Search Results (12,238)

Danube Delta (DD), an ecologically vulnerable site, together with fish populations, which are significant food resources, are largely exposed to heavy metal contamination. This study was developed in the Babina–Cernovca sector of DD in September 2023. Zinc (Zn), and iron (Fe) were identified in water, while copper (Cu), iron (Fe), and manganese (Mn) were in sediments (mud). Proximate composition of the muscle tissues of eight fish species identified in the area was assessed. The muscle was also tested to identify heavy metals contamination. The contamination degree was assessed using bioaccumulation and bioconcentrations factors. The relation between nutritional parameters and metals was tested using bivariate and multivariate analyses. Samples were analyzed by specific laboratory tests, and data were processed using ANOVA, Spearman correlation, Principal Component Analysis (PCA), and hierarchical clustering. S. erythrophthalmus, C. gibelio, and A. alburnus have the highest metal bioaccumulation capacity, exhibiting species-specific accumulation patterns. PCA and clustering analysis reflect the influence of species and environmental factors on heavy metal accumulation in fish tissue. The study integrates the heavy metals content with nutritional parameters in fish muscular tissue, using bivariate and multivariate analysis for assessing fish vulnerability to heavy metals exposure in the Danube River. Full article

This paper presents the development of a high-resolution composite index to monitor and quantify climate-related risks across Italy. The country’s complex climatic variability, extensive coastline, and low insurance penetration highlight the urgent need for robust, locally calibrated tools to bridge the climate protection gap. Building on the methodological framework of existing actuarial climate indices, previously adapted for France and the Iberian Peninsula, the index integrates six standardised indicators capturing warm and cool temperature extremes, heavy precipitation intensity, dry spell duration, high wind frequency, and sea level change. It leverages hourly ERA5-Land reanalysis data and monthly sea level observations from tide gauges. Results show a clear upward trend in climate anomalies, with regional and seasonal differentiation. Among all components, sea level is most strongly correlated with the composite index, underscoring Italy’s vulnerability to marine-related risks. Comparative analysis with European indices confirms both the robustness and specificity of the Italian exposure profile, reinforcing the need for tailored risk metrics. The index can support innovative risk transfer mechanisms, including climate-related insurance, regulatory stress testing, and resilience planning. Combining scientific rigour with operational relevance, it offers a consistent, transparent, and policy-relevant tool for managing climate risk in Italy and contributing to harmonised European frameworks. Full article

Stealth attacks targeting industrial control systems (ICS) exploit subtle sequences of malicious actions, making them difficult to detect with conventional methods. The OPC Unified Architecture (OPC UA) protocol—now widely adopted in SCADA/ICS environments—enhances OT–IT integration but simultaneously increases the exposure of critical infrastructures to sophisticated cyberattacks. Traditional detection approaches, which rely on instantaneous traffic features and static models, neglect the sequential dimension that is essential for uncovering such gradual intrusions. To address this limitation, we propose a hybrid sequential anomaly detection pipeline that combines Markov chain modeling to capture temporal dependencies with machine learning algorithms for anomaly detection. The pipeline is further augmented by explainability through SHapley Additive exPlanations (SHAP) and causal inference using the PC algorithm. Experimental evaluation on an OPC UA dataset simulating Man-In-The-Middle (MITM) and denial-of-service (DoS) attacks demonstrates that incorporating a second-order sequential memory significantly improves detection: F1-score increases by +2.27%, precision by +2.33%, and recall by +3.02%. SHAP analysis identifies the most influential features and transitions, while the causal graph highlights deviations from the system’s normal structure under attack, thereby providing interpretable insights into the root causes of anomalies. Full article

Nowadays, fiber optic cables are a strategic issue because of their importance in telecommunications. Due to the densification of optic cables and the reduction in polymeric layer thickness, the flammability of the external sheath has to be improved. Three novel flame-retardant compositions using phosphate low-melting glasses (LMGs) as aluminum trihydrate (ATH) synergist were assessed in a polyethylene–ethylene vinyl acetate (PE-EVA) matrix. It was highlighted that LMG at a 10 wt% content reduced the peak and mean value of heat release rate (HRR), respectively, to 142 and 90 kW/m² corresponding to 52% and 42% reduction compared to ATH only. Potassium phosphate LMG was shown to perform better than sodium or zinc phosphate LMG. The improvement was assigned to the formation of an expanded mineral layer at the surface of the material during combustion that acts as a thermal shield slowing down the pyrolysis rate. The structural analysis revealed that the presence of alkaline cations in glasses led to short phosphate chains that resulted in low softening point and low-viscosity liquid. It was evidenced that under heat exposure the melted glass is likely to flow between the dehydrating ATH particles, creating a cohesive layer that expands. Additionally, interactions between ATH and LMG were also evidenced. The new crystalline species may also play a role in the cohesion of the layer. Full article

The paper presents the main features of a Web-GIS platform designed to compute real-time scenario-based seismic risk assessments at the national level. Based on the Italian experience, the platform enables DRM scientist and policymakers to readily generate seismic scenarios supporting the entire DRM cycle, including training, emergency planning, calibrating operations during response, and providing seismic risk estimates for National Disaster Risk Assessment or seismic risk reduction programs. The platform is immediately operational, relying on preloaded freeware datasets on exposure and vulnerability, and requiring only basic earthquake parameters to perform real-time analysis. At a later stage, these datasets should be replaced with more detailed and accurate national-level data. The platform generates earthquake impact assessments that include physical damage, economic and human losses, and key emergency response indicators, such as estimated displaced population, required tent camps, and EMT and USAR needs. Its key innovation lies in the ability to operate at the national scale, offering immediate usability with the possibility of further customization. As a web-based service with a user-friendly graphical interface, it is particularly suited for civil protection and DRM experts. Full article

Introduction: Epidermal growth factor receptor (EGFR) alterations exist in 15–50% of non-small cell lung cancer (NSCLC) diagnoses. Although effective therapeutics have been developed in the form of tyrosine kinase inhibitors (TKI), various mechanisms of resistance lead to treatment failure after exposure to EGFR TKI-based therapy. Of these, histologic transformation (HT) into small cell lung cancer (SCLC) represents approximately 14% of cases. Methods: Within a single institution, we retrospectively reviewed longitudinal data from both tissue and liquid biopsies of patients with histologic transformation after a diagnosis of EGFR-mutant NSCLC. We sought to further characterize the baseline and emergent genomic alterations after HT to SCLC in the context of TKI exposure, along with germline alterations that may contribute to lineage plasticity and outcomes. Results: Fifteen patients were included in our analysis. Of these, EGFR exon 19 deletions were the most frequent (n = 11, 73.3%), followed by L858R (n = 3, 20%) and L861Q (n = 1, 6.7%). The median time for transformation was 17 months (95%CI, 8.9–41.9 months). The median OS of our cohort was 51.6 months (95%CI, 26.3—NE) with a median OS post-transformation of 13.4 months. Recurrent genomic alterations included TP53, Rb1, PIK3CA, and BRAF. Germline testing revealed a pathogenic alteration in FBN1, with a recurrent variant of unknown significance (VUS) in PALLD. Conclusion: Post-transformation somatic mutation testing and germline testing at presentation revealed unique mutational profiles not previously reported in the setting of HT to SCLC. Further investigations are required to determine the optimal treatment and sequencing following HT. Full article

Modern societies are increasingly experiencing limited contact with nature, a phenomenon referred to as the “nature deficit.” The article presents a systematic review of the literature on this issue, with particular emphasis on the role of forests in mitigating its effects. The analysis, based on the Scopus and Web of Science databases, synthesizes the current state of knowledge on the consequences of nature deficit for physical, mental, and social health, while also highlighting the potential of forests as spaces supporting human well-being. The review process followed a systematic methodology, using precisely defined keyword combinations and multi-stage screening. From an initial pool of 88 publications, a critical selection process led to 11 articles that met the inclusion criteria and were analyzed in depth. The findings show that regular contact with nature reduces stress, anxiety, and ADHD symptoms, supports cognitive development, and im-proves concentration, creativity, and social skills. At the same time, there is a lack of consistent tools for clearly diagnosing nature deficit, and existing studies face significant methodological limitations (small samples, subjective measurements, lack of laboratory control). The article also identifies research gaps, particularly in the context of sustainable forest management, cultural differences, and the long-term health effects of exposure to nature. Full article

Growing global supply chain uncertainties significantly threaten China’s forage import security. The evolving characteristics of the global forage trade network directly impact the stability of China’s supply. This study constructs a directed, weighted trade network based on global forage products trade data (2000–2024). Using complex network analysis methods, it systematically analyzes the network’s topological structure and evolutionary patterns, with a focus on their impact on China’s import security. The study addresses the following questions: What evolutionary patterns does the global forage trade network exhibit in terms of its topological structure? How does the evolution of this network impact the import security of forage products in China, specifically regarding supply chain stability and risk resilience? The research findings indicate the following: (1) From 2000 to 2024, the total volume of global forage products trade increased by 48.17%, primarily driven by forage products excluding alfalfa meal and pellets, which accounted for an average of 82.04% of volume annually. Additionally, the number of participating countries grew by 21.95%. (2) The global forage products trade network follows a power–law distribution, characterized by increasing network density, a clustering coefficient that initially declines and then rises, and a shortening of the average path length. (3) The core structure of the global forage products trade network shows an evolutionary trend of diffusion from core nodes in North America, Oceania, and Asia to multiple core nodes, including those in North America, Oceania, Europe, Africa, and Asia. (4) China’s forage products trade network displays distinct phase characteristics; however, imports face significant risks from high supply chain dependency and exposure to international price fluctuations. Based on these conclusions, it is recommended that China actively expands trade relations with potential product-exporting countries in Africa, encouraging enterprises to “go global.” Additionally, China should establish a three-dimensional supply chain security system, comprising maritime, land, and storage components, to enhance risk resistance and import safety. Full article

The aim of this work was to investigate the evolutionary mechanisms of an artificial sedimentary agglomerate formed by cathodic polarization in natural seawater during its abandonment to a natural environment. Previous studies indicate that the mineralogical evolution of the material is controlled by kinetic factors and/or the local precipitation of aragonite on the brucite surface. However, the observation of the precipitation of metastable phase precipitation during the initial immersion of this material (in powder form) has suggested the possibility of a more complex mechanism. The present study builds upon previous experimental work and includes thermogravimetric analysis and infrared spectrometry. The results are analyzed using numerical experimentation to evaluate the proposed hypotheses. Findings show that the transformation mechanism is characterized by the precipitation of metastable calcium carbonate phases. Under supersaturation conditions, these hydrated phases form on the brucite surface, limiting the mineral’s contact with the solution. The subsequent transformation of these amorphous phases into aragonite further reduces brucite–solution interaction, which explains the persistence of brucite both in the residual powder after 120 h of immersion and in the consolidated material after more than 20 years of exposure to natural seawater. Full article

Coeliac disease (CD) is a chronic immune-mediated enteropathy triggered by gluten ingestion in genetically predisposed individuals carrying HLA-DQ2 or HLA-DQ8 haplotypes, characterized by small intestinal mucosal damage and systemic manifestations. This systematic review and meta-analysis aimed to compare the effectiveness of urine versus stool GIPS testing for monitoring gluten intake in coeliac patients, providing evidence-based recommendations for clinical practice. A comprehensive literature search was conducted in databases like PubMed and Embase. Studies evaluating urine or stool GIPS testing in coeliac patients were included, focusing on sensitivity, specificity, and patient adherence. The meta-analysis included six studies with a total of 572 participants. The stool GIPS testing demonstrated a pooled sensitivity of 85.1% (95% CI: 79.0–89.9%) and a specificity of 92.5% (95% CI: 88.3–95.6%), making it highly reliable for detecting gluten exposure and ruling out false positives. It also achieved an AUC of 0.9853, indicating excellent diagnostic performance. In contrast, the urine GIPS testing showed a pooled sensitivity of 55.4% (95% CI: 49.6–61.2%) and a specificity of 73.0% (95% CI: 67.4–78.1%), with an AUC of 0.7898. The heterogeneity across the studies was significant (I² > 80%), driven by variations in the population characteristics, sample handling, and testing protocols. These findings emphasize the need for standardized methodologies to enhance the reliability and comparability of results. Full article

End-of-life tyre (ELT) management is still a hot topic due to implications for sustainability and human health. This review aims to summarise the findings concerning the chemicals’ bio-accessibility/availability from the granular tyre-derived infill material used in sport surfaces. We included 14 original research articles and 5 reports (grey literature). The results included the analysis concerning volatile organic compounds (VOCs), polycyclic aromatic hydrocarbons (PAHs), phthalates, metal(loid)s and other substances. The release of some dangerous chemicals was demonstrated, even though results must be considered critically. However, the chemicals’ bioaccessibility shows a highly nuanced picture and is not, per se, sufficient to determine the risk for the exposed subjects. The lack of bioavailability and epidemiological studies analysing the exposures in real scenarios resulted in one of the main issues concerning a proper evaluation of the potential risks for human health. Full article

Eukaryotic cells respond to oxidative stress (OS), a physiological condition characterized by the accumulation of reactive oxygen species (ROS), through various protective mechanisms. The antioxidant defense system (ADS) is activated either by post-translational modifications of pre-existing proteins or through the induction of gene expression. These mechanisms protect cellular biomolecules against ROS damage. Although extensive research has been conducted in different species, there is limited information regarding the specific response of Yarrowia lipolytica to OS. This study aims to elucidate the molecular mechanisms by which Y. lipolytica responds to OS by analyzing the expression of genes encoding enzymes involved in antioxidant response, such as superoxide dismutase (Sod), catalase (Cat), and glutathione peroxidase (Gpx). The Y. lipolytica genome contains three CAT genes, one SOD gene, one copper chaperone for Sod (CCS) gene, and one GPX gene. The expression profiles of these genes were assessed in Y. lipolytica cells exposed to H₂O₂ [5 mM] over time. All genes reached their maximal expression within the first 15 min of exposure. Comparative analysis between young and aged Y. lipolytica cells subjected to OS revealed that young cells exhibited higher expression levels for all genes, with CAT3 and SOD showing the highest expression values. These findings suggest that the enzymes encoded by these genes play a crucial role in the antioxidant response of this species. To our knowledge, this is the first study demonstrating that the ADS in Y. lipolytica is regulated at the transcriptional level. Full article

This study numerically investigates the effect of hydrofoil installation on the motion responses and ride comfort of a 20 m monohull vessel operating at 10 knots in regular waves. Linear seakeeping analysis (Maxsurf Motions) and nonlinear computational fluid dynamics (CFD) simulations (STAR-CCM+) are performed to compute response-amplitude operators (RAOs); for the bare hull, the two methods agree within 5%, confirming methodological reliability. The CFD results show that hydrofoils reduce heave and pitch amplitudes by approximately 16% on average. Motion Sickness Incidence (MSI) analysis indicates negligible seasickness under Gentle Breeze conditions, even during prolonged exposure; under Moderate conditions, no seasickness is predicted within 30 min across all encounter frequencies. Although linear analysis cannot directly estimate MSI for hydrofoil-fitted cases, the observed reductions in RAOs imply improved ride comfort. Overall, these findings demonstrate that hydrofoils can enhance motion stability and passenger comfort in small, low-speed vessels, providing quantitative evidence to support design applications. Full article

This study presents the development and evaluation of an automated ultraviolet-C irradiation system for maize seed treatment, emphasizing disinfection performance, environmental control, and vision-based monitoring. The system features dual 8-watt ultraviolet-C lamps, sensors for temperature and humidity, and an air extraction unit to regulate the microclimate of the chamber. Without air extraction, radiation stabilized within one minute, with internal temperatures increasing by 5.1 °C and humidity decreasing by 13.26% over 10 min. When activated, the extractor reduced heat build-up by 1.4 °C, minimized humidity fluctuations (4.6%), and removed odors, although it also attenuated the intensity of ultraviolet-C by up to 19.59%. A 10 min ultraviolet-C treatment significantly reduced the fungal infestation in maize seeds by 23.5–26.25% under both extraction conditions. Thermal imaging confirmed localized heating on seed surfaces, which stressed the importance of temperature regulation during exposure. Notable color changes ($\Delta E>2.3$) in treated seeds suggested radiation-induced pigment degradation. Ultraviolet-C intensity mapping revealed spatial non-uniformity, with measurements limited to a central axis, indicating the need for comprehensive spatial analysis. The integrated computer vision system successfully detected seed contours and color changes under high-contrast conditions, but underperformed under low-light or uneven illumination. These limitations highlight the need for improved image processing and consistent lighting to ensure accurate monitoring. Overall, the chamber shows strong potential as a non-chemical seed disinfection tool. Future research will focus on improving radiation uniformity, assessing effects on germination and plant growth, and advancing system calibration, safety mechanisms, and remote control capabilities. Full article

Indoor sports facilities present unique challenges for air quality management due to high crowd densities and limited ventilation. This study investigated air quality in a municipal athletic facility in Komotini, Greece, focusing on concentrations of airborne particulate matter (PM_1.0, PM_2.5, PM₁₀), humidity, and temperature across spectator zones, under varying mask scenarios. Sensing devices were installed in the stands to collect high-frequency environmental data. The system, based on optical particle counters and cloud-enabled analytics, enabled real-time data capture and retrospective analysis. The main experiment investigated the impact of spectators wearing medical masks during two basketball games. The results show consistently elevated PM levels during games, often exceeding recommended international thresholds in the spectator area. Notably, the use of masks by spectators led to measurable reductions in PM_1.0 and PM_2.5 concentrations, because they seem to have limited the release of human-generated aerosols as well as the amount of movement among spectators, supporting their effectiveness in limiting fine particulate exposure in inadequately ventilated environments. Humidity emerged as a reliable indicator of occupancy and potential high-risk periods, making it a valuable parameter for real-time monitoring. The findings underscore the urgent need for improved ventilation strategies in small to medium-sized indoor sports facilities and support the deployment of low-cost sensor networks for actionable environmental health management. Full article