Search Results (76,847)

The dextran sodium sulfate (DSS) colitis model is the most widely used experimental model of inflammatory bowel disease (IBD) due to its simplicity and versatility, with over 7000 PubMed entries in the last decade and an exponential rise in recent years. Since its initial description in 1985, DSS colitis has been extensively evaluated across species, most notably in mice and rats, and has yielded substantial insights into IBD pathogenesis. However, the model’s multifactorial nature poses a dual challenge: it offers an opportunity but complicates study design, interpretation, and translational relevance. This complexity is worsened by inconsistent reporting, which hampers reproducibility and comparability across studies. The broad use of the DSS-induced colitis model yields numerous insights about the model, which help better understand its complexity, characteristics and limitations. Although DSS colitis is induced locally, inflammation in the colon and gut barrier destruction may also affect other organs (such as the liver and brain) and their metabolism and molecular responses, which, in turn, may interfere with colitis-underlying mechanisms and drug response, and may influence the interpretation of results. These intrinsic (intra-experimental) characteristics of the DSS model are summarised in the paper (colitis, gut–brain axis, gut–liver axis). In addition, the DSS model is heavily influenced by numerous extrinsic (inter-experimental) factors (environmental, microbiological, genetic), which may further complicate the colitis model, the study outcomes, and data interpretation, and these are also discussed in the paper. As science advances and new data accumulate, understanding the intricate interplay among internal mechanisms, external factors, and technical variables becomes increasingly essential for the accurate interpretation of DSS outcomes. This review synthesises the complexity and interdependence of factors shaping the DSS model, emphasising the need for meticulous reporting and consideration of methodological nuances to enhance reproducibility, interpretation, and translational value in DSS colitis research. In addition, the review provides practical guidance through a “traps and tricks” subsection and checklist table designed to provide a framework and practical recommendations to better understand, apply, and interpret DSS model results in the context of broader systemic and methodological considerations. Full article

The onset of the COVID-19 pandemic prompted the rapid development and deployment of novel strategies and methodologies to manage the dissemination of microorganisms. Understanding the crucial role that contaminated surfaces play in the spread of viruses highlights the importance of having effective cleaning and disinfection protocols in place for inanimate objects. A variety of antimicrobial agents have shown strong effectiveness against the SARS-CoV-2 virus. Various factors can impact on the performance of these agents. As a result, technologies utilizing ozone’s microbicidal effects have been developed or improved for cleaning indoor areas, surfaces, and materials, despite ozone’s diverse uses being known for years. Ozone offers the advantage of adaptability for both gaseous and aqueous use, depending on the nature of the decontaminated surfaces. Moreover, ozone-infused water is ecologically benign, possesses microbial-fighting capabilities, and synergistically reinforces the biocidal action of other chemical disinfectants. This review aims to summarize the efforts dedicated to harnessing gaseous and aqueous ozone as a valuable means to eliminate the SARS-CoV-2 virus from environments, surfaces, clinical equipment, and office supplies. This review sourced evidence-based articles from electronic databases, including MEDLINE (via PubMed), EMBASE, the Cochrane Library (CENTRAL), and preprint repositories. The findings illustrated that ozone could serve as an additional tool for curbing the proliferation of COVID-19 and other viral infections. Additionally, we elucidated the operational attributes of ozone, the variables that influence its disinfection potency, and the mechanisms of its virucidal action. Notably, this review does not encompass the disinfection of the COVID-19 virus in wastewater. Full article

Lower-limb strength is a health and performance indicator in adolescents, although its assessment often fails to account for the influence of sex and biological development. This study aimed to analyze the associations between anthropometric parameters, maturational status, and physical activity levels with jumping performance in adolescents. A cross-sectional study was conducted with male and female adolescents (mean age: 13.60 ± 1.50 years). Anthropometric variables, maturational status, and physical activity levels were assessed. Performance was measured using the Countermovement Jump (CMJ) and Standing Broad Jump (SBJ). In males, jumping performance was significantly associated with height (p = 0.002), lower-limb length (p < 0.001), and muscle mass (p < 0.001). However, fat mass emerged as a substantial factor, exhibiting a large effect size on performance (p < 0.001). Maturational status in males showed significant differences, with late maturers performing lower than on-time and early maturers (p < 0.023). In females, structural anthropometry and maturation showed limited-to-no significant associations with performance, except for a negative association with fat mass (p < 0.035) and a positive association between muscle mass and CMJ (p < 0.020). Active adolescents of both sexes performed significantly better than inactive ones in both CMJ and SBJ (p < 0.011). In conclusion, jumping performance in adolescents is characterized by marked sexual dimorphism. In males, greater height, lower-limb length, lower fat mass, and early maturation are positively associated with superior performance. Conversely, in females, these factors exhibit limited influence on jump outcomes. Full article

Aging is a complex physiological process influenced by various factors, including individuals’ mental attitude. This interaction between biological vulnerability and psychological resources characterizes the entire life course; however, in older age, it becomes particularly salient due to the higher prevalence of multimorbidity, frailty, functional decline, and existential transitions (e.g., retirement, bereavement, loss of social roles), which intensify the impact of mental outlook on adaptation and quality of survival. Optimism has gained growing attention in clinical practice as a psychological asset associated with better health. This has also encouraged the incorporation of optimism-enhancing strategies into geriatric care. However, encouraging optimism in older patients, although well intentioned, can create ethical tensions in clinical communication, decision-making, and care planning. Sensitivity should be paid to aspects such as education, cultural background and religion within interactions with older adult patients. Uncritical promotion of optimism can undermine autonomy, foster unrealistic expectations, or place emotional burdens on patients who may already feel vulnerable. The appeal of optimism should therefore be balanced with careful ethical consideration. Full article

Pleural Mesothelioma (PM) is a rare, incurable malignancy of the pleura. Lung-sparing surgery, considered investigational, aims to prolong survival and improve quality of life (QOL). Beyond the standard quantitative measures used to determine successful surgical outcomes, an understanding of an individual’s perception of the impact surgery has had on their symptom burden and QOL has not been reported in the literature. The primary aim of this study was to explore the lived experience of dyspnea and QOL before and after lung-sparing surgery. The philosophical approach to this study was grounded in hermeneutical phenomenology. Participants underwent in-depth semi-structured interviews before and 3–4 months post-surgery, analyzed through Interpretive Phenomenological analysis. The analysis identified Group Experiential Themes (GETs) before and after surgery: Psychological (mind supports body), Physiological (body fighting, enduring, and adapting), Social (others sharing and supporting), and Existential (facing an uncertain future). The emotional impact of PM is multidimensional, involving time, internal psychological struggles, and coping with the diagnosis. The physical impact disrupts normal routines and interactions, while social interactions influence the perception of the illness experience. Facing PM disrupts normal bodily routines and interactions with the world. This study provides qualitative evidence that perceptions of dyspnea and QOL significantly impact the patient experience before and after surgery. The enriched understanding of living with mesothelioma and enduring lung-sparing surgery comes from the patients’ voices, highlighting the continuum of dyspnea and QOL influenced by various factors. Healthcare teams must consider patients’ physical, emotional, social, and existential experiences beyond measurable outcomes. Full article

Objective: Influenza and pertussis vaccines are recommended during pregnancy; however, uptake remains insufficient in many European countries, increasing the risk of preventable infections. Recent recommendations for maternal respiratory syncytial virus vaccination have been endorsed by scientific societies. This study evaluated maternal vaccination coverage, knowledge, attitudes, and factors influencing vaccine uptake among Lithuanian women. Methods: A retrospective cross-sectional online survey was conducted between 4 and 14 November 2025 in Lithuania among women aged 18–55 years with at least one previous pregnancy. The questionnaire contained 29 questions on sociodemographic characteristics, obstetric history, vaccination history, attitudes, and informational sources influencing decisions. Internal reliability was confirmed (Cronbach’s α = 0.83). Descriptive statistics were used to summarize the data. Associations between categorical variables were assessed using the Chi-square test or exact tests (Fisher’s exact or Fisher–Freeman–Halton). Binary and multivariable logistic regression analyses were performed to evaluate factors associated with self-reported vaccination uptake and the relationship between influenza and pertussis vaccination. Odds ratios with 95% confidence intervals were calculated. Statistical significance was set at p < 0.05. Results: A total of 241 women participated. Self-reported vaccination coverage during pregnancy was 28.7% for influenza, 43.8% for tetanus–diphtheria–pertussis, and 4.2% for respiratory syncytial virus. Physician’s recommendation was the strongest predictor: women advised to vaccinate were 17.0 times more likely to receive influenza, 16.5 times more likely to receive pertussis, while RSV vaccination occurred almost exclusively among women who reported receiving a physician’s recommendation. Higher uptake was associated with younger maternal age and university education. Reasons for declining vaccination were avoidance of medical interventions and concerns about safety or side effects. Conclusions: Maternal vaccination coverage in Lithuania remains low despite public funding and national recommendations. Strengthening provider communication, improving information strategies, and integrating vaccination counseling into routine antenatal care may increase uptake and enhance maternal and neonatal protection. Full article

The spatiotemporal evolution of ecosystem services and the elucidation of their driving mechanisms constitute a central scientific issue in territorial spatial optimization and regional sustainable development. Taking Gansu Province, a core area of the ecological security barrier in northwestern China, as the study area, this study integrates land-use, natural geographic, and socioeconomic data from 2000 to 2020. Using a land-use transfer matrix, the InVEST model, the Geographical Detector, and the PLUS model, we constructed a comprehensive analytical framework that combines historical evolution analysis, spatial differentiation identification, and multi-scenario simulation and prediction. The framework was used to systematically reveal the spatiotemporal dynamics of four core ecosystem services, namely carbon storage (CS), water yield (WY), habitat quality (HQ), and soil retention service (SDR), and to analyze their natural and socioeconomic driving mechanisms, while also simulating land-use change and ecosystem-service responses under the natural development, ecological protection, and urban expansion scenarios in 2030. The results show that, from 2000 to 2020, land use in Gansu Province was dominated by grassland (average proportion: 33.34%) and unused land (average proportion: 41.35%). Urban land expanded from 660.52 km² to 2227.36 km², with its share increasing from 0.15% to 0.50%, mainly through the conversion of cropland and grassland. Ecosystem services exhibited marked spatial differentiation: CS increased from east to west; WY showed an increasing pattern from northwest to southeast; HQ was lower in the central and southeastern regions and higher in the western and southern regions; and SDR was dominated by low-value areas in the northwest (average proportion: 84.81%). Driving-mechanism analysis indicated that slope was the core natural factor affecting CS, HQ, and SDR (q = 0.18–0.45), while mean annual precipitation dominated the variation in WY (q = 0.31–0.35). The influence of socioeconomic factors such as GDP increased gradually over time, showing an evolutionary trend from natural dominance to coordinated natural–socioeconomic regulation. Multi-scenario simulation further showed that, under the ecological protection scenario, grassland area increased significantly (+0.60%), the proportions of medium-value CS zones and high-value WY zones increased, and ecosystem services were optimized overall; under the urban expansion scenario, cropland and urban land expanded (+0.87% and +0.23%, respectively), imposing potential pressure on part of the ecosystem-service functions. These findings provide a scientific basis for optimizing territorial spatial planning, strengthening the ecological security barrier, and promoting regional sustainable development in Gansu Province. The methodological framework also offers a broadly applicable reference for ecologically sensitive arid and semi-arid regions in northwestern China. Full article

Background: Parental practices that support autonomy, provide structure, and foster warm relationships are associated with greater satisfaction of students’ basic psychological needs for autonomy, competence, and relatedness. In minority educational contexts, however, students’ psychological need satisfaction is also shaped by broader sociocultural conditions that may create additional pressures and sources of chronic stress. Within such environments, parental support may function as a protective factor that helps students cope with educational and cultural demands. Objective: The aim of this study was to explore how parental support contributes to the satisfaction of students’ basic psychological needs within a minority educational context where students from the Greek minority attend a bilingual school operating within a Turkish educational framework. Methods: A qualitative design was employed using three focus groups conducted in a minority school located in Gökçeada, Türkiye: one with parents (N = 5), one with lower secondary school students (N = 6), and one with upper secondary school students (N = 6). Interview questions were developed on the basis of Basic Psychological Needs Theory. Data were analyzed thematically by five members of the research team. Results: Findings indicated that parental support influenced students’ need satisfaction through practices related to autonomy (e.g., trust, space for mistakes), competence (e.g., encouragement, comparison), and relatedness (e.g., emotional presence, empathy). However, these practices were not experienced in a uniform way. Rather, their meaning and impact were shaped by contextual conditions associated with minority status, including bilingual educational demands, limited resources, and close-knit community dynamics. Conclusions: The study suggests that in minority school settings, parental support operates not simply as a general interpersonal resource but as a contextually mediated protective process. By showing how sociocultural and institutional conditions shape the enactment and experience of autonomy, competence, and relatedness, the findings extend existing BPNT research beyond majority settings and offer a more context-sensitive understanding of students’ psychological need satisfaction. Full article

Despite the growing adoption of Ti6Al4V-ELI made by Laser Powder Bed Fusion (LPBF) in tribologically demanding applications, the influence of hybrid nanoparticle additives on its lubrication behavior under starved contact conditions remains insufficiently explored. The tribological performance of Ti6Al4V was investigated under starved boundary-to-mixed lubrication conditions using engine oil modified with Ag-doped TiO₂ nanoparticles. Double-scan LPBF-fabricated discs were tested in a ball-on-disc configuration against AISI 52100 bearing steel using a TRB³ tribometer. Nanolubricants were prepared by dispersing TiO₂ and Ag–TiO₂ nanopowders with different Ag⁺/Ti⁴⁺ ratios (0.5%, 1.5%, and 2.5%) in SAE 10W-40 engine oil at a constant nanoparticle concentration of 0.05 wt%. Comprehensive physicochemical characterization of the nanopowders and nanolubricants was performed through structural, chemical, optical, morphological, rheological, and stability analyses. Tribological experiments were conducted following a full-factorial design combining three normal loads (5–15 N), three sliding speeds (0.10–0.20 m·s⁻¹), and four lubricant formulations. The steady-state coefficient of friction ranged between 0.281 and 0.359, while the specific wear rate varied from 2.81 × 10⁻⁴ to 4.83 × 10⁻⁴ mm³·N⁻¹·m⁻¹. The contact temperature rise remained relatively moderate, within the interval of 1.9–9.4 °C. Among the investigated formulations, the lubricant containing 1.5% Ag–TiO₂ exhibited the lowest friction coefficient, whereas the formulation with the highest Ag content showed improved stability of tribological performance across the investigated operating domain. These results indicate that Ag-modified TiO₂ nanoparticles are consistent with the formation of protective tribofilms and contribute to the stabilization of friction, wear, and thermal behavior under starved lubrication conditions. ANOVA confirmed that sliding speed and the load–lubricant interaction are the dominant factors governing friction and wear, while normal load controls the thermal response. These findings support the use of Ag–TiO₂ nanolubricants as a viable strategy for stabilizing interfacial behavior in LPBF-fabricated titanium components operating under starved lubrication conditions. Full article

Indonesia has identified clean hydrogen as one of the strategic initiatives for its energy transition, recognizing its potential as an energy carrier that can support the achievement of net zero emissions. To deepen the understanding of this emerging technology, this study assesses the readiness of green hydrogen development in Indonesia through a multi-stakeholder perspective combined with a technology readiness evaluation and insights from global developments. Based on stakeholder interviews across government, industry, academia, and energy institutions, this analysis identifies key enabling conditions and barriers for hydrogen deployment in the Indonesian context. This analysis indicates that the readiness level of green hydrogen technology in Indonesia has reached approximately technology readiness level (TRL) 5–TRL 6, suggesting that most initiatives remain at the pilot and demonstration stages. In addition, seven key factors influencing green hydrogen adoption were identified: infrastructure and technology, policy and regulation, finance, application sectors, public acceptance, standardization, and private sector participation. These results provide policy-relevant insights for accelerating hydrogen development and highlight priority areas for advancing Indonesia’s transition toward a low-carbon energy system. Full article

Background/Objectives: Stress experienced by families of individuals with illnesses or disabilities is shaped through the interaction of multiple complex influencing factors. This study aimed to elucidate the factors influencing stress in families of young/adult children with illnesses or disabilities, using Family System Unit Stress Theory (FSUST) as the guiding theoretical framework. Methods: Semi-structured interviews were conducted with 10 families of young/adult children with illnesses or disabilities. Data were analyzed using qualitative content analysis following the approach of Graneheim and Lundman. In line with FSUST, the identified influencing factors were categorized into negative factors (risk/causal/promoting) and positive factors (preventive/inhibitory/suppressive). Results: A total of six categories and 18 subcategories were extracted for the risk/causal/promoting factors, including “accumulation of unshared burdens within the family leading to role overload” and “concerns about the future of the young/adult child.” For the preventive/inhibitory/suppressive factors, five categories and 13 subcategories were extracted, including “receiving a diagnosis of the young/adult child’s illness or disability” and “family maintaining a positive attitude.” Conclusions: Family stress in families of young/adult children with illnesses or disabilities varies through the interaction of multilayered influencing factors, including persistent emotions carried over from the past, difficulties faced in the present, and anticipatory concerns regarding the future. Therefore, nursing practice requires a life course-oriented understanding of family stress and an integrated approach that concurrently reduces risk/causal/promoting factors while enhancing preventive/inhibitory/suppressive factors. Full article

Polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene (B[a]P), are major risk factors for lung cancer and other diseases, acting through the aryl hydrocarbon receptor (AHR). Alveolar macrophages (AMs) help regulate the lung microenvironment by responding to inhaled toxicants and resident microbiota. Although small extracellular vesicles (sEVs, aka exosomes) released by AMs mediate intercellular communication and immune responses, the influence of lung microbiota on sEV biogenesis and the mechanisms underlying sEV dysregulation during PAH exposure remain unknown. Here, we investigated the interplay between AMs, B[a]P, and lung microbiota, focusing on sEV-associated miRNAs (exo-miRNAs). Murine AMs (MH-S) were exposed to varying B[a]P concentrations in the presence or absence of murine lung microbiota with or without an AHR antagonist. sEVs from each condition were characterized and profiled for miRNA. Distinct miRNA signatures emerged: high-dose B[a]P enriched miRNAs linked to cancer progression, whereas lung microbiota alone or with low-dose B[a]P induced tumor-suppressor miRNAs that limit proliferation and metastasis and promote apoptosis, an effect enhanced by AHR antagonism. Lung microbiota appeared to counteract high-dose B[a]P by modulating tumor-suppressive exo-miRNAs. This study demonstrates that lung microbiota-induced exo-miRNAs critically shape AM-derived sEV-miRNA signaling during PAH exposure. The identified exosomal miRNAs could serve as important exposure biomarkers and therapeutic targets for mitigating B[a]P-induced toxicity and cancer development. Full article

As a crucial national ecological barrier, China’s Southern Collective Forest Region (SCFR) plays an essential role in maintaining regional ecological security and promoting sustainable development. Understanding the mechanisms driving the evolution of its ecosystem service value (ESV) is of great significance. Based on county-level data from 2000 to 2023, this study integrated the equivalent factor method, spatial autocorrelation analysis, the XGBoost-SHAP model, geographically and temporally weighted regression (GTWR), and partial least squares structural equation modeling (PLS-SEM) to examine the spatio-temporal evolution patterns and driving mechanisms of ESV in the SCFR. The results showed that ESV in the SCFR exhibited an overall downward trend, with a cumulative loss of 1973.77 × 10⁸ CNY. This was primarily due to marked reductions in hydrological and climate regulation services. The spatial distribution of ESV exhibited a significant heterogeneity—higher in the southwestern and southeastern mountainous regions, and lower in the northern plains and coastal zones, with the center of gravity shifting first to the northeast and then to the southwest. Local spatial autocorrelation revealed relatively stable “High–High” and “Low–Low” clustering characteristics, where high-value clusters were consistently distributed in core forest zones, while low-value clusters overlapped highly with urban agglomerations. Socio-economic factors exerted a significantly stronger influence on ESV than natural factors. Population density (POP), land use intensity (LUI), and gross domestic product (GDP) were identified as the dominant drivers, exhibiting distinct non-linear threshold effects and significant spatio-temporal heterogeneity. PLS-SEM analysis further quantified LUI as the dominant direct inhibitory pathway on ESV, highlighting urbanization’s indirect negative effect mediated through intensified LUI. Meanwhile, terrain effects were confirmed to positively influence ESV indirectly by constraining LUI and modulating local climate. The analytical framework of “threshold identification–spatio-temporal heterogeneity–causal pathway analysis” proposed in this study elucidated the complex driving mechanisms of ESV evolution, providing valuable guidance for ecological restoration evaluation and differentiated environmental governance. Full article

Aim: The aim of this study was to explore the distribution and driving factors influencing the disparity of health professionals (HPs) at the Centers for Disease Control and Prevention (CDC) in China and to provide a reference for regional health planning and rational allocation of public health resources. Methods: The Gini coefficient was used to measure the equity of HP distribution at CDC sites at the provincial level during 2012–2023 in China. Moran’s I was used to analyze the spatial agglomeration effect, and the geographic detector model was used to explore the factors driving the allocation of HPs at CDC sites in different provinces. Results: The number of HPs at the CDC showed an increasing trend from 2012 to 2023 in China. The average Gini coefficients at the population and geographical areas were 0.16 and 0.58, respectively. The global Moran’s I statistic indicated a notable decline in spatial clustering for the population dimension, decreasing from 0.503 to 0.238; in contrast, spatial clustering for the geographical dimension remained relatively stable, ranging between 0.13 and 0.16. The local Moran’s I statistic revealed that provinces such as Qinghai in the western China consistently exhibited a “low–low” spatial clustering pattern. Six factors were found to explain the variability in the CDC HP distribution based on the 2020 data. In the context of factor interactions, the synergistic effects between education level and health expenditure share (q = 0.9781), and between population aging and per capita GDP (q = 0.9699), substantially exceed the explanatory power attributable to any single factor alone. Conclusions: A significant regional disparity was observed in the distribution of HPs among 31 provinces, with the distribution based on service area being less equitable than that based on population. The shortage of healthcare professionals in the western region is characterized by notably inadequate geographical distribution. Future policy initiatives should prioritize targeted spatial interventions and integrated, multi-factor collaborative strategies. Full article

Natural killer (NK) cells are effector cells of the innate immune system. The cytokine microenvironment influences NK cell function. Dysregulation of NK cell cytotoxicity can manifest in reproductive disorders and is also observed in tumor-transformed tissues. The search for immunotherapies capable of regulating NK cell activity is therefore relevant. This study aimed to evaluate the effect of the TGFβ signaling pathway inhibitor and the cyclin-dependent kinase (CDK) 7/12/13 inhibitor on the transcriptional profile of NK-92 cell line. In the study, the cytokines TGFβ1, IL-12, IL-15, IL-18, and TNFα, and the TGFβ receptor type 1 (TGFβR1) inhibitor LY3200882 and the CDK7/12/13 inhibitor THZ1 were used. The cells were cultured sequentially in the presence of inhibitors and cytokines, followed by assessment of the gene expression of NCR2, NCR3, AHR, NCAM1, B3GAT1, EOMES, GATA3, KLRC1, KLRC2, CCL5, IL10 and TBX21. We observed direct effects of the inhibitors on NK cells. LY3200882 increased the expression of KLRC1 and B3GAT1, and reduced NCAM1. THZ1 increased the expression of KLRC1, KLRC2, AHR and EOMES, while it reduced IL-10 and NCR2. IL-12, IL-15, IL-18, and TNFα modified the gene expression of some phenotypic and cytotoxic receptors and transcription factors. TGFβ1 increased the expression of KLRC1, NCAM1, and B3GAT1. Blocking TGFβ-dependent signaling with LY3200882 abolished TGFβ1 effects. We assessed CD56 presence on NK-92 cell membrane and found its increase in the presence of LY3200882. After LY3200882 treatment, in the presence of TGFβ1 and choriocarcinoma cell line JEG-3, the expression of CD56 receptor on NK cell membrane decreased. Pretreating NK cells with THZ1 decreased the expression of NCAM1, B3GAT1, and EOMES in the presence of TGFβ1. Thus, LY3200882 partially neutralized TGFβ1 effects on the expression of NK cell receptor genes. THZ1 followed by TGFβ1 treatment promoted NK cell transcriptional profile characteristic for CD56dim NK cells. Both LY3200882 and THZ1 affected the NK cell transcription even without cytokine treatment. The independent effects of synthetic inhibitors on NK cells, as well as their influence in the presence of tumor cells, should be considered. Full article