Search Results (5,554)

This study employs hydrophobic modification of the current collector to optimize cathode water management and enhance the performance of passive DMFCs. The surface of the cathode current collector was hydrophobized by polytetrafluoroethylene (PTFE) coating and titanium dioxide/polydimethylsiloxane (PDMS) composite coating. The experimental results showed that the surface hydrophobic treatment significantly improved the cell performance at low methanol concentration and marginally improved the cell performance at high methanol concentration. Among them, the DMFC with bilayer TiO₂/PDMS hydrophobic-treated cathode current collector with a contact angle of 153.2° showed the best performance, which achieved superhydrophobicity and led to a peak power density that was 27.25% higher compared to the DMFC with an untreated current collector. With the gradient-based hydrophobic treatment for the cathode current collector, the best performance was achieved when double-layer TiO₂/PDMS was used on the MEA side and PTFE coating on the air side. Full article

Geological data of the region indicate that the Canary Islands have not been connected to the mainland before. However, fossil evidence suggests some kind of faunal exchange with Africa during the late Neogene. After extensive field work during past years, a re-evaluation of the fossil remains of the first terrestrial vertebrates that settled and thrived on the Canary Islands is presented, with special attention to the long-debated identity of birds that laid large-sized eggs, reported some decades ago on Lanzarote Island. The age of the eggshell-bearing deposits has been recently updated as Early Pliocene (ca. 4 Ma). The dispersal mode of these terrestrial birds to reach the island was an unsolvable challenge in previous studies because the regional geography of the sea bottom was neglected, as well as the chronological succession of events in the formation of the Canary Eastern Ridge, which increased attention to a unique case of arrival of ratites on an island never before united with the mainland. The few animals found in northern Lanzarote (ratites, snakes, turtles, terrestrial snails and bite marks on eggshells pointing to a jagged and unknown large predator) probably made the sea crossing from the mainland in different ways. Two scenarios are contemplated. In both, the circumstances facilitating the faunal transit from Africa to the Canaries ceased after the early Pliocene, around 4 Ma, since these animals have never managed to cross the Canary Channel again. Full article

Background/Objectives: Toxoplasma gondii is a major cause of zoonotic infections in both humans and animals, resulting in significant mortality in susceptible species, such as New World primates and marsupials. Toxoplasmosis is particularly concerning in zoos and wildlife reserves, where outbreaks threaten conservation efforts for endangered species. In the absence of a commercially available vaccine against toxoplasmosis for humans and captive wild animals, current prevention strategies are limited to restricting the access of cats to enclosures, controlling rodent populations, and maintaining strict food hygiene. Recent research has shown promising results with an intranasal vaccine (VXN-Toxo) composed of maltodextrin nanoparticles conjugated with a purified, inactivated T. gondii parasite. This experimental vaccine does not pose a risk of causing disease and offers advantages such as better stability compared with live pathogen-based vaccines. Methods: This study presents a large-scale evaluation of the effect of VXN-Toxo administered to captive wildlife across 20 zoos in Europe and the Americas between 2017 and 2025. Seven hundred and eighty-four animals, representing over 58 species (including primates, marsupials, rodents, and felids), were vaccinated without any adverse events reported. Results: Retrospective mortality data from 20 participating zoological institutions revealed an overall 96.7% reduction—and, in many cases, a complete elimination—of toxoplasmosis-associated deaths post vaccination. Conclusions: These results demonstrate, for the first time, consistent and broad-spectrum protection against T. gondii of different strains in a wide array of captive wildlife species. This universal vaccine represents a promising tool for toxoplasmosis prevention in zoological collections, with significant implications for animal health and conservation strategies. Full article

This article analyzes responses of museums and art institutions in Israel to the events of October 7th. It stresses the public role of museums in times of crisis, and the ways that diverse curatorial choices reflect upon their institutions’ pursuits. It focuses on the case study of curatorial empathy, enacted at the Tel Aviv University Art Gallery, noting its aptness at times of crisis and trauma. The article claims that in a society that experiences both internal and external conflicts, the approach of empathy offers flexibility and openness that allow the museum to respond to public need on the one hand, and poses challenging questions on the other. Such questions are explored through the method of artistic-scientific dialogue. As contentions multiply, overlap, and contrast, the expansion of circles of identification becomes a key strategy in addressing this crisis. This essay argues that empathy is a more thoughtful and productive curatorial approach, because it emphasizes connection rather than only identity. From this perspective, the crisis that started on October 7th is not only that of war, loss, and grief, but also that of a threat to humanness under extreme angst. Full article

This study designed and empirically evaluated a method to enhance information accessibility for museum and art gallery visitors using a small Large Language Model (sLLM) based on the Retrieval-Augmented Generation (RAG) framework. Over 199,000 exhibition descriptions were collected and refined, and a question-answering dataset consisting of 102,000 pairs reflecting user personas was constructed to develop DocentGemma, a domain-optimized language model. This model was fine-tuned through Low-Rank Adaptation (LoRA) based on Google’s Gemma2-9B and integrated with FAISS and OpenSearch-based document retrieval systems within the LangChain framework. Performance evaluation was conducted using a dedicated Q&A benchmark for the docent domain, comparing the model against five commercial and open-source LLMs (including GPT-3.5 Turbo, LLaMA3.3-70B, and Gemma2-9B). DocentGemma achieved an accuracy of 85.55% and a perplexity of 3.78, demonstrating competitive performance in language generation and response accuracy within the domain-specific context. To enhance retrieval relevance, a Spatio-Contextual Retriever (SC-Retriever) was introduced, which combines semantic similarity and spatial proximity based on the user’s query and location. An ablation study confirmed that integrating both modalities improved retrieval quality, with the SC-Retriever achieving a recall@1 of 53.45% and a Mean Reciprocal Rank (MRR) of 68.12, representing a 17.5 20% gain in search accuracy compared to baseline models such as GTE and SpatialNN. System performance was further validated through field deployment at three major exhibition venues in Seoul (the Seoul History Museum, the Hwan-ki Museum, and the Hanseong Baekje Museum). A user test involving 110 participants indicated high response credibility and an average satisfaction score of 4.24. To ensure accessibility, the system supports various output formats, including multilingual speech and subtitles. This work illustrates a practical application of integrating LLM-based conversational capabilities into traditional docent services and suggests potential for further development toward location-aware interactive systems and AI-driven cultural content services. Full article

Perylenequinonoid compounds, represented by photosensitive therapeutic agents such as hypocrellins and elsinochromes, demonstrate extensive potential across biomedical, agricultural, and food industrial applications. Nevertheless, their restricted biosynthesis remains a critical bottleneck for commercial exploitation. This study implemented UV mutagenesis to enhance perylenequinone production in fungal strains of Pseudoshiraia conidialis, achieving significant yield improvements at the 120 J/m² and 150 J/m² irradiation intensities. Through systematic optimization of the HPLC analytical platform, we established the precise quantification of five distinct perylenequinonoid derivatives: hypocrellin A, hypocrellin B, shiraiachrome A, elsinochrome A, and elsinochrome B. The mutant strain Z2-1 demonstrated a remarkable biosynthetic capacity with the total perylenequinonoid yields reaching 2101.6 mg/L, representing a 705.70% enhancement over the parental strain zzz816 (260.84 mg/L). Particularly noteworthy was the hyperproduction of hypocrellin A at 1100.7 mg/L, corresponding to a 1208.02% increase from the baseline yield (84.15 mg/L). Furthermore, this work reports the first successful generation of an elsinochrome A-overproducing strain, achieving a 312.68 mg/L output (429.25% increase from 59.08 mg/L). Intriguingly, different mutant strains exhibited distinct production profiles for specific compounds, revealing biosynthetic preference variations among derivatives. These findings emphasize the necessity for comprehensive metabolite profiling during fermentation process optimization to maximize the target compound yields. Full article

Quercus petraea subsp. pinnatiloba is a narrowly distributed oak taxon in southeastern Türkiye, and its taxonomic position has long remained uncertain. This study aims to clarify its distinctiveness by integrating morphological, molecular, and biogeographical evidence. Principal Component Analysis (PCA) and Stepwise Discriminant Analysis (SDA) of 14 leaf traits revealed that subsp. pinnatiloba constitutes a morphologically stable and distinctly differentiated group from other Q. petraea subspecies and closely related taxa, characterized by key diagnostic traits such as petiole length (PL), lamina length (LL), length of leaf blade at its broadest point (WP), and lobe width at the tip of the widest lobe (LW). Phylogenetic analyses based on nuclear ITS and plastid markers (rbcL, psbA-trnH) confirmed its placement within sect. Quercus, yet consistently distinguished it genetically from other subspecies for the first time. Molecular dating (BEAST) suggested divergence in the Miocene (11 Mya with 95% HPD 3.01, 20.95) while RASP biogeographical analysis indicated an origin in the Euro-Siberian region with later dispersal into the Mediterranean. These integrative results support its recognition at species rank as Quercus pinnatiloba, clarifying its phylogenetic placement and underscoring the conservation importance of this lineage. Full article

The aim of this study is to identify the textile materials used to make Bulgarian folk costumes and to support the process of conservation and restoration of ethnographic objects. In the 18th and 19th centuries, folk costumes were made almost exclusively of natural materials, while in the first half and middle of the 20th century, they included contemporary synthetic and regenerated cellulose materials, as well as blends of these materials with natural fibers. A series of historical textiles and contemporary industrial fabrics were studied using a variety of analytical approaches, including micro-chemical staining and solubility tests, optical microscopy, scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDS), and infrared (IR) spectroscopy. IR measurements were carried out in attenuated total reflectance (ATR) and external reflectance (ER) modes using a portable IR spectrometer, which enabled non-invasive analysis. The analysis revealed that the composition of the industrial fabrics and historical textiles encompassed synthetic fibers, such as polyester and polyamide, while others were made of regenerated cellulose fibers like viscose. Additionally, some textiles had a mixed composition of cotton and polyester or silk and viscose. The combined analytical approach provided reliable identification of both the synthetic and natural textile materials. Full article

In the context of the progressive tendency to perceive a degraded environmental state as normal, due to the loss of memory of past ecological conditions (i.e., the Shifting Baseline Syndrome), natural history museum collections represent invaluable resources for studying long-term biodiversity shifts. This study deals with the taxonomic validation of the chondrichthyan species from the historical ichthyological collection assembled by Pietro Doderlein from 1863 to 1922 at the Museum of Zoology of the University of Palermo. The chondrichthyan specimens were digitally catalogued to meet current standards of museum documental identification. Biometric measurements were taken for each specimen, and an integrated analytical approach—combining morphology and ancient DNA analysis—was applied to assign species identities. The collection comprises 342 specimens associated with 76 valid codes. Of these, 288 specimens were identified to species level by morphology, revealing 58 discrepancies with the historical identifications. Sixteen specimens that could not be morphologically assigned were analyzed by DNA barcoding, resulting in eight additional species-level identifications. In total, 62 valid species belonging to 27 families were digitally catalogued according to ministerial guidelines. This taxonomic validation and cataloguing of the “P. Doderlein” chondrichthyan collection represent the first successful effort to bridge the gap in available data and tissue resources from Italian historical natural museums. Full article

This paper provides a comprehensive review of sessile benthic invertebrate biodiversity across three interconnected marine areas in Western Australia’s Gascoyne and southern Pilbara regions: Ningaloo Reef (a UNESCO World Heritage site), the nearby Muiron Islands to its north, and Exmouth Gulf to its east. The study aims to identify taxonomic and spatial knowledge gaps and establish a baseline biodiversity record for the broader region. We collated specimen-based records from seven Australian museums and supplemented them with data from the Atlas of Living Australia, iNaturalist, and a Web of Science literature review. In total, we report 827 verified species of sessile benthic invertebrates in shallow waters (less than 30 m depth) across the Gascoyne and southern Pilbara regions from 4578 records. Ningaloo Reef exhibits the highest diversity, hosting 587 species, including 239 scleractinian corals and 124 sponges. Exmouth Gulf is home to a diverse and unique assemblage of at least 295 species, including 165 species of putative turbid water specialists or regionally restricted species which are not found at Ningaloo Reef or the Muiron Islands. Notably, all three sea pen species identified in this review are recorded exclusively from Exmouth Gulf. The Gulf also hosts a distinctive community of filter-feeding invertebrates, including 125 sponges, 27 tunicates, and 18 anemones that are not found in neighboring regions. The Muiron Islands, although under sampled, host 22 species of sponges, 14 octocorals, and 5 tunicates that have not so far been found at Ningaloo Reef or Exmouth Gulf, highlighting the uniqueness of the islands’ marine invertebrate fauna. The southern sector of Ningaloo Reef is under sampled, and there are gaps in the state faunal collections for many groups, including anemones, corallimorphs, zoanthids, cerianthids, and hydrozoans. Further targeted taxonomic research on existing museum collections is crucial, as only 18% of specimen records have been verified, and 41% of the verified records are only identified to morphospecies. This limits our understanding of regional diversity and distribution patterns but provides ample opportunity for further taxonomic study. Expanding monitoring programs to include Exmouth Gulf and the Muiron Islands, along with unique habitats such as the King Reef artificial reef, and conducting additional biodiversity, demographic, and ecosystem health research is essential to assess the cumulative impacts of climate change and other environmental stressors on the unique and culturally significant marine ecosystems of this region. Full article

Proceroplatus (Keroplatidae: Keroplatinae) is a distinct keroplatid group comprising 39 described species. These species are found worldwide, but none had previously been reported in China prior to this study. In this paper, Proceroplatus is recorded for the first time from China, along with two new species: P. dapanshanussp. n. and P. biemarginatussp. n., which were collected from the southern region. Here, a worldwide distribution map of this genus is presented by species, including the new ones. Images and detailed morphological descriptions are provided for each new species, accompanied by molecular identification based on the standard mitochondrial cytochrome oxidase subunit I (COI) gene. To clarify the mitogenomic characteristics of Proceroplatus, the well-assembled and annotated mitogenome of P. dapanshanus was obtained and described in detail. The comparative analyses and phylogenetic tree indicate that the mitogenomic evolution of keroplatids is relatively conserved and influenced not only by mutation pressure but also by natural selection and other factors. Full article

In response to the urgent global call for “dual carbon” targets, the sustainable transformation of public museums has become a focal issue in both academic research and engineering practice. This study proposes and empirically validates an integrated management framework that unites digital twin modeling, artificial intelligence, and green energy systems for next-generation green smart museums. A unified, closed-loop platform for data-driven, adaptive management is implemented and statistically validated across distinct deployment scenarios. Empirical evaluation is conducted through the comparative analysis of three representative museum cases in China, each characterized by a distinct integration pathway: (A) advanced digital twin and AI management with moderate green energy adoption; (B) large-scale renewable energy integration with basic AI and digitalization; and (C) the comprehensive integration of all three dimensions. Multi-dimensional data on energy consumption, carbon emissions, equipment reliability, and visitor satisfaction are collected and analyzed using quantitative statistical techniques and performance indicator benchmarking. The results reveal that the holistic “triple synergy” approach in Case C delivers the most balanced and significant gains, achieving up to 36.7% reductions in energy use and 41.5% in carbon emissions, alongside the highest improvements in operational reliability and visitor satisfaction. In contrast, single-focus strategies show domain-specific advantages but also trade-offs—for example, Case B achieved high energy and carbon savings but relatively limited visitor satisfaction gains. These findings highlight that only coordinated, multi-technology integration can optimize performance across both environmental and experiential dimensions. The proposed framework provides both a theoretical foundation and practical roadmap for advancing the digital and green transformation of public cultural buildings, supporting broader carbon neutrality and sustainable development objectives. Full article

The Dazhuzhuang, Biting, and Likou Sites are located along the Hui River basin in Yongcheng, eastern Henan. These three sites are situated close to each other and all yielded Longshan Culture period (2300–1800 BCE) remains, including large quantities of pottery with similar stylistic characteristics. However, archaeological surveys did not discover kiln sites at any of the three locations. To investigate the sources of Longshan period pottery in this region, its firing technology, and whether pottery circulated between the sites, this study employed a combination of X-ray fluorescence spectroscopy (XRF), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) to conduct a comprehensive scientific analysis of pottery unearthed from Longshan Culture contexts at the Dazhuzhuang, Likou, and Biting Sites in the Huai River basin, Yongcheng, Henan Province. The results reveal significant differences among the sites in terms of raw material selection, chemical composition, and technological characteristics. Pottery from the Dazhuzhuang Site exhibits with diverse clay sources. The Likou Site is characterized by highly homogeneous compositions derived from relatively high-alumina, low-iron clays, indicating standardized production practices. In contrast, the Biting Site shows greater variability in raw materials and functional differentiation. Thermal and microstructural analyses indicate that the dense glassy phase of black pottery was achieved through reducing firing conditions. In contrast, gray pottery was manufactured with calcareous additives to produce a porous structure. Full article

This is the second of the Dyes in History and Archaeology series of conference papers to be published as a Special Issue of Heritage; as before, its focus is on the use of dyes extracted from naturally occurring sources or early synthetic dyes [...] Full article

This article focuses on the model of a sailing ship from the collection of the Maritime Museum in Orebić, Croatia, whose sails conceal material, visual, and symbolic enigmas that have raised a number of new research questions. Particular attention was paid to the analysis of the sail substrate material, which was previously incorrectly catalogued as leather, while research has revealed that it is, in fact, impregnated canvas. Prolonged exposure to inadequate storage conditions led to material deterioration and visible changes that severely compromised the visual integrity of the model. A synthesis of laboratory analyses, conservation, and restoration studies, as well as historical and archival research in an interdisciplinary framework, made it possible to identify materials and manufacturing techniques in detail. The painted decorations on the sails and flags become clearly legible after cleaning, providing new information about the ship’s name and royal affiliation and opening up new avenues for investigating the symbolism behind the motifs of this model. Full article