Search Results (1,489)

Collagen peptides derived from fish skin may be limited in food applications due to undesirable flavors. To investigate the effects of Maillard reaction modification on their physicochemical and flavor properties, collagen peptides from tilapia skin were prepared via enzymatic hydrolysis, followed by the Maillard reaction with four reducing sugars (xylose, ribose, glucose and glucosamine) through a combined procedure involving simultaneous enzyme inactivation and Maillard reaction at 100 °C. The resultant Maillard reaction products (MRPs) were characterized by analyzing free amino groups, peptide size distribution and color difference, while the reaction progression was monitored using UV absorption and fluorescence spectroscopy. The flavor profile of MRPs was analyzed through quantitative descriptive sensory evaluation and GC-MS coupled with principal component analysis. Among the four reducing sugars tested, glucosamine-induced Maillard reaction products exhibited the most pronounced physicochemical and sensory improvements. Specifically, glucosamine-MRPs showed the greatest reduction in free amino groups (0.69 μmol/L) and a notable decrease in high-molecular-weight peptides (3.31%), accompanied by an increase in low-molecular-weight fractions. Colorimetric analysis revealed a marked color change (ΔE = 31.78), and spectral analysis further confirmed intensified UV absorbance and fluorescence intensity in the glucosamine group, indicating advanced reaction progression. Sensory evaluation demonstrated a significant reduction in bitterness and enhancement of umami and saltiness. Moreover, GC-MS analysis revealed that the glucosamine-treated group exhibited the most favorable volatile profile, characterized by an increase in aromatic compounds and a substantial decrease in undesirable odorants. This study provides a theoretical basis for controlling the undesirable flavor of collagen peptides through low-extent Maillard reactions by different reducing sugars. Full article

Carob pulp flour (Ceratonia siliqua) is gaining attention as a sustainable ingredient with nutritional and functional potential. This study evaluated the partial replacement of soft wheat flour with 10% carob pulp flour in breadmaking, focusing on the role of different leavening strategies: commercial baker’s yeast (LB), a selected starter culture, Lactiplantibacillus plantarum SL31 and Saccharomyces cerevisiae SY17 (LI), and a type I sourdough (LS). Dough rheology, microbial dynamics, bread quality, acceptability, and shelf-life were assessed. Results showed that the inclusion of carob pulp flour enhances the nutritional profile while maintaining satisfactory technological performance. The leavening strategy strongly influenced the final products: breads made with commercial yeast displayed high volume and softness but were less stable during storage; LS breads achieved greater microbial stability but were limited by excessive acidity and reduced sensory acceptance; breads obtained with the selected starter culture offered the most balanced outcome, combining moderate structure with enhanced flavor and consumer preference. Overall, the findings demonstrate the feasibility of incorporating carob pulp flour into bakery products and highlight the potential of tailored starter cultures as a promising compromise between technological performance, sensory quality, and shelf-life. Future work should optimize fermentation approaches to further enhance consumer appeal and support industrial application. Full article

Growing consumer demand for healthier meat products with clean-label ingredients has increased interest in plant-based fortification strategies. The present study evaluated the effects of a multicomponent cereal supplement comprising rice (35%), buckwheat (20%), oats (20%), and corn (25%) on the physicochemical, functional, oxidative, hydrolytic, and sensory properties of meat patties. Four formulations were prepared with 0% (control), 5%, 10%, and 15% supplement inclusion. At higher inclusion levels of the cereal supplement, the patties showed reduced moisture, protein, and fat contents, while ash and carbohydrate levels increased. Conversely, ash content increased from 1.38% to 2.82%, and carbohydrates rose to 8.99%. pH remained stable (5.92–6.04), whereas aw decreased significantly at 10% (0.921) and 15% (0.889) inclusion (p < 0.05). Functional tests showed dose-dependent improvements in water-binding capacity, which increased from 65.98% in the control to 71.58% at 10% supplement, and in fat retention, which rose from 38.3% to 54.14% under the same conditions, with optimal performance observed at 10% inclusion. TBARS values in 10% and 15% formulations were 13–20% lower than control throughout storage (p < 0.05). The increase in acid number was significantly slower in supplemented patties, indicating that the cereal blend effectively inhibited lipid hydrolysis during storage. Sensory evaluation revealed maximal acceptability at 10% inclusion, with declines at 15% due to grainy texture and flavor dilution. These findings establish 10% multicomponent cereal supplementation as a promising strategy to enhance yield, shelf-life stability, and consumer appeal of meat patties without compromising processing parameters. Full article

In this study, inter-brand variations in volatile flavor compound profiles of four lager beers were systematically investigated by integrating sensory evaluation with GC-MS, GC×GC-TOF-MS, and GC-O-MS. A total of 594 volatile compounds were identified, of which 71 with odor activity values (OAV) ≥ 1 were found to contribute directly to aroma expression. Additionally, 59 compounds with taste activity values (TAV) ≥ 1 were identified and may also contribute to taste perception. Furthermore, 53 aroma-active compounds were confirmed through GC-O-MS, providing additional evidence for their sensory contribution. Partial least squares discriminant analysis (PLS-DA), correlation analysis, and flavor addition experiments revealed brand-specific differential flavor compounds. Ultimately, twenty key differential flavor compounds, encompassing esters, alcohols, aromatic compounds, acids, lactones, and others, were confirmed to contribute to fruity, floral, burnt, and sweet notes. Phenethyl alcohol, with concentrations varying from 1377.1 mg/L in QD to 3297.5 mg/L in HR, showed a more than 2.4-fold difference across brands and was strongly associated with fruity (r = 0.553) and floral (r = 0.564) aroma. These compounds acted in combination to shape distinct aroma profiles. This study provides a molecular-level basis for understanding lager beer flavor and offers practical guidance for targeted flavor modulation in brewing. Full article

The collagen jelly market is expanding amidst rising consumer health consciousness. However, the high sugar and calorie contents of traditional collagen jellies make them unsuitable for patients with diabetes or obesity. The aim of this study is to develop a low-sugar collagen jelly using fruit and vegetable powder (apple, carrot, and tomato) and to identify the optimal manufacturing conditions by evaluating physicochemical and sensory properties. Texture profile analysis (TPA), proximate composition, color analysis, total flavonoid and polyphenol content, and antioxidant activity were evaluated in jellies containing 0–10% fruit and vegetable powder. Sensory evaluation on color, flavor, taste, texture, and overall preference was performed using a seven-point Likert scale. The antioxidant capacity of the jelly increased with the addition of the fruit and vegetable powder. Among the formulations evaluated, jelly containing 8% mixed powder achieved the highest preference score, highlighting its potential for consumer acceptance. This result can be attributed to the successful integration of a low-sugar base with antioxidant-rich powders, which offers both health benefits and strong consumer appeal. Full article

The study compared the effects of conventional and vegan processing aids in the clarification of must, focusing on the phenolic and sensory characteristics of must and wine. The hypothesis was that plant protein could provide results similar to those of conventional aids containing proteins of animal origin, especially in aromatic grapes, where hyperoxidation is avoided. Conducted in 2024 in Etyek-Buda, Hungary, the initial trials subjected the Irsai Olivér grape must to gravity sedimentation with various agents. Vegan processing aids, notably the combination of pea protein and chitin-glucan, showed a gentle impact on the assimilable nitrogen content and a similar reduction in turbidity to those with animal proteins. Nitrogen flotation trials compared gelatin and the vegan alternative (a combination of pea protein and chitin–glucan) in Irsai Olivér and Chardonnay must clarification. The removal of phenolic substances was monitored using the Folin–Ciocalteu method, the acid butanol assay, and the vanillin assay. In addition, nitrogen levels were evaluated before and after the flotation experiments. The plant-based processing aid effectively improved the sensory quality of Irsai Olivér. However, the gelatin-treated Chardonnay was fresher and less bitter than the vegan option, which was less balanced and more bitter with weaker aroma and flavor. Full article

The aim of this work was to systematically evaluate the effects of Lion’s Mane Mushroom powder (LMM, 0–40%) on the physicochemical properties, structural characteristics, and flavor profile of soy protein isolate-based high-moisture meat analogues (HMMAs). Optimal incorporation of 20% LMM significantly enhanced product quality by acting as a secondary phase that inhibited lateral protein aggregation while promoting longitudinal alignment, achieving a peak fibrous degree of 1.54 with dense, ordered fibers confirmed by scanning electron microscopy. Rheological analysis showed that LMM improved viscoelasticity (G′ > G″) through β-glucan; however, excessive addition (≥30%) compromised structural integrity due to insoluble dietary fiber disrupting protein network continuity, concurrently reducing thermal stability as denaturation enthalpy (_ΔH) decreased from 1176.6 to 776.3 J/g. Flavor analysis identified 285 volatile compounds in HMMAs with 20% LMM, including 98 novel compounds, and 101 flavor metabolites were upregulated. The mushroom-characteristic compound 1-octen-3-ol exhibited a marked increase in its Relative Odor Activity Value of 18.04, intensifying mushroom notes. Furthermore, LMM polysaccharides promoted the Maillard reaction, increasing the browning index from 48.77 to 82.07, while β-glucan induced a transition in protein secondary structure from random coil to β-sheet configurations via intramolecular hydrogen bonding. In conclusion, 20% LMM incorporation synergistically improved texture, fibrous structure, and flavor complexity—particularly enhancing mushroom aroma. This research offers valuable insights and a foundation for future research for developing high-quality fungal protein-based meat analogues Full article

Artemisia selengensis Turcz. (AST), an edible-medicinal herb, contains multifunctional bioactives. This study investigated the application of AST leaf extract (ASTLE) in beer brewing, focusing on the addition stage and its impacts on fermentation dynamics, flavor profile, and functional properties. Fermentation parameters, bioactive compounds (phenolic; flavonoid), and volatiles (using HS-SPME-GC-MS) were analyzed. In vivo efficacy was assessed in high-fat diet-fed mice supplemented for 8 weeks with beer containing 10% ASTLE (post-primary fermentation), evaluating body weight change, serum lipids, and uric acid levels. It was found that adding ASTLE before primary fermentation promoted yeast activity but increased the risk of excessive diacetyl production. Adding ASTLE after primary fermentation significantly increased total phenolic and flavonoid contents. GC-MS analysis revealed that ASTLE contributed 28 additional volatile compounds, including chrysanthenone and eucalyptol, thereby enriching the beer’s flavor profile and complexity. In mice, beer with 10% ASTLE (post-primary fermentation) reduced body-weight gain, and regulated abnormal blood lipids and serum uric acid levels. Adding ASTLE after primary fermentation optimized fermentation stability, bioactive retention, flavor enhancement, and conferred benefits including body-weight regulation, lipid metabolism improvement, and uric acid control, providing a reference for developing functional beers targeting health-conscious consumers. Full article

Brewer’s spent grain enhances nutritional quality by increasing fiber and plant-based proteins and reducing the need for synthetic additives. Technologies such as extrusion and fermentation transform BSG into functional ingredients that improve texture and stability. A significant increase in antioxidant capacity was observed in enriched foods; for example, in burgers, BSG improved fiber and protein levels, while decreasing fat and calories without negatively affecting sensory acceptance. In sausages, substituting 5% of pork with BSG achieved acceptance similar to traditional formulations, and hybrid formulations with BSG maintained improved protein content while preserving texture. However, concentrations above 20% may negatively impact sensory and technological properties, by introducing undesirable flavors or altering texture. Thus, BSG is a promising source of high-value functional ingredients that contribute to the circular economy and healthier, sustainable foods. Nonetheless, more in vivo studies are needed to validate the health benefits, understand the interactions in complex matrices, assess the shelf life, and evaluate the long-term sensory perception. The “Silent Revolution” of BSG requires a multidisciplinary approach that integrates science, technology, sustainability, and effective communication with consumers. Full article

Aimed at addressing the increasingly serious problem of adulteration in Xihu Longjing, a catechin-targeted nano-enhanced visual and fluorescent dual-mode sensor array was constructed by nano porphyrins and quantum dots (QDs) for the precise analysis of Xihu Longjing from adjacent origins. This sensor array realizes the quantitative analysis of catechin enantiomers in Xihu Longjing through the selective combination of sensing units. It can accurately identify adjacent Xihu Longjing teas with different grades and storage times and can precisely detect samples with a low proportion of adulteration. At the same time, the flavor quality and antioxidant performance of Xihu Longjing tea can also be quantitatively evaluated. The dual-mode sensor array design proposed in this study provides a new idea for detecting minor differences in food authenticity and has significant application value for quality control in the tea industry. Full article

Non-alcoholic beer is increasingly popular worldwide but still faces flavor challenges compared to regular beer. These flavor-related challenges include pronounced ‘wort-like’ notes, excessive sweetness, and a lack of desirable aroma complexity. The industry is trying to improve the taste of non-alcoholic beer by trying new techniques and yeasts. A newly isolated maltose-negative brewer’s yeast (M-I) from an industrial-scale brewery collection has attracted attention due to its reduced wort-like flavor. This study aims to characterize the volatile profile of a newly isolated maltose-negative brewer’s yeast (M-I) in comparison with the well-known Saccharomycodes ludwigii. The novelty of this work lies in evaluating the aroma potential of a maltose-negative isolate newly applied in industrial brewing and its contribution to improving the flavor quality of non-alcoholic beer. An SPME-GC/MS system was used to analyze aroma compounds. According to volatile compound analysis, the M-I sample has higher amounts of esters and higher alcohol composition than the S. ludwigii beer sample. Also, it has lower amounts of Strecker aldehydes, which can give a worty off-flavor. Sensory analysis revealed that, interestingly, the control S. ludwigii sample was rated as having stronger ester notes, along with more pronounced sour and bitter characteristics, whereas the M-I sample was perceived as having a more balanced flavor, leading to a more favorable rating by the panelists. Full article

The quality of the Serbian dry fermented sausage, Sremska, was evaluated without added nitrite and pasteurized post-ripening. As an extra safety measure to eliminate Salmonella, mild heat treatments (47 °C/6.5 h or 53 °C/22.1 min) were used. The effect of starter culture on product quality was also examined. Sausages were tested at the start of production and after 30 days of storage, measuring physicochemical properties, microbiota populations, biogenic amines, lipid oxidation, and sensory qualities. The absence of nitrite did not cause significant changes in microbiota. Pasteurization lowered total viable counts and lactic acid bacteria by up to 3.5 log CFU/g, especially in sausages with starter culture. Enterobacteriaceae were fully eliminated only in pasteurized products. Pathogens like Listeria monocytogenes and Staphylococcus aureus were not detected. Moderate biogenic amine levels were found in all samples (189.4–312.2 mg/kg), with higher amounts in sausages without nitrite. Neither starter culture nor pasteurization significantly affected biogenic amine levels, although pasteurization helped limit their buildup during storage. Lipid oxidation remained low (0.14–0.25 mg/kg), with slightly higher levels in sausages with starter culture; no changes due to pasteurization or nitrite absence were observed. Sensory evaluation showed all sausages received high scores. Texture, juiciness, aroma, and flavor of sausages made with starter culture were unaffected by nitrite absence or pasteurization. Sausages without starter culture scored slightly lower without nitrite compared to those with it. Pasteurization improved texture, juiciness, aroma plus flavor, and overall acceptability of all sausages without starter culture. Overall, the study concluded that nitrite-free Sremska sausages, when pasteurized with a mild heat process, maintained good quality and enhanced safety. Full article

High grafting is a widely recognized technique for varietal renewal in aging citrus orchards. However, following high grafting, a specific ‘rootstock-interstock-scion’ combination is formed, yet the influence of interstock on scion fruit quality remains insufficiently explored. To address this gap, we conducted experiments by grafting ‘Yuanxiaochun’ ((Citrus unshiu Marcov × Citrus sinensis Osbeck) × (Citrus reticulata × Citrus paradisi)) onto three distinct interstocks (‘Yuanxiaochun’/‘Harumi’/‘Trifoliate orange’ (CJ), ‘Yuanxiaochun’/‘Ponkan’/‘Trifoliate orange’ (PG), ‘Yuanxiaochun’/‘Marumi Kumquat’/‘Trifoliate orange’ (JJ)), with ‘Yuanxiaochun’/‘Trifoliate orange’ used as a control (CK), and comprehensively evaluated their impact on fruit quality of ‘Yuanxiaochun’. Our research results show that interstock significantly increased the total soluble solids (TSSs) content of fruit. Additionally, interstocks also significantly increased the organic acid content in the fruit, particularly citric acid, which was on average 2.90 mg·g⁻¹ FW higher than CK. In terms of fruit flavor, interstocks significantly reduced the sugar/acid ratio and the sweetness/total acid ratio. However, CJ and PG showed markedly higher sweetness levels. Furthermore, interstocks led to a marked increase in both total amino acid content and flavor-active amino acid content in the fruit. Taste active values of γ-aminobutyric acid, asparagine, aspartic acid, glutamic acid, and arginine were all greater than 1, indicating a significant contribution to the fruit flavor. Moreover, interstocks increased the total flavonoid and phenol content in the fruit, thereby affecting its overall antioxidant capacity. These findings provide valuable and systematic insights for high grafting and variety renewal in citrus production. Full article

The rising popularity of ready-to-eat self-heating sauerkraut fish necessitates a meticulous production process to ensure high-quality products. This study investigated the impact of processing stages on the quality of ready-to-eat tilapia fillets. The results showed that lipid oxidation, protein degradation, pH levels, and TBA concentrations increased during processing. GC-IMS analysis revealed 56 volatile compounds in tilapia fillets, with distinct compositions at different processing stages. The flavor profiles of tilapia fillets underwent significant changes during blanching and rehydration. The levels of aldehydes and alcohols notably increased, with the blanching group exhibiting the highest concentration of aldehydes, particularly saturated linear aldehydes such as hexanal, nonanal, octanal, and benzaldehyde, which play key roles in enhancing fish flavor. Conversely, the proportion of ketones decreased following heat treatment, which is a crucial factor in mitigating undesirable fishy odors. Therefore, the optimal method for preparing ready-to-eat tilapia fillets was salting pretreatment (1.5% salt and 3% propylene glycol) at 4 °C for 1 h, blanching at 100 °C for 1 min, pre-freezing at −40 °C for 12 h, and vacuum freeze-drying at −40 °C under 20 Pa for 18 h. Finally, the dried fish fillets were vacuum-sealed for storage. Principal Component Analysis (PCA) revealed that the combined variance explained by the first two principal components post-dimensionality reduction was 95%, serving as a primary indicator of the volatile flavor profile of the fish. The dried fillets were thoroughly verified using sensory evaluation. This specific formulation garnered the highest scores in sensory evaluations, resulting in superior aroma, color, and texture attributes for the self-heating fish product. The findings of this study offer a foundational framework for developing ready-to-eat tilapia fillets and other convenient food products in the future. Full article

The objective of this research was to apply static and dynamic sensometric techniques to determine the impact of processing factors (dehydration time, frying exposure time) and storage duration on the sensory and cognitive characteristics, as well as consumer preference, of chayote chips. A total of 18 types of chips were prepared (using a combination of three frying temperatures [140, 150, 160 °C], two exposure times [5 and 10 s], and three periods of storage [0, 30, and 60 days]). A panel of 100 consumers was formed to evaluate sensory and cognitive attributes (emotions and memories) as well as overall liking, using static techniques such as Rate-All-That-Apply (RATA), Check-All-That-Apply (CATA), and a hedonic scale. Finally, the temporal dominance of sensations (TDS) dynamic technique was used to study the behavior of chips with higher levels of preference. The results of the sensory techniques indicated that the storage day factor influenced the sensory results. The samples prepared on the same day were perceived with high intensities of typical attributes of this type of food (bitter-BT, Fried-A, Sweet-A, Potato-A, Toasted-A, Chayote-A, Potato-F, Crunchy, Chayote-F, and Sweet-BT) while evoking positive emotions and memories in consumers (active, enthusiastic, free, good, good nature, happy, interested, satisfied, traditional food, family, summer, party, and mild weather). In terms of preference, consumers selected the chip samples with 0 days of storage. The TDS curves determined that the dominant attributes of the chayote chips with 0 days of storage were chayote flavor, sweet, and fried (with a dominance t = 5–20 s). Regarding the cognitive aspect, these chayote chips evoke positive dominant emotions (good, satisfied, and happy from t = 8–20 s) as well as dominant positive memories of childhood (t = 9–20 s), traditional food (t = 11–20 s), and friendship (t = 11–20 s). Full article