Search Results (646)

The corrosion behavior and time-dependent mechanism of 22MnB5 steel featuring a thinned Al-Si coating (60 g/m²) were systematically investigated in a chloride ion wet–dry cyclic environment, motivated by the demand for thinning and toughening development of aluminum-silicon coatings. A periodic immersion accelerated corrosion test using 3.5% NaCl solution was conducted, together with macro/microscopic morphology observation (SEM/EDS), phase analysis (XRD, FTIR), and electrochemical measurements (polarization curves, EIS). The Al-Si coated steel was studied over corrosion periods of 1, 8, 10, and 20 days to elucidate its corrosion behavior, interfacial evolution, and failure mechanism. The results indicated that the corrosion process exhibited a three-stage evolution: stable protection, rapid failure, and dynamic equilibrium. At the initial stage (1 day), a dense Al₂O₃ passive film formed on the coating surface, providing excellent substrate protection, with a corrosion current density of only 1.77 µA/cm² and a maximum charge-transfer resistance (R₂) of 652 Ω·cm². In the middle stage (8 days), Cl⁻ permeated through the cracked film, triggering selective dissolution of Al, while Si was enriched in situ to form a porous residual layer; the corrosion current density (I_corr) sharply increased to 13.25 µA/cm², and R₂ dropped to its minimum of 156.6 Ω·cm². Corrosion products at this stage were mainly Al₂O₃ and SiO₂, accompanied by small amounts of iron oxyhydroxides and hydroxides, and local coating failure began to appear. During the later stage (10–20 days), the corrosion products evolved into γ-FeOOH, α-FeOOH, and Fe₂O₃, which, together with an amorphous SiO₂ gel network enriched at the interface, formed a dual-layer composite rust layer. R2 consequently recovered from 156.6 Ω·cm² at 8 days to 424 Ω·cm² at 20 days, indicating a reduced corrosion rate and entry into a stable inhibition stage. The critical failure mechanism is that Cl⁻ preferentially penetrates the surface of the Al₂O₃ passive film, disrupting the metastable state of the coating and thereby creating pathways for corrosive media intrusion. The findings of this study can provide technical support for the safe application of such as-received coatings in non-load-bearing components with heat and corrosion resistance requirements. Full article

With the rapid expansion of wind energy infrastructure, there is an increasing accumulation of wind turbine blade waste (WTBW), which is mainly composed of glass fiber-reinforced thermosetting composites. Due to the irreversible nature of polymer crosslinking, conventional recycling methods remain limited. In this study, plasma vitrification was employed to convert WTBW into a reactive calcium-aluminum-silicate slag suitable for use in geopolymer materials. Plasma treatment at a temperature of approximately 2750 K resulted in the formation of predominantly amorphous vitrified slag (VS). Structural characterization using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS) revealed the spatial heterogeneity of the VS. This heterogeneity was influenced by thermal gradients and varied between samples collected from different slag discharge zones, both vertically and horizontally from the reactor. All VS samples contained between 30 and 89% amorphous phase and 10–55% anorthite, with the proportions varying by sampling location. Chemical stability tests showed the dissolution of calcium and aluminum in acidic media, resulting in a silica-enriched residual structure in which the Ca and Al content decreased to less than 0.5 at.% after 100 days. In contrast, exposure to alkaline media caused only minimal surface reorganization—the addition of 5 wt.% VS to acid-based geopolymers made with two metakaolin precursors resulted in a 35% decrease in the mechanical strength of pure metakaolin-based systems. In contrast, when metakaolin containing illite impurities was used, strength values were similar to those of the reference geopolymer. The results quantitatively demonstrate that plasma-derived slag exhibits composition-dependent reactivity, directly linked to its amorphous content and dissolution behavior. Full article

Nematode-trapping fungi are saprophytic organisms that can transform their mycelium into a parasitic lifestyle, forming traps to capture and feed on nematodes. Although this transition is triggered by environmental conditions, the genetic regulation of this metabolic shift remains unclear. This study assessed the effects of nutritional stress on mycochemical synthesis, trap morphogenesis, and Aomae1 gene expression in Arthrobotrys oligospora and Arthrobotrys musiformis. Fungal biomass was subjected to the following three-stage successive culture model: (i) nutrient-rich (Czapek–Dox broth), (ii) nutritional stress (water), and (iii) media enriched with live prey (Haemonchus contortus Hc-L₃). Samples were taken for molecular analysis, and liquid culture filtrates (LCFs) were recovered for chromatographic identification of mycochemical groups. To assess trap formation (traps/cm²), mycelia from each culture model was transferred to water agar plates and defied with Hc-L₃. Results showed a significant bioenergetic trade-off. Both starvation and larval presence induced a downregulation of mycochemical synthesis, which resulted in the total loss of nematocidal activity in LCfs, while triggering a morphogenetic response. Arthrobotrys musiformis showed the most aggressive phenotype with 3.8-fold increase in trap formation and a massive 429.05-fold overexpression of Aomae1 under predatory challenge. While A. oligospora showed a similar but less pronounced trend (2.4-fold increase in trap formation and 44.48-fold Aomae1 overexpression), our findings suggest that Aomae1 expression plays a critical role in the metabolic switch that regulates and redirects energy resources, prioritizing mechanical trapping mechanisms over secondary metabolism during nutrient scarcity. These findings highlight Aomae1 as a possible key activator for virulence, which offers strategic targets for the optimization of biocontrol agents against gastrointestinal nematodes in livestock. Full article

Underground Gas Storage (UGS) is transitioning from traditional fossil fuel peak-shaving facilities into comprehensive hubs for Terawatt-hour-scale Terawatt-hour (TWh) scale renewable energy storage. The unique physicochemical properties of diverse fluids, such as the negative Joule–Thomson coefficient of hydrogen (−0.03 K/bar), present complex engineering adaptability challenges. Since existing studies primarily focus on single mechanisms or specific geological types, this review integrates a unified engineering framework to evaluate the repurposing potential and retrofitting requirements of existing oil and gas assets. By compiling a property benchmarking matrix for methane, carbon dioxide, and hydrogen, the storage adaptability of various geological formations is summarized. Salt caverns exhibit strong adaptability to highly diffusive and reactive fluids due to their high salinity (exceeding 150 g/L) and mechanical stability, whereas porous media offer massive capacity (more than 10 times) but require overcoming severe biogeochemical obstacles. Based on thermo–hydro–mechanical–chemical–biological (THMCB) coupling mechanisms, an integrity evaluation system for artificial wellbore and natural geological barriers is systematically reviewed. Critical risks, including fatigue failure under high-frequency cyclic loading, material degradation, gas leakage, and indirect Global Warming Potential (GWP), are elucidated. A future evolution route integrating physical, digital, and policy dimensions is outlined. This roadmap emphasizes Hydrogen-Enriched Compressed Natural Gas (HCNG)synergistic storage, dynamic risk control utilizing digital twins and Artificial Intelligence (AI), and standardized Life Cycle Assessment mechanisms (LCA), providing a scientific basis for the sustainable transition of UGS facilities. Full article

For the environment and the daily life of urban settlements, in the context of contemporary challenges, severe meteorological events rank second worldwide. Therefore, these events tend to become a real threat to human society and to specific economic activities. The main objective of this study is to analyze the spatio-temporal evolution of severe meteorological events in urban environments and to assess their relationship with atmospheric circulation regimes and urban thermal conditions. The analysis focuses on five types of severe events (significant atmospheric precipitation, hail, strong winds, tornadic structures, and cloud-to-ground lightning) recorded in 11 cities located in the historical region of Muntenia, Romania, over the period 2014–2024. The methodological framework is based on three complementary components. First, a new database was developed by integrating information from multiple sources, including the National Meteorological Administration (ANM), the European Severe Storms Laboratory (ESSL), international databases, and validated media reports, with spatio-temporal filtering and aggregation into synoptic episodes. Second, atmospheric circulation regimes were identified using ECMWF ERA5 reanalysis data, based on geopotential height anomalies at the 500 hPa level, allowing the classification of large-scale synoptic patterns. Third, urban thermal conditions were assessed using the ECMWF CERRA regional reanalysis dataset, which provides high-resolution air temperature data, enabling the analysis of urban–peri-urban thermal contrasts and the estimation of the urban heat island effect. The results highlight a total of 997 severe meteorological events, of which 253 (25.6%) were recorded in the analyzed urban areas, 85 (15.9%) in other towns, and 583 (58.5%) in rural areas. The analysis reveals pronounced interannual and intraseasonal variability, as well as distinct spatial clustering patterns, particularly in urban and peri-urban zones. Among the circulation regimes, the Zonal Regime exhibits the highest event rate, suggesting increased favorability for severe weather occurrence, while other regimes show weaker or even inhibitory effects. In addition, most severe events were associated with positive urban–peri-urban temperature contrasts, indicating an active contribution of the urban heat island effect. By combining observational data, synoptic-scale analysis, and urban-scale thermal assessment, this study provides an integrated regional perspective on severe meteorological events and contributes to the enrichment of data sources in the region, while improving the understanding of their dynamics in urban environments affected by data limitations. Full article

Fungi represent a prolific source of structurally diverse secondary metabolites, yet the extent to which culture conditions reshape the metabolic profile and functional bioactivity remains incompletely understood. In this exploratory study, ten fungal strains belonging to genera Penicillium and Aspergillus were cultivated in Yeast Extract Sucrose (YES) and Czapek Yeast Autolysate (CYA) media and analysed using untargeted LC-HRMS metabolomics. The objective of this study was to evaluate how culture medium influences metabolic profiles and to investigate medium-dependent metabolic variation and its relation to cytotoxic, antibacterial, and antifungal activities. Global metabolic profiling revealed moderate but statistically significant medium-associated metabolite variation, with discriminant metabolites predominantly enriched under CYA conditions. Putative structural annotation suggested patterns consistent with differential regulation of isoprenoid-derived sterols, terpenoids, alkaloid-like metabolites, and aromatic polyketides. While antimicrobial activities displayed a heterogeneous, strain-dependent pattern with limited correlation to individual metabolites, cytotoxic activity co-varied with metabolite composition in OPLS regression modelling. Sterols and terpenoid-related features emerged as major contributors to cytotoxicity. Given the absence of biological replication and the limited sample size inherent to this pilot study, all findings should be considered hypothesis-generating and interpreted within an exploratory framework. These results suggest that nutrient composition influences biosynthetic pathway activation while functional outcomes remain strongly dependent on strain-specific metabolic capacity. This work provides a systematic framework and targeted hypothesis for future investigations into condition-dependent fungal chemical diversity in natural product discovery. Full article

This research focuses on the synthesis of a novel baking powder enriched with bioactive molecules recovered from olive pomace via ultrasound-assisted extraction using a hydro-ethanolic mixture. The functional ingredient was engineered by anchoring the extracted phytocompounds onto a starch backbone through a sustainable grafting technique. Biscuits formulated with the innovative ingredient showed an increased concentration of phenolic compounds (2.162 mg GAE/g), encompassing both phenolic acids (0.372 mg GAE/g) and flavonoids (0.360 mg CTE/g). Enhanced antioxidant efficacy was recorded, mostly in aqueous media (IC₅₀ = 0.554 mg mL⁻¹ against ABTS radical) compared to organic environments (IC₅₀ = 0.132 mg mL⁻¹ against DPPH radical). Furthermore, Oxitest and oxidation stability reactor analyses revealed exceptional antioxidant capacity (induction period = 37 ± 2 h). By an accelerated shelf-life test, a marked instrumental color difference was observed with the fortified sample showing a darker, redder/brown color (ΔE > 16), as also confirmed by trained panelists. On the contrary, similar scores were achieved for the olfactory, textural and tasting attributes of the two samples, as well as values of the friability index (<1 mm⁻¹) evaluated by instrumental techniques. This approach represents a sustainable strategy, transforming a high-polluting agri-food by-product into a source of bioactive compounds for nutritional and technological improvement of baked foods. Full article

Entomopathogenic nematodes (EPNs) are crucial biocontrol agents, yet optimizing the yield and quality of infective juveniles (IJs) during commercial liquid production remains challenging. This study utilized a central composite rotatable design to optimize liquid culture parameters (ascaroside, dimethyl sulfoxide, medium volume, IJ inocula) for Heterorhabditis bacteriophora H06 and Steinernema carpocapsae All. The results demonstrated that improving aeration (inferred from reduced media volume), combined with ascr#11 regulation, synergistically enhanced IJ yield and quality. Under optimized conditions, yields reached 3.35 × 10⁵ IJs/mL for H. bacteriophora H06 and 2.67 × 10⁵ IJs/mL for S. carpocapsae All. Crucially, the IJs from the high-yield flask exhibited significantly superior infectivity (24–26% single-IJ infection rate) compared to solid-culture controls (13–14%). Targeted metabolomics profiling of sugar, energy and fatty acids of H. bacteriophora H06 revealed upregulated tricarboxylic acid (TCA) cycle intermediates (citrate, pyruvate) and the significant accumulation of stress-protectant trehalose and immune-modulating polyunsaturated fatty acids (eicosapentaenoic acid, arachidonic acid). These findings establish a fermentation strategy that simultaneously enhances IJ yield and biological quality by reducing media volume (used as a proxy for improved aeration) and supplementing ascr#11. Furthermore, the distinct metabolic profile enriched in energy, stress, and immune-modulating metabolites identified in H. bacteriophora provides a plausible explanatory framework for the parallel phenotypic improvements observed across both species. Full article

Microorganisms represent the Earth’s most abundant biomass and a vast reservoir of genetic diversity. However, traditional agar plate methods fail to recover the vast majority of these species, leaving a “microbial dark matter” that holds immense potential for the discovery of novel antibiotics and bioactive compounds. While conventional techniques such as selective media and enrichment culture remain foundational, they are inherently limited by community biases and the inability to support low-abundance, oligotrophic species. To address these bottlenecks, a diverse array of innovative isolation strategies has emerged. This review systematically categorizes and evaluates these methodologies, ranging from in situ cultivation to high-resolution single-cell manipulation. We first examine membrane diffusion-based cultivation (e.g., iChip), which mimics natural microenvironments to resuscitate recalcitrant microbes. Subsequently, we explore high-throughput single-cell technologies, including microfluidics for physicochemical separation, optical tweezers for precise manipulation, and fluorescence-activated cell sorting (FACS). Special attention is given to Raman-activated cell sorting (RACS) as a label-free functional screening tool and reverse genomics for targeted capture. By synthesizing the strengths and limitations of these approaches, we propose integrated workflows designed to accelerate the mining of untapped microbial resources. Full article

Background/Objectives: Amniotic-derived biologics have emerged as powerful modulators of tissue regeneration. This study evaluates the composition and characteristics of a human stem cell-conditioned media (hSCCM) that is a sterile, cell-free, amniotic-derived solution, and the presumed efficacy of hSCCM as an active ingredient in an enriched cosmetic lotion. Methods: Data from preclinical benchtop studies and an exploratory observational assessment were reviewed. First, an investigation of the active ingredient, hSCCM, was completed. Flow cytometry assays were completed for mesenchymal stem cell (MSC) characterization. Cellular proliferation assays were conducted to evaluate concentration response, shelf life, and temperature stability. ELISA and LC-MS/MS were used to specify and detail the proteomics of the hSCCM. Second, the hSCCM-enriched lotion’s cosmetic safety and efficacy were evaluated. Preliminary microbial, stability, and early-stage nonclinical retrospective user evaluation of the hSCCM-enriched lotion was conducted to help characterize the cosmetic and evaluate topical safety and efficacy. Results: Flow cytometry demonstrated alignment with ISCT (International Society for Cell and Gene Therapy) characterization for MSCs. Initial in vitro data demonstrated enhanced proliferative effects at hSCCM concentrations as low as 5% (p-value < 0.0001); no statistically significant trend in proliferative capability in aged samples (p-value = 0.79), and no significant effect on proliferative capability when exposed to acute temperature changes (p-values all above 0.05) were observed. Proteomic characterization showed an enriched amniotic-derived solution. Microbial testing of the enriched lotion demonstrated success with multiple unique preservative formulations. hSCCM-enriched lotion demonstrated stability across acute cold- and heat-stress representative scenarios. An exploratory retrospective observational analysis revealed promising trends. Conclusions: The hSCCM demonstrates topical efficacy across in vitro dermal and follicular assays via proliferative and regenerative mechanisms and protein enrichment. The enriched lotion showed success in early-stage microbial and stability testing and demonstrates positive trends in topical skin outcomes. These findings support their potential translational application in dermatologic and aesthetic usage, and broader integumentary contexts. Full article

Aqueous proton batteries (APBs) are promising for safe energy storage, yet their cathode development is hindered by the lack of organic materials with reversible redox activity and long cycling stability in acidic media. Herein, an acceptor-enriched PNZ–TCNQ organic charge-transfer complex was constructed by increasing the TCNQ ratio. Spectroscopic results are consistent with strengthened donor–acceptor interactions and altered local electronic environments. The PNZ–TCNQ cathode delivered ~190 mAh g⁻¹ at 0.6 A g⁻¹ and retained ~85% capacity after 10,000 cycles at 5 A g⁻¹ in acidic three-electrode tests. Kinetic analyses revealed mixed charge storage contributions from pseudocapacitive and diffusion-influenced processes. In situ/ex situ characterizations confirmed reversible redox evolution of the donor–acceptor complex with preserved molecular backbones. This work shows that tuning intermolecular charge-transfer interactions is an effective strategy for improving the cycling stability of organic cathodes in acidic aqueous electrolytes. Full article

In ruminant nutrition, the lignocellulosic complex is a primary constraint limiting the utilization of dietary fiber. The objective of this study was to evaluate the effects of inoculating lignin-degrading bacteria (LDB) isolated from the ruminant rectum on in vitro rumen fermentation characteristics. Rectal fecal samples were collected from healthy beef cattle, dairy cattle, buffaloes, and goats (n = 4 per species) using the grab sampling technique. Twenty-eight bacterial colonies were isolated through enrichment and screening on media containing sodium lignosulfonate. Lignin degradation efficiency was assessed spectrophotometrically, while laccase activity was determined using a 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) oxidation assay. Seven isolates exhibiting ligninolytic activity (1.4–5.6% degradation efficiency) were selected to evaluate their effects on in vitro rumen fermentation using a completely randomized design with four replicates. LDB treatments were standardized to a concentration of 2.4 × 10⁵ colony-forming units/mL of rumen fluid medium, while the control received an equal volume of a 0.85% sterile NaCl solution. A rice straw-based total mixed ration served as the substrate, with rumen fluid collected from beef cattle. All treatments were incubated for 48 h. Notably, isolate BC3 consistently enhanced in vitro dry matter digestibility (23.1%), total gas production (18.6%), and total volatile fatty acid concentrations (13.2%) relative to the control and other LDB isolates (p < 0.01). All seven LDB isolates were identified as Gram-negative, rod-shaped, facultative anaerobic bacteria that exhibit catalase activity and tolerate moderately acidic conditions. Phylogenetic tree analysis based on 16S rRNA gene sequencing identified isolate BC3 as being closely related to Escherichia coli strains. These findings demonstrate that the ruminant hindgut is a promising source of LDB with the functional potential to enhance feed digestibility and fermentation end-products in the rumen. Future research should prioritize in vivo trials to evaluate the safety and efficacy of LDB as a direct-fed microbial, specifically focusing on its impact on animal performance and health. Full article

The conversion of primed pluripotent stem cells to a naive-like state has emerged as a critical strategy for enhancing developmental potential and broadening applications in regenerative medicine. Conditioned media (CM)-based approaches provide a supportive microenvironment enriched with secreted factors that may facilitate this state transition without extensive genetic or chemical manipulation. In this study, we investigated the potential of human Wharton’s Jelly-derived mesenchymal stem cell-conditioned media (hWJ-MSCs-CM) and mouse embryonic fibroblasts CM (MEFs-CM) to support the conversion of primed rhesus monkey embryonic stem cells (rhESCs) into a naive-like state. The rhESCs were cultured under feeder-free and feeder conditions using both hWJ-MSCs-CM and MEFs-CM, exhibiting distinct morphological changes during conversion. Immunofluorescence analysis demonstrated the expression of pluripotency and naive markers under both conditions. Gene expression analysis further confirmed the upregulation of naive-specific genes and downregulation of primed markers, with statistically significant differences between groups. Additionally, epigenetic reprogramming was assessed, revealing differential effects of the CM sources on the reversion to a naive state. These findings highlight the potential of hWJ-MSCs-CM as a supportive system for naive-like state induction in primate ESCs. Full article

Industrial heritage in resource-depleted mining towns faces the dual challenge of physical decay and social severance. To achieve sustainable urban revitalization, adaptive reuse strategies must align with local collective memory and emerging experiential consumption trends. Adopting a Scene Theory perspective, this study constructs a multi-level analytical framework using Meitanba Town (Hunan, China) and its power plant as a case study. A mixed-methods approach was employed, combining semantic network analysis of 1582 online user comments with 61 offline questionnaires distributed to local residents to quantitatively diagnose current scene elements, functions, and features. The quantitative results reveal a significant imbalance: while “Functional Media” achieved the highest comprehensive score (10.0) due to strong historical recognition, “Diverse Groups” scored the lowest (3.4), indicating a lack of social inclusivity. Specifically, residents expressed the highest demand for sports facilities (31.2%) and cultural spaces (23.7%), identifying the main workshop (26.4%) and chimney as core carriers of industrial identity. Responding to these findings, the paper proposes three targeted strategies: (1) Activate: creating open-access recreation scenes to satisfy urgent sports demands; (2) Link: constructing immersive cultural scenes to narrate the “coal–electricity–life” history; and (3) Enhance: developing industry-powered commercial scenes to avoid homogenization. This study enriches the localized application of Scene Theory and provides a data-driven, context-adjustable analytical and strategic model that can inform the sustainable renewal of mining towns globally, with its specific implementation requiring adaptation to local social, economic, and cultural characteristics. Full article

Aggressive behavior in Muscovy ducks (Cairna moschata) has become a predominant concern in intensive farming systems, leading to reduced animal welfare and production losses. To unravel the molecular mechanisms underlying this behavior, transcriptomic profiling was performed on the hypothalamus, a key regulatory hub for aggressive responses. A total of 120 healthy 60-day-old female Muscovy ducks were continuously monitored for 24 h/day over one month using Media Recorder 2.0 software. Based on instantaneous and continuous behavioral observations, the ducks were categorized into three groups: aggressor (Experimental group I, actively attacking conspecifics), victim (Experimental group II, receiving aggression), and non-aggressive (Control group, no aggressive interactions). Hypothalamic tissues were collected from each group (n = 4 per group) for Illumina HiSeq 2000 high-throughput transcriptome sequencing. Functional annotation and enrichment analysis of differentially expressed genes (DEGs) were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, followed by quantitative real-time PCR (qRT-PCR) validation. GO analysis identified 626 DEGs in the aggressor group and 649 DEGs in the victim group compared to the control group, with 26 DEGs directly involved in aggressive behavior regulation. Integration of GO and KEGG annotations revealed 69 candidate genes associated with aggressive behavior, enriched in two GO terms (behavior [GO:0007610] and sensory perception of pain [GO:0019233]) and the ERBB signaling pathway (map04012). qRT-PCR validation of 14 randomly selected candidate genes (e.g., NPY, ERBB4, MAPK9, PRDM12) confirmed that their expression patterns were consistent with transcriptomic data, verifying the reliability of the sequencing results. These findings provide novel insights into the molecular genetic basis of aggressive behavior in Muscovy ducks and lay a foundation for developing targeted strategies to mitigate aggression in intensive farming systems. Full article