Search Results (93)

This article investigates the role of space in the fiction of Jeffrey Eugenides, focusing on The Virgin Suicides (1993) and Middlesex (2002) through the lens of spatial theory. Drawing on key thinkers such as Henri Lefebvre, Michel Foucault, Edward Soja, Yi-Fu Tuan, and Doreen Massey, the study explores how Eugenides constructs spatial environments that not only frame but actively shape the identities, desires, and traumas of his characters. In The Virgin Suicides, suburban domestic spaces are shown to function as heterotopias—sites of surveillance, repression, and mythologized femininity—while Middlesex engages with transnational and urban spaces to narrate diasporic and intersex identity as dynamic, embodied, and liminal. The analysis reveals that Eugenides uses space as both a narrative device and a thematic concern to interrogate gender, memory, and power. Rather than passive backdrops, the novelistic spaces become charged arenas of conflict and transformation, reflecting and resisting dominant socio-cultural discourses. This study argues that space in Eugenides’ fiction operates as a critical register for understanding the politics of belonging and the production of subjectivity. By situating Eugenides within the broader field of literary spatiality, this article contributes to contemporary debates in literary geography, gender studies, and American fiction. Full article

Gynandromorphism of mayfly has unique biological and scientific value. However, most cases of previous studies on this phenomenon were from Europe and America, with only two cases reported from China. In this study, we examined the mayfly collections in our laboratory and obtained 21 intersex specimens belonging to three families (Baetidae, Leptophlebiidae, and Siphlonuridae) and seven species (Baetis rutilocylindratus, three unnamed Baetis species, Neoleptophlebia sp., Choroterpes facialis, and Siphlonurus lacustris), which greatly enrich the diversity of mayfly gynanders of China. Biogeographically, this phenomenon appears cosmopolitan. Regarding the potential causes of mayfly gynandromorphism, we hypothesize that inorganic factors may play a significant role. Full article

Gender-independent, intersex, non-binary, and transgender (GIaNT) youth have puberty health and sex education needs that are different from cisgender youth. Unfortunately, this population has historically been excluded from curricula pertaining to these issues in schools throughout the U.S. and Canada. While sex education programs are limited for all youth, and navigating puberty can range from exciting to scary for all youth, GIaNT youth are particularly vulnerable to facing negative outcomes by being omitted from such curricula. Because they are not able to access adequate information in schools and in their family/friend networks, a majority of GIaNT youth turn to the internet to seek out resources for education on puberty health and sex education. To this end, our team is in the process of creating an online educational resource (OER) that is age-appropriate and affirming for GIaNT youth to be provided with resources that are personalized to users’ unique needs. This literature review discusses content suggestions for inclusion of comprehensive puberty health and sex education that caters to GIaNT youth and their unique needs. Full article

Certain bodily functions are generally associated with distinct sexes and genders. When it comes to intersex individuals, the gendering of certain bodily functions as male or female plays a key role in determining their sexed corporeality and self-determination of gender. The article elucidates how the protagonist Ash in Just Ash (2021) by Sol Santana is oppressed under notions of menstrunormativity due to his menstruating body. It explains how the gendering of a bodily function like menstruation as feminine plays a significant role in the medical interventions on intersex individuals and how the same norms affect intersex individuals’ own perceptions of themselves. Ash, who identifies himself as a boy, is bullied by his peers at school, and his parents impose the female sex and feminine gender identity on him because of his menstruating corporeality, all leading to Ash’s own negative perception of his body. The article also highlights the importance of degendering menstruation to include sex- and gender-diverse individuals. Full article

The literature details the healthcare needs of migrant people living with chronic illnesses and the consequent economic, social, and healthcare needs of their caregivers. Similarly, some studies have underscored the social and healthcare needs of 2SLGBTQIA+ (two-spirit, lesbian, gay, bisexual, transgender, queer, and intersex individuals, including diverse sexual and gender identities under the “+” symbol) adults living with chronic illnesses and their caregivers. This narrative review presents the context of migrant 2SLGBTQIA+ unpaid caregivers and how their intersecting identities influence their caregiving roles for family members with chronic illnesses. In this article, caregivers are defined as family members or chosen families who provide unpaid support that may last for three months or longer for people living with chronic illnesses. Most studies and policies overlook 2SLGBTQIA+ migrants who are also unpaid caregivers of individuals living with chronic illnesses, leaving them unsupported through discrimination at the intersection of racism, homophobia, transphobia, ageism, and ableism, forcing them to remain vulnerable to increased emotional and physical strain. There is a presence of pervasive systemic barriers, including a lack of training and education among social and healthcare providers, about the needs of migrant 2SLGBTQIA+ unpaid caregivers. Additional challenges stem from inadequate policies and insufficient targeted resources, particularly for caregivers from marginalized racial and ethnic backgrounds. The findings of this study highlight the necessity for a call to action to address these gaps and improve support systems for these highly marginalized communities. Full article

Research with population groups who have undergone circumstances of vulnerability demands a careful ethical approach to ensure that the study is conducted with the utmost respect and sensitivity, addressing potential challenges not only in regards to safeguarding participants’ rights but also in preserving the well-being of researchers. This paper is based on a qualitative research project. Employing snowball sampling techniques, I contacted 21 participants by e-mail, comprising intersex individuals who had engaged in research, as well as researchers and ethics experts with experience in qualitative intersex-related studies. I conducted semi-structured interviews to gather insights from their respective experiences. The research article focuses on a set of ethical principles that could be valuable in the development of intersex-related studies. While certain principles are directed towards participants and others towards researchers, it is important to note that these categories are not strictly delineated. As the findings suggest, these categories are closely interrelated. Both the literature review and fieldwork underscore the significance of adopting various research methods, such as community-based and trauma-informed approaches, to collaborate in enhancing knowledge production for intersex-related studies. Full article

Congenital developmental abnormalities in piglets, such as intersex and aproctia, adversely affect survival rates, growth performance, and genetic breeding efficiency in pig populations. To elucidate their genetic basis, we performed a genome-wide association study (GWAS) on 1030 Large White pigs. We combined 50 K SNP chip data with SWIM-based genotype imputation to enhance the resolution of genetic variation detection, followed by MLM analysis. Our results identified 53 significant SNPs, with 52 associated with intersex and 1 with aproctia. Key candidate genes included MAD1L1, ID4, EFNA5, and PPP1R16B for intersex and ARNT2 for aproctia. Functional enrichment analysis highlighted pathways related to gonadal development (e.g., progesterone-mediated oocyte maturation) and embryonic morphogenesis. Collectively, the identification of these SNPs and candidate genes advances our understanding of the genetic architecture of intersex and aproctia in piglets. These findings provide actionable insights for optimizing genetic breeding strategies and improving health management in Large White pig production, with potential implications for reducing economic losses caused by congenital disorders. Full article

DNA methylation is a form of epigenetic regulation that plays an important role in regulating gene expression of organisms. However, the DNA methylation pattern of intersex crabs has not yet been clarified. In order to reveal the DNA methylation in intersex Eriocheir sinensis, this study investigated the genome-wide DNA methylation profiles of female, male, and intersex individuals. The similar results across samples showed that the levels of cytosine methylation in the CG context were significantly higher than that in the CHG and CHH contexts. The methylation levels in the promoter region were higher than those in other functional element regions. We screened 149 differentially methylated genes (DMGs) in the promoter region between female and intersex crabs and 110 DMGs between male and intersex crabs. Three core gene networks were found in a comparison group of female and intersex crabs that involved heat shock proteins, ribosomes, and metabolism pathways; two core gene networks were found in the comparison group of male and intersex crabs that involved ribosomes and metabolism pathways. The six confirmed genes of Hsc70, Hsp90, Rpl18, Acsl1, Yip2, and Rpl7 had lower methylation levels in the promoter region of intersex crabs than that of female and male crabs. However, six genes showed higher expression in intersex crabs than in female and male crabs. Our results reveal that DNA methylation is involved in the formation and maintenance of life activities of intersex crabs through the regulation of gene expression, enriching the DNA methylation library of the whole genome of E. sinensis and providing new insights for a better understanding of the epigenetic regulation of the formation of intersex E. sinensis. Full article

Sexual and gender minority (SGM) communities experience an increased risk for sexual victimization. Because these rates are reported in aggregate, little is known about the unique experiences of sexual violence among individuals with differences of sex development/intersex variations (DSD/I). The purpose of this study is to describe the experiences of sexual victimization in adolescents and young adults (AYAs) with DSD/I as part of a larger qualitative study exploring experiences of minority stress, stigma, and resilience. Participants were recruited through multidisciplinary care clinics and DSD/I support groups in the United States and the Netherlands. Data on sexual experiences and sexual health were analyzed thematically, with a specific focus on reports of sexual victimization. Of the 20 participants, 25% reported experiences of sexual violence. Experiences were reported across gender identities and DSD/I diagnoses. Participants described experiences as (a) resulting from DSD/I, (b) exacerbated by DSD/I, or (c) healthcare practices experienced as sexual violence. Medicalization, early loss of (bodily) autonomy, and cisgender heteronormative reproductive societal norms were reported as possible factors associated with sexual victimization experiences. The sexual victimization of individuals with DSD/I appears prevalent and associated with specific risk factors and, therefore, deserves clinical and scientific attention. Full article

The pursuit of sustainable protein is underway. This debate is often framed as a choice between two competing agrifood futures: the “no cow” and “clean cow” perspectives. The former comes from alternative protein advocates, while the latter aims to support practices, discourses, and livelihoods associated with regenerative ranching. The findings presented reveal greater nuance than what this simplistic dichotomy suggests. This paper utilizes data collected from fifty-eight individuals in California and Colorado (USA). Participants in the sample were identified by their attendance at various events focused on sustainability in protein production and includes a subsample of regenerative farmers who self-identified as persons of color, disabled or differently abled, and/or part of the LGBTQIA+ (Lesbian, Gay, Bisexual, Transgender, Queer/Questioning, Intersex, Asexual) community. The sample features a range of viewpoints associated with regenerative livestock and non-livestock protein production. The data support arguments aligned with “clean cow” framings, as determined by the anticipated scope of sustainable protein transformations. However, the paper cautions against solely relying on this frame without further interrogating its contours. It particularly notes that the values of specific “clean cow” actors and networks mirror key aspects of “no cow” perspectives. These similarities are especially evident among upstream actors like investors, corporate interests, and government sponsors. For these individuals and networks, the “no” versus “clean” distinction—despite suggesting radically different agrifood futures—overshadows underlying shared concerns that align with core elements of the status quo. A case is also made for greater reflexivity and, thus, inclusivity as we think about who is included in these debates, as the data tell us that this shapes how we frame what is at stake. Full article

A 2-year-old Large White research gilt was presented to the Comparative Theriogenology service at WSU for infertility. She was produced from the transfer of genetically modified embryos obtained via in vitro fertilization (IVF) that had been treated with CRISPR-Cas9 reagents to mutate the NANOS2 gene. Since birth, the gilt showed a so-called “skyhook” vulva and abnormal estrous cycles. She was artificially inseminated multiple times, starting at 5 months of age, with semen from a proven fertile boar, but never conceived. On transabdominal ultrasonography of the reproductive tract, a unilateral ovotestis was suspected. An exploratory laparotomy was performed and confirmed the presence of ovarian and testicular tissue on her right gonad. The diagnosis was confirmed by histology following unilateral gonadectomy. The cytogenic evaluation revealed the gilt to be XX 38, SRY-negative. The gilt showed estrus signs 4 months after surgery, and artificial insemination was performed, which resulted in pregnancy. She delivered six piglets. Necropsy was performed following humane euthanasia. Several abnormalities of the reproductive tract were discovered, including a unilateral mucometra due to a complete septum, preventing communication between the right uterine horn and the body of the uterus. This case is novel because it demonstrates that surgical removal is an effective treatment for fertility in SRY-negative XX DSD gilts with partial masculinization, posing the presence of a normal ovary and a developed oviduct and uterine horn. Full article

Walleye pollock (Gadus chalcogrammus) is a commercially important species widely distributed in cold-water regions. We have been culturing this species artificially since 2015. The average embryo diameter was 1.43 ± 0.056 mm, and hatching occurred at 5 °C approximately 339 h post-fertilization. Gonadal development became visibly apparent at a body size of 10–15 cm after ~180 days, initially distinguishing ovaries with the development of germ cells, whereas testes were observable after further maturation. We discovered two intersexes from F1 and F2 generations, and one abnormal mature individual from F2. Morphologically, intersex walleye pollock exhibited distinct characteristics of ovary and testes, with male gonads visibly connected to the end of ovaries. In intersex walleye pollock aged 3–6 years, the gonads developed normally, and oocyte resorption was restricted to the area near the connections between testicular and ovarian tissues, with numerous atretic oocytes observed in the resorption zone. Primordial germ cells were found together in individuals who had not undergone spawning, indicating an abnormal maturation pattern. Although no significant differences in the gonadosomatic index were observed between intersex and normal individuals, further research on intersexuality is necessary to understand the reproductive development of this species and the health of offspring spawned by intersex individuals, which are rare. Full article

Intersexuality is a reproductive phenomenon that occurs in some gonochoric species and refers to the simultaneous presence of both male and female gametes within the same individual. Although this phenomenon has been reported in various invertebrate species, many aspects remain poorly understood, especially in marine mussels. However, it has been suggested that the prevalence of parasites within populations could induce the occurrence of intersex animals. We studied intersexuality in the marine mollusk Perumytilus purpuratus, a key species of the rocky intertidal zone on the southeastern coast of the Pacific Ocean. A total of 6472 mussels from eight locations in northern and southern Chile were analyzed. We estimated the size of the specimens, the sex ratio of the population, and the prevalence of parasites. Additionally, we examined the germ cells of intersex mussels. The results showed that the male-to-female sex ratio (1:1) was maintained in the populations. Intersex mussels were found in six of the eight locations, representing 0.19% of the mussels analyzed. However, no parasites were found in the intersex animals. Additionally, the abundance of intersex individuals was not correlated with parasitism levels in the population. In intersex mussels, the gonadal tissue was compartmentalized, with male and female germ cells remaining separate. It is concluded that intersexuality in Perumytilus purpuratus is a low-frequency reproductive phenomenon, likely resulting from an alteration in the sex determination mechanism. Intersexuality offers a valuable opportunity to explore the biological aspects of sex determination in mussels. Therefore, further research in this area should be pursued. Full article

Dioecious plants have different needs for natural resources due to sex differences, which can lead to skewed sex ratios. Clonal growth facilitates and optimizes resources of clonal plants. So, dioecious plants show inter-sex differences in clonality. However, it is unclear how the clonality varies between female and male plants when they exhibit skewed sex ratios along an altitudinal gradient. Here, we investigated the sex ratio and clonality of Hippophae tibetana populations growing at three altitudes in the alpine meadow. We founded that (i) H. tibetana growing at different altitudes mainly consisted of II and III age classes, with a significantly male-biased sex ratio observed at a low altitude, a female-biased ratio at a middle altitude, and no significant sex-bias in the population at a high altitude. (ii) The population distribution was generally random at both low and high altitudes, while clustered at middle altitudes with an increasing scale. Meanwhile, the male and female populations at a low altitude showed a negative correlation, while the male and female at a middle altitude only showed a negative correlation at a 0–0.5 m scale, and spatial independence with increasing scales. (iii) Females of H. tibetana had a strong clonal capacity than male plants at a middle altitude, while the opposite was observed at a low altitude. The number of males of H. tibetana growing at a low altitude and with higher clonal diversity were higher than females at mid altitude. Our findings will contribute to the understanding of the sexual dimorphism exhibited by dioecious plants as well as the importance of a clonal adaptation in response to environmental change. Full article

Background: Research has consistently shown increased drug use among lesbian, gay, bisexual, transgender, intersex, queer/questioning, and asexual (LGBTQIA+) individuals. This is particularly the case among LGBTQIA+ adolescents. Substance use within this vulnerable community can propagate mental health issues, leading to psychiatric disorders, self-harm, and even suicide. Therefore, the objective of this scoping review was to evaluate the determinants of drug use among LGBTQIA+ adolescents. Methods: A comprehensive search of mainly primary research was conducted, using several databases. Peer-reviewed articles published between 2018 and 2023 were included. The scoping review was conducted using the framework outlined by Joanna Briggs Institute (JBI) and reported using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses—Extension for Scoping Reviews (PRISMA-ScR) statement. Results: A total of 29 articles (including 400,194 participants) were included in the analysis. The articles reported that the main determinants of drug use among LGBTQIA+ adolescents include homelessness, peer–peer interactions, mental health, and protective factors. The articles reported that mental health issues, which were mainly triggered by rejection, were the main determinants of drug use among LGBTQIA+ adolescents. Conclusions: Findings from this scoping review provide relatively reliable evidence that homelessness, mental health, peer–peer interactions, and protective factors are the main determinants of illicit drug use among LGBTQ+ adolescents. Rigorous studies including large sample sizes and systematic reviews are needed to further confirm these findings and assist in developing interventions to combat the unusually high level of drug use among this group. Full article