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Keywords = consumer preference

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20 pages, 2001 KB  
Article
Factors Influencing Courier Drivers’ Preferences and Safety Perceptions in Urban Deliveries
by Tijana Ivanišević, Aleksandar Trifunović, Larysa Neduzha and Sreten Simović
Logistics 2025, 9(4), 145; https://doi.org/10.3390/logistics9040145 - 13 Oct 2025
Abstract
Background: Urban freight transport is essential for the functioning of cities. The COVID-19 pandemic accelerated the growth of e-commerce, creating new challenges for courier services. While consumer satisfaction has been extensively studied, little attention has been paid to courier drivers’ own perceptions and [...] Read more.
Background: Urban freight transport is essential for the functioning of cities. The COVID-19 pandemic accelerated the growth of e-commerce, creating new challenges for courier services. While consumer satisfaction has been extensively studied, little attention has been paid to courier drivers’ own perceptions and preferences. This study aims to fill that gap. Methods: A questionnaire survey was conducted among 139 drivers employed in eight courier companies in Serbia. Data were analyzed using parametric statistical methods (Independent Samples T-Test, Paired-Samples T-Test, and One-way ANOVA), with additional post hoc tests to explore group differences. Results: Statistically significant differences were observed across demographic, operational, and safety-related factors (gender, age, residence, occupation, license ownership, delivery area, and type of goods). A strong preference emerged for passenger vehicles as the safest mode of delivery, highlighting a misalignment between current operational practices and drivers’ safety perceptions. Conclusions: The findings emphasize the importance of tailoring delivery strategies to demographic and operational contexts. Practical recommendations include improving transport safety, optimizing delivery zones, and addressing driver satisfaction as a determinant of service quality. The study contributes new insights into last-mile delivery by focusing on the perspectives of courier drivers rather than consumers. Full article
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30 pages, 531 KB  
Article
Differences in Online Consumer Behavior: A Multi-Dimensional Comparative Study in the Context of European Digital Commerce
by Radovan Madlenak, Roman Chinoracky, Natalia Stalmasekova and Lucia Madlenakova
Behav. Sci. 2025, 15(10), 1384; https://doi.org/10.3390/bs15101384 - 12 Oct 2025
Abstract
The aim of this study was to analyze international differences in online consumer behavior. The analysis was carried out on a sample of 763 participants from the countries of Spain, France, Poland and Russia. Online consumer behavior was examined from the perspective of [...] Read more.
The aim of this study was to analyze international differences in online consumer behavior. The analysis was carried out on a sample of 763 participants from the countries of Spain, France, Poland and Russia. Online consumer behavior was examined from the perspective of seven dimensions: shipping-related concerns and preferences, price sensitivity and perceived cost advantage, quality perception, security concerns, time-related benefits, availability and quality of information, and shopping service satisfaction. Data were verified using Average inter-item correlation, the Shapiro–Wilk test and Levene Statistic. Subsequently, Welch’s ANOVA and one-way ANOVA and the Games–Howell and Tukey HSD post hoc tests were applied. Statistically significant differences were fully identified in all examined dimensions. The largest differences were recorded in price sensitivity, shipping-related concerns and security concerns. The effect measurements, in addition to ANOVA and post hoc tests, confirm the significance of these differences. National context, shaped by culture, institutional trust and digital infrastructure, continues to influence online consumer behavior. The strategies that the businesses should adopt should focus on approaches that are tailor-made for a specific market. This means that adapting pricing models, strengthening trust (e.g., through secure payments and strengthening safe return policies), and adapting delivery options to local preferences can lead to improved customer satisfaction in cross-border e-commerce. Full article
(This article belongs to the Special Issue Exploring the Dynamics of Consumer Behavior in Digital Commerce)
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20 pages, 2113 KB  
Article
Evaluating the Influence of Two Different Red Wines on the Physicochemical Properties, Volatile Compound Profiles, and Sensory Attributes of Wine-Soaked Pressed Cheeses
by Paulina Freire, Daniel Olmos, Miguel A. Pedroza, Jack Adamson, Reem Elkhalil, Madison Atwood, Justin P. Miller-Schulze and Carmen C. Licon
Foods 2025, 14(20), 3475; https://doi.org/10.3390/foods14203475 (registering DOI) - 12 Oct 2025
Abstract
This study evaluated the effects of wine-soaking on cow’s milk pressed cheese properties and developed a standardized cheesemaking procedure. Cheese was soaked in Cabernet Sauvignon and Alicante Bouschet red wines for two soaking periods of four days after the brining process. The physicochemical, [...] Read more.
This study evaluated the effects of wine-soaking on cow’s milk pressed cheese properties and developed a standardized cheesemaking procedure. Cheese was soaked in Cabernet Sauvignon and Alicante Bouschet red wines for two soaking periods of four days after the brining process. The physicochemical, microbiological, and volatile composition were evaluated, along with consumer sensory evaluation. After 60 days of ripening, wine-soaked cheeses had statistically lower salt and moisture levels, with higher protein and fat content than the unsoaked cheeses. Alicante Bouschet cheeses have a darker purple-red color than Cabernet Sauvignon. The microbiological analysis found no significant differences across treatments and samplings. The most representative volatile compounds in wine-soaked cheeses were esters and ketones. Principal Components Analysis on the volatile compounds showed a clear separation between the two wine-soaked cheeses and the control cheese. For example, Cabernet-soaked cheese had higher levels of phenylethyl alcohol and 2-phenylethyl acetate (floral aromas), while Alicante-soaked cheese was distinguished by nonanal (fruity and grassy aroma). Sensory results showed preferences for the overall liking, flavor, and rind color for the wine-soaked cheeses over the control. Consequently, a standardized recipe for wine-soaked pressed cheese was developed, along with specific parameters for the soaking process to ensure a well-received product. Full article
(This article belongs to the Section Dairy)
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23 pages, 1547 KB  
Article
Roles, Risks and Responsibility: Foundations of Pro-Environmental Culture in Everyday Choices
by Olena Pavlova, Oksana Liashenko, Olena Mykhailovska, Kostiantyn Pavlov, Krzysztof Posłuszny and Antoni Korcyl
Sustainability 2025, 17(20), 9019; https://doi.org/10.3390/su17209019 (registering DOI) - 11 Oct 2025
Abstract
This study explores how contextual framings influence sustainable decision-making in everyday situations. Building on the literature about the intention–behaviour gap, we examine the combined effect of role activation and environmental risk on pro-environmental preferences. A scenario-based behavioural experiment, conducted via oTree, integrated within-subject [...] Read more.
This study explores how contextual framings influence sustainable decision-making in everyday situations. Building on the literature about the intention–behaviour gap, we examine the combined effect of role activation and environmental risk on pro-environmental preferences. A scenario-based behavioural experiment, conducted via oTree, integrated within-subject role framing (citizen, consumer, neutral) with randomised environmental risk conditions. Participants completed repeated binary choice tasks, where Eco-Preference was defined as the frequency with which they chose the sustainable option. The results indicate that activating a citizen role significantly increased Eco-Preference compared to consumer or neutral framings, while high-risk contexts did not directly boost sustainable behaviour. Instead, risk cues had an indirect effect through motivational states, highlighting the mediating role of Eco-Preference. Theoretically, this study advances Eco-Preference as a latent behavioural construct linking identity-based theories of responsibility with decision-based models of sustainability. Practically, the findings underscore the potential of role-based communication strategies to enhance ecological responsibility, suggesting that both policy and organisational interventions can benefit from fostering civic identities. Ultimately, the framework is applicable across cultures by offering a behavioural measure less prone to survey bias, supporting future comparative research on environmental decision-making. Full article
(This article belongs to the Special Issue Quality of Life in the Context of Sustainable Development)
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30 pages, 2049 KB  
Article
From Sea to Table: The Role of Traceability in Italian Seafood Consumption
by Simona Violino, Damianos Chatzievangelou, Giulio Sperandio, Simone Gaetano Amato, Chiara Fini, Domenico Ciorciaro, Simone Figorilli, Cecilia Ripa, Simone Vasta, Francesca Antonucci, Federico Pallottino, Raffaele De Luca, Daniela Scutaru, Sara Biancardi, Elisa Pignoni and Corrado Costa
Foods 2025, 14(20), 3469; https://doi.org/10.3390/foods14203469 (registering DOI) - 11 Oct 2025
Viewed by 54
Abstract
Seafood plays a key role in a healthy diet due to its high content of essential nutrients. However, its global trade and complex supply chains expose it to frequent mislabeling and food fraud. This study investigates Italian consumers’ willingness to pay (WTP) for [...] Read more.
Seafood plays a key role in a healthy diet due to its high content of essential nutrients. However, its global trade and complex supply chains expose it to frequent mislabeling and food fraud. This study investigates Italian consumers’ willingness to pay (WTP) for traceable seafood products, exploring how blockchain technology (BT) can enhance transparency and economic sustainability in the fish supply chain. An online questionnaire, administered in 2022 and 2024, gathered responses from a diverse demographic, focusing on four representative seafood species: farmed sea bass, striped venus clams, giant red shrimp, and albacore tuna. Results indicate that most respondents—primarily with higher education levels—value traceability and are willing to pay a premium for certified, traceable seafood. The study models the economic feasibility of implementing BT at both national and regional levels and finds that the consumer’s WTP exceeds the additional costs incurred by adopting BT. These findings support the viability of traceability systems in improving food safety and sustainability, while reinforcing consumer trust. The results also underscore the importance of providing clear information at the point of purchase, particularly regarding species, origin, and production methods—factors critical to informed seafood choices and advancing more sustainable consumer behavior in Italy. Full article
(This article belongs to the Section Foods of Marine Origin)
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15 pages, 1567 KB  
Article
Chemical and Sensory Attributes of Different Ethanol Reduction Methods in Muscadine Wine Production
by Alexandra A. Escalera, Patricia C. Patricio Morillo, Drew Budner, Katherine A. Thompson-Witrick and Andrew J. MacIntosh
Beverages 2025, 11(5), 146; https://doi.org/10.3390/beverages11050146 - 9 Oct 2025
Viewed by 135
Abstract
There has been a recent shift in the global wine market towards reduced-alcohol wines. Muscadine grapes (Vitis rotundifolia) have become a popular choice in many emerging markets; however, their suitability in reduced-alcohol wine production has not been extensively tested. In this [...] Read more.
There has been a recent shift in the global wine market towards reduced-alcohol wines. Muscadine grapes (Vitis rotundifolia) have become a popular choice in many emerging markets; however, their suitability in reduced-alcohol wine production has not been extensively tested. In this study, methods to reduce ethanol in muscadine wine were compared to determine differences in chemical and sensory attributes and consumer preference. The methods evaluated included full fermentation time with Saccharomyces cerevisiae (control), reduced fermentation time with Saccharomyces cerevisiae (stopped fermentation), fermentation with Saccharomycodes ludwigii yeast (instead of Saccharomyces cerevisiae), and vacuum distillation. The control and distilled wines were fermented for 121 h, Saccharomycodes ludwigii for 45 h, and the stopped fermentation wine for 3 h. Yeast and sugar levels were monitored throughout the fermentation processes using brix measurements and yeast counts. After the fermentation, the color, pH, volatiles, and titratable acidity (TA) were measured. The results showed that Saccharomycodes ludwigii fermented more slowly than Saccharomyces cerevisiae, and that both the stopped fermentation and Saccharomycodes ludwigii wines had lower titratable acidity with a more intense color. The total concentration of volatile compounds for the Saccharomycodes ludwigii wine and the stopped wine were lower than for the distilled and control wines. A consumer panel (n = 92) judged the wine samples on chemical qualities and overall preference. The distilled wine was perceived as more alcoholic compared to the other reduced-alcohol wines. The results showed that the stopped fermentation and Saccharomycodes ludwigii wines were preferred by consumers over the control and vacuum-distilled wines. Full article
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32 pages, 3312 KB  
Article
Green Investment and Emission Reduction in Supply Chains Under Dual-Carbon Regulation: A Dynamic Game Perspective on Coordination Mechanisms and Policy Insights
by Dandan Wu, Kun Li and Yang Cheng
Sustainability 2025, 17(19), 8951; https://doi.org/10.3390/su17198951 - 9 Oct 2025
Viewed by 217
Abstract
This study examines green investment and emission reduction strategies in a two-tier supply chain under dual-carbon regulation that combines a carbon tax with a cap-and-trade mechanism. A multi-stage dynamic game model is developed, in which the manufacturer reduces emissions through recycling efforts and [...] Read more.
This study examines green investment and emission reduction strategies in a two-tier supply chain under dual-carbon regulation that combines a carbon tax with a cap-and-trade mechanism. A multi-stage dynamic game model is developed, in which the manufacturer reduces emissions through recycling efforts and investments in green technology. We compare optimal decisions under centralized, decentralized, and coordinated structures, and propose an enhanced bilateral cost-sharing contract to improve collaboration. Numerical experiments validate the theoretical results, and sensitivity analyses provide further insights. The results show that while both carbon tax and permit trading increase emission reduction, the carbon tax may lower manufacturer profit, underscoring the need for coordinated policy design. Benchmarking proves more effective than grandfathering in stimulating green investment, particularly under high carbon prices and strong consumer environmental preferences. The proposed contract alleviates free riding, enhances overall supply chain profitability, and improves emission reduction performance. Policy implications highlight the importance of prioritizing benchmark allocation, promoting consumer environmental awareness, and encouraging firms to integrate carbon asset management with technological innovation. This research provides both theoretical and practical insights for designing effective carbon policies and collaborative mechanisms in green supply chains. Full article
(This article belongs to the Special Issue Sustainable Operations and Green Supply Chain)
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20 pages, 2201 KB  
Article
Carob Pulp Flour as a Sustainable and Functional Ingredient in the Bakery: Effects of Leavening Typologies on Dough and Bread Properties
by Sebastiano Rosati, Ilenia Gaeta, Lucia Maiuro, Maria Carmela Trivisonno, Maria Cristina Messia and Elena Sorrentino
Life 2025, 15(10), 1571; https://doi.org/10.3390/life15101571 - 8 Oct 2025
Viewed by 218
Abstract
Carob pulp flour (Ceratonia siliqua) is gaining attention as a sustainable ingredient with nutritional and functional potential. This study evaluated the partial replacement of soft wheat flour with 10% carob pulp flour in breadmaking, focusing on the role of different leavening [...] Read more.
Carob pulp flour (Ceratonia siliqua) is gaining attention as a sustainable ingredient with nutritional and functional potential. This study evaluated the partial replacement of soft wheat flour with 10% carob pulp flour in breadmaking, focusing on the role of different leavening strategies: commercial baker’s yeast (LB), a selected starter culture, Lactiplantibacillus plantarum SL31 and Saccharomyces cerevisiae SY17 (LI), and a type I sourdough (LS). Dough rheology, microbial dynamics, bread quality, acceptability, and shelf-life were assessed. Results showed that the inclusion of carob pulp flour enhances the nutritional profile while maintaining satisfactory technological performance. The leavening strategy strongly influenced the final products: breads made with commercial yeast displayed high volume and softness but were less stable during storage; LS breads achieved greater microbial stability but were limited by excessive acidity and reduced sensory acceptance; breads obtained with the selected starter culture offered the most balanced outcome, combining moderate structure with enhanced flavor and consumer preference. Overall, the findings demonstrate the feasibility of incorporating carob pulp flour into bakery products and highlight the potential of tailored starter cultures as a promising compromise between technological performance, sensory quality, and shelf-life. Future work should optimize fermentation approaches to further enhance consumer appeal and support industrial application. Full article
(This article belongs to the Section Life Sciences)
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25 pages, 2622 KB  
Article
Food Emotional Perception and Eating Willingness Under Different Lighting Colors: A Preliminary Study Based on Consumer Facial Expression Analysis
by Yuan Shu, Huixian Gao, Yihan Wang and Yangyang Wei
Foods 2025, 14(19), 3440; https://doi.org/10.3390/foods14193440 - 8 Oct 2025
Viewed by 341
Abstract
The influence of lighting color on food is a multidimensional process, linking visual interventions with people’s perception of food appearance, physiological responses, and psychological associations. This study, as a preliminary exploratory research, aims to initially investigate the effects of different lighting colors on [...] Read more.
The influence of lighting color on food is a multidimensional process, linking visual interventions with people’s perception of food appearance, physiological responses, and psychological associations. This study, as a preliminary exploratory research, aims to initially investigate the effects of different lighting colors on food-induced consumer appetite and emotional perception. By measuring consumers’ physiological facial expression data, we verify whether the results are consistent with self-reported subjective evaluations. Questionnaires, Shapiro–Wilk tests, and one-sample t-tests were employed for data mining and cross-validation and combined with generalized facial expression recognition (GFER) technology to analyze participants’ emotional perceptions under various lighting colors. The results show that consumers displayed the most positive emotions and the highest appetite under 2700 K warm white light. Under this condition, the average intensity of participants’ “happy” emotion was 0.25 (SD = 0.12), indicating a clear positive emotional state. Eating willingness also reached its peak at 2700 K. In contrast, blue light-induced negative emotions and lower appetite. Among all lighting types, blue light evoked the strongest “sad” emotion (M = 0.39). This study provides a preliminary exploration of the theoretical framework regarding the relationship between food and consumer behavior, offering new perspectives for product marketing in the food industry and consumer food preference cognition. However, the generalizability of its conclusions still requires further verification in subsequent studies. Full article
(This article belongs to the Section Sensory and Consumer Sciences)
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30 pages, 1660 KB  
Article
Network Equilibrium Analysis of Dual-Channel Environmental Hotel Supply Chains with M&A Using Variational Inequalities
by Preeyanuch Chuasuk and Shinawat Horayangkura
Sustainability 2025, 17(19), 8913; https://doi.org/10.3390/su17198913 - 8 Oct 2025
Viewed by 188
Abstract
This paper develops a dual-channel environmental hotel supply chain equilibrium model to investigate the impact of mergers and acquisitions (M&A), decision makers’ altruistic preferences, and consumers’ low-carbon preferences under demand uncertainty. The model incorporates hotels, online travel agency (OTA) platforms, and demand markets [...] Read more.
This paper develops a dual-channel environmental hotel supply chain equilibrium model to investigate the impact of mergers and acquisitions (M&A), decision makers’ altruistic preferences, and consumers’ low-carbon preferences under demand uncertainty. The model incorporates hotels, online travel agency (OTA) platforms, and demand markets and captures interactions under both the merchant and agency models. Variational inequalities are employed to describe interdependent decision-making behaviors, and the projection gradient algorithm is used to obtain equilibrium solutions. The numerical analysis reveals that sustainability factors directly affect the profitability of both hotels and OTAs. Under the agency model, higher commission rates reduce hotel profits while significantly increasing OTA gains. When comparing the merchant and agency models without M&A, the merchant model yields higher hotel profits, whereas the agency model favors OTAs. Furthermore, when M&A is combined with altruistic preferences, the utility of hotels and OTAs under the agency model reaches the highest level, while M&A offers limited advantages for OTAs. Overall, the findings highlight that sustainability factors, altruistic preferences, and M&A jointly interact with merchant and agency governance structures to shape profitability and utility. These results provide theoretical insights and managerial implications for developing resilient, cooperative, and low-carbon hotel supply chains under demand uncertainty. Full article
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18 pages, 4521 KB  
Article
Lightweight Design and Research of Electric Towing Winch Based on Kriging-NSGA-III-TOPSIS Multi-Objective Optimization Technology
by Quanliang Liu, Lu Feng, Ya Wang, Ji Lin and Linsen Zhu
Machines 2025, 13(10), 922; https://doi.org/10.3390/machines13100922 - 6 Oct 2025
Viewed by 204
Abstract
To address the challenges of weight redundancy, low material utilization, and excessive performance margins in the design of electric cable-hauling machines, this study proposes a novel multi-objective optimization framework. The framework integrates Latin hypercube experimental design, Kriging surrogate modeling, a Non-dominated Sorting Genetic [...] Read more.
To address the challenges of weight redundancy, low material utilization, and excessive performance margins in the design of electric cable-hauling machines, this study proposes a novel multi-objective optimization framework. The framework integrates Latin hypercube experimental design, Kriging surrogate modeling, a Non-dominated Sorting Genetic Algorithm III (NSGA-III), and a coupled TOPSIS (Technique for Order Preference by Similarity to Ideal Solution) approach. A high-fidelity finite element model based on extreme operating conditions was established to simulate the performance of the electric towing winch. The Kriging model was employed to replace time-consuming finite element calculations, significantly improving computational efficiency. The NSGA-III algorithm was then utilized to search for the Pareto front, identifying a set of optimal solutions that balance multiple design objectives. Finally, the TOPSIS method was applied to select the most preferable solution from the Pareto front. The results demonstrate a 7.32% reduction in the overall mass of the towing winch, a 7.34% increase in the safety factor, and a 4.57% reduction in maximum structural deformation under extreme operating conditions. These findings validate the effectiveness of the proposed Kriging-NSGA-III-TOPSIS strategy for lightweight design of ship deck winch machinery. Full article
(This article belongs to the Section Machine Design and Theory)
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24 pages, 1545 KB  
Article
AI-Driven Privacy Trade-Offs in Digital News Content: Consumer Perception of Personalized Advertising and Dynamic Paywall
by Jae Woo Shin
Journal. Media 2025, 6(4), 170; https://doi.org/10.3390/journalmedia6040170 - 6 Oct 2025
Viewed by 455
Abstract
As digital media companies pursue sustainable revenue, AI-based strategies like personalized advertising and dynamic paywalls have become prevalent. These monetization models involve different forms of consumer data collection, raising distinct privacy concerns. This study investigates how digital news users perceive privacy trade-offs between [...] Read more.
As digital media companies pursue sustainable revenue, AI-based strategies like personalized advertising and dynamic paywalls have become prevalent. These monetization models involve different forms of consumer data collection, raising distinct privacy concerns. This study investigates how digital news users perceive privacy trade-offs between these two AI-driven models. Based on Communication Privacy Management Theory and Privacy Calculus Theory, we conducted a survey of 336 Korean news consumers. Findings indicate that perceived control and risk significantly affect users’ willingness to disclose data. Moreover, users with different privacy orientations prefer different monetization models. Those favoring dynamic paywalls tend to be more privacy-sensitive and show a higher willingness to pay for personalized, ad-free content. While personalization benefits are broadly acknowledged, the effectiveness of privacy control mechanisms remains limited. These insights highlight the importance of ethical, user-centered AI monetization strategies in journalism and contribute to theoretical discussions around algorithmic personalization and digital news consumption. Full article
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20 pages, 3013 KB  
Article
Exploring Static Biological Aging as a Method for Producing Low-Alcohol ‘Fino’ Type White Wines
by Raquel Muñoz-Castells, Lourdes Vega-Espinar, Juan Carlos García-García, Maria Trinidad Alcalá-Jiménez, Jaime Moreno-García, Cristina Lasanta and Juan Moreno
Fermentation 2025, 11(10), 575; https://doi.org/10.3390/fermentation11100575 - 5 Oct 2025
Viewed by 505
Abstract
Spanish “Fino”-style white wines are traditionally aged by a dynamic process under a flor veil of Saccharomyces cerevisiae, requiring ≥15% (v/v) ethanol, which is typically achieved through fortification. Market demand for lower-alcohol wines and the need to reduce [...] Read more.
Spanish “Fino”-style white wines are traditionally aged by a dynamic process under a flor veil of Saccharomyces cerevisiae, requiring ≥15% (v/v) ethanol, which is typically achieved through fortification. Market demand for lower-alcohol wines and the need to reduce production costs have encouraged the development of alternative approaches. Here, static biological aging was evaluated as a method for producing Fino-type wines with reduced ethanol content. Base wines with ~14% and ~15% (v/v) ethanol were aged for nine months, during which chemical, microbiological, and sensory parameters were analyzed, along with flor veil activity. Lower-ethanol wines showed greater flor activity, with approximately 20 more yeast isolates in the wines with 14% (v/v) ethanol. Higher acetaldehyde levels were detected in these wines, reaching about 377 mg L−1 compared to 230 mg L−1 in the control wines (≥15% v/v ethanol). Significant changes were observed in pH (3.13–3.47 vs. 3.04–3.46), volatile acidity (0.20–0.26 g L−1 vs. 0.31–0.66 g L−1), and several volatile compounds, resulting in chemical and sensory profiles consistent with traditional biologically aged wine. Static biological aging can yield lower-alcohol Fino-style white wines with sensory and chemical attributes comparable to the traditional fortified versions, providing a cost-effective alternative that aligns with evolving consumer preferences. Full article
(This article belongs to the Special Issue Scale-Up Challenges in Microbial Fermentation)
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21 pages, 1404 KB  
Article
Biscuits with Oleogels and Green Tea Extract Addition––Sensory Evaluation and Consumer Perception
by Sylwia Onacik-Gür, Anna Zbikowska, Iwona Szymańska and Małgorzata Kowalska
Appl. Sci. 2025, 15(19), 10728; https://doi.org/10.3390/app151910728 - 5 Oct 2025
Viewed by 371
Abstract
Oleogels are a new trend in food technology aimed at enhancing the nutritional value of products that contain solid fats. Solid fats are rich in nutritionally unfavorable saturated fatty acids (FAs), but oleogels (lipid systems derived from oleogelator and oils) are in unsaturated [...] Read more.
Oleogels are a new trend in food technology aimed at enhancing the nutritional value of products that contain solid fats. Solid fats are rich in nutritionally unfavorable saturated fatty acids (FAs), but oleogels (lipid systems derived from oleogelator and oils) are in unsaturated FAs. The use of oils rich in oxidation-prone unsaturated FAs in pastry products requires the use of an antioxidant, such as green tea extract (GTE). However, new products with altered composition should also be acceptable to consumers. The purpose of this study was to carry out a sensory evaluation of short-dough biscuits obtained with 1% of GTE and high-oleic oleogels and to find what may influence consumers’ anticipated perception. Products with monoacylglycerols (MAG) and candelilla wax (CLX) oleogels and non-gelled high-oleic rapeseed oil (HORO) were found to have equal or better sensory quality compared to products with traditional baker’s (palm) fat (PF). In terms of sensory attributes, products with ethyl cellulose oleogels were the most distinct from the other biscuits. Their sensory qualities were significantly lower, with a more noticeable rancid odor and taste. Products with CLX and MAG oleogels were more preferred than those with PF, as consumers were more likely to purchase them based on sensory impressions. Information about the presence of GTE and HORO in the formulation was shown to affect the perception of the product. Similarly, the information about the low saturated fat content encouraged consumers to purchase such products. Based on this, it can be concluded that displaying this type of information can help educate consumers, support better decision-making, and promote the selection of more nutritious options. Full article
(This article belongs to the Section Food Science and Technology)
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22 pages, 1222 KB  
Article
Codon Usage Bias of the Polyphenol Oxidase Genes in Camellia sinensis: A Comprehensive Analysis
by Yeşim Aktürk Dizman
Plants 2025, 14(19), 3074; https://doi.org/10.3390/plants14193074 - 4 Oct 2025
Viewed by 389
Abstract
Tea, a widely consumed beverage globally, is a vital agricultural product for many countries. Polyphenol oxidases (PPOs), copper-containing enzymes found in plants, fungi, and animals, are essential for physiological metabolism and enzymatic browning in tea plants (Camellia sinensis). Codon usage bias [...] Read more.
Tea, a widely consumed beverage globally, is a vital agricultural product for many countries. Polyphenol oxidases (PPOs), copper-containing enzymes found in plants, fungi, and animals, are essential for physiological metabolism and enzymatic browning in tea plants (Camellia sinensis). Codon usage bias (CUB), a key evolutionary characteristic, offers valuable insights into species evolution and gene function. However, the codon usage patterns of Camellia sinensis polyphenol oxidase (CsPPO) genes remain undocumented. In this study, we conducted, for the first time, a comprehensive analysis of CUB in 24 CsPPO genes, comparing their CUB profiles with those of other Camellia species (Camellia lanceoleosa, Camellia nitidissima, Camellia ptilophylla) and non-Camellia species (Actinidia chinensis, Cornus florida, Rhododendron vialii) to elucidate potential evolutionary relationships and functional constraints influencing CUB. Nucleotide composition analysis revealed an AT-rich bias, with a preference for G/C-ending codons at the third position. Codon usage indices indicated low expression levels and weak CUB. RSCU and RFSC analyses revealed that the preferred and high-frequency codons were mostly G/C-ending. Codon usage frequency analysis suggested Zea mays as a suitable host for CsPPO gene expression. ENC-GC3s, PR2, and neutrality plots showed natural selection had a stronger impact than mutation on CUB. Additionally, measure independent of length and composition (MILC) values confirmed low PPO gene expression levels, and correlation analyses demonstrated that both nucleotide composition and gene expression affect CUB. Overall, codon usage in CsPPO genes is mainly shaped by natural selection, with weak bias and low expression potential, providing useful insights for future genetic engineering and heterologous expression. Full article
(This article belongs to the Special Issue Plant Genetic Diversity and Molecular Evolution)
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