Search Results (9,673)

Pepper (Capsicum spp.) is an economically valuable crop worldwide including in the United States due to its nutritional benefits in human health and widespread use as a spice or vegetable. Although numerous viruses have been reported infecting peppers in the USA, little is known about the diversity and distribution of pepper-infecting viruses in Oklahoma. To address this knowledge gap, we conducted a comprehensive pepper virome study to identify viruses infecting pepper and their incidence across six different counties in Oklahoma. A total of 310 plant samples including pepper and other potential hosts were collected during the 2021 and 2022 growing seasons. Samples were analyzed using high-throughput sequencing (HTS) and/or reverse transcription-polymerase chain reaction (RT-PCR) assays. Viral contigs identified via HTS were further validated through RT-PCR or PCR assays followed by Sanger sequencing. In total, 17 distinct viruses were detected, including 15 RNA and two DNA viruses, with several representing putatively novel findings. The most prevalent virus was beet curly top virus (BCTV), followed by tomato yellow leaf curl virus (TYLCV), potato yellow dwarf virus/constricta yellow dwarf virus (PYDV/CYDV), and pepper mild mottle virus (PMMoV). Virus incidence varied by season and location, with some surveys showing infection rates exceeding 80%. This study provides the first in-depth characterization of the pepper virome in Oklahoma and valuable insights into the prevalence and distribution of pepper-infecting viruses. These findings will support the development of informed, targeted strategies for virus detection and management in pepper production systems. Full article

This study suggests a newly developed model for estimating city-scale photovoltaic rooftop energy potential. This model aims to provide reasonable universal calculations regarding a city’s available space for mounting rooftop photovoltaic systems and their corresponding annual electricity production capacity. For the development of the model, a thorough literature review has been conducted, which compiles and presents mathematical expressions and performance coefficients. Necessary geographic and meteorological data have been obtained from European statistical repositories and the PVGIS tool, respectively. The main inputs refer to a city’s basic geographical data, population, total actual area, geographical coordinates, and, by extension, the optimum PV unit installation angle. This analysis presents a simple and accurate model applicable to European cities for assessing rooftop photovoltaic energy potential and suitable rooftop space for PV units. The findings can aid in advancing PV development in urban areas and contribute to creating environmentally neutral cities in the future. The methodology is verified with data retrieved from the Google Environmental Insights Explorer tool, which shows a deviation of 9.72%. According to the computational analysis for 40 European countries, the photovoltaic energy potential is between 12.31 GWh and 8200 GWh. These values correspond to a net available PV space between 0.03 km² and 31.86 km². The greatest photovoltaic coverage potential is equal to 117.4% for Patras, Greece, while the lowest is 7.27% for Oslo, Norway. Regarding the avoided greenhouse gas emissions, they are found to vary from 5.8 ktons of CO_2-equivalent for Valletta, Malta, and 8109.8 ktons for the city of London, United Kingdom. Finally, the final results of 86 additional cities located on the European continent are given. Full article

As sessile organisms, plants have developed strategies to cope with exposure to high radiation. The plant cuticle is located at the interface between the plant and the surrounding environment, thus acting as a first barrier that protects plants against environmental conditions, including solar radiation. The isolated cuticles displayed notable absorptance in the infrared spectral range which, according to Kirchhoff’s law of thermal radiation, equals the emission dissipation ability. Comparison among the different cuticles showed that a significant range of their reflectance, transmittance, and absorbance spectra match the spectral regions known as atmospheric windows, between 3–4 and 8–13 microns, located within the mid-infrared region (MIR). They allow energy to pass through into the outer space. These optical parameters varied between cuticles from different plant species and they were not a simple function of the cuticle’s thickness but the product of its specific composition in combination with its molecular arrangement. Full article

Livestock represent a significant source of methane (CH₄) emissions, particularly in pastoral regions. However, in Xinjiang—a pivotal pastoral region of China—the spatiotemporal patterns of livestock CH₄ emissions remain poorly characterized, constraining regional mitigation actions. Here, a detailed CH₄ emissions inventory for livestock in Xinjiang spanning the period 2000–2020 is compiled. Eight livestock categories were covered, gridded livestock maps were developed, and the dynamic emission factors were built by using the IPCC 2019 Tier 2 approaches. Results indicate that the CH₄ emissions increased from ~0.7 Tg in 2000 to ~0.9 Tg in 2020, a 28.5% increase over the past twenty years. Beef cattle contributed the most to the emission increase (59.6% of total increase), followed by dairy cattle (35.7%), sheep (13.9%), and pigs (4.3%). High-emission hotspots were consistently located in the Ili River Valley, Bortala, and the northwestern margins of the Tarim Basin. Temporal trend analysis revealed increasing emission intensities in these regions, reflecting the influence of policy shifts, rangeland dynamics, and evolving livestock production systems. The high-resolution map of CH₄ emissions from livestock and their temporal trends provides key insights into CH₄ mitigation, with enteric fermentation showing greater potential for emission reduction. This study offers the first long-term, high-resolution CH₄ emission inventory for Xinjiang, providing essential spatial insights to inform targeted mitigation strategies and enhance sustainable livestock management in arid and semi-arid ecosystems. Full article

Oenothera biennis L. is a biennial species native to North America and introduced to Europe in the 17th–18th centuries, used in traditional medicine or as a dietary supplement in various products, as well as in cosmetics and the pharmaceutical industry. In recent decades, oil extracted from seeds has been increasingly used for the treatment of various conditions. In this article, we highlight the polyphenolic content of 2 types of extracts from O. biennis species, collected from the wild flora of Romania, from two regions with different altitudes and pedoclimatic conditions (plains and mountains), namely: OHM—hydroalcoholic extract, Macea sample; OHVD—hydroalcoholic extract, Vatra Dornei; OAM—aqueous extract, Macea sample; OAVD—aqueous extract, Vatra Dornei sample. The LC/MS analysis of the whole plant extracts revealed 5 to 14 polyphenols, depending on the sample location and type of extract, out of which 7 flavonoids are newly reported for this species. Climatic parameters were mapped using QGIS. Higher qualitative and quantitative values of polyphenols were observed in the hydroalcoholic extract obtained from individuals collected from the plain area. Full article

The Molise region in Central-Southern Italy is a major contributor to national truffle production, particularly of the highly prized Tuber magnatum Picco, accounting for approximately 40% of the country’s total output and hosting the highest density of truffle harvesters. Despite this, research on the Italian white truffle populations from this area remains limited. Therefore, the primary objective of the present study was to address this knowledge gap by characterizing four T. magnatum Picco populations collected from the municipalities of Agnone, Carovilli, Castel del Giudice, and Pietrabbondante, located in “Alto Molise”, through morphological, genetic, and microbiological investigations. The statistical analyses revealed significant differences in peridium thickness and ascocarp-associated microbiota even though pairwise comparisons did not identify statistically significant differences between specific population pairs. No significant variation was observed in ascocarp weight and maturation degree. Furthermore, the presence of a unique haplotype at the single-locus marker SCAR A21-inf was confirmed in a subset of the analyzed fruiting bodies. Collectively, these findings expand current biological knowledge of the Molise white truffle and provide a foundation for future research aimed at identifying specific provenance markers to discriminate truffle populations at both regional and local scales. Full article

This study evaluates the feasibility of employing rainwater as an alternative feedstock for hydrogen production via electrolysis. While conventional systems typically rely on high-purity water—such as deionized or distilled variants—these can be cost-prohibitive and environmentally intensive. Rainwater, being naturally available and minimally treated, presents a potential sustainable alternative. In this work, a series of comparative experiments was conducted using a proton exchange membrane electrolyzer system operating with both deionized water and rainwater collected from different Austrian locations. The chemical composition of rainwater samples was assessed through inductively coupled plasma, ion chromatography and visual rapid tests to identify impurities and ionic profiles. The electrolyzer’s performance was evaluated under equivalent operating conditions. Results indicate that rainwater, in some cases, yielded comparable or marginally superior efficiency compared to deionized water, attributed to its inherent ionic content. The study also examines the operational risks linked to trace contaminants and explores possible strategies for their mitigation. Full article

Heike 88, a new soybean variety developed through strategic hybridization of Heijiao 08-1611 × Heihe 43 followed by pedigree selection, was evaluated across seven locations in Heilongjiang Province from 2019 to 2022. The variety demonstrated stable performance with a 10.3% average yield advantage over regional check varieties and mean yields of 3188 kg ha⁻¹. Principal component analysis revealed that genetic variation accounted for 43.4% and 32.6% of performance variance in the first two components, indicating successful transgressive segregation where the pure line exceeded both parental lines through complementary gene action. Performance relative to parental averages ranged from −20% to +40% across the temperature gradient, demonstrating strong genotype-environment interaction effects. Machine learning analysis identified year effect (13% importance), accumulated temperature (7.6% importance), and oil content (4% importance) as primary yield drivers. Complete resistance to soybean mosaic virous (SMV) and cyst nematode attack was observed across all locations, with excellent gray leaf spot resistance (grades 0–1) maintained under natural pathogen pressure. Seed quality parameters remained stable across environments, with protein content ranging from 41.69% to 42.25% and oil content from 19.74% to 20.13%, indicating minimal environmental effects on compositional traits. Yield stability improved progressively over the evaluation period, with the coefficient of variation decreasing from 18.7% in 2019 to 6.7% in 2022, while absolute yields increased from 2550 to 3200 kg ha⁻¹. These results demonstrate successful exploitation of transgressive segregation for regional adaptation through strategic parent selection and pedigree breeding, supporting commercial deployment in northern China’s challenging production environments while providing methodological guidance for future breeding programs targeting environmental specificity. Full article

Xylella fastidiosa was first detected in Portugal in 2019 in Lavandula dentata. In response, the national plant health authorities promptly established a Demarcated Zone in the affected area and implemented a series of eradication and control measures, including the systematic removal and destruction of infected and host plants. This study analyzes the economic and operational impacts of these eradication efforts in the northern region of Portugal, with a focus on Demarcated Zones such as the Porto Metropolitan Area, Sabrosa, Alijó, Baião, Mirandela, Mirandela II, and Bougado between 2019 and June 2023. During this period, about 412,500 plants were uprooted. The majority were Pteridium aquilinum (bracken fern), with 360,324 individuals (87.3%), reflecting its wide distribution and the large area affected. Olea europaea (olive tree) was the second most common species removed, with 7024 plants (1.7%), highlighting its economic relevance. Other notable species included Quercus robur (3511; 0.85%), Pelargonium graveolens (3509; 0.85%), and Rosa spp. (1106; 0.27%). Overall, destruction costs were estimated at about EUR 1.04 million, with replanting costs of roughly EUR 6.81 million. In parallel, prospection activities—conducted to detect early signs of infection and monitor disease spread—generated expenses of roughly EUR 5.94 million. While prospecting represents a significant financial investment, the results show that it is considerably more cost-effective than large-scale eradication. Prospection enables early detection and containment, preventing the widespread destruction of vegetation and minimizing disruption to agricultural production, biodiversity, and local communities. Importantly, our findings reveal a sharp decline in confirmed cases in the initial outbreak area—the Porto Demarcated Zone—from 124 cases in 2019 to just 5 in 2023, indicating the effectiveness of the eradication and monitoring measures implemented. However, the presence of 20 active Demarcated Zones across the country as of 2023 highlights the continued risk of spread and the need for sustained vigilance. The complexity of managing Xylella fastidiosa across ecologically and logistically diverse territories justifies the high costs associated with surveillance and targeted interventions. This study reinforces the strategic value of prospection as a proactive and sustainable tool for plant health management. Effective surveillance requires the integration of advanced methodologies aligned with the phenological stages of host plants and the biological cycle of vectors. Targeting high-risk locations, optimizing sample numbers, ensuring diagnostic accuracy, and maintaining continuous training for field teams are critical for improving efficiency and reducing costs. Ultimately, the findings underscore the need to refine and adapt monitoring and eradication strategies to contain the pathogen, safeguard agricultural systems, and prevent Xylella fastidiosa from becoming endemic in Portugal. Full article

Despite converting large, laterally unbounded, highly connected low-permeability lithologic gas reservoirs—without faults or fixed lithological boundaries—into underground gas storage, the evolution of transition zone pressures and the mechanisms of gas escape under multiple injection–production cycles remain poorly understood. This knowledge gap critically hinders the safe and efficient operation of such facilities. A core–transition zone injection–withdrawal model for the S4 underground gas storage was developed using the numerical well test module of Saphir software v4.20. The model quantifies transition zone pressure dynamics over ten injection–withdrawal cycles and elucidates how the interplay of formation permeability and operating conditions governs gas leakage. During multi-cycle injection–withdrawal operations, formation pressure in the transition zone steadily accumulates under the combined influence of core zone gas crossflow and local gas advection equilibrium within the non-utilizable region. Assessed by the transition zone boundary formation pressure, suppressing gas leakage depends primarily on total injection and withdrawal volume, followed by the injection schedule and, lastly, the location of the boundary injection well. To achieve cost-effective containment, we therefore recommend prioritizing a shorter injection duration, moderately reducing total injection and withdrawal volume, and increasing the distance between the boundary injection wells and the transition zone. Under the geological conditions of the S4 UGS, by sequentially adjusting the injection duration, reducing the total injected–withdrawal gas volume to 6000 × 10⁴ m³, and increasing the distance between boundary injection wells and the transition zone to 900 m, the transition zone boundary pressure rise over ten cycles was controlled to below 1 MPa, thereby effectively preventing gas leakage. Full article

Population growth drives increasing energy demands, agricultural production, and organic waste generation. The organic waste contributes to greenhouse gas emissions and increasing landfill burdens, highlighting the need for novel closed-loop technologies that integrate water, energy, and food resources. Within the context of the Water–energy–food Nexus (WEF), wastewater can be recycled for food production and food waste can be converted into clean energy, both contributing to environmental impact reduction and resource sustainability. A novel household-scale, closed-loop WEF system was designed, installed and operated to manage organic waste while retrieving water for irrigation, nutrients for plant growth, and biogas for energy generation. The system included a biodigester for energy production, a sand filter system to regulate nutrient levels in the effluent, and a hydroponic setup for growing food crops using the nutrient-rich effluent. These components are operated with a daily batch feeder coupled with automated sensors to monitor effluent flow from the biodigester, sand filter system, and the feeder to the hydroponic system. This novel system was operated continuously for two months using typical household waste composition. Controlled experimental tests were conducted weekly to measure the nutrient content of the effluent at four locations and to analyze the composition of biogas. Gas chromatography was used to analyze biogas composition, while test strips and In-Situ Aqua Troll Multi-Parameter Water Quality Sonde were employed for water quality measurements during the experimental study. Experimental results showed that the system consistently produced biogas with 76.7% (±5.2%) methane, while effluent analysis confirmed its potential as a nutrient source with average concentrations of phosphate (20 mg/L), nitrate (26 mg/L), and nitrite (5 mg/L). These nutrient values indicate suitability for hydroponic crop growth and reduced reliance on synthetic fertilizers. This novel system represents a significant step toward integrating waste management, energy production, and food cultivation at the source, in this case, the household. Full article

Under the background of global climate change, lichens as a staple fallback food source for the endangered Yunnan snub-nosed monkey (Rhinopithecus bieti) exert a critical influence on the survival of Yunnan snub-nosed monkey populations through their distribution dynamics. This study focused on the contiguous habitats of the Yunnan snub-nosed monkey in the southern Hengduan Mountains. By species distribution models (SDMs) and landscape pattern analysis, we investigated the changes in suitable habitats of lichens under four Representative Concentration Pathway (RCP) scenarios and their implications for the habitat utilization of the Yunnan snub-nosed monkey until 2050. The results indicate that the current suitable habitat for lichen spans approximately 16,821.96 km², with highly suitable habitats predominantly located in Deqin County and Weixi County. Altitude and vegetation type emerged as primary factors influencing lichen distribution. The overlap rate of suitable habitats between lichens and the Yunnan snub-nosed monkey is 72.24%. Furthermore, the Yunnan snub-nosed monkey exhibits a preference for selecting habitats characterized by the largest patch index (LPI) of lichen distribution. By 2050, the suitable habitat for lichen is projected to marginally increase in the southern Hengduan Mountains, particularly under the RCP 6.0 scenario, by 22.20% compared to the current expansion. However, both the suitable habitat and the LPI of lichen face potential decline within the habitat of the Yunnan snub-nosed monkey. Therefore, we recommend conducting a quantitative investigation into the correlation between the actual productivity of lichen radiata and the population dynamics of Yunnan snub-nosed monkey as a priority. This research will offer a more precise scientific foundation for conservation decision-making for Yunnan snub-nosed monkey. Full article

The Republic of Turkey established its first match factory in Sinop in 1929 but had to relocate it even before it was in operation due to severe structural damage caused by ground settlement. In July 1930, through his US-based firm the American–Turkish Investment Corporation (ATIC), the Swedish “Match King” Ivar Kreuger signed a contract with the Republic of Turkey to build and operate a factory in Büyükdere, Istanbul. By 1930, Kreuger had already established a match production monopoly in nearly every country in Europe and that year created a similar financial system for Turkey, gaining control of match production for 25 years. This article explains the events surrounding the establishment of his modern production facility in Turkey, with a particular focus on its engineering aspects. It details the strategically chosen location, the engineering solutions for the factory’s construction, its production lines, and what the country gained and lost from it. In order to determine the establishment and production processes of the facility, the authors examined domestic and foreign archival documents, firsthand news reports from the period, articles and theses, and all other available documents. After the contract was terminated by both parties, the Turkish government and ATIC, in May 1943, the factory continued its production and storage activities until May 1989. At that point, the factory and all its equipment were integrated into another existing facility in the İnegöl district of Bursa province. Almost all the buildings of the Büyükdere Match Factory were demolished, and the land was repurposed for a 450-bed regional hospital in 2012. In short, this article deploys the Büyükdere Match Factory as a case study to examine what Turkey gained and lost from the establishment and production processes of a modern industrial factory, enabled by US–Turkish collaboration, and equipped with the most advanced manufacturing and engineering technologies of the time. Full article

We conducted a three-year field study to evaluate the above-ground biomass yield, plant height, and tillering capacity of eight Sorghum bicolor (L.) Moench varieties under two contrasting soil conditions (heavy clay soil and sandy soil) with different water retention. At the Field Experimental Station Žabčice of Mendel University in Brno, Czech Republic, we assessed yield performance and yield stability across years and environments. We applied standard agronomic practices and recorded detailed soil and climatic data. Significant differences were found among varieties and between locations in terms of plant height and tillering. KWS SOLE showed the most stable yield (11.80–15.63 t ha⁻¹), while LATTE, KWS TARZAN, and KWS HANNIBAL achieved the highest average yields (up to 20.16 t ha⁻¹). Plant height showed a strong positive correlation with biomass yield. This relationship underscores plant height as a valuable trait for selecting sorghum varieties with improved productivity and drought resilience. Variations in tillering capacity and environmental conditions also significantly influenced yield outcomes, highlighting the complex interaction between genotype and environment. These findings offer practical insights for cultivar selection and breeding strategies that aim to enhance the performance of sorghum varieties under the variable climatic conditions of Central Europe. Full article

This study addresses the Capacitated Vehicle Routing Problem (CVRP) known to be NP-hard. In this problem, a set of customers with varying demands is considered. To solve the problem, routes were generated for several vehicles with identical capacity, which were responsible for delivering products to a set of geographically dispersed customers. The purpose of the problem is to minimize the total cost of all routes. This problem was solved by applying the metaheuristic Simulated Annealing (SA) and incorporating four different neighborhoods to improve the initial solution generated randomly. In the SA, a set of cooling factors is used. The best solution obtained by SA is refined by the use of Hill Climbing using a double neighborhood. The algorithm was tested with instances from the literature in order to measure its effectiveness in solution quality and execution time. We tested the approach with 106 instances from the literature and obtained the optimum in 93 instances. The average time in most instances was less than five minutes. Delivery companies can benefit from this approach. They only need to identify the depot, the clients, and the distance between locations, and this approach can be used with relative ease. Full article