Search Results (623)

Food waste is a global challenge, with nearly 40% of food discarded annually, leading to economic losses, food insecurity, and environmental harm. Major factors driving spoilage include microbial contamination, enzymatic activity, oxidation, and excessive ethylene production. Active packaging offers a promising solution by extending shelf life through the selective absorption or release of specific substances. In this study, polyvinyl alcohol (PVA) films incorporating metal-organic frameworks (MOFs) were prepared via solvent casting to enhance their mechanical and barrier properties. Five MOFs (HKUST-1, MIL-88A, BASF-A520, UiO-66, and MOF-801) were embedded in the PVA matrix and analyzed for their physical, mechanical, and optical characteristics. The incorporation of TEMPO-oxidized cellulose nanofibers (CNF) improved MOF dispersion, significantly strengthening film performance. Among the formulations, PVA-CNF-MOF-801 exhibited the best performance, with a 130% increase in tensile strength, a 50% reduction in water vapor permeability, and a 168% improvement in UV protection compared with neat PVA films. Ethylene adsorption tests with climacteric fruits confirmed that CNF-containing films retained ethylene more effectively than those without CNFs, although the differences among the MOFs were minimal. These results highlight the potential of PVA-CNF-MOF composite films as sustainable active packaging materials, providing an effective strategy to reduce food waste and its environmental impact. Full article

Breastfeeding is a complex biological system and a bidirectional physiological dialogue in which the infant may contribute to maternal breast health. This review synthesizes current evidence, clearly separating established findings from emerging hypotheses, to examine the possible infant-driven mechanisms that influence hormonal and immune homeostasis in the mammary gland. We evaluate how neonatal suckling coordinates interconnected hormonal reflexes and immune activity, and we explore the hypothesis that the retrograde flow of infant saliva to the breast tissue could activate maternal enzymatic defenses, particularly the xanthine oxidase and lactoperoxidase systems. We also consider the activation of antimicrobial peptides through direct contact at the nipple and areola, including cathelicidin and defensins, as well as the potential roles of fetal microchimerism and microbial transfer from the infant’s mouth in strengthening breast resilience. Although much of the evidence remains indirect and based on in vitro and animal models, the convergence of data supports a reformulated conceptual model that presents the infant as an active physiological partner rather than a passive recipient of milk. Recognizing this shift has important clinical implications for the prevention of inflammatory conditions such as mastitis, the improvement of breastfeeding support strategies, and the optimization of maternal and infant health outcomes. The review also identifies significant gaps in current knowledge and cautiously proposes hypotheses to explore these mechanisms. While preliminary, this framework offers an original perspective that may guide future research and open new paths in the study of human lactation biology. Full article

The Pulsed Electric Field (PEF) technique represents a modern technology for treating and processing food and agricultural raw materials. The application of high-voltage electric pulses has been shown to modify macrostructure, improve extractability, and enhance the microbial safety of the treated matrix. In this study, we investigated metabolomic changes occurring during the individual technological steps of malting following PEF treatment. Methanolic extracts of technological intermediates of malting barley were analyzed using metabolomic fingerprinting performed with UHPLC-HRMS/MS. For data processing and interpretation, the freely available MS-DIAL—MS-CleanR—MS-Finder software platform was used. The metabolomes of the treated and untreated barley samples revealed significant changes. Tentatively identified PEF-related biomarkers included 1,2-diacylglycerol-3-phosphates, triacylglycerols, linoleic acids and their derivatives, octadecanoids, N-acylserotonins, and very long-chain fatty acids, and probably reflect abiotic stress response. Monitoring of the profiles of selected biomarkers in PEF malting batch indirectly revealed a potential enhancement of enzymatic activity after the PEF treatment. These results contribute to fundamental knowledge regarding the influence of PEF on final malt from a metabolomic perspective. Full article

Challenges significantly hinder the sustainable cultivation of tea chrysanthemum, leading to imbalances in soil nutrients, the accumulation of allelopathic phenolic acids, reduced enzymatic activity, and disruptions in rhizosphere microbial communities. To explore potential mitigation strategies, this study systematically evaluated the integrative effects of exogenous methyl jasmonate (MeJA, 0–400 (μmol L⁻¹)) on both soil environmental parameters and plant growth performance under continuous cropping conditions. The results revealed that treatment with 100 (μmol L⁻¹) MeJA significantly enhanced plant height, canopy width, flower number, and fresh flower weight. Concurrently, it improved soil organic matter content, the available nitrogen levels, and redox stability while increasing the activity of key enzymes, including polyphenol oxidase, urease, and catalase. Notably, this treatment markedly reduced the accumulation of allelopathic phenolic acids, such as p-hydroxybenzoic acid and vanillic acid. High-throughput sequencing further demonstrated that 100 (μmol L⁻¹) MeJA optimized the composition of soil microbial communities, increasing the abundance of beneficial taxa, such as nitrogen-fixing and phosphate-solubilizing bacteria, while suppressing pathogenic fungi. Metabolomic analysis showed that this concentration of MeJA activated stress-resistance metabolic pathways involving flavonoids and terpenoids while downregulating degradation-related processes, thereby supporting enhanced plant resilience at the metabolic level. Collectively, these findings demonstrate that an appropriate concentration of exogenous MeJA can effectively alleviate continuous cropping obstacles in Chrysanthemum morifolium, providing both theoretical insights and practical guidance for its eco-friendly and efficient cultivation. Full article

Jujube (Ziziphus jujuba Mill.) is a nutritionally rich and economically significant fruit, highly valuable for its flavor, bioactive compounds, and therapeutic properties. However, it is highly perishable and has a short postharvest lifespan. This review aims to provide knowledge for preserving quality and improving postharvest storage by integrative strategies aimed at extending the shelf life of jujube. The literature was collected from recent peer-reviewed studies on postharvest physiology and handling technologies of jujube fruit. Key physiological factors, influencing postharvest deterioration such as water loss, softening, browning, and decay, are discussed, along with the underlying biochemical and enzymatic mechanisms driving quality decline. Conventional strategies such as cold storage, MAP, and CA effectively slow respiration, delay reddening, and extend storage up to 2–4 months, while emerging approaches such as ozone and cold plasma treatments reduce microbial decay and maintain antioxidant activity. Edible coatings like chitosan, aloe vera, and composites cut weight loss by 20–40%, and chemical regulators such as 1-MCP and calcium dips further delay ripening, preserve firmness, and enhance postharvest quality. Emphasis is placed on integrating innovative technologies with physiological insights to optimize storage conditions, control microbial contamination, and maintain nutritional integrity. The significance of this review lies in integrating physiological insights with innovative preservation methods, offering practical guidance for researchers, growers, and industry stakeholders to achieve sustainable, safe, and market-oriented solutions for jujube storage. Full article

The increasing environmental presence of metal oxide nanoparticles (NMOPs) raises concerns regarding their influence on biological wastewater treatment. This study comparatively evaluates the structural and biological effects of zinc oxide (ZnO-NPs) and titanium dioxide (TiO₂-NPs) nanoparticles on activated sludge from a wastewater treatment plant. Experimental exposure covered nanoparticle concentrations of 0.05–0.3 g/L and contact times up to 180 min, with analysis of enzymatic activity (dehydrogenase activity, TTC-SA method), sludge settleability, and particle size distribution. Inhibition of microbial metabolic activity was observed in a clear dose- and time-dependent manner, with ZnO-NPs showing stronger toxicity than TiO₂-NPs. At the highest dose (0.3 g/L), enzymatic activity nearly disappeared after 90 min (0.04 µg TPF/mg MLSS). Both nanoparticles caused floc fragmentation, decreased sludge volume index (SVI), and increased the proportion of ultrafine particles (<0.3 µm). ZnO-NPs induced more severe destabilization, while TiO₂-NPs showed partial re-aggregation of suspended particles at higher concentrations. Additionally, particle size distribution in the supernatant was analyzed, revealing distinct aggregation and fragmentation patterns for ZnO- and TiO₂-NPs. These structural and functional alterations suggest potential risks for treatment efficiency, including reduced nutrient removal and impaired sludge settleability. The study provides a comparative contribution to understanding toxicity mechanisms of ZnO- and TiO₂-NPs and emphasizes the need to monitor NMOPs in wastewater and to develop mitigation strategies to ensure stable plant operation Full article

This study details a biorefinery approach to valorize Dosidicus gigas squid pen waste. The process starts with the enzymatic deproteinization of squid pens, which prove effective with both Alcalase and Novozym, with the latter exhibiting a slightly higher efficiency to yield a material with 73% chitin content. Subsequent alkaline hydrolysis produces highly deacetylated chitosan (>90% degree of deacetylation), followed by controlled depolymerization to obtain polymers with molecular weights ranging from 50 to 251 kDa. Both native and depolymerized chitosan exhibit antimicrobial activity against Escherichia coli and Bacillus cereus, with B. cereus demonstrating greater resistance to chitosan compared to E. coli. The research also explores the bioconversion of deproteinization and deacetylation effluents. Deproteinization effluents prove superior in sustaining microbial growth, supporting comparable growth and lactic acid production for human probiotic strains (Lactobacillus plantarum and Leuconostoc mesenteroides) when substituting commercial peptones. Marine bacteria (Pseudomonas fluorescens and Phaeobacter sp.) show lower productivity. Integrating these processes into a biorefinery framework enables the conversion of 1 kg of dry squid pens into 350 g of chitosan, and facilitates the production of 937–949 g of lactic acid using human lactic acid bacteria cultures in media formulated with squid pen-derived effluents, glucose, yeast extract, and mineral salts. This integrated approach highlights the potential for maximizing resource utilization from squid pen waste, reducing environmental impact and generating high-value bioproducts. Full article

Micro- and nanoplastics (MPs and NPs) are recognized as emerging contaminants posing potential risks to human health. Recent evidence highlights the potential of food-grade microbial strains to bind these particles and facilitate their removal, suggesting a promising probiotic-based strategy for mitigating their adverse health effects. This study investigated the adsorption and biodegradation capabilities of Bacillus subtilis DCP04, a strain isolated from Korean fermented soybean paste, cheonggukjang, on low-density polyethylene (LDPE) particles. Biofilm formation assays and morphological observations confirmed the strain’s ability to adhere to the surface of LDPE. Subsequent experiments demonstrated that DCP04 effectively adsorbed LDPE particles in a size-, time-, and concentration-dependent manner. This interaction induced significant morphological changes and increased hydrophilicity on the polymer surface. Furthermore, a positive correlation was observed between the activities of laccase and manganese peroxidase and a measurable weight loss in LDPE films, suggesting direct enzymatic involvement in polymer degradation. Crucially, the DCP04 strain also met key safety and functional criteria for use as a probiotic. These findings highlight the potential of DCP04 strain as a functional probiotic agent for mitigating the accumulation of MPs and NPs within the human body. Full article

Alpine wetlands, key carbon sinks and biodiversity hubs, remain understudied, especially under climate change pressures. Hence, the present study was conducted to assess the variability in soil enzyme activity (SEA) and the carbon management index (CMI) and to utilize principal component analysis (PCA) to explore the variation and correlation between SEA and CMI as influenced by altitudinal gradients in alpine wetlands. This information is essential for exploring the impacts of soil degradation and guiding restoration efforts. The study was designed in blocks (catchments) with six altitudinal variations (from 2500 to 3155 m a.s.l), equivalent to alpine wetlands from three catchments (Senqunyane, Khubelu and Sani) as follows: Khorong and Tenesolo in Senqunyane; Khamoqana and Khalong-la-Lichelete in Sani; and Lets’eng-la-Likhama and Koting-Sa-ha Ramosetsana in Khubelu. The soil samples were collected in February 2025 (autumn season, i.e., wet season) at depths of 0–15 and 15–30 cm and analyzed for bulk density, texture, pH, electrical conductivity (EC), soil organic carbon (SOC), SEA, and carbon pools, and the CMI was computed following standard procedures. The results demonstrated that the soil was loam to sandy loam and was slightly acidic and non-saline in nature in the 0–15 cm layer across the wetlands. The significant decreases in SEA were 45.33%, 32.20% and 15.11% (p < 0.05) for dehydrogenase, fluorescein di-acetate and β-Galactosidase activities, respectively, in KSHM compared with those in Khorong (lower elevated site). The passive carbon pool (C_PSV) was dominant over the active carbon pool (C_ACT) and contributed 76–79% of the SOC to the total organic carbon, with a higher C_PSV (79%) observed at KSHM. The CMI was also greater (91.05 and 75.88) under KSHM at the 0–15 cm and 15–30 cm soil depths, respectively, than in all the other alpine wetlands, suggesting better carbon management at higher altitudinal gradients and less enzymatic activity. These trends shape climate change outcomes by affecting soil carbon storage, with high-altitude regions serving as significant, though relatively less active, carbon reservoirs. The PCA-Biplot graph revealed a negative correlation between the CMI and SEA, and these variables drove more variation across sites, highlighting a complex interaction influenced by higher altitude with its multiple ecological drivers, such as temperature variation, nutrient dynamics, and shifts in microbial communities. Further studies on metagenomics in alpine soils are needed to uncover altitude-driven microbial adaptations and their role in carbon dynamics. Full article

Intercropping, especially legume-cereal systems, is a mixed farming approach that can improve agricultural resilience by addressing challenges such as soil degradation, biodiversity loss, and global change, all while promoting the sustainable production of protein-rich and nutritious food. However, its adoption in industrialized countries remains limited due to economic and technical challenges, as well as a fragmented understanding of soil–plant-microbe interactions, which hinders its complete optimization. This article provides an overview of the current situation and future perspectives on the importance of legume–cereal intercropping, with examples such as common bean–maize, soybean–maize, alfalfa–corn–rye, and legumes–pulses–little millet systems. These combinations highlight how intercropping can improve nutrient cycling, increase root growth, forage and grain yield, suppress soil-borne diseases, and promote soil microbial population and enzymatic activity. While it offers environmental benefits, practical challenges such as system design, management complexity, and cost-effectiveness must be addressed to encourage wider adoption. In preparing this review, we synthesized studies published between 2000 and 2025, with a particular emphasis on recent research from China and Southeast Asia. We also considered broader intercropping contexts, including energy crops, agroforestry systems, rice paddy co-cultures, and phytoremediation approaches. The review also highlights legume–cereal as a solution to sustainable soil management, ecosystem health, and the potential for increased nutritional food production in developed countries. Full article

Substituting chemical fertilizers with organic alternatives represents an effective strategy for mitigating soil nitrogen (N) loss and reducing chemical fertilizer use. However, the efficacy of organic substitution in regulating soil N fertility and rice growth requires further investigation, and mechanistic studies elucidating how organic fertilizers affect soil N transformation processes and availability are still deficient. To address this, we conducted a three-year field experiment from 2021 to 2023, comparing three rice fertilization regimes: (1) chemical fertilizer as the control (CK), (2) substitution with organic fertilizer (OF), and (3) substitution with biochar-based organic fertilizer (BF). Both organic substitution treatments were applied as basal fertilizer, and the rice plants received equivalent topdressing applications. The soil N availability, gross and net N transformation rates, and soil microbial activity were analyzed, and the rice growth index and yield were determined. The results showed that organic substitution (OF and BF) significantly increased the soil total carbon content, stimulated microbial biomass growth and enhanced enzymatic activity associated with soil C and N cycling. However, the limited N input from organic substitution significantly decreased the soil gross N mineralization rate by 28.30% (OF) and 58.14% (BF), compared to chemical fertilization (CK). It also reduced the gross N nitrification rate by 38.30% (OF) and 36.17% (BF). These suppressed N transformation processes ultimately led to 11.97% (OF) and 14.72% (BF) lower soil mineral N contents. The soil N deficiency during critical early vegetative growth stages substantially constrained rice development, resulting in significant yield reductions in the OF and BF treatments compared to chemical fertilization (CK). These results indicate that complete organic substitution compromises rice yields due to insufficient N availability; therefore, we recommend integrated organic–mineral fertilization as an optimal strategy to achieve both crop productivity and environmental benefits. Full article

Sustainable fertilization strategies are critical under climate change and the European Green Deal, particularly for Mediterranean cereal systems. Organic fertilizers derived from agro-industrial residues offer promising alternatives to conventional mineral inputs. This study evaluated RecOrgFert, a novel fertilizer composed of sulfur–bentonite and citrus-processing residues, in comparison with NPK (15-15-15) and horse manure across two years in Central Macedonia (Greece) and Apulia (Italy). Using a randomized complete block design, soil chemical and biological properties, plant growth, yield, and grain quality were assessed. RecOrgFert outperformed conventional fertilizers by enhancing soil fertility—raising organic matter 25–27% above control and further increasing it from 2023 to 2024 (up to +75% in Italy, +38% in Greece)—while improving cation exchange capacity, enzymatic activity, and soil water content. Wheat grown with RecOrgFert showed higher protein (up to 15.2%), antioxidant activity (DPPH > 37%, ABTS⁺ > 26%), and phenolic and flavonoid content, with yields comparable to NPK. The unique sulfur and orange-residue composition distinguish RecOrgFert from standard fertilizers, promoting nutrient cycling, microbial activity, and bioactive compound accumulation. It represents a novel, circular, and climate-smart solution aligned with EU sustainability and circular economy objectives. Full article

Nong-flavor (NF) Daqu, a critical fermentation starter for traditional Baijiu, harbors diverse starch-degrading enzymes with poorly characterized functional dynamics. This study transcended traditional quality assessments by developing molecular approaches to dissect starch-hydrolyzing enzyme genes. Specific and degenerate primers targeting glucoamylase, α-amylase, and α-glucosidase genes were designed, and key genes were qualitatively identified with distinct distributions among NF Daqus and unique presences between JXL and HB Daqu. Quantitative PCR revealed six genes with elevated expression in JXL Daqu versus HB Daqu, and which peaked during late fermentation in both Daqus. Metagenomics identified greater enzymatic diversity in HB Daqu. Phylogenetic clustering confirmed evolutionary conservation (GH13/GH15/GH31 families) and specificity of core enzyme genes across both Daqus. Enzymatic assays demonstrated the dominance of saccharification over α-glucosidase activity in both Daqus, with significantly higher α-glucosidase activity in JXL than HB Daqu. Divergent starch degradation strategies emerged: JXL prioritized high enzyme expression/activity, while HB utilized broader gene abundance. Based on Pearson correlation analysis, the saccharification activity showed the highest but weak correlation with α-glucosidase gene_15963 (r = 0.26), and was also positively correlated with the expression of all other enzyme genes except one glucoamylase gene. Meanwhile, α-glucosidase activity was most strongly linked to glucoamylase gene_22243 (r = 0.76), with additional correlations with two α-glucosidase genes being observed. This establishes RNA-based biomarkers for real-time quality control. Our findings decode divergent microbial strategies (JXL: high-expression/high-activity vs. HB: high-diversity) and provide a molecular framework for optimizing starch utilization in Baijiu fermentation. This technology holds potential to enable precision-driven standardization of traditional food production, which would reduce processing waste and enhance resource efficiency. Full article

Saline–alkaline wetlands represent critical ecosystems for maintaining biodiversity, regulating hydrological processes, and supporting regional ecological resilience. However, the extent to which dominant vegetation regulates soil functionality and microbial assemblages in these unique saline systems remains insufficiently understood. In this study, we examined five characteristic vegetation types—Phragmites communis Trin., Typha angustifolia L., Bryophytes, Suaeda salsa (L.) Pall., Echinochloa phyllopogon (Stapf) Koss.—across the saline wetlands of Chagan Lake, northeast China, which are embedded in a heterogeneous matrix of forests, grasslands, and agricultural lands. Comprehensive assessments of soil physicochemical properties, enzyme activities, and bacterial communities were conducted, integrating high-throughput sequencing with multivariate statistical analyses. Our results revealed that vegetation cover markedly influenced soil attributes, particularly total organic carbon (TOC) and alkali-hydrolyzed nitrogen (AN), alongside key enzymatic functions such as urease and alkaline phosphatase activities. Proteobacteria, Actinobacteria, and Acidobacteria emerged as dominant bacterial phyla, with their relative abundances tightly linked to vegetation-induced shifts in soil environments. Notably, soils under E. phyllopogon demonstrated elevated bacterial diversity and enzymatic activities, underscoring the synergistic effects of plant selection on soil biogeochemical health. Structural equation modeling further elucidated complex pathways connecting vegetation, microbial diversity, soil quality, and enzymatic functioning. These findings emphasize the pivotal role of vegetation management in improving soil fertility, shaping microbial communities, and guiding the sustainable restoration of saline–alkaline wetlands under environmental stress. Full article

Background: Sustainable, dual-action ergogenic strategies are underexplored; most products target a single pathway and rarely upcycle seafood sidestreams. We therefore tested an upcycled formulation combining grouper bone hydrolysate and Undaria pinnatifida extract (GU) for ergogenic and microbiota effects in mice. We tested the ergogenic and microbiota modulating effects of GU in mice versus a vehicle and a BCAA control. Methods: GU was prepared via enzymatic hydrolysis of marine by-products and administered to male ICR mice for 4 weeks. Mice were divided into five groups (n = 7/group), receiving a vehicle control, a branched-chain amino acid (BCAA) supplement, or GU at three dose levels (1X, 2X, 3X) based on human-equivalent conversion. Exercise performance was assessed via grip strength and treadmill tests. Biochemical markers of fatigue, body composition, and safety indicators were also analyzed. Gut microbiota was evaluated using 16S rRNA sequencing and constrained principal coordinates analysis (CPCoA). Results: Four weeks of GU supplementation significantly enhanced exercise performance [(treadmill time ↑ Δ = 10.2–11.7 min versus vehicle (q ≤ 0.0002), grip strength ↑ Δ = 40.4–48.5 g (q ≤ 0.05)] and lean body mass [FFM ↑ at GU-1X (Δ = +0.80%, q = 0.0123)], surpassing the commercial BCAA control. Biochemical analyses indicated reduced exercise-induced lactate accumulation [(post-exercise lactate ↓ Δ = −2.71/−2.18 mmol·L⁻¹, q = 0.0006)]. Gut microbiota profiling revealed distinct shifts in community composition in GU-treated groups, notably with an increased abundance of beneficial taxa such as Lactobacillus and Muribaculum. These alterations reflect the prebiotic activity of seaweed-derived polysaccharides, promoting a healthier gut microbial profile. Notably, GU improved metabolic markers (aspartate aminotransferase, [AST]; lactate dehydrogenase, [LDH]) without inducing toxicity. Conclusions: These findings indicate that GU functions as a dual-action supplement, coupling amino acid-mediated muscle anabolism with microbiome modulation to enhance physical performance and metabolic health. As an upcycled marine product, it presents a sustainable and effective strategy for exercise support. Future studies should include 90-day safety, mechanistic assays, and a preregistered human pilot. Full article