Search Results (831)

Brassica napus L. is one of the world’s most important oilseed crops. Blackleg disease is a serious, yield-limiting factor in the cultivation of oilseed rape. Genetic resistance is primarily conferred by major resistance (R) genes. In this study, we analyzed the expression of the blackleg resistance genes Rlm3, Rlm4, and Rlm7 following inoculation with the Leptosphaeria maculans isolate using the RT-qPCR method. Additionally, we demonstrated and assessed their interactions. The results showed that, while Rlm3 was weakly induced, Rlm4 and Rlm7 displayed variable expression post-inoculation. The correlation between phenotypic and genotypic similarity was low. This suggests that transcriptional responses do not fully explain resistance patterns. Furthermore, significant main effects of the analyzed genes, as well as two- and three-way interactions, were indicated. These results support current knowledge of gene-mediated resistance to blackleg in oilseed rape. Full article

The increasing demand for sustainable agricultural practices has led researchers to explore alternative methods for controlling plant diseases and pests. Among these alternatives, essential oils (EOs) derived from various plant species have gained significant attention due to their broad-spectrum antimicrobial properties, which can be utilized in plant protection. Essential oils are volatile compounds that possess strong aromatic characteristics and are found in many medicinal and aromatic plants. They are known for their antifungal, antibacterial, and insecticidal activities, making them viable candidates for eco-friendly pest and disease management strategies. In this research, six essential oils—pine, patchouli, geranium, spruce, coriander, and eucalyptus oil—have been tested in vitro for controlling mycelium growth of Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria brassicicola, and Cylindrosporium concentricum. The study also covers experiments in controlling pollen beetle and cabbage seed weevil (laboratory trials). In greenhouse conditions, the phytotoxicity of EOs to oilseed rape (Brassica napus L.) and the effect of these substances on the control of cornflower (Centaurea cyanus) were also tested. The results obtained indicate a large diversity of different essential oils in terms of their action on pathogens, pests, weeds, and winter rapeseed. Differences in their effectiveness were also found, depending on the applied dose. Full article

Biochar application can effectively immobilize Cadmium (Cd) in soil. However, it is largely unknown how the biogeochemical processes of sulfur (S) in biochar affect Cd fixation under conditions of decreasing pe + pH. Using two field-contaminated paddy soils with different Cd concentrations (Shangyu (SY) 0.56 mg kg⁻¹ and Tongling (TL) 2.32 mg kg⁻¹), and rape straw biochars with low S (LB) and high S (HB) contents, we investigated how S-rich biochar regulates Cd solubility in paddy soils that were incubated anaerobically for 40 d. The soluble and extractable Cd content decreased as pe + pH decreased with flooding, and showed a steady trend by day 20. However, Cd was immobilized through different mechanisms in TL and SY soil. The rapid decrease in pe + pH in TL soil enhanced the involvement of S in Cd immobilization and Fe transformation. In SY soil, the delayed reduction in SO₄²⁻ promoted Cd adsorption onto amorphous Fe oxides. During this process, the liming effect of biochar is critical for Cd immobilization in soil. Furthermore, biochar might promote the biogeochemical processes of S and Fe transformation, thereby enhancing Cd fixation in soils. Full article

Efficient pesticide application remains a critical resource-management challenge in modern agriculture, where limited spray penetration reduces treatment efficacy, wastes chemical inputs, and increases environmental losses. This study quantified the effect of air-assisted spraying (AAS) on droplet deposition in two contrasting field crops, oilseed rape and wheat. Field trials were conducted using a sprayer equipped with an adjustable airflow module, and spray coverage was measured with water-sensitive papers at multiple canopy heights and orientations. In oilseed rape, AAS improved deposition on front-facing and top surfaces in the lower canopy, for example, increasing top-surface coverage at 90 cm from 53.4% to 65.5% at 6 km∙h⁻¹, indicating more uniform distribution and enhanced penetration. In wheat, which typically exhibits a more open canopy structure compared to oilseed rape, AAS effects were smaller and less consistent, with the greatest gain on front-facing lower surfaces (from 13.3% to 21.9% at 7 km∙h⁻¹). Although drift was not measured in this experiment, previous studies using the same sprayer prototype demonstrated measurable reductions, supporting the environmental relevance of improved deposition. These results highlight the role of canopy architecture in determining AAS performance and underscore the technology’s potential to reduce pesticide inputs, minimize off-target losses, and improve the resource efficiency of crop protection in line with EU Farm to Fork objectives. Full article

This study investigates the biocontrol potential of Trichoderma asperellum and Coniothyrium minitans against the pathogen Sclerotinia sclerotiorum, which causes yield losses in many plants, including oilseed rape (Brassica napus) cultivation. This research emphasizes the promising alternative of hybrid control, specifically using T. asperellum and C. minitans in strategy with synthetic fungicides. In vitro experiments demonstrated that T. asperellum effectively inhibited S. sclerotiorum mycelial growth, especially when combined with synthetic fungicides such as azoxystrobin. Field trials conducted over two years revealed that pre-sowing applications of T. asperellum and C. minitans, followed by fungicide treatments during the flowering stage, significantly reduced plant infection rates and improved both yield and seed quality across different oilseed rape cultivars. The results indicated an efficacy range of 81% to 100% in controlling the pathogen and highlighted the synergistic effects of combining biological and chemical controls. Overall, the research findings support the integration of T. asperellum and C. minitans into sustainable agricultural practices for oilseed rape, offering a viable strategy to enhance disease management while reducing reliance on chemical fungicides. This research underscores the importance of adopting innovative biocontrol approaches to improve crop health and productivity. Full article

Forage rape (Brassica napus L.) is increasingly becoming a valued forage choice in livestock production. However, research on the application of whole-plant rape silage (including pod shells) in goats remains limited. To evaluate the effects of whole-plant rape silage on goat growth performance, meat quality, antioxidant capacity, and intestinal health, a 90-day feeding trial was performed using 36 healthy 6-month-old Chongming white goats. The goats were fed ad libitum and divided into two groups: a control group (CON, n = 18) fed corn straw silage and a treatment group (TRT, n = 18) fed a diet containing a 1:1 mixture of whole-plant rape silage and corn straw silage. Results showed that a 50% substitution with whole-plant rape silage increased carcass weight (p = 0.005), enhanced total antioxidant capacity (p < 0.01) in plasma, reduced bitter amino acids (such as leucine, isoleucine and phenylalanine; p < 0.05) in muscle, promoted intestinal villi proliferation (p < 0.05), and increased the abundance of beneficial bacteria involved in carbohydrate metabolism (e.g., Family_XIII_AD3011_group; p = 0.028) and propionic acid metabolism (e.g., Phascolarctobacterium; p = 0.026). In conclusion, our findings demonstrated that whole-plant rape silage can serve as a viable alternative to corn straw silage for Chongming white goats. Full article

There is little research exploring the grey area of sexual violence (SV), considered in the literature as being a more subtle manifestation of SV, and therefore tending to be trivialized, legitimized, and normalized by society. This study aimed to compare students’ perceptions of the grey area of SV based on the gender of those involved, in a cis-hetero context, as well as potential sex differences in these perceptions. A vignette methodology was employed to gain valuable insights into the topic. The sample consisted of 164 university students living in Portugal, 71.3% (n = 117) female, with an average age of 23 (SD = 5.84). The thematic analysis revealed a central theme, Severity, reflecting participants’ perceptions of the seriousness of sexual violence within the “grey area”. This theme is expressed through four sub-themes: Attribution of severity, referring to how seriousness is assigned depending on context and beliefs; Identifying sexual violence, highlighting difficulties in recognizing certain behaviors as abusive; Frequency, capturing perceptions of how often such situations occur; and Report, addressing the barriers and facilitators to formal reporting. Our results indicated that while some participants minimized the scenario, the majority of the sample considered the situation as somewhat or very serious. Only few participants trivialized subtle forms of SV, perceiving incidents without overt physical force as less severe. Notably, sex differences emerged, despite being in the minority of the sample, female participants were more inclined to recognize these behaviors as abusive and to view the allegations as credible, whereas male participants tended to downplay the severity. Full article

Organic amendments application has been proposed as an efficient method for remediation of heavy metals-contaminated soils. This study evaluated the performance of the water-insoluble organic material polyvinylpolypyrrolidone on decontaminating water and soil polluted by heavy metals Cu, Cd and Zn via batch trials, soil incubation and pot experiments with oilseed rape. The adsorption process of Cu, Cd and Zn by polyvinylpolypyrrolidone included a rapid step which achieved 92%, 76% and 87% of adsorption capacities within 10 min, followed with a slow step before reaching equilibrium which varied from 4 to 24 h among the three heavy metals. The maximum adsorption capacities were 327, 330 and 186 mg g⁻¹ for Cu, Cd and Zn, respectively. With application doses of polyvinylpolypyrrolidone ranging from 10 to 60 g kg⁻¹, the DTPA-extracted Cu, Cd and Zn showed 59–96%, 27–93% and 13–83% reduction compared to no addition. Moreover, the uptake of Cu, Cd and Zn by oilseed rape were significantly inhibited with polyvinylpolypyrrolidone amendments, and the effects improved with the accrual of polyvinylpolypyrrolidone. Intriguingly, the application of polyvinylpolypyrrolidone showed insignificant influences on nutrients taken up by oilseed rape. Results of the present study indicated that polyvinylpolypyrrolidone is a promising organic amendment for heavy metal (Cu, Cd and Zn) stabilization in polluted water and soil. Full article

(1) Background: Sclerotinia sclerotiorum is the main causal agent of Sclerotinia stem rot in rapeseed, while the related species S. subarctica has also been reported. However, its prevalence and impact in Germany remain unclear. Understanding the pathogenicity and fungicide sensitivity of Sclerotinia spp. is important for effective and sustainable disease management. (2) Methods: Isolates were collected from symptomatic rapeseed plants across Germany. Molecular identification was performed via ITS rRNA sequencing. Pathogenicity was assessed by stem inoculation of five rapeseed cultivars at the flowering stage. Fungicide sensitivity was tested in vitro against seven active substances, including azoles, boscalid, azoxystrobin, and fludioxonil. (3) Results: All isolates were identified as S. sclerotiorum; S. subarctica was not detected. Of the tested isolates, 23 showed low aggressiveness (relative lesion length < 15% of total plant length), 29 were moderately aggressive (15–20%), and 10 were highly aggressive (>20%). Azole fungicides were highly effective (EC₅₀ < 1.6 μg a.s. mL⁻¹), while reduced sensitivity was observed for boscalid, azoxystrobin, and fludioxonil (EC₅₀ > 4.0). (4) Conclusions: This study provides insight into the molecular identity, pathogenicity, and fungicide sensitivity of Sclerotinia isolates. The observed variability in aggressiveness and mycelial growth to fungicide emphasize the need for integrated management strategies to ensure Sclerotinia stem rot control. Full article

Benzodiazepines are psychoactive drugs with wide clinical applications. Unfortunately, due to their sedative effects, benzodiazepines are frequently used as date rape drugs or in drug-facilitated crimes. Considering the electroactive nature of benzodiazepines and the unique advantages of electrochemical techniques, this review presents a critical discussion of the state of the art of benzodiazepine electroanalysis. Aspects related to sample preparation as well as electrodes (from mercury electrodes to bare or modified solid electrodes and to disposable sensors) and techniques (mainly voltammetry) used for the quantification of benzodiazepines in different matrices (pharmaceuticals, body fluids, alcoholic and soft drinks) were discussed. Considering the actual achievements in the field, some general suggestions for possible further research were given. Full article

Climate change has caused considerable uncertainty to oilseed rape production. However, the climatic suitability for oilseed rape cultivation and its future changing trend remain unclear, specifically in Jiangsu Province—a major oilseed rape producing-region in China. Based on the past 50 years (1969–2018) of daily meteorological data from 13 meteorological stations in the province, this study established a climate suitability assessment model for oilseed rape cultivation. Temperature, precipitation, and sunlight were comprehensively analyzed, with suitable zones delineated through GIS spatial analysis and the natural break method. With the incorporation of SSP2-4.5 climatic scenario simulation data, the study projected the evolving trends of oilseed rape cultivation climatic suitability zones from 2024 to 2050 in the province. The findings reveal that over the past five decades, the climatic suitability for oilseed rape planting in the province has demonstrated the following patterns: temperature suitability increased by 0.02 per decade, precipitation suitability declined by −0.01 per decade, sunlight suitability decreased by −0.01 per decade, and comprehensive suitability rose by 0.01 per decade. High climatic suitability with the index of 0.80–1.00 was predominantly clustered in the central region, while moderate suitability zones with the index of 0.50–0.80 were mainly found in its northern and southern regions. Unsuitable zones with the index of 0.00–0.50 were mainly confined to the northern and southern extremities of the province. Under future climate scenarios, oilseed rape planting suitability is projected to improve significantly, with highly suitable zones expanding, particularly into the central and parts of the northern Jiangsu. Moderately suitable zones also will be extended, including potential areas such as the parts of Lianyungang and Wuxi. Unsuitable zones will be reduced, with only limited areas like southern Wuxi retaining lower suitability. Future temperature increases in Lianyungang are expected to be in favor of oilseed rape production. However, excessive precipitation in the southern region will require enhanced drainage measures. Improved temperature and precipitation conditions in Xuzhou are anticipated to boost the climatic suitability. Overall, oilseed rape planting climatic factors in the central and northern regions are projected to improve, enabling production expansion, while the southern region will face the challenge of excessive precipitation in Jiangsu Province. Full article

Sclerotinia sclerotiorum, a devastating phytopathogenic fungus with global distribution, exhibits a broad host range encompassing over 700 plant species. Sclerotinia stem rot caused by this pathogen poses a significant threat to sustainable oilseed rape production. Coniothyrium minitans, a mycoparasite of S. sclerotiorum, is a promising biological control agent against this devastating disease. C. minitans-based formulations have been commercially developed for field application. A transcriptomic analysis revealed significant upregulation of the chitinase-encoding gene CmCH1 in C. minitans during interaction with S. sclerotiorum. Knockout of either CmCH1 or another chitinase-encoding gene CmCH10 in C. minitans did not markedly affect the mycelial growth, development, and parasitism of S. sclerotiorum. However, knockout CmCH1 and CmCH10 simultaneously resulted in reduced growth rate, impaired protoplast release, enhanced cell wall integrity, and diminished mycoparasitic capability. These results indicate that CmCH1 and CmCH10 collectively influence remodeling of the cell wall in C. minitans and its mycoparasitic activity. Full article

Fatty acids (FAs) are vital for human nutrition and are classified into three categories (saturated, unsaturated, and trans). FAs have different physiological effects and can contribute to health problems in different ways. By-products from the oil industry are rich in bioactive compounds. These make them useful for further utilization in food formulation. There is a quantity of residual oil in the oilcake. Analysis of the fatty acid composition shows that unsaturated fatty acids are predominant. The predominant fatty acids in oilcakes are arachidic (sunflower), oleic, elaidic (flax), linoleic (LA), and linolelaidic (hemp, rape, and sesame) acids. The favorable and ideal (within the regulatory recommendations) results for the n-6/n-3 ratios of 3:1 indicate the high nutritional profile with beneficial effects for the human body of the oilcakes. The hypocholesterolemic/hypercholesterolemic for all samples ranged from 4.52 to 116.06, while atherogenicity and thrombogenicity indexes ranged from 0.01 to 0.3. This is in line with the favorable values found in the literature benchmarks. Full article

Xylazine (XYL), an FDA-approved veterinary tranquilizer, is being abused both as an opioid adulterant in a street-drug known as “Tranq-dope” and as a date rape drug. Given its now nearly ubiquitous use with fentanyl and fentanyl derivatives across the globe, XYL has become a primary target for researchers seeking to develop portable and cost-effective sensors for its detection. Electrochemical sensors based on the oxidation of XYL, while useful, have limitations due to certain interferents and inherent electrode fouling that render the approach less reliable, especially in certain sample matrices. In this work, modified electrode platforms incorporating layers of multi-walled carbon nanotubes for sensitivity along with semi-permeable polyurethane (PU) layers and host–guest chemistry using β-cyclodextrin for selectivity are deployed for XYL detection using complementary adsorptive cathodic stripping analysis. The modified electrode sensors are optimized to minimize high potentials and maintain fouling resistant capabilities and investigated to better understand the function of the PU layer. The use of adsorptive cathodic stripping differential pulse voltammetry indirectly indicates the presence and concentration of XYL within complex sample media (beverages and synthetic urine). When used in this manner, the modified electrodes exhibited an overall average sensitivity of ~35 (±9) nA/μM toward XYL with a limit of quantification of <10 ppm, while also offering adaptability for the analysis of XYL in different types of samples. By expanding the capability of these XYL sensors, this study represents another facet of tool development for use by medical professionals, first-responders, forensic investigators, and drug-users to limit exposure and help stem the dangerous and illegal use of XYL. Full article

Background: Sexual violence poses a significant challenge to European lawmakers, impacting the victim’s physical and psychological health. This study examines sexual violence legislation across EU member states, Switzerland, and the UK, analyzing similarities, differences, challenges, and potential solutions for effective policy development. The research was motivated by the adoption of EU Directive 2024/1385. Methods: This study analyzes sexual violence legislation across European countries in a comparative and qualitative way, highlighting differences, commonalities, and the potential for uniform regulation. The data were collected from the literature published between 2015 and 2024, focusing the EU member states, Switzerland, and the United Kingdom. Results: The examination of the norms governing sexual offenses in various European countries revealed significant differences in legislative frameworks, reflecting diverse cultural, ethical, and legal perspectives. Conclusions: Despite European countries sharing the goal of protecting victims and combating sexual violence, there are significant legislative disparities. Key recommendations include enhancing EU member state cooperation, implementing joint training programs, developing a specific EU directive, and creating coordinated prevention and education programs. While respecting national legal diversity, a unified approach is needed for effective prevention and prosecution of sexual violence across Europe. Full article