Search Results (286)

Background/Objectives: Sepsis is a life-threatening condition characterized by an uncontrolled immune response due to systemic infections. It is responsible for millions of deaths worldwide. Although inflammasomes play an important role in host defense, they have a detrimental role in sepsis induced by LPS or Gram-negative bacteria. We aimed to revise the molecular mechanisms of inflammasome activation in sepsis by LPS and Gram-negative bacteria other than cytokine release as treatments blocking TNF-α and IL-1 cytokines have been ineffective even though cytokine storm is associated with lethality. Results: Studies with knockout mice deficient in inflammasome-derived cytokines have shown contrasting results on the role of these proinflammatory cytokines in the lethality of LPS- and Gram-negative-induced sepsis. However, DAMPs released after non-canonical inflammasome activation such as extracellular DNA, histones, HMGB1, and tissue factor result in disseminated-intravascular coagulation (DIC) and mortality in mice. Blocking these products in preclinical studies with animal models showed improved clinical scores and survival after LPS-induced sepsis or polymicrobial sepsis induced by Cecal Ligation and Puncture. Conclusions: Even though immunomodulatory drugs have shown inconclusive results as therapies for sepsis, blocking DAMPs associated with DIC may be considered for clinical trials in the future, especially in patients presenting biomarkers of coagulopathies. Full article

By using the Ekeland variational principle and Nehari manifold, we study the following fractional p-Laplacian Kirchhoff equations: $M\left({\left[u\right]}_{s,p}^p+{\int }_{{\mathbb{R}}^N}V\left(x\right){\left|u\right|}^{p}dx\right)[{(-\Delta )}_p^{s}u+$${V\left(x\right)\left|u\right|}^{p-2}u]={\lambda \left|u\right|}^{q-2}u\ln \left|u\right|,x\in {\mathbb{R}}^N,\left(\mathrm{P}\right)$. In these equations, $\lambda \in \mathbb{R}\setminus \left\{0\right\},$$p\in (1,+\infty )$, $s\in (0,1),sp<N,$$p_s^{*}={\displaystyle \frac{Np}{N-sp}}$, $M\left(\tau \right)=a+b{\tau }^{\theta -1}$, $a,b\in {\mathbb{R}}^{+},$$1<\theta <{\displaystyle \frac{p_s^{*}}{p}}$, $V\left(x\right)\in C({\mathbb{R}}^N,\mathbb{R})$ is a potential function and ${(-\Delta )}_p^s$ is the fractional p-Laplacian operator. The existence of solutions is deeply influenced by the positive and negative signs of $\lambda$. More precisely, (i) Equation (P) has one ground state solution for $\lambda >0$ and $p\theta <q<p_s^{*}$, with a positive corresponding energy value; and (ii) Equation (P) has at least two nontrivial solutions for $\lambda <0$ and $p<q<p_s^{*}$, with positive and negative corresponding energy values, respectively. Full article

Background/Objectives: Peripheral neuropathy (PN) is a common complication of diabetes mellitus (DM) with prevalence estimates showing considerable variation across studies. This study aimed to assess the prevalence and risk factors of PN in adult Greek subjects with type 2 diabetes mellitus (T2DM). Methods: Τhe PRENEDIG (PREvalence of peripheral NEuropathy in type 2 DIabetes in Greece) study was a nationwide, cross-sectional multicenter study based on data collected from hospital-based diabetes clinics and primary care practices from January 2024 to June 2024 in Greece. Diabetic peripheral neuropathy (DPN) prevalence and severity were evaluated using the Neuropathy Symptom Score (NSS) and the Neuropathy Disability Score (NDS). Additional sensory assessment tools were considered to support clinical evaluation. Multivariate regression analysis examined the association between DPN and potential risk factors. Results: Among the study population (n = 1807), the overall DPN prevalence was 18.87% and increased with longer diabetes duration. DPN prevalence among participants with over 10 years of T2DM reached 26.49%. Logistic regression analysis identified several independent predictors of DPN including diabetes duration > 10 years (p < 0.001), arterial hypertension in participants with diabetes duration < 10 years (OR = 2.69, CI: 1.68–4.30, p < 0.001), HbA1c levels (OR = 1.20, CI: 1.10–1.31, p < 0.001), and age (OR = 1.02, CI: 1.00–1.03, p = 0.024). An interaction-related association was observed, with arterial hypertension not increasing the risk of DPN any further in participants with disease duration > 10 years (OR: 3.73 vs. 3.80 with or without arterial hypertension, respectively). Sensory assessment tools further validated DPN diagnosis. Conclusions: In Greece, DPN is a common complication, affecting nearly one in five T2DM patients. The results of the study reinforce the importance of routine screening, particularly among older patients and those with longer diabetes duration to facilitate early detection and timely management of DPN and its associated complications. Full article

Video surveillance systems are a key tool for the identification of anomalous events, but they still rely heavily on human analysis, which limits their efficiency. Current video anomaly detection models aim to automatically detect such events. However, most of them provide only a binary classification (normal or anomalous) and do not identify the specific type of anomaly. Although recent proposals address anomaly classification, they typically require full video analysis, making them unsuitable for online applications. In this work, we propose a modular framework for the joint detection and classification of anomalies, designed to operate on individual clips within continuous video streams. The architecture integrates interchangeable modules (feature extractor, detector, and classifier) and is adaptable to both offline and online scenarios. Specifically, we introduce a multi-category classifier that processes only anomalous clips, enabling efficient clip-level classification. Experiments conducted on the UCF-Crime dataset validate the effectiveness of the framework, achieving 74.77% clip-level accuracy and 58.96% video-level accuracy, surpassing prior approaches and confirming its applicability in real-world surveillance environments. Full article

Inflammatory pain is a worldwide health problem, affecting primarily women. Nonetheless, most research conducted in this field has involved male subjects, and only 10% of the results have been obtained using females. Gold Lotion (GL) is a formulated product made from the peels of six citrus fruits that have been proven, via experimental data, to possess several biological properties, such as anti-inflammatory, anticancer, and antibacterial activities. This research aims to investigate the response to GL administration in two models of peripheral inflammation to assess potential sex differences. Carrageenan was used to induce mechanical allodynia and maximal edema within hours, serving as a model of acute inflammation, while Complete Freund’s Adjuvant (CFA) was used to obtain similar results within days, resulting in a model of chronic inflammation. Acute GL administration reduced mechanical allodynia in both models and both sexes, with no anti-inflammatory effects observed. In conclusion, this study demonstrates the potential of GL to alleviate inflammatory pain in both males and females, thereby broadening its therapeutic applications. Full article

LGBTQ+ individuals face substance use disparities linked to minority stress. While community belongingness may buffer stress, its role is complex. This study examined divergent associations between belongingness within the LGBTQ+ community and lifetime illicit drug use versus past-year alcohol frequency among LGBTQ+ adults in Kentucky (n = 2953), a region with notably high rates of substance use. Methods: Cross-sectional online survey data were analyzed. We measured LGBTQ+ belongingness, lifetime use of cocaine/crack/heroin/methamphetamine, and past-year alcohol frequency. Logistic and linear regressions controlled for age, education, gender identity, and income. Results: Greater belongingness predicted significantly higher odds of lifetime illicit drug use (OR = 1.24) but lower past-year alcohol consumption frequency (B = −0.094). Transgender and gender expansive identity predicted significantly higher illicit drug use odds and higher alcohol frequency. Conclusions: In this Kentucky sample, LGBTQ+ belongingness showed divergent substance use associations: it was protective against frequent alcohol use but, unexpectedly, was associated with higher odds of lifetime illicit drug use. Findings highlight belongingness’s complex, context-dependent nature and the need for nuanced research and interventions considering substance type and specific vulnerabilities, particularly for TGE individuals. Full article

Background: Bovine babesiosis, caused by the tick-borne apicomplexan parasite Babesia spp., is an economically significant disease that threatens the cattle industry worldwide. Babesia bovis is the most pathogenic species, leading to high morbidity and mortality in infected animals. One promising approach to vaccination against bovine babesiosis involves the use of multiple protective antigens, offering advantages over traditional live-attenuated vaccines. Tools such as immunobioinformatics and reverse vaccinology have facilitated the identification of novel antigens. Enolase, a “moonlighting” enzyme of the glycolytic pathway with demonstrated vaccine potential in other pathogens, has not yet been studied in B. bovis. Methods: In this study, the enolase gene from two B. bovis isolates was successfully identified and sequenced. The gene, consisting of 1366 base pairs, encodes a predicted protein of 438 amino acids. Its expression in intraerythrocytic parasites was confirmed by RT-PCR. Two peptides containing predicted B-cell epitopes were synthesized and used to immunize rabbits. Hyperimmune sera were then analyzed by ELISA, confocal microscopy, Western blot, and an in vitro neutralization assay. Results: The hyperimmune sera showed high antibody titers, reaching up to 1:256,000. Specific antibodies recognized intraerythrocytic merozoites by confocal microscopy and bound to a ~47 kDa protein in erythrocytic cultures of B. bovis as detected by Western blot. In the neutralization assay, antibodies raised against peptide 1 had no observable effect, whereas those targeting peptide 2 significantly reduced parasitemia by 71.99%. Conclusions: These results suggest that B. bovis enolase contains B-cell epitopes capable of inducing neutralizing antibodies and may play a role in parasite–host interactions. Enolase is therefore a promising candidate for further exploration as a vaccine antigen. Nonetheless, additional experimental studies are needed to fully elucidate its biological function and validate its vaccine potential. Full article

Antimicrobial food packaging with natural antimicrobials and biodegradable polymers presents an innovative solution to mitigate microbial contamination, prolong freshness, reduce food waste, and alleviate environmental burden. This study developed antimicrobial hemicellulose-based films by incorporating carvacrol (1% and 2%) as a natural antimicrobial agent through micro-emulsification produced by high-pressure homogenization (M-films). For comparison, films with the same formula were constructed using coarse emulsions (C-films) without high-pressure homogenization. These films were investigated for their antimicrobial efficacy, mechanical and barrier properties, and physicochemical attributes to explore their potential as sustainable antimicrobial packaging solutions. The M-films demonstrated superior antimicrobial activity, achieving reductions exceeding 4 Log CFU/mL against Listeria monocytogenes, Escherichia coli, and Salmonella enterica, compared to the C-films. High-pressure homogenization significantly reduced the emulsion’s particle size, from 11.59 to 2.55 μm, and considerably enhanced the M-film’s uniformity, hydrophobicity, and structural quality. Most importantly, the M-films exhibited lower oxygen transmission (35.14 cc/m²/day) and water vapor transmission rates (52.12 g/m²/day) than the C-films at 45.1 and 65.5 cc/m²/day, respectively, indicating superior protection against gas and moisture diffusion. Markedly improved mechanical properties, including foldability, toughness, and bubble-free surfaces, were also observed, making the M-films suitable for practical applications. This study highlights the potential of high-pressure homogenization as a method for enhancing the functional properties of hemicellulose-based films (i.e., M-films). The fabricated films offer a viable alternative to conventional plastic packaging, paving the way for safer and greener solutions tailored to modern industry needs. Full article

Accelerometry-based physical activity monitors (PAMs) are a useful tool to collect objective measurements of physical activity and movement. Recently, there has been an increased utilization of PAMs in companion animal chronic pain research. However, a general lack of understanding of PAMs contributes to challenges and misconceptions around the interpretation and utility of these data. Commercially available devices differ in how they acquire, process, report, and, in some cases, interpret data. Furthermore, various factors relating to the subject, such as age, body condition, and species, clearly influence PAM data, and on top of this, understanding the biological meaning of PAM data is in its relative infancy. This review examines the principles of PAM technology and the technical and biological considerations when applying PAMs to companion animal chronic pain research, in particular osteoarthritis pain research. It also provides an overview of applications of these devices in veterinary chronic pain research thus far, and the potential of these devices in future studies. Full article

Fish year-class strength (YCS) has been estimated via longitudinal analysis of catch-at-age data and via catch-curve regression, but no study has compared the two approaches. The objective of this study was to compare YCS estimates between the two approaches with application to the lake trout (Salvelinus namaycush) population in the main basin of Lake Huron, one of the Laurentian Great Lakes of North America. YCSs were reconstructed for both hatchery-stocked and wild lake trout. Akaike information criterion (AIC) and Bayesian information criterion (BIC) were used to compare 14 linear mixed-effects models for longitudinal analysis of catch-at-age data, and three linear mixed-effects models for catch-curve regression. From the best models based on AIC or BIC comparisons, YCS estimates with year-class as a fixed effect were consistent with those estimated with year-class as a random effect. Estimated YCS patterns and trends were the same or similar between the longitudinal analysis and the catch-curve regression, indicating that both approaches provide robust estimates of YCS. Potential bias in using the approach of catch-curve regression could be caused by abrupt changes in adult mortality. It is also critical to recognize multiple recruitment origins for using the approach of longitudinal analysis of catch-at-age data. Full article

The efficiency of in vitro maturation (IVM) in Cavia porcellus remains suboptimal compared to other species. This study aimed to characterize the morphological and functional quality of oocytes based on the stage of the estrous cycle and the classification of the cumulus–oocyte complexes (COCs) from which they were derived. A total of 744 oocytes were recovered postmortem from females in the diestrus and periovulatory phases. Oocytes were evaluated for metabolic activity, lipid distribution, apoptosis, nuclear maturation, and diameter. Oocytes collected during diestrus exhibited larger diameters and more homogeneous lipid distribution, particularly in oocytes from Type A COCs. In contrast, a higher proportion of BCB+ oocytes and reduced rates of early apoptosis were observed during the periovulatory stage, suggesting enhanced metabolic competence. Nuclear maturation rates varied with both cycle stage and COC classification, with oocytes from Type A COCs showing higher maturation rates in diestrus. These findings indicate that both intrinsic (e.g., morphological quality) and extrinsic (e.g., estrous cycle stage) factors modulated oocyte competence. Selecting oocytes based on integrated morphological and physiological criteria may improve the efficiency of IVM protocols in this species. Full article

Recently, the development of nanoparticle pigments has attracted interest in chemical preparation due to their potential functional properties, such as phosphate-based pigments. The present research focuses on the feasibility of synthesising the BaCr₂(P₂O₇)₂ pigment under hydrothermal conditions. The effect of the microstructural features of ceramic pigments (the crystalline structure, morphology, and particle size) on their optical properties (colour and reflectance) was also studied. The BaCr₂(P₂O₇)₂ compound was prepared in different fluid media, including water and NaOH solutions (0.5–1.0 M), at several reaction temperatures (170–240 °C) and intervals (6–48 h). The single-phase BaCr₂(P₂O₇)₂ did not crystallise without by-products (BaCr₂O₁₀, BaCr₂(PO₇)₂) in water and the alkaline solutions, even at 240 °C for 48 h; in these fluids, the ionic Cr³⁺ species oxidised to Cr⁶⁺. In contrast, the BaCr₂(P₂O₇)₂ single-phase crystallisation was favoured by adding urea as a reductant agent (25.0–300.0 mmol). Monodispersed BaCr₂(P₂O₇)₂ fine particles with a mean size of 44.0 nm were synthesised at a low temperature of 170 °C for 6 h with 0.5 M NaOH solution in the presence of 50.0 mmol urea. The phosphate pigment particle grew to approximately 62.0 nm by increasing the treatment temperature to 240 °C. A secondary dissolution–recrystallisation achieved after 24 h triggered a change in the particle morphology coupled with the incrementation of the concentration of NaOH in the solution. The pyrophosphate BaCr₂(P₂O₇)₂ pigments prepared in this study belong to the green colour spectral space according to the CIELab coordinates measurement, and exhibit 67.5% high near-infrared (NIR) solar reflectance. Full article

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline, memory loss and limited therapeutic options. Metal-based drugs have emerged as promising alternatives in the search for effective treatments, and vanadium coordination complexes have shown significant potential due to their neuroprotective and anti-aggregant properties. This review explores the advances in the development of vanadium-based metallodrugs for AD, focusing on their ability to modulate amyloid-beta (Aβ) aggregation, oxidative stress, and neuroinflammation. Recent in vitro and in vivo studies highlight the efficacy of oxovanadium (IV) and peroxovanadium (V) complexes in inhibiting Aβ fibril formation and reducing neuronal toxicity. Additionally, the interaction of vanadium complexes with key biological targets, such as peroxisome proliferator-activated receptor gamma (PPARγ) and protein-tyrosine phosphatase 1B (PTP1B), suggests a multifaceted therapeutic approach. While these findings underscore the potential of vanadium compounds as innovative treatments for AD, further research is needed to optimize their bioavailability, selectivity, and safety for clinical applications. Full article

Transglutaminase 2 (TG2) is a multifunctional protein and plays a role in cancer progression. We previously identified TG2 as an early-recurrence biomarker in hepatocellular carcinoma (HCC). TG2-knockdown (shTG2) and control (shCtl) HCC cell lines were used for comparative analyses to clarify the molecular mechanisms underlying the contribution of this protein to HCC malignancy. The proliferation of shTG2 cells was slightly but significantly decreased compared with that of shCtl cells. Differential gene expression profiling based on GeneChip arrays revealed the enrichment of the PI3K-Akt signaling pathway and showed that the expression of Dickkopf-1 and -3 (DKK1 and DKK3, respectively), inhibitors and modulators of the Wnt/β-catenin signaling pathway, was increased in shTG2 cells. The expression of epithelial–mesenchymal transition (EMT)-related genes was similar in both shCtl and shTG2 cells before and after TGF-β1 treatment, even though TGF-β1 markedly upregulated TG2. Thus, TG2 may contribute to cancer malignancy via the stimulation of cell proliferation signaling, such as PI3K-Akt and Wnt/β-catenin signaling, but not EMT. This effect might be further enhanced by humoral factors such as TGF-β1 from the tumor microenvironment. Full article

Hand gesture recognition (HGR) heavily relies on high-quality annotated datasets. However, annotating hand landmarks in video sequences is a time-intensive challenge. In this work, we introduce IPN HandS, an enhanced version of our IPN Hand dataset, which now includes approximately 700,000 hand skeleton annotations and corrected gesture boundaries. To generate these annotations efficiently, we propose a novel annotation tool that combines automatic detection, inter-frame interpolation, copy–paste capabilities, and manual refinement. This tool significantly reduces annotation time from 70 min to just 27 min per video, allowing for the scalable and precise annotation of large datasets. We validate the advantages of the IPN HandS dataset by training a lightweight LSTM-based model using these annotations and comparing its performance against models trained with annotations from the widely used MediaPipe hand pose estimators. Our model achieves an accuracy that is 12% higher than the MediaPipe Hands model and 8% higher than the MediaPipe Holistic model. These results underscore the importance of annotation quality in training generalization and overall recognition performance. Both the IPN HandS dataset and the annotation tool will be released to support reproducible research and future work in HGR and related fields. Full article