies impact wood quality and quantity for different purposes is of interest, given that plantation management is often based on parameters that require validation under varying growth conditions. Planted forests for solid purposes in the northern region of Urugay, western Argentina and South of Brazil are usually managed in initial stockings ranging from 800 to 1200 trees·ha⁻¹ depending on the use of clones or seeds. Subsequent thinnings are applied (at plantation ages varying from 3 to 11 years) up to final stockings of around 200 trees·ha⁻¹. This study evaluated contrasting thinning regimes applied early in the crop cycle, with an initial tree density of 840 trees·ha⁻¹. Two thinning treatments were applied at 1.5 and 7.3 years, reducing tree densities to 700–400 and 400–100 trees·ha⁻¹, respectively. Growth analyses were conducted from 1.5 to 20.8 years, considering total height, diameter at breast height, individual volume, total and commercial volume per hectare, mean annual increase, and current annual increase. At the final harvest, contrasting tree densities of 100, 250, and 400 trees·ha⁻¹ were sampled to assess wood density and mechanical properties (bending and compression on small-scale clear samples). Individual growth and wood properties were related to a Stand Density Index to understand the effect of competition on these values. The results identified thinning regimes that resulted in the most significant individual and per-hectare growth (both in thinning and clear felling) and the optimal harvest time under specific growth conditions. We assessed the proportions of commercial logs for sawmill and pulp uses, providing valuable inputs for subsequent economic analyses of thinning regimes aiming for the most convenient combination of wood products. Wood’s physical and mechanical properties were relatively little affected by contrasting levels of competition between trees; therefore, the choice of silvicultural system will depend on production and economic criteria. Full article

The assortment of low-cost 3D printers for “in-practice” use, e.g., for clear aligner therapies, is ever increasing. To address concerns about the accuracy of orthodontic models produced on such printers when cost-efficient modes of 3D printing are employed, this study examined the effect of print model body type and print angulation on accuracy. Six printing-configuration groups were included: two model types (solid or hollow shell) combined with three print angles (0°, 70°, or 90°) with 10 models/group; all models were printed with 100 µm layer thickness using a digital light processing-based three-dimensional printer. Eleven selected structures and distances were measured on the printed models with a digital microscope and compared to the same measures on a digitized master model. The clinically acceptable range was set at ±0.25 mm difference from the master model for single tooth measurements (intra-tooth) and ±0.5 mm for cross-arch measurements (inter-tooth). For individual measurements across all models, 98% fell within clinical acceptability. For mean measurements within each model group, only canine height for the shell-0° model had a mean difference (−0.26 mm ± 0.03) outside the clinically acceptable range for intra-tooth measurements. Standard deviations for all intra-tooth measurements were within 0.07 mm. While none of the mean inter-tooth measurements exceeded the acceptability range, the standard deviations were larger (0.04 to 0.30 mm). The accuracy of the orthodontic models for clear aligner therapies was not impacted beyond the clinically acceptable range when altering model body type and print angulation to improve efficiency of 3D printing. These findings suggest greater flexibility of the practitioner to alter print settings to address time and cost efficiency in various clinical scenarios and still maintain clinically acceptable model accuracy. Full article

A cut-to-length (CTL) forest harvester is a purpose-built, hydraulically actuated mobile work machine used for felling, delimbing, and cross-cutting trees into dimensions and assortments. In such a machine, the diesel engine load is known to fluctuate greatly over the work cycle. To manage these changing power demands, a hydraulic hybrid system is implemented into a full-scale proof-of-concept machine to discover its functionality and to collect experimental results in real operating conditions. The hydraulic hybrid system installed on a CTL harvester machine was tuned to enable the collection of two actual datasets of timber harvesting, and data collection over a period of production use was carried out. In addition to updating the state-of-the-art research into heavy non-road mobile machinery regarding hybrid systems, this paper discusses the two tests carried out in actual production conditions with the studied hydraulic hybrid system. The adaptations and modifications to the studied and tested system since earlier papers are discussed—especially the improvements in the system dynamics and response as well as the control of the hybrid system. In conclusion, the tested system can be said to operate satisfactorily and shows clear advantages over the conventional system, even though it still has some limitations. The results show that the system has the potential to increase the productivity of the machine through the demonstrated higher peak power, as well as showing improvement in fuel efficiency under highly fluctuating loads, especially with heavy or large-volume tree stems. Full article

The static bending properties of Scots pine (Pinus sylvestris L.) clear wood were studied using a material collected from commercial thinning forests in eastern Finland. In Myrtillus type, the modulus of elasticity and bending strength of the first thinning wood were 7.8 GPa and 66.0 MPa, respectively, whereas for more mature wood from the second thinnings, the modulus of elasticity and bending strength were 10.0 GPa and 80.3 MPa. The results were compared with final fellings, which resulted in the modulus of elasticity of 10.1 GPa and bending strength of 81.8 MPa. The bending properties of the first thinning material were low, and thus they did not indicate any potential for applications requiring high strength or stiffness and material homogeneity. On the contrary, the properties of Scots pine wood from the second commercial thinnings may be comparable with or sometimes even better than those of the final-felling wood. The results can be utilised in wood marketing, procurement, sorting, allocation to different industries and end-uses, as well as in wood processing, product sales, and branding. Full article

Forests are important ecosystems that face threats from climate change and global environmental shifts, with invasive alien plant species being a significant concern. Some of these invasive species have already become established, while others are in the process of naturalisation. Although forests are a relatively stable ecosystem, extreme weather events increase their vulnerability to change, and clearings left after natural disturbances are particularly susceptible to invasion by alien plant species (IAPS). We created risk maps of two species that have spread rapidly in the last decade: American pokeweed (Phytolacca americana) and the tree of heaven (Ailanthus altissima). We prepared a generalised linear model based on the occurrence data collected within the LIFE ARTEMIS project. Eleven environmental variables were used to determine habitat characteristics. We constructed two models for each species: one covering the entirety of Slovenia and the other specifically for the forested areas in Slovenia, with the latter incorporating forest-specific variables (such as forest sanitation felling and monocultures). We observed the presence of both species at lower altitudes and in close proximity to water sources. American pokeweed tends to occur nearer to railways, while the presence of the tree of heaven is associated with areas lacking carbonate parent material and influenced by land use patterns. In forested areas, the occurrence of American pokeweed is influenced by forest habitat characteristics, such as disturbances caused by extreme weather events or the prevalence of Norway spruce monocultures. In contrast, the occurrence of the tree of heaven is influenced by more general environmental variables, such as altitude and proximity to railways. Consequently, we have generated risk maps for the entirety of Slovenia and separately for forested areas, both of which indicate similar levels of risk, particularly for the tree of heaven. The risk map for American pokeweed highlights numerous vulnerable areas, especially forest edges, which are highly susceptible to invasion. Furthermore, there is a higher likelihood of this species occurring in areas that have undergone sanitation felling. This study suggests that the production of risk maps of IAPS could be improved by focussing on habitat types and taking into account habitat-specific variables. This approach could enhance the early detection and management of these invasive species. Full article