Search Results (9,450)

Organoid technology has emerged as a revolutionary tool in cancer research, offering physiologically accurate, three-dimensional models that preserve the histoarchitecture, genetic stability, and phenotypic complexity of primary tumors. These self-organizing structures, derived from adult stem cells, induced pluripotent stem cells, or patient tumor biopsies, recapitulate critical aspects of tumor heterogeneity, clonal evolution, and microenvironmental interactions. Organoids serve as powerful systems for modeling tumor progression, assessing drug sensitivity and resistance, and guiding precision oncology strategies. Recent innovations have extended organoid capabilities beyond static culture systems. Integration with microfluidic organoid-on-chip platforms, high-throughput CRISPR-based functional genomics, and AI-driven phenotypic analytics has enhanced mechanistic insight and translational relevance. Co-culture systems incorporating immune, stromal, and endothelial components now permit dynamic modeling of tumor–host interactions, immunotherapeutic responses, and metastatic behavior. Comparative analyses with conventional platforms, 2D monolayers, spheroids, and patient-derived xenografts emphasize the superior fidelity and clinical potential of organoids. Despite these advances, several challenges remain, such as protocol variability, incomplete recapitulation of systemic physiology, and limitations in scalability, standardization, and regulatory alignment. Addressing these gaps with unified workflows, synthetic matrices, vascularized and innervated co-cultures, and GMP-compliant manufacturing will be crucial for clinical integration. Proactive engagement with regulatory frameworks and ethical guidelines will be pivotal to ensuring safe, responsible, and equitable clinical translation. With the convergence of bioengineering, multi-omics, and computational modeling, organoids are poised to become indispensable tools in next-generation oncology, driving mechanistic discovery, predictive diagnostics, and personalized therapy optimization. Full article

The global need for tissue and organ transplantation paved the way for plant-based scaffolds as cheap, ethical, and valuable alternatives to synthetic and animal-derived matrices for tissue regeneration. Over the years, the field has outgrown its initial scope, including the development of tissue models, platforms for drug testing and delivery, biosensors, and laboratory-grown meat. In this scoping review, we aimed to shed light on the frequency of the use of different plant matrices, the main techniques for decellularization, the functionalization methods for stimulating mammalian cell attachment, and the main results. To that purpose, we searched the keywords “decellularized” AND “scaffold” AND (“plant” OR “vegetable”) in online-available databases (Science Direct, Scopus, PubMed, and Sage Journals). From the selection and study of 71 articles, we observed a multitude of plant sources and tissues, along with a large and inhomogeneous body of protocols used for decellularization, functionalization and recellularization of plant matrices, which all led to variable results, with different extents of success (mostly in vitro). Since the field of plant-based scaffolds shows high potential for growth in the next few years, driven by emerging biotechnological applications, we conclude that future research should focus on plant sources with low economic and environmental impacts while also pursuing the standardization of the methods involved and a much deeper characterization of the scaffold performance in vivo. Full article

Service or community engaged learning has gained momentum as a strategy for developing engineering students’ professional skills while facilitating engagement with the real-world complexities of engineering problem-solving. Along with other critical scholars of engineering education, we argue that embedding social justice frameworks into engineering education, including sensibility around difference, power, and privilege, is required in order for engineering to meet the great sustainability and equity challenges of our time. This paper investigates how social justice course content and community engaged learning experiences can change engineering student attitudes toward civic engagement and social responsibility. We also explore how such content increases interest in engineering among students underrepresented in the field. Using pre-/post-survey data and focus group discussions, we conducted a quantitative and qualitative evaluation of student experience in an advanced undergraduate engineering course at a public research university that integrated social justice content with hands-on community engaged projects. Our analysis of survey results show that (1) students placed greater importance on justice-oriented civic engagement and socially responsible engineering after completing the course; (2) women and underrepresented racial/ethnic groups demonstrated greater interest in community engaged projects, and women indicated a greater interest in engineering at the end of the course than men; and (3) participation in a community engaged project also increased students’ interest in engineering, humanized problems that might have traditionally been construed as technological, and deepened the value students placed on non-technical forms of knowledge and their sense of moral and ethical responsibilities. Full article

Urban public spaces function as complex social and spatial systems, serving as fundamental elements in preserving cultural heritage and fostering democratic participation and urban stability. The dominant valuation methods depend on economic principles that prioritize financial returns and property values over social, ecological, and cultural aspects. This study examines the distinction between social value, which is based on personal experiences and social connections, and fair value, which relies on market operations and financial regulations. The research investigates how valuation practices affect spatial justice and urban governance through a conceptual framework and four empirical case studies: Syntagma Square in Athens, the metro station areas, the city of Barcelona, and waterfront redevelopment projects in Thessaloniki, London, and Mumbai. The official valuation systems we studied conceal the political elements of space while preserving social inequalities and forcing out the requirements of local communities. The proposed solution establishes an integrative, pluralist valuation system that incorporates qualitative, temporal, and ethical elements, supporting the Sustainable Development Goals (SDGs) and the New Urban Agenda. The framework transforms public space discussions from commercialized urban resources into communal civic systems, creating sustainable cities for all. Full article

Blood transfusion is a routine yet resource-intensive medical procedure. Increasing global demand, limited donor availability, and logistical and ethical constraints have driven the search for adequate blood substitutes. Hemoglobin-based oxygen carriers (HBOCs) represent a promising class of therapeutics designed to mimic the oxygen transport function of red blood cells while overcoming the challenges of storage, compatibility, and infection risk. Despite decades of research, no HBOC has yet met all criteria for widespread clinical use. This review summarizes recent advances in the design and development of hemoglobin derivatives, with a focus on their biochemical properties, safety profiles, and oxygen delivery capabilities. We also discuss current limitations and translational barriers. The successful implementation of HBOCs could significantly improve transfusion strategies, especially in emergency medicine, military applications, and resource-limited settings. Continued innovation is essential to bring safe and effective oxygen therapeutics into routine clinical practice. Full article

Background/Objectives: In Japan, the human papillomavirus (HPV) vaccine introduction process is unique, and no HPV knowledge scale with established reliability and validity currently exists. This study aimed to develop a new HPV knowledge scale and evaluate its reliability and validity for practical use. Methods: With permission from the original authors of the HPV Knowledge Scale (Jo Waller et al.), we created a Japanese version incorporating the original two subscales and adding new items. The translation process involved multiple researchers, back-translation by a professional agency, and expert review to ensure linguistic and contextual accuracy. The study was approved by the Clinical Research Ethics Review Board of the researchers’ affiliated universities and conducted between April and August 2024. Results: Reliability and validity were assessed using data from 793 parents of junior high school students, including both boys and girls. Confirmatory factor analysis showed a good model fit (Goodness-of-Fit Index [GFI] = 0.934, Adjusted GFI [AGFI] = 0.907, Comparative Fit Index [CFI] = 0.928, Root Mean Square Error of Approximation [RMSEA] = 0.063). Cronbach’s alpha ranged from 0.688 to 0.845 and item-total correlations ranged from 0.393 to 0.584. Test–retest reliability, assessed with Spearman’s rank correlation, was r = 0.791 (p < 0.001). The final scale, named the Japan HPV Knowledge Scale (J-HPV-KS), includes 17 items across five factors. Conclusions: The J-HPV-KS covers HPV-related diseases, transmission routes, natural history, and vaccines. It demonstrated sufficient reliability and validity for use in Japan and is a useful tool for assessing HPV-related knowledge among Japanese parents and guardians. Full article

Ecuador is experiencing an unprecedented escalation of violence, organized crime and public insecurity, prompting the Ministry of Education to introduce a curricular reform through Ministerial Agreement MINEDUC-MINEDUC-2024-00060-A. This reform incorporates five new subjects—Civic Education, Ethics and Integrity, Education for Sustainable Development, Socioemotional Education, Financial Education, and Education for Road Safety and Sustainable Mobility—into the national curriculum, with the explicit aim of fostering civic responsibility, ethical behavior and social cohesion. This study examines the societal and political context of the reform and analyzes its representation in the Ecuadorian press during 2024 using qualitative content analysis of publicly accessible national news articles, including reports, chronicles, interviews and press releases. The analysis focuses on the framing of the reform’s messages, the information provided and the actors featured in the coverage. Findings reveal that media narratives strongly reflect governmental discourse, portraying Civic, Ethic and Integrity Education as a moral vitamin to counteract the erosion of values and as a strategy to reinforce national identity through civic rituals. The study concludes that the reform exemplifies the integration of educational policy with sociopolitical objectives, positioning schools as central actors in long-term efforts to address societal violence and promote ethical citizenship. Full article

We here delve into the intricate and evolving concepts of brain death and consciousness, particularly at the end of life. We examine the historical and technological advancements that have influenced our understanding of death, such as mechanical ventilation and resuscitation techniques. These developments have challenged traditional definitions of death, leading to the concept of brain death, defined as the irreversible loss of all brain functions, including the brainstem. We emphasize that consciousness exists on a continuum, ranging from full alertness to deep coma and complete cessation of brain activity. It explores various disorders of consciousness, including coma, vegetative state, minimally conscious state, and locked-in syndrome, each with distinct characteristics and levels of awareness. Neuroimaging techniques, such as EEG, fMRI, and DTI, are highlighted for their crucial role in diagnosing and understanding disorders of consciousness. These techniques help to detect covert consciousness, assess brain activity, and predict recovery potential. The phenomenon of the “wave of death,” which includes a paradoxical surge in brain activity at the point of death, is also discussed. We address the challenges in defining and understanding both death and consciousness, calling for biologically grounded, ethically defensible, and culturally sensitive definitions. We advocate for standardized neuroimaging protocols, longitudinal studies, and the integration of artificial intelligence to improve diagnosis and treatment. In conclusion, the document underscores the importance of an integrated, evidence-based approach to understanding the gray zones between life and death, recognizing that consciousness and death are dynamic processes with both biological and experiential dimensions. Full article

For decades, regeneration of alveolar bone defects has depended on traditional grafting options, such as autogenous/allogenic grafts or allografts. Recently, extracted teeth was introduced as an alternative graft source. Tooth autografts are being used and have gained significant attention due to their biocompatibility, osteoconductivity, osteoinductivity, and osteogenic properties. Furthermore, tooth allografts have potential to act as natural biocomposites for oral regeneration procedures and might be advantageous options in near future. Recent advances in tooth banking, including cryopreservation, can serve to maintain bioactivity and to improve the safety, viability, and regenerative potential of teeth. They might be revolutionary in oral surgery, offering a more sustainable solution to the growing demand for bone regeneration procedures. Nevertheless, challenges such as immunogenic responses, ethical issues, and regulatory constraints persist. Ongoing research and technological innovation continue to address these problems. To date, the success rates of tooth autografts are promising, and they are regarded as a reliable option in clinical practice, with predictable outcomes in alveolar ridge preservation, sinus augmentation, periodontal regeneration, guided bone regeneration (GBR), and endodontic surgery by providing natural scaffolds for cell integration and bone remodeling. However, the scientific literature on tooth allografts is lacking. Therefore, this review aimed to comprehensively evaluate the scientific literature for comparing the properties of tooth grafts with other grafting options, in terms of processing techniques, and various clinical applications, positioning them as versatile biocomposites for the future, bridging material science and regenerative dentistry. Furthermore, possible applications of allogenic tooth grafts and overcoming current limitations are also discussed. Full article

Background: currently, there is a growing trend toward multifunctional cosmetics, which combine several active ingredients in a single product to enhance efficacy and user convenience. As ingredients may influence one another, it is important to study the behavior of mixing multiple compounds in complex formulations, especially regarding their interaction with the skin. Piceatannol, for instance, is a naturally occurring stilbene recognized for its in vitro potent antioxidant, anti-inflammatory, and anti-aging activities, making it a promising candidate for dermocosmetic use in suncare. But despite its beneficial biological activities, its cutaneous permeation remains poorly understood, particularly when delivered from complex formulations containing multiple ingredients. Objectives: in this sense, this study aimed to evaluate the in vitro skin diffusion profile of piceatannol from a passion fruit seed extract (P_ext) incorporated into a topical base (B_em) or an organic sunscreen emulsion (O_em), with or without a spilanthol-rich Acmella oleracea extract (J_ext) used as a natural permeation enhancer. Methods: due to ethical and variability issues with human and animal skins, the Strat-M™ synthetic membrane was chosen as a standardized model for the in vitro skin permeation assays. Piceatannol localization within membrane layers was examined by confocal Raman microscopy (CRM), while compound identification in donor and receptor compartments was performed via UHPLC-DAD. Results: piceatannol from B_em was detected up to 140 µm from the Strat-M™ surface and exceeded 180 µm in depth when J_ext and organic sunscreens were included in the formulation. Notably, formulations containing J_ext and those based on O_em promoted enhanced accumulation in both the stratum corneum and deeper skin layers, suggesting an improved delivery potential in lipid-rich vehicles. Conclusions: even though some instability issues were observed, piceatannol penetration into Strat-M™ from the proposed formulations was confirmed, and the results provide a foundation for further research on its topical delivery, supporting the rational development of formulations capable of harnessing its demonstrated biological properties. Full article

Recent advancements in the Internet of Things (IoT) and artificial intelligence (AI) are unlocking transformative opportunities across society. One of the most critical challenges addressed by these technologies is the ageing population, which presents mounting concerns for healthcare systems and quality of life worldwide. By supporting continuous monitoring, personal care, and data-driven decision-making, IoT and AI are shifting healthcare delivery from a reactive approach to a proactive one. This paper presents a comprehensive overview of IoT-based systems with a particular focus on the Internet of Healthcare Things (IoHT) and their integration with AI, referred to as the Artificial Intelligence of Things (AIoT). We illustrate the operating procedures of IoHT systems in detail. We highlight their applications in disease management, health promotion, and active ageing. Key enabling technologies, including cloud computing, edge computing architectures, machine learning, and smart sensors, are examined in relation to continuous health monitoring, personalized interventions, and predictive decision support. This paper also indicates potential challenges that IoHT systems face, including data privacy, ethical concerns, and technology transition and aversion, and it reviews corresponding defense mechanisms from perception, policy, and technology levels. Future research directions are discussed, including explainable AI, digital twins, metaverse applications, and multimodal sensor fusion. By integrating IoT and AI, these systems offer the potential to support more adaptive and human-centered healthcare delivery, ultimately improving treatment outcomes and supporting healthy ageing. Full article

The evolution of decentralized clinical trials, driven by advanced digital technologies, is transforming traditional clinical research. It introduces innovative methods for informed consent, remote patient monitoring, and data analysis, enhancing study efficiency, validity, and participation while reducing patient burden. Some clinical procedures can be conducted remotely, increasing trial accessibility and reducing population selection biases, particularly for cardiovascular patients. However, this also presents complex regulatory and ethical challenges. The article explores how digital platforms and emerging technologies like block chain, AI, and advanced cryptography can promote traceability, security, and transparency throughout the trial process, ensuring participant identification and documentation of each procedural step. Clear, legally compliant informed consent, often managed through electronic systems, both for research participation and data management in line with GDPR, is essential. Ethical considerations include ensuring participants understand trial information, with adaptations such as simplified language, visual aids, and multilingual support. The transnational nature of decentralized trials highlights the need for coordinated regulatory standards to overcome jurisdictional barriers and reinforce accountability. This framework promotes trust, shared responsibility, and the protection of participants rights while upholding high ethical standards in scientific research. Full article

Identifying tobacco smoke exposure in indoor environments is critical for public health, especially in vulnerable populations. In this study, we developed and validated a machine learning model to detect smoking households based on indoor air quality (IAQ) data collected using low-cost sensors. A dataset of 129 homes in Spain and Austria was analyzed, with variables including PM_2.5, PM₁, CO₂, temperature, humidity, and total VOCs. The final model, based on the XGBoost algorithm, achieved near-perfect household-level classification (100% accuracy in the test set and AUC = 0.96 in external validation). Analysis of PM_2.5 temporal profiles in representative households helped interpret model performance and highlighted cases where model predictions revealed inconsistencies in self-reported smoking status. These findings support the use of sensor-based approaches for behavioral inference and exposure assessment in residential settings. The proposed method could be extended to other indoor pollution sources and may contribute to risk communication, health-oriented interventions, and policy development, provided that ethical principles such as transparency and informed consent are upheld. Full article

The Bardo Thodol (bar do thos grol), commonly known in the West as The Tibetan Book of the Dead, has traditionally served as a spiritual manual guiding consciousness through the postmortem bardo (bar do) states toward liberation or rebirth. While prior interpretations have framed the text in ritualistic, psychological, or mystical terms, this essay proposes an original and interdisciplinary expansion: that the bardo model can serve as a framework for understanding digital identity dissolution and transformation. Drawing from Tibetan Buddhist thought, extended mind theory, digital anthropology, and cognitive science, this study reinterprets the bardo states as phenomenological thresholds that manifest not only in physical death but also in virtual liminality—moments of radical ego disintegration, avatar deconstruction, and reidentification in digital and augmented environments. By mapping the six bardos onto digital experiences, this paper presents a unique synthesis of esoteric Tibetan metaphysics and contemporary posthuman theory, offering new insights into consciousness, ethical selfhood, and the future of identity. Full article

This study examines the ethical problems of using artificial intelligence (AI) applications in higher education, focusing on activities performed by university educators. Drawing on Slovak legislation that defines educators’ responsibilities, the study classifies their activities into three categories: teaching, scientific research, and other (academic management and self-directed professional development). From standpoint of methodology, a thematic review of 42 open-access, peer-reviewed articles published between 2022 and 2025 was conducted across the Web of Science and Scopus databases. Relevant AI applications and their associated ethical issues were identified and thematically categorized. Results of this study show that AI applications are extensively used across all analysed areas of university educators’ activities. Most notably used are applications that are generative language models, editing and paraphrasing tools, learning and assessment software, management and search tools, visualizing and design tools, and analysis and management systems. Their adoption raises ethical concerns which can be thematically grouped into six categories: privacy and data protection, bias and fairness, transparency and accountability, autonomy and oversight, governance gaps, and integrity and plagiarism. The results provide universities with a structured analytical framework to assess and address ethical risks related to AI use in specific academic activities. Although the study is limited to open-access literature, it offers a conceptual foundation for future empirical research and the development of ethical, institutionally grounded AI policies in higher education. Full article