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31 pages, 3217 KB  
Article
Accelerating Electric 3-Wheeler Adoption Through Experiential Trials: Insights and Learnings from Amritsar, Punjab
by Seshadri Raghavan, Shubhi Vaid and Ritika Sen
World Electr. Veh. J. 2025, 16(10), 554; https://doi.org/10.3390/wevj16100554 (registering DOI) - 28 Sep 2025
Abstract
Three-wheelers (3Ws—autos or auto-rickshaws) occupy a unique yet salient and substantive position within the context of India’s urban mobility. They provide critical first-and-last-mile connectivity, fill public transit coverage gaps, boost local and urban agglomeration economies, and are a major income source for millions. [...] Read more.
Three-wheelers (3Ws—autos or auto-rickshaws) occupy a unique yet salient and substantive position within the context of India’s urban mobility. They provide critical first-and-last-mile connectivity, fill public transit coverage gaps, boost local and urban agglomeration economies, and are a major income source for millions. Their value and utility are especially pronounced in rapidly emerging Tier-II cities such as Amritsar. The city’s 7500-strong diesel 3W (d3W) fleet is the backbone of its transportation network but also contributes to air pollution. Though Amritsar’s favorable policies to transition the d3W fleet to electric (e3W) have reduced purchase costs by 40–60%, barriers remain. This study investigates the influence of the e3W user experience through a first-of-a-kind three-day pilot trial for ~300 d3W drivers. By leveraging a pre- and post-intervention framework combining surveys and trip diaries, this study evaluated how direct exposure influences adoption intentions, perceptions, and the social dynamics underpinning decision-making. In total, ~6% of participants switched to e3Ws following the trial, and there was a 20% drop in “don’t know” answers regarding charging duration and range. The results show non-random and meaningful shifts in attitudes, a greater awareness of range and charging times, improved views on charging convenience and vehicle safety, and air quality benefits. Full article
(This article belongs to the Section Marketing, Promotion and Socio Economics)
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18 pages, 5902 KB  
Review
Heart at Hand: The Role of Point-of-Care Cardiac Ultrasound in Internal Medicine
by Piero Tarantini, Francesco Cei, Fabiola Longhi, Aldo Fici, Salvatore Tupputi, Gino Solitro, Lucia Colavolpe, Stefania Marengo and Nicola Mumoli
J. Cardiovasc. Dev. Dis. 2025, 12(10), 379; https://doi.org/10.3390/jcdd12100379 - 24 Sep 2025
Viewed by 34
Abstract
Bedside echocardiography stands as a cornerstone diagnostic tool in internal medicine, offering rapid, real-time evaluation of cardiac structure and function across a wide spectrum of acute and chronic conditions. Its application, particularly when combined with lung and inferior vena cava (IVC) ultrasound, significantly [...] Read more.
Bedside echocardiography stands as a cornerstone diagnostic tool in internal medicine, offering rapid, real-time evaluation of cardiac structure and function across a wide spectrum of acute and chronic conditions. Its application, particularly when combined with lung and inferior vena cava (IVC) ultrasound, significantly enhances diagnostic accuracy for fluid balance assessment, dyspnea, and hypotensive states, guiding timely therapeutic decisions. Focused cardiac ultrasound (FoCUS) enables internists to assess left ventricular function, right atrial pressure, valvular abnormalities, and pericardial effusion, facilitating differentiation between cardiac and non-cardiac causes of symptoms such as dyspnea, chest pain, and hemodynamic instability. While operator-dependent, echocardiography can be effectively integrated into internal medicine practice through structured training programs that combine theoretical knowledge with supervised hands-on experience. This integration enhances clinical decision-making, optimizes patient management, and reduces the need for immediate specialist consultation. Widespread adoption of focused ultrasound techniques in internal medicine wards promises not only improved patient outcomes but also more efficient utilization of healthcare resources. Continued education and institutional support are fundamental to embedding echocardiography into routine care, ensuring internists are equipped to leverage this powerful bedside modality. This narrative review aims to underscore the transformative impact of bedside echocardiography in internal medicine, demonstrating its capacity, when combined with lung and IVC ultrasound, to optimize diagnostic pathways and treatment decisions across diverse acute and chronic settings. Full article
(This article belongs to the Section Imaging)
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14 pages, 219 KB  
Article
Integration of Information and Communication Technology in Curriculum Practices: The Case of Preservice Accounting Teachers
by Lineo Mphatsoane-Sesoane, Loyiso Currell Jita and Molaodi Tshelane
Computers 2025, 14(9), 398; https://doi.org/10.3390/computers14090398 - 19 Sep 2025
Viewed by 201
Abstract
This empirical paper explores South African preservice accounting teachers’ perceptions of ICT integration in secondary schools’ accounting curriculum practices. Since 2020, curriculum practices have been characterised by disruptions to traditional teaching and learning methods, including those brought on by the COVID-19 pandemic. Curriculum [...] Read more.
This empirical paper explores South African preservice accounting teachers’ perceptions of ICT integration in secondary schools’ accounting curriculum practices. Since 2020, curriculum practices have been characterised by disruptions to traditional teaching and learning methods, including those brought on by the COVID-19 pandemic. Curriculum practices in accounting were not unnoticed. These sparked discussions about pedagogical changes, academic continuity, and the future of accounting curriculum practices. The theoretical framework used to guide the research process is connectivism. The theory is about forming connections between people and technology and teaching and learning in a connectivist learning environment. Connectivism promotes a lifelong learning perspective by training teachers and students to adapt to a fast-changing environment. An interpretive paradigm underpins this qualitative research paper. The data were collected from semi-structured interviews with five preservice accounting teachers about how they navigated pedagogy while switching to digital curriculum practices. Thematic analysis was used. The findings revealed that preservice accounting teachers faced challenges in ICT integration during school-based training, including limited resources, inadequate infrastructure, and insufficient hands-on training. While ICT tools enhanced learner engagement, barriers such as low digital skills and a lack of technical support hindered effective use. Participants highlighted a disconnect between theoretical training and classroom practice, prompting self-directed learning to bridge skill gaps. The study underscores the need for teacher education programs to provide practical, immersive ICT training to equip future educators for technology-driven classrooms. Full article
13 pages, 582 KB  
Review
Exploring Manual Interventions for Infantile Colic: A Scoping Review of the Evidence
by Roberto Tedeschi and Federica Giorgi
Children 2025, 12(9), 1246; https://doi.org/10.3390/children12091246 - 17 Sep 2025
Viewed by 309
Abstract
Background: Infantile colic affects up to 40% of otherwise healthy infants and can severely distress caregivers. Manual therapies are increasingly employed as non-pharmacological options, yet their effectiveness and safety remain uncertain. Methods: A scoping review was conducted in accordance with Joanna Briggs Institute [...] Read more.
Background: Infantile colic affects up to 40% of otherwise healthy infants and can severely distress caregivers. Manual therapies are increasingly employed as non-pharmacological options, yet their effectiveness and safety remain uncertain. Methods: A scoping review was conducted in accordance with Joanna Briggs Institute methodology and reported following PRISMA-ScR. Five databases (MEDLINE, CENTRAL, Scopus, PEDro, Web of Science) were searched from December 2024 to May 2025 without restrictions at the search stage; however, only English-language randomised controlled trials published from 2012 onwards were included at the eligibility stage to ensure consistency and focus on the most recent body of evidence. Randomised controlled trials (RCTs) evaluating hands-on interventions for infants ≤ 6 months with colic were eligible. Two reviewers independently screened records, charted data, and grouped outcomes narratively. Results: Seven RCTs investigated abdominal massage, paediatric Tuina, craniosacral therapy, chiropractic manipulation, osteopathic light touch, reflexology, and acupressure. Five trials reported statistically or clinically significant reductions in daily crying (0.6–6.6 h) compared with usual care or sham. Three studies also documented meaningful gains in sleep duration (1.1–2.8 h). Parent-reported satisfaction improved in most interventions. No serious adverse events were recorded, although safety monitoring was inconsistently reported. Substantial heterogeneity in diagnostic criteria, outcome measures, and intervention dose precluded meta-analysis. Conclusions: Low-force manual therapies may offer modest short-term relief for colicky infants and improve parental experience, with an apparently favourable safety profile. However, methodological variability and small sample sizes limit certainty. Standardised protocols, objective outcome measures, and robust adverse-event surveillance are priorities for future research. Full article
(This article belongs to the Section Pediatric Nursing)
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24 pages, 1328 KB  
Review
Challenges and Drivers for the Adoption of Improved Solar Drying Technologies in Mango Farming: A Case Study of Smallholder Farmers in Mozambique
by Paula Viola Salvador, Selorm Kugbega, Claudia Lazarte, Lucas Tivana and Federico Gómez Galindo
Sustainability 2025, 17(18), 8325; https://doi.org/10.3390/su17188325 - 17 Sep 2025
Viewed by 377
Abstract
Mango production plays a vital role in rural livelihoods in Mozambique, yet post-harvest losses remain high, ranging from 25% to over 50%, due to inadequate preservation methods. Improved solar drying technologies offer a sustainable solution by extending shelf life and enhancing product quality. [...] Read more.
Mango production plays a vital role in rural livelihoods in Mozambique, yet post-harvest losses remain high, ranging from 25% to over 50%, due to inadequate preservation methods. Improved solar drying technologies offer a sustainable solution by extending shelf life and enhancing product quality. However, their adoption among smallholder mango farmers remains limited. This study investigates the key barriers and drivers influencing the uptake of these technologies in three districts of Inhambane Province, through a combination of literature review and semi-structured interviews. Major barriers include limited technical knowledge, high upfront costs, poor market access, and limited institutional support. Field data show that over 80% of farmers are unaware of improved fruit drying techniques, and fewer than 5% have received any training. While the literature emphasizes environmental sustainability and policy support, field interviews highlight a stronger interest in practical enablers, such as affordable systems, hands-on training, and income-generating potential. The study calls for a multisectoral approach, involving financial support, capacity-building, and strengthened extension services to promote adoption. Scaling solar drying technologies can significantly reduce post-harvest losses and strengthen the mango value chain, contributing to food security and rural development in Mozambique. Full article
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26 pages, 4071 KB  
Article
Hands-On Blockchain Teaching and Learning: Integrating IPFS and Oracles Through Open-Source Practical Use Cases
by Gabriel Fernández-Blanco, Pedro García-Cereijo, Tiago M. Fernández-Caramés and Paula Fraga-Lamas
Educ. Sci. 2025, 15(9), 1229; https://doi.org/10.3390/educsci15091229 - 16 Sep 2025
Viewed by 342
Abstract
The growing frequency of cybersecurity incidents, coupled with the increasing significance of blockchain technology in today’s digital landscape, highlights the urgent need for enriched, hands-on educational programs within Computer Science and Engineering curricula. While core blockchain curricula typically cover consensus protocols, smart contracts, [...] Read more.
The growing frequency of cybersecurity incidents, coupled with the increasing significance of blockchain technology in today’s digital landscape, highlights the urgent need for enriched, hands-on educational programs within Computer Science and Engineering curricula. While core blockchain curricula typically cover consensus protocols, smart contracts, and cryptographic foundations, more advanced topics like InterPlanetary File System (IPFS) and oracles pose teaching challenges due to their complexity and reliance on broader system knowledge. Despite this, their critical role in decentralized applications (dApps) justifies their inclusion at least through practical use cases. The integration of the IPFS protocol with Distributed Ledger Technologies (DLTs) can enable pure decentralized storage subsystems for dApps, avoiding single points of failure and ensuring data integrity and security. At the same time, as an external source of information, oracles are required to ensure data correctness while managing IPFS data. Despite the potential use of such components in real use cases, the current literature lacks detailed oracle implementations designed to interact with the IPFS protocol. To tackle such an issue, this article presents two open-source use cases that integrate smart contracts, an oracle and an IPFS-based storage subsystem that will allow future professors, students, researchers and developers to learn and experiment with advanced dApps and DLTs. Full article
(This article belongs to the Special Issue Perspectives on Computer Science Education)
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21 pages, 906 KB  
Article
Math Anxiety, Math Performance and Role of Field Experience in Preservice Teachers
by Bhesh Mainali and Danielle Spalding
Educ. Sci. 2025, 15(9), 1227; https://doi.org/10.3390/educsci15091227 - 16 Sep 2025
Viewed by 380
Abstract
This study investigates the causes of mathematics anxiety, its effect on mathematical performance, and the influence of field placement among preservice teachers enrolled in a field-based elementary mathematics methods course. To investigate this, the study utilized four tools: the Abbreviated Mathematics Anxiety Rating [...] Read more.
This study investigates the causes of mathematics anxiety, its effect on mathematical performance, and the influence of field placement among preservice teachers enrolled in a field-based elementary mathematics methods course. To investigate this, the study utilized four tools: the Abbreviated Mathematics Anxiety Rating Scale (A-MARS), concept maps, Praxis Core test score, and questionnaires. The quantitative analysis indicates a negative correlation between anxiety and math performance. The analysis of concept maps and questionnaires indicate that key contributors to math anxiety include pop-up quizzes, tests, exams, memorization of mathematical ideas, and past negative experiences with school mathematics. The qualitative data analysis revealed that reduced mathematics anxiety during field placements was primarily due to practical teaching experience, constructive feedback, positive student interactions, and opportunities for observation and reflection. Understanding the root causes of mathematics anxiety is essential for supporting preservice teachers and improving their teaching effectiveness. Additionally, field placements play a crucial role in reducing math anxiety by providing hands-on teaching experience and building confidence. It is important to alleviate math anxiety in preservice teachers to have a more positive impact on their future students. Full article
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18 pages, 778 KB  
Article
From Theoretical Navigation to Intelligent Prevention: Constructing a Full-Cycle AI Ethics Education System in Higher Education
by Xingjian Xu, Fanjun Meng and Yan Gou
Educ. Sci. 2025, 15(9), 1199; https://doi.org/10.3390/educsci15091199 - 11 Sep 2025
Viewed by 685
Abstract
The rapid integration of artificial intelligence (AI), particularly generative AI (Gen-AI), into higher education presents a critical challenge: preparing students for the complex ethical dilemmas inherent in AI-driven research and practice. Current AI ethics education, however, often remains fragmented, overly theoretical, and disconnected [...] Read more.
The rapid integration of artificial intelligence (AI), particularly generative AI (Gen-AI), into higher education presents a critical challenge: preparing students for the complex ethical dilemmas inherent in AI-driven research and practice. Current AI ethics education, however, often remains fragmented, overly theoretical, and disconnected from practical application, leaving a significant gap between knowing ethical principles and acting upon them. To address this pressing issue, this study proposes and validates a full-cycle AI ethics education system designed to bridge this gap. The system integrates three core components: (1) an updated four-dimensional ethics framework focused on Gen-AI challenges (research review, data privacy, algorithmic fairness, intellectual property); (2) a “cognition-behavior” dual-loop training mechanism that combines theoretical learning with hands-on, simulated practice; and (3) a full life-cycle education platform featuring tools like virtual laboratories to support experiential learning. A mixed-methods study with 360 students and 20 instructors demonstrated the system’s effectiveness, showing significant improvement in students’ ethical knowledge, a large effect size in enhancing ethical decision-making capabilities, and high user satisfaction. These findings validate a scalable model for AI ethics education that moves beyond passive instruction toward active, situated learning, offering a robust solution for higher education institutions to cultivate ethical responsibility in the age of Gen-AI. Full article
(This article belongs to the Topic AI Trends in Teacher and Student Training)
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60 pages, 923 KB  
Systematic Review
Virtual Reality in Higher Education: A Systematic Review Aligned with the Sustainable Development Goals
by David Llanos-Ruiz, Víctor Abella-García and Vanesa Ausín-Villaverde
Societies 2025, 15(9), 251; https://doi.org/10.3390/soc15090251 - 9 Sep 2025
Viewed by 1175
Abstract
Virtual Reality (VR) has emerged as a transformative tool in higher education, enabling immersive and interactive learning environments that support the assimilation of complex concepts, hands-on training, and innovative pedagogical practices. This systematic literature review analyzes studies published between 2020 and 2025 that [...] Read more.
Virtual Reality (VR) has emerged as a transformative tool in higher education, enabling immersive and interactive learning environments that support the assimilation of complex concepts, hands-on training, and innovative pedagogical practices. This systematic literature review analyzes studies published between 2020 and 2025 that examined the integration of VR in higher education and its connection with the United Nations Sustainable Development Goals (SDGs). Following the PRISMA guidelines, twelve studies were selected from the Web of Science and Scopus databases and assessed using predefined quality criteria. The findings highlight the predominance of mixed-methods approaches, with applications spanning diverse disciplines such as engineering, medical sciences, architecture, teacher training, and sustainability. The results emphasize VR’s potential to enhance student motivation, engagement, and digital competencies, while also contributing to Quality Education (SDG 4), along with other SDGs such as Good Health and Well-Being (SDG 3), Affordable and Clean Energy (SDG 7), Decent Work and Economic Growth (SDG 8), Reducing Inequalities (SDG 10), Sustainable Cities and Communities (SDG 11), and Climate Action (SDG 13). However, persistent challenges include high implementation costs, limited accessibility and teacher training, lack of standardization, and small short-term study designs. This review underscores the need for broader, longitudinal, and interdisciplinary research that integrates underrepresented SDGs and addresses inclusivity, equity, and long-term effectiveness, consolidating VR as a catalyst for innovation and sustainable development in higher education. Full article
(This article belongs to the Special Issue Neuroeducation and Emergent Technologies)
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6 pages, 1077 KB  
Proceeding Paper
Advancing Effective Climate Change Education by Using Remote Sensing Technologies: Leveraging the Research Infrastructure of the LAP/AUTh in Greece
by Konstantinos Michailidis, Katerina Garane, Chrysanthi Topaloglou and Dimitris Balis
Environ. Earth Sci. Proc. 2025, 35(1), 3; https://doi.org/10.3390/eesp2025035003 - 8 Sep 2025
Viewed by 3471
Abstract
Raising awareness and understanding of climate change among younger generations is crucial for building a sustainable future. The Laboratory of Atmospheric Physics (LAP) within the School of Physics of the Aristotle University of Thessaloniki (AUTh) supports this goal by developing innovative educational activities [...] Read more.
Raising awareness and understanding of climate change among younger generations is crucial for building a sustainable future. The Laboratory of Atmospheric Physics (LAP) within the School of Physics of the Aristotle University of Thessaloniki (AUTh) supports this goal by developing innovative educational activities centered on atmospheric processes and climate science. Drawing on its expertise in atmospheric monitoring and remote sensing, LAP makes complex scientific concepts accessible to school students through interactive workshops, hands-on experiments, and data-driven projects using real-time environmental measurements. By integrating research-grade tools and open-access satellite data from ESA, NASA, and EUMETSAT, LAP bridges academic research and public understanding. These activities foster critical thinking, environmental responsibility, and student engagement with real-world climate monitoring practices. Moreover, LAP contributes to the ACTRIS network, offering high-quality data and expertise at both national and European levels. Through these efforts, LAP serves as a hub for climate education, turning awareness into action and inspiring future climate-conscious citizens. Full article
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6 pages, 1456 KB  
Proceeding Paper
Action Research on Problem-Based Learning in Development of Engineering Curriculum in Design Departments: A Case Study of Mechanism Design
by Ting-Chien Lu
Eng. Proc. 2025, 103(1), 26; https://doi.org/10.3390/engproc2025103026 - 8 Sep 2025
Viewed by 2273
Abstract
Mechanism design, a fundamental course of the Department of Design, presents a significant challenge in learning product design. To address this issue, problem-based learning (PBL) was applied to teaching the “tree-climbing mechanism.” In the course, students designed and constructed a gear-based phone holder, [...] Read more.
Mechanism design, a fundamental course of the Department of Design, presents a significant challenge in learning product design. To address this issue, problem-based learning (PBL) was applied to teaching the “tree-climbing mechanism.” In the course, students designed and constructed a gear-based phone holder, assembled and evaluated a linkage mechanism using LEGO, designed and prototyped a tree-climbing mechanism through hands-on modeling, and tested the tree-climbing mechanism with handcrafted models or LEGO, incorporating batteries and motors. In each subtopic, students leveraged the department’s digital fabrication equipment and workshop to create functional prototypes. The instructor provided foundational knowledge of mechanisms to guide students to relevant resources and adapt the course content based on student reflections and assessments. Full article
(This article belongs to the Proceedings of The 8th Eurasian Conference on Educational Innovation 2025)
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26 pages, 1373 KB  
Article
Raising Climate Heroes: Ecological Game Camp—A Mixed-Methods Study on Experiential Climate Education in Children and Adults
by Canan Demir Yıldız
Sustainability 2025, 17(17), 8043; https://doi.org/10.3390/su17178043 - 6 Sep 2025
Viewed by 990
Abstract
This mixed-method study explores the impact of the Raising Climate Heroes: Ecological Game Camp on climate change knowledge, awareness, behavior, and emotional engagement among primary school students and adult participants. Designed with experiential and game-based learning approaches, the program aimed to enhance environmental [...] Read more.
This mixed-method study explores the impact of the Raising Climate Heroes: Ecological Game Camp on climate change knowledge, awareness, behavior, and emotional engagement among primary school students and adult participants. Designed with experiential and game-based learning approaches, the program aimed to enhance environmental literacy through interactive, nature-centered activities. The quantitative findings from pre- and post-tests revealed significant increases in climate-related knowledge, awareness, climate-friendly behavior, hope, and reductions in climate anxiety. All measurement tools demonstrated high internal consistency (α = 0.809–0.914), indicating strong reliability across both age groups. Qualitative data, analyzed using descriptive thematic analysis, showed high levels of participant satisfaction. The adults emphasized educational gains, professional relevance, and appreciation of academic facilitation. The children focused on enjoyment, outdoor experiences, and social interaction. Activities such as ecological experiments, composting, and collaborative cooking were most favored. The results suggest that combining cognitive and emotional elements through play and hands-on learning can effectively promote pro-environmental attitudes. This study contributes to the literature by demonstrating how climate education can be both engaging and transformative for diverse learner groups. Full article
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18 pages, 24627 KB  
Article
Enhancing Heritage Education Through ICT: Insights from the H2OMap Erasmus+ Project
by Delia Trifi, Pablo Altaba, Paloma Barreda-Juan, Guillem Monrós-Andreu, Laura Menéndez, Juan A. García-Esparza and Sergio Chiva
Educ. Sci. 2025, 15(9), 1164; https://doi.org/10.3390/educsci15091164 - 5 Sep 2025
Viewed by 565
Abstract
This study explored the Erasmus+ project ’H2OMap: Innovative Learning by Hydraulic Heritage Mapping’, integrating environmental awareness and cultural heritage into secondary education through interdisciplinary, ICT, and STEM-based approaches. Focused on water-related heritage in the Mediterranean, the study pursued three aims: integrate ICT-supported participatory [...] Read more.
This study explored the Erasmus+ project ’H2OMap: Innovative Learning by Hydraulic Heritage Mapping’, integrating environmental awareness and cultural heritage into secondary education through interdisciplinary, ICT, and STEM-based approaches. Focused on water-related heritage in the Mediterranean, the study pursued three aims: integrate ICT-supported participatory mapping bridging history/geography subjects with digital innovation; identify learning benefits and implementation conditions; and generate transferable outputs and datasets for classroom reuse. Intellectual outputs include a methodological guide, an e-learning course, and an educational multiplatform comprising a mobile mapping app for in situ geocataloguing, an online database, and a geoportal with interactive StoryMaps. Evidence came from classroom testing across age groups, teacher feedback from the e-learning course, student mobilities in Spain, Italy, and Portugal, and platform usage records. More than 390 students and teachers participated, documenting over 100 hydraulic heritage elements. Additionally, dissemination through nine multiplier events and conferences reached over 550 external attendees. Findings show increased student engagement and ICT/GIS skills, clearer cross-curricular integration, and a replicable open workflow supported by structured coordination that strengthens school–university partnerships. Learner experience emphasised hands-on, place-based exploration and collaborative documentation of water heritage. Recommendations include using open geospatial standards, providing teacher training, and maintaining geoportals for classroom reuse. Full article
(This article belongs to the Special Issue STEM Synergy: Advancing Integrated Approaches in Education)
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16 pages, 2770 KB  
Article
Comparing DNA Isolation and Preparation Protocols for Dried Blood Spots in the Context of Genomic Newborn Screening
by Annelotte J. Duintjer, Sandra Imholz, Ingrid Pico-Knijnenburg, Adinda Heuperman, Hennie Hodemaekers, Eva S. Deutekom, Els Voorhoeve, Martijn E. T. Dollé and Mirjam van der Burg
Int. J. Neonatal Screen. 2025, 11(3), 75; https://doi.org/10.3390/ijns11030075 - 3 Sep 2025
Viewed by 528
Abstract
Due to rapid technical advancements and increasing cost-effectiveness, the potential application of next-generation sequencing (NGS) in newborn screening (NBS) has raised great interest worldwide. Genomic NBS offers the possibility to improve current NBS programs when applied as follow-up tier, and, as first-tier, allows [...] Read more.
Due to rapid technical advancements and increasing cost-effectiveness, the potential application of next-generation sequencing (NGS) in newborn screening (NBS) has raised great interest worldwide. Genomic NBS offers the possibility to improve current NBS programs when applied as follow-up tier, and, as first-tier, allows for inclusion of conditions lacking a detectable biomarker for conventional NBS. Obtaining enough high-quality DNA from typically limited dried blood spot (DBS) material to meet NGS requirements can be challenging. Selecting a DNA isolation method for genomic NBS requires balancing technical performance and laboratory feasibility with optimal cost-effectiveness. Ten DNA isolation protocols, including two column-based, five lysis-based, and three semi-automated magnetic bead-based protocols, were evaluated on technical outcomes and performance in targeted amplicon sequencing. Additionally, estimated costs, hands-on time, turnaround time, scalability, and plastic footprint were assessed. Although technical outcomes, including yield, purity, and molecular weight, differed between methods, qualitative results in amplicon sequencing, as defined by read output, mapping, and coverage depth, were found sufficient and comparable for various protocols. In conclusion, both technical requirements and operational parameters are crucial when selecting a DNA isolation protocol and will depend on the NGS application as well as the NBS approach, as either first-tier or follow-up tier. Full article
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6 pages, 793 KB  
Proceeding Paper
Hands-On Training Framework for Prompt Injection Exploits in Large Language Models
by Sin-Wun Chen, Kuan-Lin Chen, Jung-Shian Li and I-Hsien Liu
Eng. Proc. 2025, 108(1), 25; https://doi.org/10.3390/engproc2025108025 - 3 Sep 2025
Viewed by 1419
Abstract
With the increasing deployment of large language models (LLMs) in diverse applications, security vulnerability attacks pose significant risks, such as prompt injection. Despite growing awareness, structured, hands-on educational platforms for systematically studying these threats are lacking. In this study, we present an interactive [...] Read more.
With the increasing deployment of large language models (LLMs) in diverse applications, security vulnerability attacks pose significant risks, such as prompt injection. Despite growing awareness, structured, hands-on educational platforms for systematically studying these threats are lacking. In this study, we present an interactive training framework designed to teach, assess, and mitigate prompt injection attacks through a structured, challenge-based approach. The platform provides progressively complex scenarios that allow users to exploit and analyze LLM vulnerabilities using both rule-based adversarial testing and Open Worldwide Application Security Project-inspired methodologies, specifically focusing on the LLM01:2025 prompt injection risk. By integrating attack simulations and guided defensive mechanisms, this platform equips security professionals, artificial intelligence researchers, and educators to understand, detect, and prevent adversarial prompt manipulations. The platform highlights the effectiveness of experiential learning in AI security, emphasizing the need for robust defenses against evolving LLM threats. Full article
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