Search Results (1,318)

The unarmored dinoflagellate Akashiwo sanguinea is a cosmopolitan harmful algal species known for forming intense blooms leading to mass mortality of fish, shellfish, and seabirds. Globally distributed populations of A. sanguinea have been classified into four ribotypes based on their characteristic sequences in LSU rRNA gene and primary geographic distributions. In this study, we compared the bacterial communities co-existing with the six strains of A. sanguinea from China and the USA (belonging to two ribotypes) using high-throughput sequencing of 16S rRNA gene amplicons. Generally, a bacterial microbiome with high diversity was found to be associated with laboratory-cultured A. sanguinea strains from different geographic origins. Based on ribotype classification, the six samples were divided into two groups (ribotype A: AsCHINA; ribotype C: AsUSA) for subsequent comparative analyses of their bacterial communities. Beta diversity analysis revealed a clear separation between the two groups, reflecting significant differences in bacterial community composition between the two ribotypes. Significantly higher abundance of nitrogen-fixing bacteria was found in the AsUSA group, suggesting that ribotype C may benefit from external nitrogen sources provided by their bacterial associates. If this also holds true in natural environments, this nitrogen-fixing partnership likely confers a competitive advantage to ribotype C in oligotrophic offshore waters, and potentially extends bloom duration when environmental nitrogen is depleted. Our study raised the possibility that different ribotypes of A. sanguinea may harbor distinct prokaryotic microbiomes in their phycospheres under stable cultivation conditions. Further comprehensive comparison among more isolates across all four ribotypes is highly necessary to validate this hypothesis. Full article

In separate traditions in the HB, the Rephaim are presented either as a living group of gigantic warriors or as shadowy figures of the underworld of Sheol. They are referred to as the rp’um in earlier Amorite Ugaritic texts, in which their role and status are much debated. This paper offers a hypothesis that, first, tracks rp’um/Rephaim antecedent traditions from the Sumerian heroic and funerary practices adopted by the Amorites to the tradition of the rp’um of the Ugaritic literature, and then tracks them on to the HB, through the Amorite connection to Mlk/Molech, in two different regional traditions found in the HB. Literary analysis and cross-cultural evidence regarding the Amorites are used to demonstrate the plausibility of this hypothesis. This paper also puts forth that: the name Hammurapi is a reference to a funerary practice and is a titular name; rpi is employed in its more basic sense of meaning “to restore/mend”; rp’um, following Good, is the passive participle, “restored/healed ones”; and Deut 2:10–11 and the biblical King Og texts do not support the Israelites having encountered living Rephaim warriors. Tracking the heroic and death-culture traditions shows that the antecedents to the biblical Rephaim were likely originally heroic-age warriors who, upon death, were cared for and were appealed to through funerary rituals for some benefit. However, these Amorite traditions were not fully understood by the Israelites when they encountered them and appropriated aspects in their representation of the Rephaim. Full article

Three-dimensional (3D) cell culture models are widely used to provide a more physiologically relevant microenvironment in which to host and study desired cell types. These models vary in complexity and cost, ranging from simple and inexpensive to highly sophisticated and costly systems. In this study, we introduce a novel translucent multi-compartmentalized stacked multilayered nanocellulose scaffold and describe its fabrication, characterization, and potential application for co-culturing multiple cell types. The scaffold consists of bacterial nanocellulose (BNC) layers separated by interlayers of a lower density of nanocellulose fibers. Using this system, we co-cultured the MDA-MB-231 cell line with two tumor-associated cell types, namely BC-CAFs and M2 macrophages, to simulate the tumor microenvironment (TME). Cells remained viable and metabolically active for up to 15 days. Confocal microscopy showed no signs of cell invasion. However, BC-CAFs and MDA-MB-231 cells were frequently observed within the same layer. The expression of breast cancer-related genes was analyzed to assess the downstream functionality of the cells. We found that the E-cadherin expression was 20% lower in cancer cells co-cultured in the multi-compartmentalized scaffold than in those cultured in 2D plates. Since E-cadherin plays a critical role in preventing the initial dissociation of epithelial cells from the primary tumor mass and is often downregulated in the tumor microenvironment in vivo, this finding suggests that our scaffold more effectively recapitulates the complexity of a tumor microenvironment. Full article

The interaction between immune regulatory cells, such as regulatory B cells (Breg) and pro-resolving macrophages (M2 macrophages), plays an important role in the restoration of immune homeostasis during inflammation. PD-L1 is one of the effector molecules that mediates the immune regulation function of M2 macrophages. The activation of PD-L1/PD-1 signaling promotes the differentiation of Breg. Previous studies have shown that Breg promoted M2 macrophage polarization and enhanced their function, but little is known about the regulatory function of M2 macrophages on Breg differentiation. This study aims to determine the effect of M2 macrophages on Breg induction and the potential mechanism in vitro. Bone-marrow-derived macrophages were isolated from wild-type (WT) mice and polarized into M2 using IL-4/IL-13. To investigate the role of PD-L1/PD-1 in M2 macrophage-induced Breg differentiation, spleen B cells were isolated from WT or PD-1 knockout (KO) mice and co-cultured with either naïve (M0) or M2 macrophages for 48 h with or without trans-well inserts. The expression of IL-10, IL-35, and TGF-β1 in B cells was evaluated by flow cytometry and immunofluorescence staining. Recombinant PD-L1 was used to stimulate activated B cells, followed by the detection of IL-35 and TGF-β1. The results show that there was no significant difference in IL-10 expression among all groups. However, IL-35 and TGF-β1 expression in B cells was significantly increased in the M2+B, but not in M0+B, compared to B cells alone. Notably, such increases were diminished when M2 and B cells were separated by trans-well inserts. IL-35 expression was not significantly changed when B cells from PD-1 KO mice were co-cultured with M2 compared to the control. However, TGF-β1 expression was significantly increased when PD-1 KO B cells were co-cultured with M2 compared to the control. IL-35 expression in activated B cells was increased upon stimulation with PD-L1. However, TGF-β1 expression in activated B cells was increased regardless of the PD-L1 availability. This study demonstrates that pro-resolving macrophage-induced IL-35⁺ but not TGF-β1⁺ regulatory B cell activation requires the PD-L1/PD-1 pathway. Full article

Constructing stable and flexible neuronal networks with multi-neurite wiring is essential for the in vitro modeling of brain function, connectivity, and neuroplasticity. However, most existing neuroengineering platforms rely on static microfabrication techniques, which limit the ability to dynamically control circuit architecture during cultivation. In this study, we developed a modifiable agarose gel-based platform that enables real-time microstructure fabrication using an infrared (IR) laser system under live-cell conditions. This approach allows for the stepwise construction of directional neurite paths, including sequential microchannel formation, cell chamber fabrication, and controlled neurite–neurite crossings. To support long-term neuronal health and network integrity in agarose microstructures, we incorporated direct glial co-culture into the system. A comparative analysis showed that co-culture significantly enhanced neuronal adhesion, neurite outgrowth, and survival over several weeks. The feeder layer configuration provided localized trophic support while maintaining a clear separation between glial and neuronal populations. Dynamic wiring experiments further confirmed the platform’s precision and compatibility. Neurites extended through newly fabricated channels and crossed pre-existing neurites without morphological damage, even when laser fabrication occurred after initial outgrowth. Time-lapse imaging showed a temporary growth cone stalling at crossing points, followed by successful elongation in all tested samples. Furthermore, the direct laser irradiation of extending neurites during microstructure modification did not visibly impair neurite elongation, suggesting minimal morphological damage under the applied conditions. However, potential effects on molecular signaling and electrophysiological function remain to be evaluated in future studies. Together, these findings establish a powerful, flexible system for constructive neuroengineering. The platform supports long-term culture, real-time modification, and multidirectional wiring, offering new opportunities for studying neural development, synaptic integration, and regeneration in vitro. Full article

The Church of Christ is unity in diversity. Around the great centers of diffusion, the rites have been gradually defined as “the liturgical, theological, spiritual and disciplinary patrimony, culture and circumstances of the history of a distinct people, by which its own manner of living the faith is manifested” (Code of Canons of the Eastern Churches can. 28 § 1). At the same time, the necessity of the existence of the sacred ministry for the celebration of the Eucharist and the Sacraments is the basis for the establishment of the hierarchy of bishoprics that are formed ontogenetically and diachronically around the primary diffusion center, recognized as the Mother Church or, starting from the IVth–Vth centuries, as the Patriarchates. The tensions between dissident factions culminated in the Ecclesiastical Schism of 1054, which separated Eastern Christianity from the Roman Church. The restoration of the unity of the Constantinopolitan Churches of Central and Eastern Europe began with the Union of Brest–Litovsk (1595–1596), which generated a process of gradual entry of the territories of the Eastern Churches into unity, in 1700 reaching Transylvania. The Greek Catholic Churches fought a pioneering struggle in asserting their own traditions in order to restore the unity of the Church. The Eastern churches that re-entered the unity of the Catholic Church faced a change of ecclesiological paradigm, being in a permanent struggle to preserve their own specificity and to affirm the unity. The signatories of the Union Acts rejected “the Uniatism” from the beginning, a fact accepted today within the theological dialogue between the Catholic Church and the Orthodox Churches, the canonical evolution and the treatises of Greek–Catholic theology being the result of a process of experimentation “from within” of unity and catholicity in the context of the modern and contemporary era. The United Churches have paved the way for the restoration of unity between East and West, being obligated to grasp different forms of canonical manifestation of unity in the absence of a Patriarchate in communion with the Church of Rome, during which they offer a reflection that fully grows through a theology of restoring the unity of the Church, benefiting today from the ecclesiological paradigm shift of Vatican II and by the conceptual tools provided by the traditions and the Code of Canons of the Eastern Churches. Full article

This study discusses how cultural interpretations of the foster parent role might shape perceptions and practices related to the temporariness of care and reunification with biological families. Employing a contrastive-comparative approach, the study compares interviews with seven Japanese foster parents, with prevailing discourses surrounding the foster parent role in Norway. The findings suggest that the Japanese foster parents implicitly recognize the temporariness of their role. In contrast, the Norwegian foster parent role has typically been associated with an emphasis on the risks associated with separation from new attachment figures, which may be perceived as detrimental to children’s well-being. We argue that this conceptualization, which frames children as inherently vulnerable, may shape the foster parent’s role as one of protection, with a primary focus on shielding the child from disruption and fostering a secure attachment. This perspective, we suggest, could have implications for the temporariness of children’s care placements and the process of reunification. The study calls for a critical examination of the values underlying current child protection practices and encourages openness to alternative perspectives, including those informed by different cultural contexts. Full article

In the field of cultural heritage (CH), non-invasive analyses, such as photogrammetry and multiband imaging (MBI), play a pivotal role as effective solutions for examining the morphology, materials, and state of preservation of an artifact. Gathering such information is particularly valuable since these data are complementary and provide a comprehensive perspective for an in-depth study of a wide variety of historically and artistically significant artifacts. Photogrammetry and MBI are commonly utilized for these purposes but typically as separate methodologies. This research seeks to address this limitation by integrating these datasets to enrich the information embedded within a 3D model, thereby facilitating the identification of areas subsequently analyzed using spectroscopic techniques. This study provides an in-depth analysis of two historically significant violins housed at the Museo del Violino in Cremona (Italy) contributing to a more comprehensive understanding of a specific category of artifacts that remains underrepresented in the existing literature. Furthermore, the technical workflow for integrating MBI data using the Physically Based Rendering (PBR) approach and Sketchfab, along with the interpretation of the resulting data, is presented. Full article

Euglena gracilis is a metabolically versatile microalga capable of thriving under photoautotrophic (light, no ethanol), mixotrophic (light with 1% v/v ethanol), and heterotrophic (dark with 1% v/v ethanol) conditions. Here, we applied untargeted LC-MS metabolomics (Agilent 1290 LC, 6545XT QTOF-MS; Agilent Technologies, Santa Clara, California, USA) to investigate its trophic-mode-dependent metabolic adaptations and assess its biotechnological potential. Metabolites were separated on a C18 column and analyzed in both positive and negative ion modes. Multivariate analyses (PCA and sPLS-DA) revealed clear and reproducible metabolic separations among growth modes (p < 0.001). Photoautotrophic cultures were enriched in phenolic acids, flavonoids, and lipid classes associated with oxidative stress protection. Mixotrophy induced a broader spectrum of upregulated metabolite classes, including saccharolipids, macrolactams, and triterpenoids, reflecting a hybrid metabolism combining photosynthesis and ethanol utilization. Heterotrophic cultures showed elevated levels of polyamines and amino acids (e.g., putrescine, proline), indicative of redox regulation and stress adaptation in dark, ethanol-rich conditions. Class-level comparisons identified distinct and shared metabolite categories, with photoautotrophy favoring antioxidant biosynthesis and mixotrophy supporting metabolic diversity. These findings provide metabolite-level insights into the extraordinary plasticity of E. gracilis and offer a framework for optimizing cultivation strategies to enhance the targeted production of high-value bioproducts. Full article

As individuals grow older, they experience notable shifts in their health conditions, social positions, and personal identity. Studies show that finding meaning in life correlates with healthier lifestyle choices and enhanced overall well-being. This sense of meaning functions as a protective factor against life’s challenges, strengthening resilience during later years. The research utilized thematic analysis within a qualitative methodology to investigate the subjective sources of meaning among elderly people living in Romanian institutional settings. The analysis revealed several interconnected themes. Religiosity and spirituality emerged as a fundamental anchor, with participants often mentioning God, Divinity, and Higher Power in their narratives. They typically conceptualized life as a sacred gift and emphasized the importance of religious practices such as prayer and church attendance as essential to their sense of purpose. The intergenerational transmission of values also proved significant, with elderly individuals finding meaning in passing down moral and spiritual principles to younger family members, viewing this as their enduring legacy. Interpersonal connections played a crucial role as well, with quality relationships with family and friends fostering a sense of belonging, while broken relationships or isolation negatively impacted well-being. Daily life objectives, including short-term goals like maintaining routines or anticipating family visits, along with preserving functional independence, provided structure and immediate purpose for many participants. The research distinguished between religiosity and spirituality as related but separate concepts: while formal religious rituals offered community-based meaning, those less engaged with organized religion discovered transcendence through personal spiritual experiences such as connecting with nature. This study highlights cultural specificities, particularly how Orthodox Christian traditions shape existential narratives among Romanian elderly, and proposes practical interventions for retirement facilities to incorporate activities focused on spiritual engagement, relationship building, and opportunities for elders to share their wisdom with younger generations. Full article

Somali refugee youth face intersecting stressors related to displacement, economic hardship, and systemic exclusion, yet their coping strategies remain understudied. This study examines the psychosocial distress, coping mechanisms, and help-seeking behaviors of Somali refugee youth in Nairobi’s urban displacement context using a mixed-methods approach. Quantitative analyses assess the prevalence of stressors, coping strategies, and perceived support, while qualitative responses provide insight into lived experiences. Findings reveal that 72% of participants reported distress linked to economic insecurity (15.9%), family separation (16.9%), or refugee-related adversities (13.2%). Religious coping (59.5%) and self-care practices (60.5%) were the most frequently used strategies, while 15.8% relied on avoidance-based methods. Help-seeking patterns showed that 76.7% of participants were willing to seek help in general, but only 40.7% did so for emotional issues, with mothers and same-sex friends being primary sources. Regression analysis (R² = 0.507, p < 0.001) showed that scope of community (β = 0.417, p = 0.001), trust in bonding social capital (β = 0.343, p = 0.012), and perceived community violence (β = 0.346, p = 0.003) were positively associated with perceived support. In contrast, help-seeking intention (β = −0.206, p = 0.049) was negatively associated with perceived support. Other variables—including religious coping, emotional coping, age, gender, and bridging trust—were not significant predictors. These findings underscore the need for community-driven mental health interventions that strengthen existing social structures while integrating culturally adapted service models. Leveraging social capital is essential for fostering sustainable, accessible, and community-based mental health support for displaced Somali youth. Full article

The aim of this work was to evaluate the impact of fortifying goat milk kefir with high-value ingredients (3% and 6% date paste, and 25% and 50% goat milk substitution with date–cheese whey), derived from the valorization of date coproducts, on its nutritional (proximate composition and mineral profile), technological (pH, acidity, viscosity, color, sugar and organic acid content), microbiological and sensory properties. Both ingredients enhanced the growth and stability of the kefir starter culture, thereby improving the probiotic potential of date-added kefir and also its nutritious quality (lower fat content and higher protein content). The mineral profile of kefir was improved only when the date paste was added. Date paste could be used as an ingredient in fortified kefir (up to 6%) without altering its flow properties because it was perfectly integrated within the milk matrix. The use of date–cheese whey as a goat milk substitution (>25%) decreased the typical kefir viscosity, inducing an excessive phase separation negatively valued by consumers. Consumers preferred the kefir with 6% date paste mainly due to its higher scores for aroma, flavor, sweetness and acidity. Full article

Vanilla is native to Mexico and has social-cultural and economic importance. It is sensory characteristics are developed during the curing process, which is associated with the region where it is carried out since the know-how of each locality is involved. In this sense, this work aimed to evaluate the influence of the curing process. Four different processes from four regions (SJA, SLP, CQ and EPM) were considered, taking into account two curing conditions. Additionally, sample control was considered. The moisture content, protein, ether extract, ash and pH were analyzed. The aromatic profile was evaluated by the RATA methodology and liking level. Except for ash content, the process influenced the other physicochemical parameters. The moisture contents of SLP and CQ samples from Period 1, as well as SLP samples from Period 2, comply with the current Mexican Standard. SJA vanilla was “slightly” accepted in both periods, surpassing the control sample. In contrast, the CQ sample was the least preferred. Thirty-five aromatic descriptors were generated. At the sensory level, a clear separation of vanillas was observed according to the type of curing. The attributes described included caramel, dry fruit, fruity, honey, maltol, rancid, sweet, tree bark, vanilla and ashes, which boosted the liking level. On the other hand, the descriptors chemical, moisture, dairy, spicy, wood and lipids had a negative effect, proving that these factors can alter the aromatic balance, giving an unpleasant smell and reducing preference. It was confirmed that the curing process influences physicochemical parameters, the aromatic profile and the liking level. However, it would be necessary to consider other variables. Full article

The International Agency for Research on Cancer has studied and classified 1045 potential substances. It is therefore important to develop rapid screening methods to identify the mutagenicity of compounds and, further on, the intensity and number of individual mutagenic substances in complex sample mixtures. The current in vitro Ames assay in the microtiter plate format (MPF) uses a pH-sensitive detection as endpoint, however, acidic substances in complex mixtures may interfere the mutagenicity result. Hence, it was transferred to the planar assay format to be more selective for complex mixture testing. The co-culture of Salmonella Typhimurium strains TA98 and TA100 with an optical density of 0.4 at 600 nm was applied on a high-performance thin-layer chromatography silica gel 60 chromatogram and on-surface incubated for 5 h, which period was limited due to zone diffusion. Various positive controls were tested, and 4-nitrochinolin-N-oxide with a limit of detection of 100 ng was established as a positive control. However, due to the shorter incubation time, no mutagenic compounds were detectable or differentiable in the tested perfumes, herbal teas, margarines, and hand creams. This does not mean that the samples are mutagen-free, but it suggests that further improvements to the bioassay are urgently needed to increase the sensitivity and selectivity of the response. Compared to conventional sum value assays, a planar Ames assay performed on the separated and adsorbed sample components advances toxicology research because mutagenic compounds are separated from interfering molecules due to the integrated separation. It thus would provide a more selective detection of mutagens in complex mixtures and allow testing of large sample volumes or concentrated samples without matrix interference. Full article

The proliferation of toxigenic fungi in food and the subsequent production of mycotoxins constitute a significant concern in the fields of public health and consumer protection. This review highlights recent strategies and emerging methods aimed at preventing fungal growth and mycotoxin contamination in food matrices as opposed to traditional approaches such as chemical fungicides, which may leave toxic residues and pose risks to human and animal health as well as the environment. The novel methodologies discussed include the use of plant-derived compounds such as essential oils, classified as Generally Recognized as Safe (GRAS), polyphenols, lactic acid bacteria, cold plasma technologies, nanoparticles (particularly metal nanoparticles such as silver or zinc nanoparticles), magnetic materials, and ionizing radiation. Among these, essential oils, polyphenols, and lactic acid bacteria offer eco-friendly and non-toxic alternatives to conventional fungicides while demonstrating strong antimicrobial and antifungal properties; essential oils and polyphenols also possess antioxidant activity. Cold plasma and ionizing radiation enable rapid, non-thermal, and chemical-free decontamination processes. Nanoparticles and magnetic materials contribute advantages such as enhanced stability, controlled release, and ease of separation. Furthermore, this review explores recent advancements in the application of artificial intelligence, particularly machine learning methods, for the identification and classification of fungal species as well as for predicting the growth of toxigenic fungi and subsequent mycotoxin production in food products and culture media. Full article