Search Results (1,544)

Background: Cancer therapeutics development has been a challenge in medical and scientific areas due to their toxicity, limited biocompatibility, and unfortunate side effects. However, despite advances in early detection and the study of novel treatments, the mortality rate for breast cancer remains high, making it a significant global health concern. Objectives: In this study, poly(methyl methacrylate) (PMMA) nanoparticles functionalized with acrylic acid (AA), fumaramide (FA), and curcumin (CUR) as chelating and inhibitor agents were synthesized by emulsion polymerization techniques. Methods and Results: Comprehensive physiochemical characterization studies based on gravimetry, dynamic light scattering (DLS), electrophoresis, Fourier transform infrared (FT-IR), ultraviolet–visible (UV–Vis) and photoluminescence (PL) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM) revealed a pH dependence of nanoparticles that exhibit structural changes upon interaction with calcium (Ca²⁺) and magnesium (Mg²⁺) ions. Calorimetric thermodynamic properties measured by isothermal titration calorimetry (ITC) confirmed chelating coordination and positive cooperativity between the nanoparticles and metal ions. In vitro studies showed the low cytotoxicity of nanoparticles by fibroblast proliferation, and their chelation process was observed by fluorescence microscopy, with the loss of interaction between cells. Conclusions: These results suggest that the functionalized nanoparticles have potential in drug delivery systems (DDS) for targeted breast cancer therapies, providing a promising polymer material for more efficient and less toxic treatments. Full article

Protected areas are essential for conserving biodiversity and sustaining ecosystems, yet their effective management requires a clear understanding of soil and water quality, which underpin ecological processes. This study evaluated 15 soil and seven water samples to assess their physico-chemical properties, focusing on heavy metal concentrations. Results showed that soils were generally neutral to alkaline, with Hashwan-2 exhibiting the highest concentrations of calcium (26.5 meq/L), magnesium (11.2 meq/L), carbonates (0.32 meq/L), bicarbonates (3.66 meq/L), and chloride (35.43 meq/L). Heavy metal analysis indicated elevated nickel (51.628 mg/kg) and chromium (76.29 mg/kg) at Albuyitlar-2, and chromium (68.015 mg/kg) at Shabak-Mateam-1 1 1, exceeding US-EPA permissible limits of 45 mg/kg for nickel and 64 mg/kg for chromium. Water samples revealed high levels of aluminum (12.681 mg/L), manganese (0.146 mg/L), and iron (7.055 mg/L), also exceeding the US-EPA thresholds of 0.2, 0.1, and 0.5 mg/L, respectively. In contrast, more toxic metals such as arsenic, cadmium, lead, and mercury remained within safe limits. These findings highlight localized concerns regarding heavy metal contamination that warrant continued monitoring to ensure ecosystem health. Full article

Continuous cropping can often deteriorate soil quality and reduce crop yield. Soil properties and microbial communities usually play a vital role in maintaining rhizosphere micro-ecosystem sustainability, which is yet to be addressed in continuous peony monoculture systems. Herein, variations in soil physiochemical properties were extensively investigated following 1, 4, and 10 years of continuous tree peony cropping, as well as microbial community diversity, composition, and predicted functions. The soil pH and contents of available Mg, Mn, Zn, and B significantly declined after 10 years of continuous monoculture, while the contents of soil organic carbon, nitrate, and available P, K, Fe, and Cu notably increased by more than 100%, implying an imbalance of soil nutrients resulting from long-term continuous cropping. High-throughput sequencing results indicated that the microbial community structure and composition were remarkably altered after either 4 or 10 years of continuous cropping, interfering with diverse microbial metabolic pathways and phenotype functions. In addition, the relative abundances of some beneficial bacteria dramatically increased, especially for Acidobacteriota and Bacillus members. Microbial selections or adaptations in response to soil nutrient changes were expected to remediate negative impacts of continuous cropping on soil quality. Findings in this study provide insights into the establishment of proper management strategies for sustaining soil quality to resist potential obstacles after long-term continuous peony cropping. Full article

Background/Objectives: Radotinib is a second-generation tyrosine kinase inhibitor (TKI) that has been used for treatment of chronic myeloid leukemia (CML). This study was performed for the first time to characterize the pharmacokinetics of radotinib, identify the factors contributing to pharmacokinetic variabilities and explore alternative dosing regimens. Methods: A total of 640 plasma concentration–time datapoints obtained from 47 participants were evaluated using nonlinear mixed-effects modeling to estimate pharmacokinetic parameters and evaluate covariate effects. The study population comprised 23 healthy volunteers (HVs) who received a single, oral dose of 400 mg radotinib and 24 CML patients who repeatedly received 300 mg twice daily. Based on the final population pharmacokinetic model, alternative dosing regimens to the current every 12 h regimen were explored using Monte Carlo simulations. Results: A two-compartment model with first-order absorption through transit compartments and first-order elimination incorporating a circadian rhythm effect best described radotinib pharmacokinetics. Disease status significantly affected apparent clearance; it was slower by 39.2% in CML patients compared with HVs (23.0 L/h versus 37.9 L/h), resulting in a longer terminal half-life (28.8 h versus 17.5 h). Age was negatively associated with volume of distribution in the central compartment, with an estimated slope of −0.0129 L/year. A 400 mg once-daily regimen was predicted to provide comparable systemic exposures to those of other TKIs with similar physiochemical and pharmacological properties to radotinib, and a 36% lower exposure than that of the current 300 mg twice-daily regimen. Conclusions: The model developed in this study adequately describes the population pharmacokinetics of radotinib and provides a basis for optimal, individualized radotinib therapy for patients with CML. Full article

In this present study, the efficiency of ultrasound-assisted extraction (UAE) in increasing the yields of extraction of starch and polyphenols from Canna indica L. (Canna) rhizomes were analyzed, along with its influence on the physiochemical properties of the extracted compounds. Extraction parameters (temperature, time, and solid-to-liquid ratio) were optimized through Box–Behnken response surface design (BBD). The physiochemical and functional properties of starch and polyphenols were investigated through scanning electron microscopy (SEM), the swelling and solubility index, oil and water absorption index, total polyphenol yield, and antioxidant activity assays (DPPH and ORAC). The starch yield obtained from Canna at the optimum extraction conditions (temperature 40 °C, time 10 min, and solid-to-liquid ratio 1:30 g/mL) was 19.81%. The obtained starch yield was found to be significantly higher than the yield attained through the conventional extraction method without adverse changes in the physicochemical and functional properties. The total polyphenol extraction yield from the Canna rhizome, through UAE, was significantly higher (1061.72 mg GAE/100 g) than that of the conventional method. The antioxidant activity of bioactive compounds was proportional to the attained polyphenol yield. Our results suggest that UAE optimized conditions efficiently and improved Canna starch and polyphenol extraction yields while preserving their functional properties. Full article

In this paper, different physiochemical and biological indicators were tested to determine and compare the water quality of the Zaalklapspruit ecologically engineered wetland before and after a veldfire. Five sampling sites and a reference site 2.2 km upstream of an acid mine drainage (AMD)-decanting coal mine were selected and sampled before and after the veldfire. The “black box” method was also employed to determine the percentage change in the selected in- and outflow variables before and after the veldfire. After the veldfire, Al was reduced by 97.43%. The same trend was observed for Fe, which decreased by 99.65% at the outflow, and Mn and sulphate levels decreased by 98.41% and 68.16%. Possible pathways of the reduction in acid mine drainage impacts on the wetland were identified after the veldfire, including the increase in waterflows during the wet season causing a dilution factor, and phycoremediation by macroalgae drifting mats that accumulate metals and ash slurry from the burned-out macrophyte plant material that may have increased the wetland’s alkalinity. A comprehensive framework for the digital twinning and monitoring of the effects of natural disasters on wetlands is also presented. Full article

The demand for sustainable protein has increased interest in edible insects, and fermentation can improve the sensory and nutritional profiles of novel foods. This study aimed to develop a fermented cricket paste using a method analogous to traditional shrimp paste and evaluate the physiochemical, nutritional, microbiological, and sensory properties. Both pastes were produced via a biphasic fermentation protocol and subsequently analyzed for their physicochemical, nutritional, microbiological, and sensory properties including consumer acceptance testing in a chili paste. The cricket paste showed a comparable protein content, but higher in carbohydrates and lower in fat. It also showed enhanced stability and safety, with a significantly lower level of histamine (2.37 ppm) compared with shrimp paste (50.51 ppm). While the microbial profiles were broadly similar, the cricket paste had a lower lactic acid bacteria community. Sensory analysis revealed distinct profiles, with the cricket paste characterized by a dark color, coarse texture, nutty/earthy aroma, bitter, and less umami. Despite these differences, consumer liking for chili pastes made with either product was statistically identical. Cricket paste is therefore a safe, stable, and nutritionally advantageous alternative that is highly acceptable to consumers when used as a culinary ingredient. Full article

In this study, a slow-release fertilizer (SRF) was obtained by occluding NPK 10–10–10 into two matrices and compared with the uncoated mineral fertilizer (F). The first matrix, FOMI, used biosolids/paper sludge at 3:1 (w/w); the second, FOMII, used biosolids/clay at 1:1 (w/w). Materials and pellets were physiochemically and microbiologically characterized. Release kinetics were evaluated in water and in soil columns packed with acid-washed sand; matrix-only controls and sand blanks confirmed negligible background N, P, and K. The uncoated mineral fertilizer (F) showed a rapid burst, whereas occlusion slowed release. FOMII reduced release relative to F, and FOMI produced the slowest, controlled profiles: kinetic fits yielded lower k values for FOMI than for FOMII and F. FOMI also exhibited higher water-retention capacity (WRC) and cation-exchange capacity (CEC), consistent with its greater organic-matter content. In soil, FOMI released less than 15% at 48 h and no more than 75% at 30 d, meeting European Committee for Standardization (CEN) SRF criteria; FOMII released faster than FOMI but slower than F, which exceeded 90% within the test period. Therefore, FOMI is a biodegradable, low-cost SRF that improves fertilizer-use efficiency while returning organic matter to agricultural soils; FOMII shows intermediate yet beneficial performance. Full article

Zinc (Zn) is an essential micronutrient for plant growth, serving as a co-factor in enzymatic processes and pigment biosynthesis. In horticultural crops such as lettuce, Zn fertilization is increasingly relevant for optimizing yield and nutritional quality. In this study, a greenhouse pot experiment was conducted using Lactuca sativa L. cv. Romana Verano (Ramiro Arnedo) to evaluate the effects of four Zn sources with contrasting physio-chemical properties—ZnSO₄, a synthetic chelate containing DTPA, EDTA, and HEDTA, a Zn–lignosulphonate complex, and ZnO nanoparticles—applied to soil at rates of 15, 30, 60, and 120 mg Zn·kg⁻¹. Morphometric traits, photosynthetic pigmentation, and photosystem performance were assessed to determine differences in plant response. Results showed that low to moderate Zn supply (15–60 mg Zn·kg⁻¹) maintained growth, leaf number, stem diameter, and biomass without significant changes compared to the control. In contrast, the highest dose (120 mg Zn·kg⁻¹), particularly in chelated forms, led to reductions in growth and yield exceeding 80%, reflecting supra-optimal effects. Although lignosulphonate and nanoparticles sources lowered soil Zn availability, they did not affect lettuce growth or yield, indicating their potential as safer agricultural alternatives to conventional Zn fertilizers. Photosynthetic efficiency, measured through chlorophyll fluorescence and electron transport activity, was positively modulated by adequate Zn levels but declined at excessive concentrations. These findings highlight that Zn efficiency strongly depends on its chemical form and applied dose, providing practical insights for optimizing Zn fertilization strategies in lettuce and other horticultural crops. Full article

Background: Measurement uncertainty (MU) is a crucial parameter for ensuring the reliability of analytical methods and the validity of results, as required by ISO 17025:2017. Its estimation is particularly critical for quality control laboratories, where compliance decisions are based on a rigorous interpretation of uncertainties. Methods: In this study, we evaluated the uncertainty associated with an HPLC-UV method for the determination of Metopimazine (MPZ) in a pharmaceutical form, applying two complementary approaches: The ISO-GUM (Guide to the Expression of Uncertainty in Measurement) top-down approach and the Monte Carlo Simulation (MCS). Results: The results of both approaches showed excellent agreement, thus validating the robustness of the evaluation. The analysis of uncertainty sources revealed that the accuracy of the sample volume (V_Sample) and the calibration standard (C_x) were the dominant contributors, representing 39.9% and 36.2% of the total uncertainty, respectively. Combined, these two factors accounted for 76.1% of the variability, underscoring their critical impact on the assay’s precision. The expanded uncertainty (k = 2, 95% confidence level) was determined to be (99.41 ± 0.69)%, reflecting the method’s reproducibility. Conclusions: These results highlight the importance of rigorously controlling calibration standard preparation, sample volume, and repeatability conditions to optimize the reliability of the assay. Full article

Background/Objectives: The search for new bioactive molecules increasingly extends beyond conventional medicinal plants, highlighting the importance of exploring alternative botanical sources. Parasitic plants represent a promising but underexploited reservoir of pharmacologically relevant compounds. Cuscuta australis (CA), a parasitic species with a history of traditional use, remains poorly characterized. This study aimed to investigate its phytochemical composition and evaluate its antioxidant, anti-inflammatory, and hepatoprotective properties. Methods: The phytochemical profile of CA extract was characterized by LC-MS. Antioxidant capacity was assessed using DPPH and ABTS assays. In vivo hepatoprotection was evaluated in male rats subjected to CCl₄-induced hepatotoxicity and treated orally with CA (30 or 60 mg/kg body weight). Biochemical, lipid, oxidative stress, and histological parameters were determined. Molecular docking was conducted to predict the binding of major identified compounds against selected protein targets. Results: CA significantly and dose-dependently improved biochemical and histological markers. At 60 mg/kg, ALT, AST, ALP, and bilirubin were reduced by 32%, 33%, 63%, and 51%, respectively. Lipid metabolism was improved by decreased TC, TG, and LDL-C with increased HDL-C. Antioxidant defense was enhanced through elevated CAT, SOD, and GPx activities, accompanied by reduced MDA levels. TNF-α and IL-6 decreased by 48% and 53%, respectively. Histopathology confirmed hepatoprotection and reduced fibrosis. Docking studies revealed strong binding affinities (−7.07 to −19.20 kcal/mol) for several metabolites, notably quercetin glucoside, diosmetin glucoside, caffeic acid glucoside, feruloylquinic acid, and isorhamnetin glucoside, against CYP450, IL-2, TNF-α, and IL-6. Conclusions: These findings demonstrate that C. australis is a promising source of bioactive compounds with hepatoprotective, antioxidant, antihyperlipidemic, and anti-inflammatory effects, supporting its potential as a natural therapeutic agent. Full article

Microplastics (<5 mm) and nanoplastics (~100 nm), which are invisible to the naked eye, originate primarily from fragmentation and breakdown larger plastic debris are increasingly pervasive in the environment. Once released, they can disperse widely in the environment, pollute them adversely and ultimately be taken up by living organisms, including humans, through multiple exposure pathways. Their distribution in aquatic systems is influenced by their physiochemical properties including density, hydrophobicity, and chemical stability, along with environmental conditions and biological activities. To better understand the dynamics of micro- and nanoplastics in surface water, this study conducted a comprehensive review of 194 published articles and scientific reports covering marine, freshwater, and wastewater systems. We assessed the abundance, spatial distribution and the factors that govern their behavior in aquatic systems and analyzed the sampling techniques, pretreatment process, and detection and removal techniques to understand the ongoing scenario of these pollutants in surface water and to identify the ecological risks and potential toxicological effects on living biota via direct and indirect exposure pathways. Full article

As the demand for chitin grows, new chitin sources with unique physicochemical properties are required. Abundant biofouling species, such as amphipods and hydroids, have chitinous skeletal systems that can be utilized for chitin production. However, little is known about these chitin sources. This study investigated the viability of amphipods (Jassa sp.) and hydroids (Coryne sp.) obtained from aquaculture biofouling assemblages as novel sources of chitin. Chitin was extracted from these sources and characterized in terms of its degree of acetylation (DA), crystallinity index (CrI), molecular weight (MW), thermal stability, and surface morphology. Physiochemical characteristics where then compared against commercially available shrimp chitin. Results show that a 32.75% chitin yield can be obtained from hydroids. The percentage DA for amphipod (AC) and hydroid (HC) chitin is 58.4–59.2% and 64.8–66.7%, respectively. AC is characterized as α-chitin with a low molecular weight (MW), while HC is medium-MW β-chitin. This finding is significant because it shows hydroids to be a new source of rare β-chitin. In addition, AC has higher thermal stability than HC. AC and HC greatly differ in terms of surface morphology. Therefore, the chitin biomaterials extracted from amphipods and hydroids have different but favorable properties that can be used for diverse applications. Full article

Morchella spp. is a type of valuable and rare edible fungi cultivated in soil. Optimization of the cultivation medium for Morchella spp. is key to obtaining high-efficiency production in an ecologically friendly manner. Recently, the sustainable resource utilization of agricultural waste has gathered attention. Specifically, reusing tomato substrate, mushroom residues, and coconut shells can lower the production costs and reduce environmental pollution, demonstrating remarkable ecological and economic benefits. To determine the soil microbial communities of Morchella spp. using different culture medias and influencing factors, this study analysed the relative abundance of bacterial and fungal communities in natural soil, soil with 5% tomato substrate, soil with 5% mushroom residues, and soil with 5% coconut shells using Illumina NovaSeq high-throughput sequencing. In addition, intergroup differences, soil physiochemical properties, and product quality were also determined. Results demonstrated that agricultural waste consisting of mushroom residues, waste tomato substrate, and coconut shells can improve the efficiency of Morchella spp. cultivation. When considering yield and quality, mushroom residue achieved the highest yield (soil nutrient enrichment), followed by tomato substrate (water holding + grass carbon nutrient). All three types of agricultural waste promoted early fruiting, significantly increased polysaccharide, crude protein, and potassium content, and lowered crude fat and fibre. In regard to soil improvement, the addition of different materials optimized the soil’s physical structure (reducing volume weight and increasing water holding capacity) and chemical properties (enrichment of nitrogen, phosphorus, and potassium, regulating nitrogen and medium trace elements). For microbial regulation, the added materials significantly increased the abundance of beneficial bacteria (e.g., Actinomycetota, Gemmatimonadota and Devosia) and strengthened nitrogen’s fixation/nitration/decomposition functions. In the mushroom residue group, the abundance of Bacillaceae was positively related to yield. Moreover, it inhibited pathogenic fungi like Mortierella and Trichoderma, and lowered fungal diversity to decrease ecological competition. In summary, mushroom residues have nutrient releasing and microbial regulation advantages, while tomato substrate and coconut shells are new high-efficiency resources. These increase yield through the “physiochemical–microorganism” collaborative path. Future applications may include regulating the function of microorganisms and optimizing waste preprocessing technologies to achieve sustainability. Full article

Heavy metal (HM) contamination of soil is a worldwide problem with adverse consequences for the environment and human health. Microorganisms, as the most active fraction in soil, play a pivotal role in assessing changes in soil quality and maintaining ecological equilibrium. Accordingly, screening efficient microorganisms for remediating contaminated soils has emerged as a key research focus. This study employed high-throughput sequencing and conducted in situ field surveys to investigate the impacts of long-term HM pollution with varying severity on soil physicochemical properties, as well as the community structure and diversity of bacteria and fungi. The results showed that the major soil physiochemical properties and the bacterial and fungal β diversity significantly changed with the increase in HM pollution levels. The relative abundances of Chloroflexi, Myxococcota and Nitrospirota among bacteria, along with Chytridiomycota and Talaromyces among fungi, increased significantly with rising HM pollution levels. In low-, medium- and highly contaminated soils, the dominant bacterial species were OTU10618 (Micrococcales), OTU6447 (Chthoniobacterales), and OTU7447 (Burkholderiales), while the dominant fungal species were OTU3669 (Glomerellales), OTU397 (Olpidiales), and OTU2568 (Mortierellales). Bacterial communities were mainly affected by soil-available phosphorus, available cadmium (Cd) and available Pb, while fungal communities were predominantly influenced by soil-available phosphorus, soil organic carbon and total Pb content. These findings demonstrate that soil microorganisms in chronically HM-contaminated soils exhibit adaptive shifts, and this study thereby provides critical implications for assessing the remediation potential of diverse microbial taxa in HM-polluted soils. Full article