Search Results (1,162)

Marine plastic pollution has become a critical transboundary environmental issue, particularly affecting coastal regions with insufficient waste management infrastructure. This study applies a modified Lagrangian hydrodynamic model, TrackMPD v.1, to simulate the movement and accumulation of macroplastics in the West Africa Coastal Area. The research investigates three case studies: (1) the Liberia–Gulf of Guinea region, (2) the Mauritania–Gulf of Guinea coastal stretch, (3) the Cape Verde, Mauritania, and Senegal regions. Using both forward and backward simulations, macroplastics’ trajectories were tracked to identify key sources and accumulation hotspots. The findings highlight the cross-border nature of marine litter, with plastic debris transported far from its source due to ocean currents. The Gulf of Guinea emerges as a major accumulation zone, heavily impacted by plastic pollution originating from West African rivers. Interesting connections were found between velocities and directions of the plastic debris and some of the characteristics of the West African Monson climatic system (WAM) that dominates the area. Backward modelling reveals that macroplastics beached in Cape Verde largely originate from the Arguin Basin (Mauritania), an area influenced by fishing activities and offshore oil and gas operations. Results are visualized through point tracking, density, and beaching maps, providing insights into plastic distribution and accumulation patterns. The study underscores the need for regional cooperation and integrated monitoring approaches, including remote sensing and in situ surveys, to enhance mitigation strategies. Future work will explore 3D simulations, incorporating degradation processes, biofouling, and sinking dynamics to improve the representation of plastic behaviour in marine environments. This research is conducted within the Global Development Assistance (GDA) Agile Information Development (AID) Marine Environment and Blue Economy initiative, funded by the European Space Agency (ESA) in collaboration with the Asian. Development Bank and the World Bank. The outcomes provide actionable insights for policymakers, researchers, and environmental managers aiming to combat marine plastic pollution and safeguard marine biodiversity. Full article

Guinea pigs (Cavia porcellus) are bred as livestock in South American countries from the Andean region, including Ecuador. Despite their importance for the local rural economy, no public health management guidelines have ever been implemented for guinea pig farming. Moreover, several reports have shown the carriage of diverse zoonotic pathogens in livestock guinea pigs, including respiratory and enteric pathogens, or Toxoplasma gondii. Leptospirosis, a zoonotic disease caused by bacteria from Leptospira genus, is endemic in Ecuador and has multiple animal reservoirs, with rodents considered to be the main vector for transmission to humans. However, the role of livestock guinea pigs in the epidemiology of leptospirosis is poorly understood. In this study, the prevalence of antibodies against Leptospira in guinea pigs from Ecuador was analyzed with the Microaglutiantion Test (MAT). Moreover, the shedding of Leptospira by backyard livestock guinea pigs was also addressed, analyzing fecal and urine samples by qPCR for lipL32, secY and rrs genes. From the 118 guinea pigs analyzed by MAT, 61.9% were positive for 19 different pathogenic serovars, with Icterohaemorrhagiae, Pomona, Castellonis and Bataviae being the most prevalent ones. From the 231 urine samples and 225 fecal samples collected, 27.7% and 78.7% were positive for Leptospira by qPCR, respectively. Our results highlight the role of livestock guinea pigs as a reservoir for leptospirosis. Moreover, this study underscores the zoonotic threat associated with the shedding of Leptospira within households in rural communities where guinea pigs are bred as livestock. Animal and public health guidelines from a One Health perspective must be implemented to prevent leptospirosis transmission from guinea pigs in Ecuador and other countries in the Andean Region where the breeding of backyard livestock guinea pigs is common. Full article

Optical mapping, nanotechnology-based multielectrode arrays and automated patch-clamp allow transmembrane voltage mapping with high spatial resolution, as well as L-type calcium and inward rectifier currents measurements using native mammalian cardiomyocytes. However, these methods are limited to in vitro and ex vivo experiments, while magnetocardiography (MCG) might offer a novel approach for non-invasive preclinical safety assessments of new drugs in intact and even conscious rodents by reconstructing the ventricular action potential waveform (rVAPw) from MCG signals. Objective: This study aims to assess the feasibility of rVAPw reconstruction from MCG signals in Guinea pigs (GPs) and validate the results by comparison with simultaneously recorded epicardial ventricular monophasic action potentials (eVMAP). Methods: Unshielded MCG (uMCG) data of 18 GPs, investigated anaesthetized and awake at ages of 5, 14, and 26 months using a 36-channel DC-SQUID system, were analyzed to calculate rVAPw from MCG’s current arrow map. Results: Successful rVAPw reconstruction from averaged MCG showed good alignment with eVMAP waveforms. However, some rVAPw displayed incomplete or distorted repolarization at sites with lower MCG amplitude. Conclusions: 300-s uMCG averaging allowed rVAPw reconstruction in intact GPs. Occasionally distorted rVAPw suggests the need for dedicated MCG devices development, with higher density of optimized vector sensors, and modelling tailored for small animal hearts. Full article

The Príncipe Island is in the Gulf of Guinea, along the Cameroon Volcanic Line (West Africa). It is known worldwide for its biodiversity for whose conservation there is strong national and international consensus. But its geodiversity has played and continues to play a very important role in creating this unique habitat in sub-Saharan Africa, and represents an important asset in promoting local tourism, with a focus on the geological history of this remote island. The purpose of this study is to provide a comprehensive synthesis that is both up-to-date and inclusive, incorporating all the fundamental information and references on the geodiversity and the geological heritage of the Príncipe Island. The geological heritage of Príncipe Island is represented by ten geological heritage sites of different types (volcanological, geomorphological, sedimentological, and stratigraphical), and five viewpoints, whose geoheritage contents make it possible to support a narrative about the geological history of the Cameroon Volcanic Line that is of major relevance for the development of geoeducational resources and potentially appealing to fostering regional geotourism. Príncipe geological heritage sites were inventoried and assessed using the same qualitative methodology previously applied to the geological heritage of São Tomé Island, therefore making it possible to complete the country’s national inventory of geological sites, as well as to enrich the representation of African geoheritage among the world’s list of geological heritage sites. Full article

Doxorubicin (Dox) is not a first-line treatment for melanoma due to limited antitumor efficacy and dose-dependent cardiotoxicity. However, sublethal doses may trigger adaptive cellular responses that influence tumor progression and systemic toxicity. Small extracellular vesicles (EVs) are key mediators of intercellular communication and can carry bioactive molecules that modulate both the tumor microenvironment and distant tissues. This study investigates how sublethal Dox exposure alters EV biogenesis and cargo in A375 melanoma cells and explores the potential implications for cardiovascular function. We treated human A375 melanoma cells with 10 nM dox for 96 h. EVs were isolated using differential ultracentrifugation and size exclusion chromatography. Vesicle characterization included Immunocytochemistry for CD63, CD81, CD9, Rab7 and TSG101, scanning electron microscopy (SEM) Nanoparticle Tracking Analysis (NTA), and Western blotting for CD81 and CytC. We analyzed cytokine content using cytokine membrane arrays. Guinea pig cardiomyocytes were exposed to the isolated vesicles, and mitochondrial activity was evaluated using the MTT assay. Statistical analysis included t-tests, ANOVA, Cohen’s d, and R² and η². Dox exposure significantly increased EV production (13.6-fold; p = 0.000014) and shifted vesicle size distribution. CD81 expression was significantly upregulated (p = 0.0083), and SEM (microscopy) confirmed enhanced vesiculation. EVs from treated cells were enriched in TGF-β (p = 0.0134), VEGF, CXCL1, CXCL12, CCL5, IL-3, IL-4, IL-10, Galectin-3, and KITLG. Cardiomyocytes exposed to these vesicles showed a 2.3-fold reduction in mitochondrial activity (p = 0.0021), an effect absent when vesicles were removed. Bioinformatic analysis linked EV cargo to pathways involved in cardiac hypertrophy, inflammation, and fibrosis. As conclusion, sublethal Doxorubicin reprograms melanoma-derived EVs by enhancing their production and enriching their cargo with profibrotic and immunomodulatory mediators. These vesicles may contribute to tumor progression and cardiovascular physiopathology, suggesting that targeting EVs could improve therapeutic outcomes in cancer and cardiovascular disease. Full article

The genus Sclerodermus Latreille (Hymenoptera: Bethylidae) comprises over 80 species of ectoparasitoids of insect pests in forests, agricultural environments, and stored products with a cosmopolitan distribution. Despite its growing significance in biological control, behavioral ecology, and public health, the taxonomy of the genus remains poorly resolved. This is largely due to morphological reduction and simplification among species, outdated or incomplete original descriptions, and limited access to type material. A particularly problematic case is Sclerodermus cereicollis Kieffer, originally described from two geographically disjunct populations: Giglio Island (Italy, Palaearctic) and Annobón Island (Equatorial Guinea, Afrotropical). The syntype series includes morphologically divergent specimens, casting doubt on their conspecificity. In this study, we redescribe S. cereicollis based on both the original syntypes and newly collected material from Italy. A lectotype is designated to stabilize the nomenclature, and we provide the first molecular data for the species to assess genetic cohesion among populations. Comparative morphological and molecular analyses reveal that the Afrotropical syntypes represent a distinct, previously undescribed species. Accordingly, we describe Sclerodermus annobonensis Masini, Colombo & Azevedo sp. nov., designating a holotype. This study refines species boundaries within Sclerodermus and highlights the value of integrative taxonomy, combining historical and contemporary data, in resolving persistent systematic ambiguities in morphologically conservative taxa. Full article

The neuropeptide’s multifaceted involvement in various components of neural homeostasis impacts pain and behavioral regulation. One of the highly potent neuropeptides is dermorphin, extracted from the skin of the Amazon frog (Phyllomedusa sauvagei). The unique feature of dermorphin is the D-Ala residue in its sequence, which has inspired researchers to search for dermorphin analogs for use as pharmaceuticals. The primary objective of this study is to synthesize several new linear and cyclic dermorphin analogs and evaluate them as potential non-invasive analgesics. By exploring our method for converting linear peptides into 2,5-diketopiperazine(2,5-DKP) derivatives, which stabilize peptide structures, we synthesize several new dermorphin linear peptides and chimeric cyclopeptidomimetics. These compounds were tested in vitro and in vivo to determine their biological activities and potential applicability as pharmaceuticals. For the evaluation of in vitro opioid activity, the “Guinea Pig Ileum” (GPI) test was used. D2 showed the highest activity, and cyclopeptides D3 and D4 showed high activity. We can assume that dermorphin analogues D2, D3, and D4 are potent agonists of µ-type opioid receptors and have high opioid activity. However, this needs to be verified using molecular modeling methods in further research. The analgesic effects of dermorphins have been evaluated in the “Hot-Plate” and “Tail-Flick” tests. In rats, D2 dermorphin analogues demonstrated dose-dependent analgesic effect in the “Water Tail-Flick” test after intranasal administration. A smaller dose of 0.5 µg/kg resulted in 40% analgesia and a short-term state of stupor. The maximum long-lasting analgesia was observed at a dose of 1.0 µg/kg, which induced complete stupor. The analgesic effect of peptide D2 after intraperitoneal administration at a 5.0 mg/kg dose was over 50%. The “Open-Field” test demonstrated a dose-dependent (15, 50, 150 μg/kg) peptide D2 suppression effect on behavioural reactions in rats following intranasal administration. A new modification of linear peptides, combined with a 2,5-DKP scaffold (D3 and D4), proved promising for oral use based on the results of analgesic effect evaluation in mice following intragastric administration. Full article

A novel skin test for an in vivo assessment of SARS-CoV-2-specific T-cell immunity was developed using CoronaDermPS, a multiepitope recombinant polypeptide encompassing MHC II–binding CD4+ T-cell epitopes of the SARS-CoV-2 structural proteins (S, E, M) and full length nucleocapsid (N). In silico epitope prediction and modeling guided antigen design, which was expressed in Escherichia coli, was purified (>95% purity) and formulated for intradermal administration. Preclinical evaluation in guinea pigs, mice, and rhesus macaques demonstrated a robust delayed type hypersensitivity (DTH) response at optimal doses (10–75 µg), with no acute or chronic toxicity, mutagenicity, or adverse effects on reproductive organs. An integrated clinical analysis included 374 volunteers stratified by vaccination status (EpiVacCorona, Gam-COVID-Vac, CoviVac) prior to COVID-19 infection (Wuhan/Alpha, Delta, Omicron variants), and SARS-CoV-2–naïve controls. Safety assessments across phase I–II trials recorded 477 adverse events, of which >88% were mild and self-limiting; no severe or anaphylactic reactions occurred. DTH responses were measured at 24 h, 72 h, and 144 h post-injection by papule and hyperemia measurements. Overall, 282/374 participants (75.4%) exhibited a positive skin test. Receiver operating characteristic analysis yielded an overall AUC of 0.825 (95% CI: 0.726–0.924), sensitivity 79.5% (95% CI: 75.1–83.3%), and specificity 85.5% (95% CI: 81.8–88.7%), with comparable diagnostic accuracy across vaccine, and variant subgroups (AUC range 0.782–0.870). CoronaDerm-PS–based skin testing offers a simple, reproducible, and low-cost method for qualitative evaluation of T-cell–mediated immunity to SARS-CoV-2, independent of specialized laboratory equipment (Eurasian Patent No. 047119). Its high safety profile and consistent performance across diverse cohorts support its utility for mass screening and monitoring of cellular immune protection following infection or vaccination. Full article

This study evaluates growth and income distribution targets needed to alleviate poverty and eradicate hunger, and assesses strategies to achieve these goals in rural areas in South Africa. Most development policy studies concentrate on growth, inequality, and poverty reduction, while explicit SDG-related applications receive less attention, especially in Africa. To fill this gap, we apply a framework that combines a recursive dynamic CGE model with a microeconomic simulation model in a top-down and bottom-up fashion. We explore two scenarios: a business-as-usual simulation and an agricultural growth simulation that tests investment, export enhancement, productivity improvements, and social assistance extension. The agriculture policy includes targeted social assistance. Halving poverty and eradicating extreme hunger requires 2.7% annual economic growth and 3.6% agricultural growth from 2018 to 2030. In the business-as-usual scenario, poverty is expected to rise from 55.2% in 2015 to 56.1% by 2030, with 24% still below the food poverty line. The agricultural growth scenario can advance hunger and poverty goals if individual consumption increases by 2.6% annually. Achieving SDG targets for hunger and poverty demands interventions beyond agricultural policy. South Africa can achieve its hunger and poverty SDG goals through a combination of agricultural investments, social assistance, and labour policies. Full article

This study assesses the impact of heatwaves on hospital admissions in the Matam region of Senegal by combining climatic indices with machine learning methods. Using daily maximum temperature (TMAX) and heat index (HI), heatwave events were identified from 2017 to 2022. Hospital data from Ourossogui Regional Hospital were analyzed, and three predictive models, Random Forest (RF), Extreme Gradient Boosting (XGB), and Generalized Additive Models (GAMs), were compared. A bootstrapping approach with 1000 iterations was used to evaluate model robustness. The findings reveal a significant delayed effect of heatwaves, with increased hospitalizations occurring three to five days after the event. RF outperformed the other models with R² values ranging from 0.51 to 0.72. These findings highlight the need to enhance heatwave monitoring and promote the integration of impact-based climate forecasting into health early warning systems, particularly to protect vulnerable groups such as the elderly, children, and outdoor workers. Full article

The tick Amblyomma sculptum is the vector of the bacterium Rickettsia rickettsii (which causes Brazilian Spotted Fever in humans). It can be found in domestic and wild animals, as well as humans. The objective of this work was to evaluate the efficacy of baits containing fluazuron in the control of A. sculptum in guinea pigs that were artificially infested. The work was conducted in two studies: descriptive pharmacokinetics and efficacy. Descriptive pharmacokinetics was assessed after administering one fluazuron-containing bait per animal (10 mg/kg). To determine the pharmacokinetic profile, blood samples were collected over several days. For the efficacy test, the animals were divided into four groups: a control group (untreated) and three treated groups that received a single dose of 10 mg/kg on days −21 (G1), −14 (G2) and −7 (G3). All animals were infested with A. sculptum larvae on day 0 and recovered on days +4, +5, +6, and +7. Fluazuron plasma concentrations increased rapidly, indicating rapid absorption, and decreased slowly. In all treated groups, engorged larvae with morphological and behavioral changes were observed. Although fluazuron did not show acaricidal efficacy, it was able to interfere with tick molting. Fluazuron was effective in controlling the A. sculptum tick in guinea pigs. Full article

Drought is a complex and impactful natural hazard, with sometimes catastrophic impacts on small or subsistence agriculture and water security. In Pacific Island countries, there lacks an agreed approach for monitoring agricultural drought hazard with satellite-derived remote sensing data. This study addresses this gap through a framework for agricultural drought monitoring in the Pacific using freely available space-based observations. Applying World Meteorological Organization’s (WMO) recommendations and a set of objective selection criteria, three remotely sensed drought indicators were chosen and combined using fuzzy logic to form a composite drought hazard index: the Standardised Precipitation Index, Soil Water Index, and Normalised Difference Vegetation Index. Each indicator represents a subsequential flow-on effect of drought on agriculture. The index classes geographic areas as low, medium, high, or very high levels of drought hazard. To test the drought hazard index, two case studies for drought in the western Pacific, Papua New Guinea (PNG), and Vanuatu, are assessed for the 2015–2016 El Niño-related drought. Findings showed that at the height of the drought in October 2015, 58% of PNG and 72% of Vanuatu showed very high drought hazard, compared to 6% and 40%, respectively, at the beginning of the drought. The hazard levels calculated were consistent with conditions observed and events that were reported during the emergency drought period. Application of this framework to operational drought monitoring will promote adaptive capacity and improve resilience to future droughts for Pacific communities. Full article

Background: Meniere’s disease (MD) is a rare inner ear disorder characterized by endolymphatic hydrops and symptoms such as vertigo and hearing loss, with no curative treatment currently available. XuanYunNing tablets (XYN) have been clinically used to treat MD, but their molecular mechanisms remain unclear. Objective: This study aimed to systematically evaluate the pharmacological effects of XYN in a guinea pig model of MD and to elucidate the underlying molecular mechanisms of both MD pathogenesis and XYN intervention through integrated multi-omics analyses, including transcriptomics, proteomics, and bioinformatics. Methods: A guinea pig model of endolymphatic hydrops was induced by intraperitoneal injection of desmopressin acetate (dDAVP). Pharmacodynamic efficacy was evaluated via behavioral scoring and histopathological analysis. The differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) modulated by XYN treatment were identified using high-throughput transcriptomic and proteomic sequencing. These data were integrated through multi-omics bioinformatic analysis. Key molecular targets and signaling pathways were further validated using RT-qPCR and Western blotting. Results: Pharmacological evaluations showed that guinea pigs in the model group exhibited a 26% increase in endolymphatic hydrops area, while high-dose XYN treatment reduced this area by 19% and significantly improved functional parameters, including overall physiological condition (e.g., weight and general appearance), auricular reflexes to low-, medium-, and high-frequency sound stimuli, nystagmus, and the righting reflex. High-throughput sequencing combined with integrative omics analysis identified 513 potential molecular targets of XYN. Subsequent network and module analyses pinpointed the JAK-STAT signaling pathway as the central axis. Mendelian randomization (MR) analysis further supported a causal relationship between MD and metabolic, immune, and inflammatory traits, reinforcing the central role of JAK-STAT signaling in both MD progression and XYN-mediated intervention. Mechanistic studies confirmed that XYN downregulated IFNG, IFNGR1, JAK1, p-STAT3/STAT3, and AOX at both mRNA and protein levels, thereby inhibiting aberrant JAK-STAT pathway activation in MD model animals. In addition, a total of 125 chemical constituents were identified in XYN by UHPLC-MS analysis. ZBTB20 and other molecules were identified as potential blood-based biomarkers for MD. Conclusions: This study reveals that XYN alleviates MD symptoms by disrupting a pathological cycle driven by JAK-STAT signaling, inflammation, and metabolic dysfunction. These findings support the clinical potential of XYN in the treatment of Meniere’s disease and may inform the development of novel therapeutic strategies. Full article

The Gulf of Guinea is undergoing accelerated sea-level rise (SLR), with localized rates surpassing 10 mm yr⁻¹, more than double the global mean. Integrating GRACE/FO ocean mass data, reanalysis products, and machine learning, we identify a regime shift in the regional sea-level budget post-2015. Over 60% of observed SLR near major riverine outlets stems from ocean mass increase, driven primarily by intensified terrestrial hydrological discharge, marking a transition from steric to barystatic and manometric dominance. This shift coincides with enhanced monsoonal precipitation, wind-forced equatorial wave adjustments, and Atlantic–Pacific climate coupling. Piecewise regression reveals a significant 2015 breakpoint, with mean coastal SLR rates increasing from 2.93 ± 0.1 to 5.4 ± 0.25 mm yr⁻¹ between 1993 and 2014, and 2015 and 2023. GRACE data indicate extreme mass accumulation (>10 mm yr⁻¹) along the eastern Gulf coast, tied to elevated river discharge and estuarine retention. Dynamical analysis reveals the reorganization of wind field intensification, which modifies Rossby wave dispersion and amplifies zonal water mass convergence. Random forest modeling attributes 16% of extreme SLR variance to terrestrial runoff (comparable to wind stress at 19%), underscoring underestimated land–ocean interactions. Current climate models underrepresent manometric contributions by 20–45%, introducing critical projection biases for high-runoff regions. The societal implications are severe, with >400 km² of urban land in Lagos and Abidjan vulnerable to inundation by 2050. These findings reveal a hybrid steric–manometric regime in the Gulf of Guinea, challenging existing paradigms and suggesting analogous dynamics may operate across tropical margins. This calls for urgent model recalibration and tailored regional adaptation strategies. Full article

The adoption of wireless medical technologies in hospital environments is often limited by cellular coverage issues, especially in indoor areas with complex structures. This study presents a detailed radio spectrum measurement campaign conducted at the Cova da Beira University Hospital Center (CHUCB), using the NARDA SRM-3006 and R&S^®TSME6 equipment. The signal strength and quality of 5G NR, LTE, UMTS, and NB-IoT technologies were evaluated. Critical coverage gaps were identified, particularly at points 17, 19, and 21. Results revealed that operators MEO and NOS dominate coverage, with MEO providing better 5G NR coverage and NOS excelling in LTE signal quality. Based on the results, the localized installation of femtocells is proposed to improve coverage in these areas. The approach was designed to be scalable and replicable, with a planned application at Cumura Hospital (Guinea-Bissau), reinforcing the applicability of the solution in contexts with limited infrastructure. This work provides both technical and clinical contributions to achieving ubiquitous cellular coverage in healthcare settings. Full article