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Keywords = green globular bodies (GGBs)

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19 pages, 3537 KB  
Article
Efficient In Vitro Plantlet Regeneration from Stolon Explants and Genetic Stability Assessment Using ISSR Markers in the Ornamental Fern Hypolepis punctata
by Xinyuan Wang, Xuetong Yan, Keyuan Zheng, Hui Shen, Jianguo Cao, Qiang Zhou and Mulan Zhu
Plants 2025, 14(16), 2569; https://doi.org/10.3390/plants14162569 - 18 Aug 2025
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Abstract
Hypolepis punctata, an aromatic fern with insect-resistant and ornamental potential. Up to date, no studies have reported its micropropagation, particularly using vegetative organs as explants. The optimized stolon sterilization (81.11%) employed 75% ethanol (30 s) and 15% sodium hypochlorite (12 min). The [...] Read more.
Hypolepis punctata, an aromatic fern with insect-resistant and ornamental potential. Up to date, no studies have reported its micropropagation, particularly using vegetative organs as explants. The optimized stolon sterilization (81.11%) employed 75% ethanol (30 s) and 15% sodium hypochlorite (12 min). The optimal conditions for GGB induction (75.56%) and proliferation (8.46 mm) were achieved using Murashige and Skoog (MS) medium + 2.0 mg/L 6-benzylaminopurine (BA) + 0.2 mg/L 1-naphthaleneacetic acid (NAA). The optimal plant growth regulator (PGR) formula for sporophyte regeneration was 0.5 mg/L BA + 0.1 mg/L NAA + 2 g/L activated charcoal (AC), achieving a 98.89% induction rate and 49.19 buds per explant. The 1/4 MS medium had the greatest promoting effect on biomass accumulation and leaf expansion. Optimal shoot elongation (97.78% success, 4.83 cm) was achieved in 1/4 MS + 0.5 mg/L BA + 0.1 mg/L NAA + 2 g/L AC, and optimized rooting (92.22%) was achieved using 1/4 MS + 0.5 mg/L indole-3-butyric acid (IBA) + 0.1 mg/L NAA + 2 g/L AC, producing 25.27 roots per plantlet. Crucially, ISSR analysis confirmed the genetic stability of all regenerants. This optimized protocol establishes a scalable micropropagation system, enhancing both commercial cultivation and genetic improvement potential in Hypolepis punctata. Full article
(This article belongs to the Topic Genetic Breeding and Biotechnology of Garden Plants)
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11 pages, 2594 KB  
Article
Optimizing Green Globular Body Induction for Micropropagation of Microsorum pteropus ‘Windeløv’
by Wirawan Suwannamali, Kuang-Teng Wang, Chia-Chen Su, Phunsin Kantha, Yuh Tzean and Tsung-Meng Wu
Horticulturae 2024, 10(7), 673; https://doi.org/10.3390/horticulturae10070673 - 25 Jun 2024
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Abstract
Microsorum pteropus ‘Windeløv’ is a water fern from Asia commonly used in aquarium landscapes. This study aimed to develop a mass production method through the induction of green globular bodies (GGBs) from leaf explants of M. pteropus. Surface sterilization was performed [...] Read more.
Microsorum pteropus ‘Windeløv’ is a water fern from Asia commonly used in aquarium landscapes. This study aimed to develop a mass production method through the induction of green globular bodies (GGBs) from leaf explants of M. pteropus. Surface sterilization was performed on adventitious buds on the fronds of M. pteropus ‘Windeløv’ as explants. The results showed that the optimal explant disinfection condition was 1% sodium hypochlorite solution for 20 min, achieving a successful rate of 87.5 ± 9.6%. The most effective GGB induction was achieved with MS medium supplemented with 5.0 mg/L of NAA (1-Naphthaleneaceticacid) and 0.5 mg/L of 6-BA (6-benzylaminopurine), producing the highest number and size of GGBs, with a mean value of 4.0 ± 0.8 GGBs per explant and a diameter of 2.45 ± 0.61 mm after 8 weeks of culture. The regeneration of multiple sporophytes from the GGBs was only observed under hormone-free MS medium, with a mean value of 34.2 ± 7.3 sporophytes per GGB after 6 weeks of culture. Subsequently, rootless and separated sporophytes could be directly transplanted into the aquarium with a 100% survival rate. Full article
(This article belongs to the Section Floriculture, Nursery and Landscape, and Turf)
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