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Keywords = cytoskyrins

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2 pages, 73 KiB  
Correction
Correction: Singh, M.P., et al. Cytoskyrins and Cytosporones Produced by Cytospora sp. CR200: Taxonomy, Fermentation and Biological Activities. Mar. Drugs 2007, 5, 71-84.
by Maya P. Singh, Jeffrey E. Janso and Sean F. Brady
Mar. Drugs 2009, 7(2), 95-96; https://doi.org/10.3390/md70200095 - 14 Apr 2009
Viewed by 10833
Abstract
We found an error in Figure 1 in our paper published in the Marine Drugs [1]. The structure of Cytosporones A and B are corrected as follows: [..] Full article
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14 pages, 149 KiB  
Article
Cytoskyrins and Cytosporones Produced by Cytospora sp. CR200: Taxonomy, Fermentation and Biological Activities
by Maya P. Singh, Jeffrey E. Janso and Sean F. Brady
Mar. Drugs 2007, 5(3), 71-84; https://doi.org/10.3390/md503071 - 19 Jul 2007
Cited by 42 | Viewed by 18662 | Correction
Abstract
In screening endophytic fungi from Costa Rica for bioactivity, fungal culture CR200, isolated from a buttonwood tree, was found to contain compounds that initiate DNA damage in a test strain of E. coli (Biochemical Induction Assay, BIA) and inhibit growth of Gram-positive bacteria, [...] Read more.
In screening endophytic fungi from Costa Rica for bioactivity, fungal culture CR200, isolated from a buttonwood tree, was found to contain compounds that initiate DNA damage in a test strain of E. coli (Biochemical Induction Assay, BIA) and inhibit growth of Gram-positive bacteria, including antibiotic-resistant strains. Two new bisanthraquinones (cytoskyrins A and B) and five new related octaketides (cytosporones A-E) were isolated from fermentation broths of this fungus. Cytoskyrin A exhibited potent in-vitro antibacterial (MICs against Gram-positive bacteria, 0.03 - 0.25 μg/mL) and DNA-damaging activities (10 ng/spot), whereas cytoskyrin B was inactive in these assays. Among the cytosporones, only D and E exhibited Gram-positive activity, but they were inactive in the BIA. Mechanistically, cytoskyrin A specifically inhibited DNA synthesis in E. coli imp at its MIC; however, it also moderately inhibited protein synthesis at 2x its MIC. Cytoskyrin A exhibited poor cytotoxicity against tumor cell lines (IC50 > 5 μg/mL) compared to known antitumor agents. The nuclear ribosomal internal transcribed spacer region of CR200 was found to share highest similarity (94-96%) with Cytospora spp. Micro- and macroscopic morphological observations of the conidia and conidiomata, respectively, also suggested this fungus to be a Cytospora sp. Full article
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