Search Results (6)

Soil salinization threatens crop production; however, in multi-crop field systems, evidence for the effectiveness of waste biomass-functional microorganism composite amendments remains limited. Here, we developed a composite microbial soil conditioner (F2) using pine needles and crushed corn cobs as carriers combined with salt-tolerant strains Bacillus subtilis (K1), Azotobacter chroococcum (Y1), and Bacillus gelatinus (J3) to remediate moderately saline-alkali soil from central Gansu (pH 8.36 ± 0.18; EC 1658 ± 55.24 μS·cm⁻¹). Saturation screening identified an optimal carrier ratio of pine needles:corn cobs = 1:2 and an inoculum ratio of K1:Y1:J3 = 1:2:1. In pot experiments, F2 increased soil organic matter and water-holding capacity, enhanced alkaline phosphatase, urease, and sucrase activities, and significantly reduced soil pH and EC. Maize seedling height and chlorophyll content increased by 53.87% and 38.88%, respectively. Amplicon-based microbiome profiling indicated enrichment of beneficial microbial taxa and strengthened primary metabolic functions under F2. Field validation across five crops (flax, potato, edible sunflower, sorghum, and maize) showed consistent growth and yield-related improvements. Overall, these results demonstrate that the biomass–microbe composite amendment effectively alleviates saline-alkali constraints by jointly improving soil properties, microbial functions, and crop performance. Full article

Since there are no products in the European market labelled as low-FODMAP (low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols), patients with irritable bowel syndrome and non-celiac wheat sensitivity often consume gluten-free products. These naturally contain little FODMAP, but have poorer sensory properties and lower nutritional value. This study aimed to develop sensory attractive crackers with high-fibre and low-FODMAP content. Various gluten-free flours (wholemeal buckwheat and millet, white maize), pumpkin seed meal, chia seeds, flax seeds, rice protein, sweet potato, sourdough, and spices were used to develop nine formulations. Using a nine-point hedonic scale and ranking test, four best-scored products were selected for which descriptive sensory analysis was performed and nutritional value and fructan content were determined. Crackers made from maize and millet flour mixtures (ratio 1:2.5) with sourdough and with chia or flax seed addition were rated highest for overall impression (8.2 and 7.0, respectively). Generally, high-fibre content, hardness, chewiness, dark colour, and bitterness lower the acceptability of crackers, but the addition of spices and sourdough can improve their acceptability and marketability. The crackers could be labelled as “gluten-free”, “low-FODMAP” (<0.12 g/100 g), “naturally high-fibre” (7–10 g/100 g of which 17–23% are soluble), and “high in protein” (24–26 g/100 g). Full article

Biodegradable paper mulch has the advantages of being easily degradable and environmentally benign, but its own performance and adaptability to harsh environments have not been tested. This paper uses scanning electron microscopy and three-dimensional morphometry to microscopically characterize biodegradable paper mulch and white plastic mulch. To analyze and compare their mechanical and hydrophobic properties, and weather resistance, the two mulches were measured through tensile tear load and static contact angle. A comparative analysis of the effect of mulching in the dry crop area of the Hexi Corridor was conducted by comparing the growth index, farm water heat, soil oxygen content, and yield using maize and flax. The test results show that biodegradable paper mulch films were slightly inferior to traditional white mulch films in terms of mechanical and hydrophobic properties, with inadequate insulation and moisture retention, but better in terms of aging resistance, soil oxygen content, and crop insulation and water storage capacity in the middle and growth stages. White mulch film had a better yield enhancement effect on maize, while with biodegradable paper mulch film, this was more significant with flax. Full article

Soil salinization is an environmental problem that adversely affects plant growth and crop productivity worldwide. As an alternative to the conventional approach of breeding salt-tolerant plant cultivars, we explored the use of plant-growth-promoting rhizobacteria (PGPR) from halophytic plants to enhance crop growth under saline conditions. Here, we report the effect of five PGPR consortia from halophytes on the growth of eight (alfalfa, flax, maize, millet, rice, strawberry, sunflower, and wheat) of the crops most commonly produced on salinized soils worldwide. To test the efficiency of halotolerant consortia, we designed a complex environmental matrix simulating future climate-change scenarios, including increased CO₂ levels and temperature. Overall, biofertilizers enhanced growth of most crops with respect to non-inoculated control plants under different CO₂ concentrations (400/700 ppm), temperatures (25/+4 °C), and salinity conditions (0 and 85 mM NaCl). Biofertilizers counteracted the detrimental effect of salinity on crop growth. Specifically, strawberry and rice showed the greatest positive additive response to inoculation in the presence of salt; above-ground biomasses were 35% and 3% greater, respectively, than their respective control grown without salt. Furthermore, depending on the interaction of environmental factors (salinity × CO₂ × temperature) analyzed, the results varied—influencing the most effective biofertilizer determined for each crop now, or in the future. Our findings highlight the importance of conducting studies that consider stress interaction for realistic assessments of the potential of biofertilizers in a climate-changed world. Full article

The practice of crop rotation can significantly impact carbon sequestration potential. In exploring whether crop rotation has the potential to improve soil carbon sequestration in China’s Loess Plateau, soil organic carbon (SOC), soil water content (SWC), soil bulk density (SBD), and soil pH were compared across the 0–1.0 m soil profile, under four crop rotation patterns: lentil–wheat–maize, wheat–potato–lentil, wheat–maize–potato, and wheat–flax–pea. The lentil–wheat–maize and wheat–maize–potato rotations have been practiced over the past 20 years, while the wheat–potato–lentil and wheat–flax–pea rotations were established in 1978 (~40 year rotations). The results showed that under the 20-year lentil–wheat–maize rotation, SOC was not significantly different to that of the wheat–maize–potato rotation, at 6.81 g kg⁻¹ and 6.91 g kg⁻¹, respectively. However, under the lentil–wheat–maize rotation, SWC (9.81%) and SBD (1.19 Mg m⁻³) were significantly higher, but soil pH (8.42) was significantly lower than the same metrics under wheat–maize–potato rotation (8.43% and 1.16 Mg m⁻³, and 8.50, respectively). For the 40-year rotations, SWC (9.19%) and soil pH (8.41) under the wheat–potato–lentil were not significantly different to that of the wheat–flax–pea (8.87%, and 8.40, respectively). SOC (6.06 g kg⁻¹) was significantly lower, but SBD (1.18 Mg m⁻³) was significantly higher under the wheat–potato–lentil than the wheat–flax–pea (7.29 g kg⁻¹, and 1.15 Mg m⁻³, respectively) rotations. Soil carbon sequestration for the lentil–wheat–maize and wheat–potato–lentil rotations was co-influenced by SWC, SBD, and soil pH, while for wheat–maize–potato and wheat–flax–pea rotations, it was co-influenced by SWC and soil pH. The economic value of the four studied crops is, in order: potato > maize > wheat > flax. The results of the present study suggest that the lentil–wheat–maize and maize–flax–pea rotations are the most suitable patterns to optimize simultaneous economic and ecological development of the study area. Full article

Flax (Linum usitatissinum L.) oil is an important source of α-linolenic (C18:3 ω-3). This polyunsaturated fatty acid is well known for its nutritional role in human and animal diets. Understanding storage lipid biosynthesis in developing flax embryos can lead to an increase in seed yield via marker-assisted selection. While a tremendous amount of work has been done on different plant species to highlight their metabolism during embryo development, a comprehensive analysis of metabolic flux in flax is still lacking. In this context, we have utilized in vitro cultured developing embryos of flax and determined net fluxes by performing three complementary parallel labeling experiments with 13C-labeled glucose and glutamine. Metabolic fluxes were estimated by computer-aided modeling of the central metabolic network including 11 cofactors of 118 reactions of the central metabolism and 12 pseudo-fluxes. A focus on lipid storage biosynthesis and the associated pathways was done in comparison with rapeseed, arabidopsis, maize and sunflower embryos. In our hands, glucose was determined to be the main source of carbon in flax embryos, leading to the conversion of phosphoenolpyruvate to pyruvate. The oxidative pentose phosphate pathway (OPPP) was identified as the producer of NADPH for fatty acid biosynthesis. Overall, the use of 13C-metabolic flux analysis provided new insights into the flax embryo metabolic processes involved in storage lipid biosynthesis. The elucidation of the metabolic network of this important crop plant reinforces the relevance of the application of this technique to the analysis of complex plant metabolic systems. Full article