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Keywords = continuous-flow separation

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19 pages, 1890 KB  
Article
Mathematical Modeling of a Continuous Multistage Ethanol Production Bioprocess on an Industrial Scale
by Samuel C. Oliveira, Rafael H. Gonçalves and Ivan Ilich Kerbauy Veloso
Biomass 2025, 5(4), 65; https://doi.org/10.3390/biomass5040065 - 20 Oct 2025
Viewed by 193
Abstract
In this study, a mathematical model was proposed for a continuous, multistage, industrial-scale alcoholic fermentation process, comprising four vats in series with volumes equal to 600 m3, with separation, acid treatment, and cell recycling from the fourth to the first vat. [...] Read more.
In this study, a mathematical model was proposed for a continuous, multistage, industrial-scale alcoholic fermentation process, comprising four vats in series with volumes equal to 600 m3, with separation, acid treatment, and cell recycling from the fourth to the first vat. The system was operated daily under variable volumetric flow rates and substrate concentrations in the feed stream, i.e., F0 = 93–127 m3/h and S0 = 210–238 g/L. The mathematical model consisted of mass balance equations for cells, substrate, and product in the vats, the separator, and the acid treatment unit. An unsegregated and unstructured approach was used to describe the microbial population, with the kinetics of cell growth, substrate consumption, and product formation represented by equations generally adopted for alcoholic fermentation. The model parameters were estimated by nonlinear regression, providing typical values for alcoholic fermentation. Model predictions agreed well with both the experimental data used in the parameter estimation step and those used in the model validation step. Full article
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26 pages, 12326 KB  
Article
A Study on Energy Loss and Transient Flow Characteristics of a Large Volute Centrifugal Pump During Power-Off Process Under Cavitation Conditions
by Qingzhao Pang, Desheng Zhang, Gang Yang, Xi Shen, Qiang Pan, Linlin Geng and Qinghui Lu
J. Mar. Sci. Eng. 2025, 13(10), 1973; https://doi.org/10.3390/jmse13101973 - 15 Oct 2025
Viewed by 288
Abstract
A novel pumped storage system using centrifugal pumps to transfer water between reservoirs in coastal hydropower plants has significantly mitigated grid instability. However, frequent start–stop operations of large vertical centrifugal pumps, which serve as the core equipment, severely affect the operational stability of [...] Read more.
A novel pumped storage system using centrifugal pumps to transfer water between reservoirs in coastal hydropower plants has significantly mitigated grid instability. However, frequent start–stop operations of large vertical centrifugal pumps, which serve as the core equipment, severely affect the operational stability of these systems. In this study, the intrinsic connection between the cavitating flow field and irreversible losses during the process was analyzed using the entropy production theory. The time–frequency characteristics of pressure pulsation in pump were analyzed by using the continuous wavelet transform. The results indicate that with the reduction in the flow rate and rotational speed, the sheet cavitation at the impeller inlet rapidly weakens until it vanishes. The cavity cavitation within the draft tube commences to emerge in the turbine mode. Separation vortices are formed due to the mismatch in the flow angle at the impeller outlet. These vortices induce local cavitation, causing both a rapid energy loss increase and high-amplitude, low-frequency pressure pulsations. During transient processes, flow instabilities induce high-amplitude, low-frequency pressure pulsations within the stay vane region, with maximum amplitude attained during runaway condition. The research results provide a theoretical foundation for the stable operation of centrifugal pumps. Full article
(This article belongs to the Section Ocean Engineering)
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30 pages, 23274 KB  
Article
Unsteady Hydrodynamic Analysis and Experimental Methodology for Voith Schneider Propeller
by Wentao Liu, Zhihua Liu, Weixin Xue and Qian Chen
J. Mar. Sci. Eng. 2025, 13(10), 1933; https://doi.org/10.3390/jmse13101933 - 9 Oct 2025
Viewed by 247
Abstract
The Voith Schneider Propeller (VSP) operates with blades undergoing an approximately sinusoidal periodic motion along a circular path. Hydrodynamically, the continuous significant variation in the angle of attack between the blades and incoming flow, together with additional inertial effects caused by accelerated rotation, [...] Read more.
The Voith Schneider Propeller (VSP) operates with blades undergoing an approximately sinusoidal periodic motion along a circular path. Hydrodynamically, the continuous significant variation in the angle of attack between the blades and incoming flow, together with additional inertial effects caused by accelerated rotation, complicates the computation and measurement of hydrodynamic performance. To investigate the unsteady hydrodynamic behavior resulting from this coupled motion, a numerical model incorporating adaptive mesh refinement was developed to simulate VSP performance. Based on insights into the interaction between blade motion and hydrodynamics, an experimental platform was designed using servo motors to achieve precise synchronized blade control, enabling mutual validation between numerical simulations and transient hydrodynamic measurements. Results demonstrate that the coupled blade motion induces nonlinear variations in hydrodynamic forces. Rotational power loss limits VSP efficiency, and a negative thrust regime occurs at high advance coefficients. Rapid blade flipping leads to flow separation, identified as the primary cause of nonlinear lateral forces. The consistency between numerical and experimental results provides reliable data supporting theoretical studies. These findings offer valuable insights for optimizing motion control strategies in cycloidal propeller applications. Full article
(This article belongs to the Section Ocean Engineering)
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11 pages, 1669 KB  
Article
From Filamentous Bulking to Utilization: Formation Mechanisms of Filamentous Biofilms and Construction of Stabilized Systems
by Tao Song, Ji Li and Xiaolei Zhang
Water 2025, 17(19), 2885; https://doi.org/10.3390/w17192885 - 3 Oct 2025
Viewed by 424
Abstract
Sludge bulking in wastewater treatment is often caused by massive filamentous bacteria. This study aimed to turn such bacteria into a stable system dominated by filamentous biofilms (FBs) by using a continuous flow reactor (CFR) fed with simulated domestic wastewater; to address FBs’ [...] Read more.
Sludge bulking in wastewater treatment is often caused by massive filamentous bacteria. This study aimed to turn such bacteria into a stable system dominated by filamentous biofilms (FBs) by using a continuous flow reactor (CFR) fed with simulated domestic wastewater; to address FBs’ poor solid–liquid separation and uncontrollable sludge retention time (SRT), string carriers were added, SRT was controlled at 30 days, and parameters like mixed liquid suspended solids (MLSS) and sludge volume index (SVI) were monitored. Results showed filamentous Sphaerotilus (68–93% of FBs) self-aggregated as FBs’ reticular skeleton (loose, porous, stable, max 8 cm) with non-filamentous bacteria anchoring; FBs achieved >80% COD/NH4+-N removal despite low MLSS (<1000 mg/L) and SVI > 350 mL/g. The application of carriers increased the proportion of non-filamentous microorganisms to over 80%, reduced SVI to 150–400 mL/g, and increased MLSS to over 2700 mg/L, enabling stable operation. This study challenges the traditional negative perception of filamentous bacteria and opens new prospects for wastewater treatment technology. Full article
(This article belongs to the Section Wastewater Treatment and Reuse)
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16 pages, 2181 KB  
Article
Continuous Separation of Lithium Iron Phosphate and Graphite Microparticles via Coupled Electric and Magnetic Fields
by Wenbo Liu, Xiaolei Chen, Pengfei Qi, Xiaomin Liu and Yan Wang
Micromachines 2025, 16(10), 1094; https://doi.org/10.3390/mi16101094 - 26 Sep 2025
Viewed by 359
Abstract
Driven by the growing demand for sustainable resource utilization, the recovery of valuable constituents from spent lithium-ion batteries (LIBs) has attracted considerable attention, whereas conventional recycling processes remain energy-intensive, inefficient, and environmentally detrimental. Herein, an efficient and environmentally benign separation strategy integrating dielectrophoresis [...] Read more.
Driven by the growing demand for sustainable resource utilization, the recovery of valuable constituents from spent lithium-ion batteries (LIBs) has attracted considerable attention, whereas conventional recycling processes remain energy-intensive, inefficient, and environmentally detrimental. Herein, an efficient and environmentally benign separation strategy integrating dielectrophoresis (DEP) and magnetophoresis (MAP) is proposed for isolating the primary components of “black mass” from spent LIBs, i.e., lithium iron phosphate (LFP) and graphite microparticles. A coupled electric–magnetic–fluid dynamic model is established to predict particle motion behavior, and a custom-designed microparticle separator is developed for continuous LFP–graphite separation. Numerical simulations are performed to analyze microparticle trajectories under mutual effects of DEP and MAP and to evaluate the feasibility of binary separation. Structural optimization revealed that the optimal separator configuration comprised an electrode spacing of 2 mm and a ferromagnetic body length of 5 mm with 3 mm spacing. Additionally, a numerical study also found that an auxiliary flow velocity ratio of 3 resulted in the best particle focusing effect. Furthermore, the effects of key operational parameters, including electric and magnetic field strengths and flow velocity, on particle migration were systematically investigated. The findings revealed that these factors significantly enhanced the lateral migration disparity between LFP and graphite within the separation channel, thereby enabling complete separation of LFP particles with high purity and recovery under optimized conditions. Overall, this study provides a theoretical foundation for the development of high-performance and environmentally sustainable LIBs recovery technologies. Full article
(This article belongs to the Collection Micro/Nanoscale Electrokinetics)
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23 pages, 5585 KB  
Article
NURBS Morphing Optimization of Drag and Lift in a Coupe-Class Vehicle Using Symmetry-Plane Comparison of Aerodynamic Performance
by Sohaib Guendaoui, Abdeslam El Akkad, Ahmed El Khalfi, Sorin Vlase and Marin Marin
Symmetry 2025, 17(9), 1571; https://doi.org/10.3390/sym17091571 - 19 Sep 2025
Viewed by 413
Abstract
This study presents a morphing Non-Uniform Rational B-Spline (NURBS) optimization method for enhancing sports car aerodynamics, with performance evaluation conducted in the vehicle’s symmetry plane. The morphing approach enables precise, smooth deformations of rear-end and spoiler geometries while preserving shape continuity, allowing controlled [...] Read more.
This study presents a morphing Non-Uniform Rational B-Spline (NURBS) optimization method for enhancing sports car aerodynamics, with performance evaluation conducted in the vehicle’s symmetry plane. The morphing approach enables precise, smooth deformations of rear-end and spoiler geometries while preserving shape continuity, allowing controlled aerodynamic modifications suitable for comparative analysis. Flow simulations were carried out in ANSYS Fluent 2022 using the Reynolds-Averaged Navier–Stokes (RANS) equations with the standard k-ε turbulence model, selected for its stability and accuracy in predicting boundary-layer evolution, wake behavior, and flow separation in external automotive flows. Three configurations were assessed: the baseline model, a spoiler-equipped version, and two NURBS-morphed designs. The symmetry-plane evaluation ensured bilateral balance across all variants, enabling direct comparison of drag and lift performance. The results show that the proposed morphing strategy achieved notable lift reduction and favorable drag-to-lift ratios while maintaining manufacturability. The findings demonstrate that combining NURBS-based morphing with symmetry-plane aerodynamic assessment offers an efficient, reliable framework for vehicle aerodynamic optimization, bridging geometric flexibility with robust computational evaluation. Full article
(This article belongs to the Section Mathematics)
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33 pages, 2380 KB  
Review
A Comprehensive Review of Symmetrical Multilateral Well (MLW) Applications in Cyclic Solvent Injection (CSI): Advancements, Challenges, and Future Prospects
by Shengyi Wu, Farshid Torabi and Ali Cheperli
Symmetry 2025, 17(9), 1513; https://doi.org/10.3390/sym17091513 - 11 Sep 2025
Viewed by 506
Abstract
This paper presents a comprehensive review and theoretical analysis of integrating Cyclic Solvent Injection (CSI) with multilateral well (MLW) technologies to enhance heavy oil recovery. Given that many MLW configurations inherently exhibit symmetrical geometries, CSI–MLW integration offers structural advantages for fluid distribution. CSI [...] Read more.
This paper presents a comprehensive review and theoretical analysis of integrating Cyclic Solvent Injection (CSI) with multilateral well (MLW) technologies to enhance heavy oil recovery. Given that many MLW configurations inherently exhibit symmetrical geometries, CSI–MLW integration offers structural advantages for fluid distribution. CSI offers a non-thermal mechanism for oil production through viscosity reduction, oil swelling, and foamy oil behaviour, but its application is often limited by poor sweep efficiency and non-uniform solvent distribution in conventional single-well configurations. In contrast, MLW configurations are effective in increasing reservoir contact and improving flow control but lack solvent-based enhancement mechanisms. In particular, symmetrical MLW configurations, such as dual-opposing laterals and evenly spaced fishbone laterals, can facilitate balanced solvent distribution and pressure profiles, thereby improving sweep efficiency and mitigating early breakthrough. By synthesizing experimental findings and theoretical insights from the existing literature, laboratory studies have reported that post-CHOPS CSI using a 28% C3H8–72% CO2 mixture can recover about 50% of the original oil in place after six cycles, while continuous-propagation CSI (CPCSI) has achieved up to ~85% OOIP in 1D physical models. These representative values illustrate the performance spectrum observed across different CSI operational modes, underscoring the importance of operational parameters in governing recovery outcomes. Building on this foundation, this paper synthesizes key operational parameters, including solvent composition, pressure decline rate, and well configuration, that influence CSI performance. While previous studies have extensively reviewed CSI and MLW as separate technologies, systematic analyses of their integration remain limited. This review addresses that gap by providing a structured synthesis of CSI–MLW interactions, supported by representative quantitative evidence from the literature. The potential synergy between CSI and MLW is highlighted as a promising direction to overcome current limitations. By leveraging geometric symmetry in well architecture, the integrated CSI–MLW approach offers unique opportunities for optimizing solvent utilization, enhancing recovery efficiency, and guiding future experimental and field-scale developments. Such symmetry-oriented designs are also central to the experimental framework proposed in this study, in which potential methods, such as the microfluidic visualization of different MLW configurations, spanning small-scale visualization studies, bench-scale experiments on fluid and chemical interactions, and mock field setups with pipe networks, are proposed as future avenues to further explore and validate this integrated strategy. Full article
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32 pages, 10952 KB  
Article
Study on the Gas–Liquid Two-Flow Characteristics Inside a Three-Stage Centrifugal Pump
by Xiang Zhang and Weidong Cao
Energies 2025, 18(17), 4772; https://doi.org/10.3390/en18174772 - 8 Sep 2025
Viewed by 683
Abstract
This paper takes a small three-stage centrifugal pump as the research object. Based on the RNG k-ε turbulence model and the TFM two-phase flow model, the numerical simulation of the internal gas–liquid two-phase flow was carried out, and the influence of the inlet [...] Read more.
This paper takes a small three-stage centrifugal pump as the research object. Based on the RNG k-ε turbulence model and the TFM two-phase flow model, the numerical simulation of the internal gas–liquid two-phase flow was carried out, and the influence of the inlet gas content rate of the small multistage centrifugal pump on its internal flow was analyzed. The research results show that the head and efficiency of the multistage centrifugal pump will decrease with the increase in the inlet gas content rate. As the gas content increases from 0% to 5%, the head of the multistage centrifugal pump decreases by 3% and its efficiency drops by 5%. The trend of the continuous increase in the pressure on the blade surface does not change with the increase in the inlet gas content rate. The bubble area on the surface of the first-stage impeller blade increases with the increase in the gas content rate. When the inlet gas content rate condition reaches 5%, the bubbles cover the middle section of the blade suction surface. The flow vortex structure is mainly composed of blade separation vortices and mouth ring clearance leakage vortices. The vortices inside the impeller are concentrated in the blade outlet and rim area, while the vortices inside the guide vanes are located in the flow channel area of the anti-guide vanes. With the increase in the gas content rate, the amplitude of pressure pulsation in the flow channel inside the pump decreases. Full article
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22 pages, 1043 KB  
Article
Strategies for Reducing Fat in Mayonnaise and Their Effects on Physicochemical Properties
by Cecilia Abirached, María Noel Acuña, Tatiana Carreras and Ignacio Vieitez
Foods 2025, 14(17), 3133; https://doi.org/10.3390/foods14173133 - 8 Sep 2025
Viewed by 1516
Abstract
Mayonnaise is a widely consumed food emulsion. Traditional mayonnaise contains approximately 70–80% lipids, making it a high-fat, calorie-dense food. This study aimed to develop a reduced-fat mayonnaise with physicochemical properties comparable to commercial low-fat formulations but with a lower oil content (<30%). Three [...] Read more.
Mayonnaise is a widely consumed food emulsion. Traditional mayonnaise contains approximately 70–80% lipids, making it a high-fat, calorie-dense food. This study aimed to develop a reduced-fat mayonnaise with physicochemical properties comparable to commercial low-fat formulations but with a lower oil content (<30%). Three formulations were prepared using canola oil and high-oleic sunflower oil at different concentrations (10%, 15%, and 30%), with and without the addition of synthetic antioxidants (BHA and BHT). Guar gum was used to control the viscosity of the continuous phase, adjusting its concentration between 0.75% and 1.55%. The formulations were compared with a commercial low-fat sample (MH) in terms of flow and rheological properties, color, phase separation stability, particle size, microscopy, and oxidative stability. The formulations exhibited flow behavior and Konini’s viscosity similar to MH. The 15% oil formulation (MHO-15%) had a particle size comparable to MH. Both MH and the experimental formulations exhibited a weak gel structure. To achieve the characteristic yellow color, β-carotene should be added to MHO-15%. Formulations containing canola oil and those without antioxidants showed higher susceptibility to oxidation, leading to the selection of high-oleic oil with added antioxidants. Based on these findings, a potential reduced-fat mayonnaise-type sauce could be formulated by decreasing lipid content from 30% to 15%. Full article
(This article belongs to the Special Issue Healthy Lipids for Food Processing)
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21 pages, 5927 KB  
Article
Flow Control-Based Aerodynamic Enhancement of Vertical Axis Wind Turbines for Offshore Renewable Energy Deployment
by Huahao Ou, Qiang Zhang, Chun Li, Dinghong Lu, Weipao Miao, Huanhuan Li and Zifei Xu
J. Mar. Sci. Eng. 2025, 13(9), 1674; https://doi.org/10.3390/jmse13091674 - 31 Aug 2025
Viewed by 725
Abstract
As wind energy development continues to expand toward nearshore and deep-sea regions, enhancing the aerodynamic efficiency of vertical axis wind turbines (VAWTs) in complex marine environments has become a critical challenge. To address this, a composite flow control strategy combining leading-edge suction and [...] Read more.
As wind energy development continues to expand toward nearshore and deep-sea regions, enhancing the aerodynamic efficiency of vertical axis wind turbines (VAWTs) in complex marine environments has become a critical challenge. To address this, a composite flow control strategy combining leading-edge suction and trailing-edge gurney flap is proposed. A two-dimensional unsteady numerical simulation framework is established based on CFD and the four-equation Transition SST (TSST) transition model. The key control parameters, including the suction slot position and width as well as the gurney flap height and width, are systematically optimized through orthogonal experimental design. The aerodynamic performance under single (suction or gurney flap) and composite control schemes is comprehensively evaluated. Results show that leading-edge suction effectively delays flow separation, while the gurney flap improves aerodynamic characteristics in the downwind region. Their synergistic effect significantly suppresses blade load fluctuations and enhances the wake structure, thereby improving wind energy capture. Compared to all other configurations, including suction-only and gurney flap-only blades, the composite control blade achieves the most significant increase in power coefficient across the entire tip speed ratio range, with an average improvement of 67.24%, demonstrating superior aerodynamic stability and strong potential for offshore applications. Full article
(This article belongs to the Special Issue Advanced Studies in Marine Data Analysis)
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23 pages, 4474 KB  
Article
Anthropogenic River Segmentation Case Study: Bahlui River from Romania
by Nicolae Marcoie, Ionuț Ovidiu Toma, Șerban Chihaia, Tomi Alexandrel Hrăniciuc, Daniel Toma, Cătălin Dumitrel Balan, Elena Niculina Drăgoi and Mircea-Teodor Nechita
Hydrology 2025, 12(9), 224; https://doi.org/10.3390/hydrology12090224 - 25 Aug 2025
Viewed by 1072
Abstract
This manuscript introduces a river segmentation method and explores the impact of human interventions through a long-term study of total nitrogen, total phosphorus, chemical oxygen demand, and biochemical oxygen demand. An indicator linking parameter concentrations to the river’s flow rate was used to [...] Read more.
This manuscript introduces a river segmentation method and explores the impact of human interventions through a long-term study of total nitrogen, total phosphorus, chemical oxygen demand, and biochemical oxygen demand. An indicator linking parameter concentrations to the river’s flow rate was used to assess the development of the examined parameters. The analysis spanned from 2011 to 2022, considering both seasonal and yearly variations. Normal probability plots served as statistical tools to evaluate whether the data followed normal distributions and identify outliers. The proposed segmentation divided the Bahlui River into four segments, each defined by anthropogenic stressors. It was found that, due to human activity, each river segment could be viewed as an “independent” river. This supports the idea that river segments can be analyzed separately as distinct components. The proposed segmentation approach represents an alternative approach in river water quality research, moving from traditional continuous system models to fragmented system analysis, which better reflects the reality of heavily modified river systems. The study’s findings are important for understanding how anthropogenic modifications affect river ecosystem functioning in the long term. Full article
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23 pages, 12911 KB  
Article
Research of Wind–Wave–Ship Coupled Effects on Ship Airwake and Helicopter Aerodynamic Characteristics
by Kun Zong, Luyao Qi, Yongjie Shi, Wei Han and Shan Ma
J. Mar. Sci. Eng. 2025, 13(9), 1608; https://doi.org/10.3390/jmse13091608 - 22 Aug 2025
Viewed by 497
Abstract
The oceanic wind and waves, as well as the resultant ship motions, significantly impact the ship airwake and the operation of shipborne helicopters. A numerical method coupling wind, wave, ship and helicopter is developed using multiphase flow, in which the ship motions are [...] Read more.
The oceanic wind and waves, as well as the resultant ship motions, significantly impact the ship airwake and the operation of shipborne helicopters. A numerical method coupling wind, wave, ship and helicopter is developed using multiphase flow, in which the ship motions are simulated in real time by dynamic fluid body interaction module and the helicopter rotor is modeled using the momentum source approach. By integrating the ONRT ship with the UH-60A helicopter, the unsteady aerodynamic characteristics of the ship airwake and the helicopter rotor while the ship is pitching and heaving at sea state 36 that cover moderate to extreme marine environments are studied, and the time history of rotor thrust and pitch moment at four different sea states and different hovering heights are calculated. It is shown that ship motions and deck displacements in relative sea states are highly nonlinear, making the conditions faced by helicopter landing and take-off operations vary greatly from one sea state to another. The effects of each sea state when coupling waves and ship motions varies greatly. The fluctuation of velocity components and rotor air loads in sea state 6 is up to twice that of in sea state 5, while there are less differences between the velocity fluctuation and the corresponding helicopter airloads among common sea state 3~5. The dynamic aerodynamic interference resulting from the wind–wave–ship–helicopter coupling exhibits pronounced unsteady characteristics, as the hovering rotor continuously traverses areas with varying velocities and vorticities. At the most severe sea state 6, rotor thrust fluctuations can reach up to 20%, and strong perturbations of 5~10 Hz with an amplitude of 1/3 of the total range occur due to oscillating separated shear layers, which endanger the shipborne helicopter operation and needs to be eluded. Full article
(This article belongs to the Section Ocean Engineering)
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16 pages, 1652 KB  
Article
Cell Partitioning Design for Microfluidic ATPS Devices: A Dynamic Energy Strategy and Calculation Using Chondrocytes and Model Microparticles
by Gabriel Garibaldi, Jimena Alegria, Anita Shayan, Robert Stannert, Nehal I. Abu-Lail and Gongchen Sun
Micromachines 2025, 16(8), 926; https://doi.org/10.3390/mi16080926 - 12 Aug 2025
Viewed by 731
Abstract
Sorting and isolating specific cells from heterogeneous populations are crucial for many biomedical applications, including drug discovery and medical diagnostics. Conventional methods such as Fluorescent Activated Cell Sorting (FACS) and Magnetic Activated Cell Sorting (MACS) face limitations in throughput, cost, and the ability [...] Read more.
Sorting and isolating specific cells from heterogeneous populations are crucial for many biomedical applications, including drug discovery and medical diagnostics. Conventional methods such as Fluorescent Activated Cell Sorting (FACS) and Magnetic Activated Cell Sorting (MACS) face limitations in throughput, cost, and the ability to separate subtly different cells. Cell partitioning in Aqueous Two-Phase Systems (ATPSs) offers a biocompatible and cost-effective alternative, particularly when combined with continuous-flow microfluidics. However, it remains challenging to rationally design microfluidic ATPS devices and operation to separate cells with similar origin but different phenotypes. In this paper, using a model ATPS, polyethylene glycol (PEG)—Dextran (Dex) system, and model cells, human chondrocytes (hChs), and carboxylated polystyrene (PS) microparticles, we systematically characterized the material properties affecting cell partitioning in ATPSs, such as surface energies of the solutions and cells and solution viscosities. We developed an energy balance approach between interfacial energy and viscous dissipation to estimate the interface translocation dynamic of cells partitioning into the preferred phase. Combining the experimental measurement and the energy balance model, our calculation reveals that the time required for complete cell partitioning at the ATPS interface can be exploited in microfluidic ATPS devices to separate hChs with different phenotypes (healthy and diseased). We expect our dynamic energy approach to provide a basis and a design strategy for optimizing microfluidic ATPS devices to achieve the efficient separation of phenotypically similar cell populations and further expand the potential of microfluidic cell separation. Full article
(This article belongs to the Special Issue Micro/Nanotechnology for Cell Manipulation, Detection and Analysis)
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13 pages, 4100 KB  
Proceeding Paper
Simulation and Experimental Validation of a Microfluidic Device Used for Cell Focusing and Sorting Based on an Inertial Microfluidics Technique
by Muhammad Zulfiqar, Fizzah Asif, Emad Uddin, Muhammad Irfan, Ch Abdullah, Sibghat Ullah, Danish Manshad and Hamza Mohsin
Mater. Proc. 2025, 23(1), 13; https://doi.org/10.3390/materproc2025023013 - 6 Aug 2025
Viewed by 541
Abstract
Cell separation is a major process in biomedicine and diagnostics and in the food and pharmaceutical industries. In this paper, a channel design is proposed for cell separation based on a passive cell sorting technique and sheath less flow. Initially, erythrocytes and monocytes [...] Read more.
Cell separation is a major process in biomedicine and diagnostics and in the food and pharmaceutical industries. In this paper, a channel design is proposed for cell separation based on a passive cell sorting technique and sheath less flow. Initially, erythrocytes and monocytes are injected into the designed channel, and the behavior of the particles is observed. The erythrocyte and monocyte are 8 μm and 20 μm in size, respectively. The final design is tested for different cross-sectional areas and particle sizes; 20 μm is the largest particle size that can be sorted with this design. Particles are separated due to inertial migration because the forces that focus the particles in the channels, in the form of different streams, deepen the lift force on the inertia of the moving particles. The lift force pushes the particles toward the wall, while the Dean force causes them to rotate near to the wall, stabilizing their positions. The lift and Dean forces depend on the inertia of the particles and topology of the channel, respectively. In this research, cell sorting is quantified by the distance between the two separated particles, and the trend of Δ x x versus Q is discussed. The channel throughput is also quantified in terms of the minimum and maximum allowable flow rates. Particles are best sorted by critical flow rate and Dean number. This hook-shaped design is created using polymethyl siloxane (PDMS), which is ideally suited for use in lab-on-chip (LOC) devices for continuous filtration and particle separation. The design is also experimentally tested and validated with the simulation results. Full article
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22 pages, 8896 KB  
Article
Synergistic Sequestration and Hydroxyapatite-Based Recovery of Phosphorus by the Coupling Process of CaCl2/Modified Oyster Shell and Circulating Fluidized Bed Reactor
by Xuejun Long, Nanshan Yang, Huiqi Wang, Jun Fang, Rui Wang, Zhenxing Zhong, Peng Yu, Xuelian Xu, Hao Huang, Jun Wan, Xiejuan Lu and Xiaohui Wu
Catalysts 2025, 15(8), 706; https://doi.org/10.3390/catal15080706 - 24 Jul 2025
Viewed by 726
Abstract
A novel modified oyster shell (MOS-800) was developed to enhance phosphorus sequestration and recovery from wastewater. Approximately 33.3% of phosphate was eliminated by the MOS-800, which also exhibited excellent pH regulation capabilities. In semicontinuous tests, a synergistic phosphorus separation was achieved through the [...] Read more.
A novel modified oyster shell (MOS-800) was developed to enhance phosphorus sequestration and recovery from wastewater. Approximately 33.3% of phosphate was eliminated by the MOS-800, which also exhibited excellent pH regulation capabilities. In semicontinuous tests, a synergistic phosphorus separation was achieved through the coupling process of CaCl2/MOS-800 and a circulating fluidized bed (CFB), resulting in an 86.5% phosphate separation. In continuous flow experiments, phosphorus elimination reached 98.2%. Material characterization revealed that hydroxyapatite (HAP) was the primary component of the crystallized products. Additionally, MOS-800 released 506.5–572.2 mg/g Ca2+ and 98.1 mg/g OH. A four-stage heterogeneous crystallization mechanism was proposed for the coupling process. In the first stage, Ca2+ quickly reacted with phosphate to form Ca-P ion clusters, etc. In the second stage, these clusters packed randomly to form spherical amorphous calcium phosphate (ACP). In the third stage, the ACP spheres were transformed and rearranged into sheet-like HAP crystallites, Finally, in the fourth stage, the HAP crystallites aggregated on the surface of crystal seeds, also with the addition of crystal seeds and undissolved MOS-800, potentially catalyzing the heterogeneous crystallization. These findings suggest that the CaCl2/MOS-800/CFB system is a promising technique for phosphate recovery from wastewater. Full article
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