Search Results (79)

Marine mammals are important ecological bio-indicators of marine ecosystems impacted by a plethora of anthropogenic and environmental threats. Genomics detects genetic variation, adaptation to environmental shifts, and susceptibility to diseases in marine mammal species. In this study, eDNA was utilized for the first time in the Pagasitikos Gulf over three consecutive years (2022–2024) in order to detect marine mammal species. Additionally, visual monitoring and eDNA results were compared to reveal the pros and cons of the two methodologies. The gulf was zoned into five different areas with respect to oceanographic features for sampling. DNA extraction was assessed by using a standard protocol of phenol–chloroform followed by PCR amplification using the 16S rRNA gene. A total of 5,209,613 highly filtered sequence reads were attributed to 108 species. Among these, Monachus monachus, Tursiops truncatus, and Ziphius cavirostris species were detected. This novel detection of Z. cavirostris in the relatively shallow waters of the Gulf of Pagasitikos raised the question of whether it was a random event or a new ecological trend. Z. cavirostris and M. monachus appeared to share the same marine areas within the gulf. In the era of the climate crisis, eDNA provides essential information on marine mammals’ ecological status, yields novel detections, and predicts behavioral changes essential to deep-diving species. Full article

Background/Objectives: Scopolamine (SCO) is widely employed as a pharmacological model of anxiety and amnesia in both rodents and zebrafish, the latter representing a valuable translational model in neuropsychopharmacology. The present study aimed to evaluate the neuroprotective and antioxidant potential of chronic administration of an ethanolic extract from Calendula officinalis flowers (CEE). Methods: Adult zebrafish (n = 10/group, both sexes) were exposed to CEE at concentrations of 1, 3, and 10 mg/L, administered daily for 22 consecutive days. After the initial 7-day pretreatment period, fish were challenged with SCO (100 μM, immersion for 30 min) followed by behavioral testing, including the Novel Tank Diving Test, Light/Dark Test, Novel Approach Test, Y-Maze, and Novel Object Recognition. Subsequently, brain homogenates were analyzed for acetylcholinesterase (AChE) activity, antioxidant enzymes (superoxide dismutase—SOD, catalase—CAT, glutathione peroxidase—GPx), reduced glutathione (GSH), protein carbonyls, and malondialdehyde (MDA). Results: Chronic CEE administration significantly attenuated scopolamine-induced anxiety-like behaviors and improved spatial memory (Y-maze) and recognition memory (NOR), as well as reduced anxiety-like behavior in the SCO-induced zebrafish model. Biochemical analyses revealed that CEE restored AChE activity, enhanced the activity of SOD, CAT, and GPx, and increased GSH levels, while concomitantly reducing protein oxidation and lipid peroxidation. The most pronounced effects were observed at 3 mg/L, which nearly normalized both behavioral and biochemical parameters. Conclusions: The CEE exerted anxiolytic and procognitive effects in zebrafish through combined cholinergic and antioxidant mechanisms. These findings highlight its translational potential as a promising candidate for the prevention and treatment of anxiety-related and cognitive disorders. Full article

This paper introduces CodeDive, a web-based programming environment with real-time behavioral tracking designed to enhance student progress assessment and provide timely support for learners, while also addressing the academic integrity challenges posed by Large Language Models (LLMs). Visibility into the student’s learning process has become essential for effective pedagogical analysis and personalized feedback, especially in the era where LLMs can generate complete solutions, making it difficult to truly assess student learning and ensure academic integrity based solely on the final outcome. CodeDive provides this process-level transparency by capturing fine-grained events, such as code edits, executions, and pauses, enabling instructors to gain actionable insights for timely student support, analyze learning trajectories, and effectively uphold academic integrity. It operates on a scalable Kubernetes-based cloud architecture, ensuring security and user isolation via containerization and SSO authentication. As a browser-accessible platform, it requires no local installation, simplifying deployment. The system produces a rich data stream of all interaction events for pedagogical analysis. In a Spring 2025 deployment in an Operating Systems course with approximately 100 students, CodeDive captured nearly 25,000 code snapshots and over 4000 execution events with a low overhead. The collected data powered an interactive dashboard visualizing each learner’s coding timeline, offering actionable insights for timely student support and a deeper understanding of their problem-solving strategies. By shifting evaluation from the final artifact to the developmental process, CodeDive offers a practical solution for comprehensively assessing student progress and verifying authentic learning in the LLM era. The successful deployment confirms that CodeDive is a stable and valuable tool for maintaining pedagogical transparency and integrity in modern classrooms. Full article

Neurodegenerative diseases (NDs) encompass a group of chronic conditions, characterized by neuronal losses in large areas of the brain, leading to cognitive and behavioral impairments. Alzheimer’s Disease (AD), the most common form of dementia, is a progressive ND, characterized by the accumulation of amyloid β and tau protein, entails cognitive decline, neuroinflammation, mitochondrial dysfunction, and blood–brain barrier impairment, with oxidative stress playing a critical role in its pathogenesis. To date, the available pharmacotherapy has shown limited efficacy, and multitarget activity of plant-derived neuroprotective bioactive compounds is currently in focus. This review synthesizes experimental evidence regarding Ocimum species with neuroprotective potential in AD, particularly Ocimum sanctum and Ocimum basilicum. These plants are rich in bioactive compounds including polyphenols, flavonoids, essential oils, and triterpenoids that synergistically scavenge reactive oxygen/nitrogen species, upregulate endogenous antioxidant enzymes (SOD, CAT, and GPx), and reduce lipid peroxidation. Furthermore, these extracts have demonstrated the ability to decrease β-amyloid accumulation and tau protein levels, key pathological features of AD. Even though additional research is required to fully assess their potential as therapeutic agents for NDs, by diving into the specific mechanisms through which they improve neurodegenerative processes, important steps can be made towards this endpoint. Full article

Background/Objectives: Perturbation in terminal N-glycan processing is a feature of congenital disorders of glycosylation and neurological disorders. Since treatment options are limited, N-glycans are plausible therapeutic targets. Here, we investigated the consequences of substituting complex/hybrid with oligomannose types of N-glycans on nervous and musculature systems, employing mgat1a and mgat1b mutant zebrafish models. Methods: CRISPR Cas9 technology was employed to engineer the mgat1a zebrafish model. The N-glycan populations in Wt AB, mgat1a−/− and mgat1b−/− zebrafish were characterized via lectin blotting. Motor and sensory functions were measured by tail-coiling and touch-evoked response assays in embryos and larvae. Swimming locomotion and anxiety-like behavior were characterized in adult Wt AB, and mutant zebrafish using motility and novel tank dive assays. Results: The mgat1a−/− model had increased oligomannosylated proteins compared to Wt AB in embryos and dissected brain, spinal cord, skeletal muscle, heart, swim bladder, and skin from adults, supporting a global knockdown of GnT-I activity. Higher levels were also observed in mgat1a−/− relative to mgat1b−/−, except in the brain. Band patterns for oligomannosylated proteins were different between all three zebrafish lines. The mgat1−/− embryos and larvae had deficient motor and sensory functions which persisted into adulthood, with a higher deficiency in mgat1b−/−. Anxiety-like behavior was decreased and increased in adult mgat1a−/− and mgat1b−/−, respectively, compared to Wt AB. Conclusions: Taken together, this study revealed that aberrant terminal N-glycan processing impacts brain, spinal and muscle control, and hence will enhance our understanding of the vital role of complex/hybrid N-glycans in nervous system health. Full article

This study dives into what drives people to use AR filters in the catering industry, focusing on the Hong Kong market. The main idea is to determine how “perceived value” shapes users’ intentions to engage with these filters. To do this, the research combines concepts from two popular models—the extended Technology Acceptance Model (TAM) and the Satisfaction–Loyalty Model (SLM)—to understand what influences perceived value. The survey data were then analyzed with Structural Equation Modeling (SEM) to see how perceived usefulness, enjoyment, satisfaction, and value connect to users’ intentions. The results showed that “perceived value” is a big deal—the main factor driving whether people want to use AR filters. Things like how useful or enjoyable the filters are and how satisfied users feel all play a role in shaping this perceived value. These findings are gold for marketing teams and AR developers, especially in the catering world. Combining TAM and the Satisfaction–Loyalty Model offers a fresh perspective on how AR technology influences consumer behavior. On top of that, it gives practical advice for businesses looking to make the most of AR filters in their marketing and customer experience strategies. Full article

Among marine organisms, baleen whale species like the humpback whale (Megaptera novaeangliae) are a case for which individual-based models are necessary to study population changes because individual trait variabilities predominate over average demographic rates to govern population dynamics. These models require quantification of individual organisms’ dynamics with respect to local conditions, which implies optimal strategy frameworks cannot be used. Instead, to quantify how individuals perform according to the environmental conditions they encounter, we formulated a model linking individual mechanical characteristics of swimming and diving with their aerobic metabolism and behavior. The model simulates the dynamics of swimming and diving for the reported range of whale sizes (1000 to 50,000 kg). Additional processes simulate foraging events including rapid accelerations and water engulfment, which modifies whale shape, weight and drag. Simulations show how the energy cost of swimming at equilibrium increases geometrically with velocity and linearly with mass. The duration and distance covered under apnea vary monotonically with mass but not with velocity; hence, there is a positive mass-dependent optimal velocity that maximizes the distance and duration of apnea. The dive limit was explored with a combination of the physiological state, mechanical force produced and distance to return to surface. This combination is imposed as an inequality constraint on the whale individual. The inequality constraint, transformed as a multi-layer perceptron, which continuously processes information about oxygen, depth and relative velocity, provides the whale individual with autonomous decision-making about whether or not to continue the dive. The results also highlight where missing metabolic information is needed to simulate the dynamics of a population of autonomous individuals at the scale of the Global Ocean. Full article

Foeniculum vulgare Mill. (Apiaceae) is an aromatic medicinal plant known for its anti-inflammatory, antispasmodic, antiseptic, carminative, diuretic, and analgesic properties. This study aimed to investigate the effects of F. vulgare essential oil (FVEO; 25, 150, and 300 μL/L) on the cognitive performance and brain oxidative stress in a scopolamine (SCOP; 100 μM)-induced zebrafish model of cognitive impairment. Additionally, the pharmacokinetic properties and bioactivity profiles of the main FVEO constituents were predicted to be used in silico tools, including SwissADME, pkCSM, PASS online, and ADMETlab 2.0. Behavioral assays, novel tank diving test (NTT), Y-maze, and novel object recognition (NOR) test, were used to evaluate anxiety-like behavior, spatial memory, and recognition memory, respectively. Biochemical assessments of acetylcholinesterase (AChE) activity and oxidative stress biomarkers were also conducted. The results demonstrated that FVEO significantly improved cognitive performance in SCOP-treated zebrafish, normalized AChE activity, and reduced oxidative stress in the brain. These findings suggest the therapeutic potential of FVEO in ameliorating memory impairment and oxidative damage associated with neurodegenerative disorders such as Alzheimer’s disease (AD). Full article

Background/Objectives: Solanum macrocarpon L. has been studied for its neuroprotective potential and memory-enhancing properties. Research suggests that bioactive compounds, including flavonoids, alkaloids, and phenolics, contribute to its cognitive benefits. These compounds may help protect against oxidative stress, neuroinflammation, and cholinergic dysfunction factors in memory impairment. This study was undertaken to investigate the effects of S. macrocarpon ethanolic leaf extract (SMEE) on the memory, anxiety-like behavior, and brain antioxidant status of scopolamine (SCOP, 100 μM)-induced amnesic zebrafish (Danio rerio) and thus to understand its possible mechanism of action. Methods: Adult zebrafish (n = 100) were divided into two cohorts (±SCOP) of five experimental groups: (I) control; (II) galantamine (GAL, 1 mg/L), serving as a positive control for both behavioral and biochemical assessments; (III–V) three groups treated with SMEE (1, 3, and 6 mg/L); (VI) scopolamine (SCOP, 100 μM); (VII) SCOP (100 μM) combined with GAL (1 mg/L); and (VIII–X) three groups treated with SCOP (100 μM) plus SMEE (1, 3, and 6 mg/L). The treatment lasted 23 days and amnesia was induced by a single dose of SCOP (100 μM) before testing. Results: The phenolic characterization from the samples was performed by using HPLC-PDA chromatography. Following HPLC analysis, an in silico pharmacokinetic evaluation was conducted using the ADMET model to investigate the pharmacological and toxicological profiles of the identified compounds. Spatial memory was evaluated through the Y-maze and novel object recognition (NOR) tests, while anxiety-like behavior was assessed using the novel tank diving test (NTT), novel approach test (NAT), and light–dark test (LDT). The zebrafish were euthanized, and homogenates of isolated brain samples were assayed for acetylcholinesterase (AChE) activity and brain antioxidant markers. The HPLC analysis revealed that the main major compounds in the extract were chlorogenic acid and rutin, both recognized for their significant antioxidant properties. Conclusions: SMEE enhanced memory by inhibiting AChE, alleviated SCOP-induced anxiety-like behavior, and significantly decreased oxidative stress markers. These findings support the potential role of SMEE in counteracting SCOP-induced cognitive and behavioral dysfunctions, related to dementia conditions. Full article

The Atlantic puffin (Fratercula arctica) is a seabird species characterized by great diving capabilities and transoceanic migratory behavior. These movements contribute to the dispersion of the species during migration, and episodes of mortality associated with migration may be a normal event in the dynamic of the Atlantic puffin populations. This study aimed to describe the anatomopathological findings of an unusual mortality event of Atlantic puffins observed during the non-breeding period along the coast of the Canary Islands. The most consistent gross finding during necropsy was generalized muscle atrophy and fat depletion. The main histological findings were centered in the urinary tract, with dilation and inflammation of the primary ureter branch and medullary cones, and intraluminal trematodes identified as Renicola sloanei based on morphology and molecular analysis. Influenza virus infection was ruled out. The postmortem investigations performed in this mortality event of Atlantic puffins indicate that the animals were severely emaciated and suffered from nephropathy. The etiopathological investigation performed in relation to this mortality event of Atlantic puffins indicates starvation associated with bad weather conditions during migratory movement as the most likely cause of the unusual mortality event. Full article

Pressure hulls are the primary pressure-bearing structures in submersibles and deep-sea space stations, which are essential for marine scientific research. Due to repeated dive cycles and extended operational periods, these hulls undergo creep deformation over time, posing risks to their structural integrity. This paper provides a comprehensive review of research on the creep behavior of pressure hulls, focusing on three key aspects: creep testing, creep constitutive models, and numerical simulation techniques. Initially, various creep testing methodologies are presented, with the experimental data serving as a foundational basis for subsequent analyses. Experimental data from creep tests form the foundation for constructing and validating constitutive models, which are critical for predicting long-term deformation. The review also explores advanced numerical simulation techniques, such as user subroutines and multiscale modeling, to analyze creep in complex pressure hull structures. Finally, based on the insights from the reviewed studies, the paper proposed potential directions for future research to address current challenges and enhance the design and maintenance of pressure hulls. Full article

Passive acoustic monitoring plays a critical role in the study of marine species, particularly in understanding the behavior of deep-diving endangered species like the Mediterranean sperm whale (Physeter macrocephalus). This paper presents an effective method for tracking sperm whales using synchronized acoustic data from four hydrophones. The tracking method estimates the location of sperm whales by measuring the time difference of arrival of detected clicks. The direction of arrival of the clicks and their reflections on the surface are then reconstructed to determine the position of the whale. The method was used to perform the first acoustic tracking study of sperm whale dives recorded in the Central Mediterranean Sea by the NEMO-O$\nu$DE cabled observatory, deployed at a depth of 2100 m in the Gulf of Catania. The data analyzed in this study were collected in August and October 2005 and include 49 five-minute recordings with the presence of sperm whale clicks. A Monte Carlo simulation revealed an estimated relative error of 2.7% in depth and 1.9% in the horizontal distance for the positioning of clicks. The algorithm successfully reconstructed 64 tracks of diving sperm whales and demonstrated its potential for monitoring within a 12 km radius. Moreover, a simultaneous tracking of a vessel and a sperm whale was performed, illustrating how the method can be used to study potential changes during dives in the presence of vessels. This method offers a reliable, non-invasive approach to studying sperm whale behavior, ecology, and interaction with anthropogenic activities. Full article

Human activities significantly contribute to the biodiversity crisis, yet wildlife tourism can promote appreciation for animals and encourage conservation. This study assessed the impact of diving and snorkeling programs at Atlantis Dubai on tourists’ emotional connections with marine life and their conservation attitudes and pro-environmental behavioral intentions. In 2023 and 2024, 346 participants were surveyed before (n = 172) and after (n = 174) their experiences diving and snorkeling with elasmobranchs. Results indicated a notable increase in positive feelings toward sharks and rays, strengthening connections and respect for these creatures. The program positively influenced attitude toward sharks, making participants more inclined to support their protection and foster a deeper connection with the ocean. Although participants’ willingness to recycle improved, overall conservation attitudes and willingness to engage in broader pro-environmental behaviors showed minor change. However, the heightened positive emotions towards sharks resulted in improved perceptions of these animals, stronger conservation attitudes, and a commitment to environmental sustainability. A deeper connection with the ocean reduced fear of sharks. This research suggests that wildlife tourism can facilitate emotional connections with marine life, potentially inspiring conservation action and highlighting the need for targeted strategies to transform these emotional bonds into lasting pro-environmental behaviors. Full article

The cholinergic pathways in the central nervous system (CNS) play a pivotal role in different cognitive functions of the brain, such as memory and learning. This review takes a dive into the pharmacological side of this important part of CNS function, taking into consideration muscarinic receptors and cholinesterase enzymes. Targeting a specific subtype of five primary muscarinic receptor subtypes (M1-M5) through agonism or antagonism may benefit patients; thus, there is a great pharmaceutical research interest. Inhibition of AChE and BChE, orthosteric or allosteric, or partial agonism of M1 mAChR are correlated with Alzheimer’s disease (AD) symptoms improvement. Agonism or antagonism on different muscarinic receptor subunits may lessen schizophrenia symptoms (especially positive allosteric modulation of M4 mAChR). Selective antagonism of M4 mAChR is a promising treatment for Parkinson’s disease and dystonia, and the adverse effects are limited compared to inhibition of all five mAChR. Additionally, selective M5 antagonism plays a role in drug independence behavior. M3 mAChR overexpression is associated with malignancies, and M3R antagonists seem to have a therapeutic potential in cancer, while M1R and M2R inhibition leads to reduction of neoangiogenesis. Depending on the type of cancer, agonism of mAChR may promote cancer cell proliferation (as M3R agonism does) or protection against further tumor development (M1R agonism). Thus, there is an intense need to discover new potent compounds with specific action on muscarinic receptor subtypes. Chemical structures, chemical modification of function groups aiming at action enhancement, reduction of adverse effects, and optimization of Drug Metabolism and Pharmacokinetics (DMPK) will be further discussed, as well as protein–ligand docking. Full article

Motorcyclists remain a disproportionately large group of vulnerable road users, with fatality rates significantly higher than that in other road groups. Additionally, fatal accidents involving motorcyclists have a more slowly decreasing trend in comparison to that of other road users, while the number of this kind of users is growing fast. For all these reasons, there is a need to understand what the key factors leading to fatal accidents are in order to identify the possible measures to minimize the accidents themselves or at least their consequences. This would permit, indeed, to positively impact the road traffic system, leading to the creation of the safest road traffic system possible, as it is the goal of the Sustainable Safety approach. The aim of this study is to dive into the mentioned problem, analyzing fatal motorcycle accidents in Slovenia over a decade, highlighting the key factors contributing to these incidents. By integrating data from four databases, the study evaluated accident trends, infrastructural elements, and rider behavior through a multi-stage analysis. Firstly, data were collected from four national, up-to-date databases that contain information about road accidents themselves, the road infrastructure, additional police data, and media descriptions. After merging this information into one comprehensive database, where each row represents all the data available for one accident, a general analysis of accidents’ trends over the considered 10-year period was developed, considering at first all fatal road accidents, then deepening it to accidents caused by a motorcyclist, and finally to single-vehicle accidents. A statistical analysis followed, aimed at identifying a statistical correlation between the accidents and the factors leading to them. The results of the first accident analysis indicated that excessive speed, incorrect driving direction, and overtaking maneuvers are the primary causes of fatal accidents, especially on non-urban roads preferred by motorcyclists. Single-vehicle accidents frequently involve collisions with roadside objects, including safety barriers and poles, underscoring the need for targeted infrastructural improvements. The following correlation analysis revealed that a total of seven factors were statistically significant: three human factors (age, gender, experience)—which were the ones with the strongest correlations—one infrastructural factor (pavement width), and three factors belonging to external conditions (accident type, cause, and location). Of these, four were positively correlated to the causer, while three, i.e., pavement width, causes, and road location, were negatively correlated. This study provides a foundation for future research on less severe accidents and proactive risk behavior analysis, aiming to improve motorcyclist safety comprehensively. Full article